{"id":"https://openalex.org/W1507399535","doi":"https://doi.org/10.1016/s0021-9258(19)68305-5","title":"A homogeneous cyclic CMP phosphodiesterase hydrolyzes both pyrimidine and purine cyclic 2':3'- and 3':5'-nucleotides.","display_name":"A homogeneous cyclic CMP phosphodiesterase hydrolyzes both pyrimidine and purine cyclic 2':3'- and 3':5'-nucleotides.","publication_year":1982,"publication_date":"1982-01-01","ids":{"openalex":"https://openalex.org/W1507399535","doi":"https://doi.org/10.1016/s0021-9258(19)68305-5","mag":"1507399535","pmid":"https://pubmed.ncbi.nlm.nih.gov/6274851"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)68305-5","doi":"https://doi.org/10.1016/s0021-9258(19)68305-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)68305-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)68305-5","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039994535","display_name":"David M. Helfman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D.M. Helfman","raw_affiliation_strings":["From the Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108902860","display_name":"J.F. Kuo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J.F. Kuo","raw_affiliation_strings":["From the Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322","institution_ids":["https://openalex.org/I150468666"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I150468666"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.9838,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.70418492,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"257","issue":"2","first_page":"1044","last_page":"1047"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":0.926800012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":0.926800012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.06080000102519989,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cyclic-nucleotide-hydrolysis","display_name":"cyclic nucleotide hydrolysis","score":0.8180000185966492},{"id":"https://openalex.org/keywords/pyrimidine-nucleotides","display_name":"pyrimidine nucleotides","score":0.8889999985694885},{"id":"https://openalex.org/keywords/purine-nucleotides","display_name":"purine nucleotides","score":1.0},{"id":"https://openalex.org/keywords/5-nucleotides","display_name":"5'-nucleotides","score":0.9330000281333923},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.6830000281333923},{"id":"https://openalex.org/keywords/catalytic-site","display_name":"catalytic site","score":0.5429999828338623}],"concepts":[{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.809878945350647},{"id":"https://openalex.org/C2776476023","wikidata":"https://www.wikidata.org/wiki/Q188261","display_name":"Purine","level":3,"score":0.7434160113334656},{"id":"https://openalex.org/C2779321679","wikidata":"https://www.wikidata.org/wiki/Q207722","display_name":"Pyrimidine","level":2,"score":0.7050382494926453},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6288413405418396},{"id":"https://openalex.org/C66882249","wikidata":"https://www.wikidata.org/wiki/Q169336","display_name":"Homogeneous","level":2,"score":0.5979025363922119},{"id":"https://openalex.org/C62826618","wikidata":"https://www.wikidata.org/wiki/Q424304","display_name":"Phosphodiesterase","level":3,"score":0.5683296918869019},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.4801018536090851},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.45737743377685547},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.41379380226135254},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2234528660774231},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.12005773186683655},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C114614502","wikidata":"https://www.wikidata.org/wiki/Q76592","display_name":"Combinatorics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D009712","descriptor_name":"Nucleotides, Cyclic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010727","descriptor_name":"Phosphoric Diester Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D063309","descriptor_name":"2',3'-Cyclic Nucleotide 3'-Phosphodiesterase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0021-9258(19)68305-5","doi":"https://doi.org/10.1016/s0021-9258(19)68305-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)68305-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:6274851","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6274851","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:repository.cshl.edu:39114","doi":null,"is_oa":false,"landing_page_url":"https://repository.cshl.edu/id/eprint/39114/","pdf_url":null,"source":{"id":"https://openalex.org/S4306402288","display_name":"Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Paper"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)68305-5","doi":"https://doi.org/10.1016/s0021-9258(19)68305-5","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)68305-5","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W10073932","https://openalex.org/W46393137","https://openalex.org/W1517810493","https://openalex.org/W1542630845","https://openalex.org/W1602784471","https://openalex.org/W1603886610","https://openalex.org/W1970263693","https://openalex.org/W1981135378","https://openalex.org/W1987391235","https://openalex.org/W1993758564","https://openalex.org/W2013877717","https://openalex.org/W2023096507","https://openalex.org/W2052899088","https://openalex.org/W2070339573","https://openalex.org/W2128635872","https://openalex.org/W2299342448","https://openalex.org/W2415573782"],"related_works":["https://openalex.org/W7029151698","https://openalex.org/W2054991262","https://openalex.org/W2035397040","https://openalex.org/W2029257278","https://openalex.org/W1517810493","https://openalex.org/W1544383220","https://openalex.org/W2065967725","https://openalex.org/W2088355827","https://openalex.org/W2110058673","https://openalex.org/W2095413102"],"abstract_inverted_index":{"A":[0],"homogeneous":[1],"preparation":[2],"of":[3,28,38,45,53,59,71,77,115,117,170,182,209,214],"cyclic":[4,33,39,60,63,72,87,96,118,121,125,129,136,142,145,149,156,160,187,215],"CMP":[5,122,146],"phosphodiesterase":[6,202],"(Helfman,":[7],"D.":[8],"M.,":[9,11],"Shoji,":[10],"and":[12,31,35,48,79,94,185,189],"Kuo,":[13],"J.":[14,17],"F.":[15],"(1981)":[16],"Biol.":[18],"Chem.":[19],"256,":[20],"6327-6334)":[21],"was":[22,89,120,144],"found":[23],"to":[24,108,135,155,204],"catalyze":[25],"the":[26,43,54,82,102,106,174,180,195,199],"hydrolysis":[27,70,116,181],"both":[29,46,183],"pyrimidine":[30,93,184],"purine":[32,95,186],"2':3'-":[34,188],"3':5'-nucleotides.":[36,190],"Hydrolysis":[37,58],"2':3'-nucleotides":[40,119],"resulted":[41],"in":[42,179],"formation":[44],"2'-":[47,78],"3'-nucleotides,":[49,80],"although":[50],"relative":[51,113],"amounts":[52,76],"products":[55],"were":[56,98],"variable.":[57],"2':3'-CMP":[61],"or":[62,133,153],"2':3'-UMP":[64],"yielded":[65],"predominantly":[66],"3'-nucleotides.":[67],"In":[68],"contrast,":[69],"2':3'-AMP":[73],"produced":[74],"equal":[75,134,154],"while":[81],"major":[83],"product":[84],"formed":[85],"from":[86],"2':3'-GMP":[88],"2'-nucleotide.":[90],"When":[91],"conventional":[92],"3:5'-nucleotides":[97],"used":[99],"as":[100],"substrates,":[101],"enzyme":[103,175,197],"hydrolyzed":[104],"specifically":[105],"3'-bond":[107],"yield":[109],"only":[110],"5'-nucleotides.":[111],"The":[112],"rate":[114],"greater":[123,127,131,147,151,158],"than":[124,128,132,148,152,159],"UMP":[126,150],"GMP":[130],"AMP,":[137],"respectively,":[138],"whereas":[139],"that":[140,194,206],"for":[141],"3':5'-nucleotides":[143],"AMP":[157],"GMP,":[161],"respectively.":[162],"Furthermore,":[163],"kinetic":[164],"analysis":[165],"suggested":[166],"a":[167,212],"single":[168],"species":[169],"catalytic":[171],"site":[172],"on":[173],"may":[176],"be":[177],"involved":[178],"These":[191],"findings":[192],"indicate":[193],"present":[196],"is":[198,207],"first":[200],"multifunctional":[201],"reported":[203],"date":[205],"capable":[208],"hydrolyzing":[210],"such":[211],"diversity":[213],"nucleotides.":[216]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}