{"id":"https://openalex.org/W1513464976","doi":"https://doi.org/10.1016/s0021-9258(19)47247-5","title":"Amino Acid Residues Stabilizing a Bacillus \u03b1-Amylase against Irreversible Thermoinactivation","display_name":"Amino Acid Residues Stabilizing a Bacillus \u03b1-Amylase against Irreversible Thermoinactivation","publication_year":1989,"publication_date":"1989-11-01","ids":{"openalex":"https://openalex.org/W1513464976","doi":"https://doi.org/10.1016/s0021-9258(19)47247-5","mag":"1513464976","pmid":"https://pubmed.ncbi.nlm.nih.gov/2808401"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)47247-5","doi":"https://doi.org/10.1016/s0021-9258(19)47247-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)47247-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)47247-5","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063909273","display_name":"Yasuh\u00edro Suzuki","orcid":"https://orcid.org/0000-0002-1868-1938","observed_orcids":["https://orcid.org/0000-0002-1868-1938"]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Y Suzuki","raw_affiliation_strings":["Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Japan","institution_ids":["https://openalex.org/I60134161"]}]},{"author_position":"middle","author":{"id":null,"display_name":"N Ito","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"N Ito","raw_affiliation_strings":["Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464 Japan","institution_ids":["https://openalex.org/I60134161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015488402","display_name":"Toshifumi Yuuki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"T Yuuki","raw_affiliation_strings":["Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464 Japan","institution_ids":["https://openalex.org/I60134161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076286709","display_name":"Hideo Yamagata","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"H Yamagata","raw_affiliation_strings":["Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464 Japan","institution_ids":["https://openalex.org/I60134161"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088081540","display_name":"Shigezo Udaka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I60134161","display_name":"Nagoya University","ror":"https://ror.org/04chrp450","country_code":"JP","type":"education","lineage":["https://openalex.org/I60134161"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"S Udaka","raw_affiliation_strings":["Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464 Japan","institution_ids":["https://openalex.org/I60134161"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I60134161"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.8009,"has_fulltext":false,"cited_by_count":125,"citation_normalized_percentile":{"value":0.93303571,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"264","issue":"32","first_page":"18933","last_page":"18938"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8217999935150146,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.8217999935150146,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.16419999301433563,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-amylase","display_name":"alpha-amylase","score":0.8960000276565552},{"id":"https://openalex.org/keywords/bacillus-licheniformis","display_name":"Bacillus licheniformis","score":0.9879999756813049},{"id":"https://openalex.org/keywords/bacillus-amyloliquefaciens","display_name":"Bacillus amyloliquefaciens","score":0.9990000128746033},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.9369999766349792},{"id":"https://openalex.org/keywords/fusion-genes","display_name":"fusion genes","score":0.8019999861717224},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.9760000109672546},{"id":"https://openalex.org/keywords/amino-acid-residues","display_name":"amino acid residues","score":0.7889999747276306},{"id":"https://openalex.org/keywords/optimal-temperature","display_name":"optimal temperature","score":0.8040000200271606},{"id":"https://openalex.org/keywords/enzyme-stability","display_name":"enzyme stability","score":0.5879999995231628}],"concepts":[{"id":"https://openalex.org/C170835558","wikidata":"https://www.wikidata.org/wiki/Q17153","display_name":"Amylase","level":3,"score":0.6656016111373901},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6243156790733337},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5335531830787659},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5248789191246033},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.23089587688446045}],"mesh":[{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001407","descriptor_name":"Bacillus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001407","descriptor_name":"Bacillus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015345","descriptor_name":"Oligonucleotide Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)47247-5","doi":"https://doi.org/10.1016/s0021-9258(19)47247-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)47247-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:2808401","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2808401","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)47247-5","doi":"https://doi.org/10.1016/s0021-9258(19)47247-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)47247-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1479683678","https://openalex.org/W1485865656","https://openalex.org/W1531019289","https://openalex.org/W1542344248","https://openalex.org/W1546783991","https://openalex.org/W1601687245","https://openalex.org/W1775749144","https://openalex.org/W1845136592","https://openalex.org/W1851792052","https://openalex.org/W1853318271","https://openalex.org/W1874832326","https://openalex.org/W1902777381","https://openalex.org/W1966302520","https://openalex.org/W1994017148","https://openalex.org/W2028622989","https://openalex.org/W2034630918","https://openalex.org/W2035283686","https://openalex.org/W2049390259","https://openalex.org/W2050499271","https://openalex.org/W2070184351","https://openalex.org/W2075354188","https://openalex.org/W2132417094","https://openalex.org/W2138270253","https://openalex.org/W2171548073","https://openalex.org/W7097930909"],"related_works":["https://openalex.org/W1479683678","https://openalex.org/W637136226","https://openalex.org/W7099311295","https://openalex.org/W2020569900","https://openalex.org/W2185058449","https://openalex.org/W2399181252","https://openalex.org/W7052483297","https://openalex.org/W4321792573","https://openalex.org/W1980526190","https://openalex.org/W2138435059"],"abstract_inverted_index":{"The":[0],"alpha-amylase":[1,45],"of":[2,16,46,54,61,101,120,134,137,142,151,171,205],"Bacillus":[3,47],"licheniformis":[4],"(BLA)":[5],"is":[6,19,38,63],"stable":[7],"and":[8,31,83,107,144,149,155,180],"active":[9],"at":[10,24,66,219],"high":[11],"temperature.":[12],"More":[13],"than":[14,222],"80%":[15,57],"its":[17,36],"activity":[18,37],"retained":[20],"after":[21],"heat":[22],"treatment":[23],"90":[25,67],"degrees":[26,40,68,208,224],"C":[27],"for":[28,35,79,88,153,158,168],"30":[29],"min,":[30],"the":[32,44,50,76,80,97,108,118,130,138,169,172,177,184,194],"optimum":[33,200],"temperature":[34,199],"80-85":[39],"C.":[41,69,225],"In":[42],"contrast,":[43],"amyloliquefaciens":[48],"(BAA),":[49],"amino":[51,98],"acid":[52,99,157],"sequence":[53,100],"which":[55],"shows":[56],"homology":[58],"with":[59],"that":[60,204,211],"BLA,":[62],"rapidly":[64],"inactivated":[65],"Various":[70],"chimeric":[71],"genes":[72,78],"were":[73,86,114,163],"constructed":[74],"from":[75],"structural":[77],"two":[81],"enzymes,":[82],"their":[84],"products":[85],"analyzed":[87],"stability":[89],"as":[90,187,189,201,203],"to":[91,116,165,190,216],"irreversible":[92,191],"thermoinactivation.":[93,192],"Two":[94],"regions":[95],"in":[96,128,146,160],"BLA":[102,188],"comprising":[103],"Gln178":[104],"(region":[105,111],"I)":[106],"255th-270th":[109],"residues":[110],"II),":[112],"respectively,":[113],"shown":[115,164],"determine":[117],"thermostability":[119,186],"BLA.":[121],"Region":[122],"I":[123,148],"plays":[124],"a":[125,198],"major":[126],"role":[127],"determining":[129],"thermostability.":[131,173],"By":[132],"means":[133],"site-directed":[135],"mutagenesis":[136],"BAA":[139,206],"gene,":[140],"deletion":[141,179],"Arg176":[143],"Gly177":[145],"region":[147,161],"substitutions":[150,181],"alanine":[152],"Lys269":[154],"aspartic":[156],"Asn266":[159],"II":[162],"be":[166],"responsible":[167],"enhancement":[170],"Mutant":[174],"BAAs":[175,196],"containing":[176],"above":[178],"showed":[182,197],"almost":[183],"same":[185],"Nevertheless,":[193],"mutant":[195],"low":[202],"(65":[207],"C),":[209],"indicating":[210],"they":[212],"are":[213],"still":[214],"susceptible":[215],"reversible":[217],"inactivation":[218],"temperatures":[220],"higher":[221],"65":[223]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}