{"id":"https://openalex.org/W1506563788","doi":null,"title":"Mapping of DNA alkylation sites induced by aziridinylbenzoquinones in human cells by ligation-mediated polymerase chain reaction.","display_name":"Mapping of DNA alkylation sites induced by aziridinylbenzoquinones in human cells by ligation-mediated polymerase chain reaction.","publication_year":1994,"publication_date":"1994-04-01","ids":{"openalex":"https://openalex.org/W1506563788","mag":"1506563788","pmid":"https://pubmed.ncbi.nlm.nih.gov/8137269"},"language":"en","primary_location":{"id":"pmid:8137269","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8137269","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5078231565","display_name":"Chong-Soon Lee","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C S Lee","raw_affiliation_strings":["School of Pharmacy, University of Southern California, Los Angeles 90033"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Pharmacy, University of Southern California, Los Angeles 90033","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048312997","display_name":"Gerd P. Pfeifer","orcid":"https://orcid.org/0000-0002-5080-9604","observed_orcids":["https://orcid.org/0000-0002-5080-9604"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G P Pfeifer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5112057559","display_name":"Neil W. Gibson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"N W Gibson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.03399187,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"54","issue":"7","first_page":"1622","last_page":"6"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12356","display_name":"Bioactive Compounds and Antitumor Agents","score":0.9520999789237976,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12356","display_name":"Bioactive Compounds and Antitumor Agents","score":0.9520999789237976,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13105","display_name":"Synthesis and pharmacology of benzodiazepine derivatives","score":0.014000000432133675,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.011699999682605267,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-alkylation","display_name":"DNA alkylation","score":0.8289999961853027},{"id":"https://openalex.org/keywords/aziridinylbenzoquinone","display_name":"aziridinylbenzoquinone","score":0.9670000076293945},{"id":"https://openalex.org/keywords/bioreductive-activation","display_name":"bioreductive activation","score":0.7960000038146973},{"id":"https://openalex.org/keywords/colon-cancer-cells","display_name":"colon cancer cells","score":0.8640000224113464},{"id":"https://openalex.org/keywords/ht-29-cells","display_name":"HT-29 cells","score":0.7440000176429749}],"concepts":[{"id":"https://openalex.org/C164361826","wikidata":"https://www.wikidata.org/wiki/Q177604","display_name":"Alkylation","level":3,"score":0.7410603761672974},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6287820935249329},{"id":"https://openalex.org/C179437574","wikidata":"https://www.wikidata.org/wiki/Q15089798","display_name":"Ligation","level":2,"score":0.5830259919166565},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5822275280952454},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.5782248973846436},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5339919924736023},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5267250537872314},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5010888576507568},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.46794548630714417},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.4398612976074219},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.42228758335113525},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3827182948589325},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33367788791656494},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.32828229665756226},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1733863353729248},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000478","descriptor_name":"Alkylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000970","descriptor_name":"Antineoplastic Agents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001388","descriptor_name":"Aziridines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003110","descriptor_name":"Colonic Neoplasms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005091","descriptor_name":"Exons","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010735","descriptor_name":"Phosphoglycerate Kinase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016158","descriptor_name":"Genes, p53","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016227","descriptor_name":"Benzoquinones","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016660","descriptor_name":"NAD(P)H Dehydrogenase (Quinone)","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016660","descriptor_name":"NAD(P)H Dehydrogenase (Quinone)","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:8137269","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8137269","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer research","raw_type":"Journal Article"},{"id":"mag:1506563788","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8137269","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.504800021648407,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8053201134","display_name":"ANTICANCER DRUG INDUCED DNA DAMAGE AND REPAIR","funder_award_id":"R01CA059537","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2141079267","https://openalex.org/W2289324486","https://openalex.org/W2015687520","https://openalex.org/W7097711748","https://openalex.org/W2046363941","https://openalex.org/W2105476437","https://openalex.org/W2090029558","https://openalex.org/W2021236154","https://openalex.org/W2075540239","https://openalex.org/W2150531682"],"abstract_inverted_index":{"Diaziridinylbenzoquinones":[0],"such":[1],"as":[2],"3,6-diaziridinyl-1,4-benzoquinone":[3],"(DZQ)":[4],"and":[5,33,63,78,86,99,129,151,178],"its":[6],"2,5-methyl":[7],"analog":[8],"(MeDZQ)":[9],"require":[10],"bioreductive":[11],"activation":[12],"in":[13,35,54,67,113,138,157,175,183],"order":[14],"to":[15,82,106,154,180],"elicit":[16],"their":[17],"cytotoxic":[18],"activities.":[19],"In":[20,71],"this":[21,52],"study,":[22],"we":[23],"have":[24,50],"mapped":[25],"the":[26,41,72,93,139,158],"intracellular":[27],"alkylation":[28,147],"sites":[29],"induced":[30,148],"by":[31,103,149],"DZQ":[32,77,98,150,177],"MeDZQ":[34,79,100,152,179],"a":[36,144],"single":[37],"copy":[38],"gene":[39],"at":[40],"nucleotide":[42,94],"level":[43],"using":[44],"ligation-mediated":[45],"polymerase":[46],"chain":[47],"reaction.":[48],"We":[49],"performed":[51],"analysis":[53],"two":[55],"human":[56],"colon":[57],"carcinoma":[58],"cells,":[59],"one":[60,64],"proficient":[61],"(HT-29)":[62],"deficient":[65],"(BE)":[66],"DT-diaphorase":[68],"(DTD)":[69],"activity.":[70],"DTD-proficient":[73,159],"HT-29":[74,160],"cell":[75,142],"line,":[76],"were":[80],"found":[81],"alkylate":[83],"both":[84],"5'-(A/T)G(C)-3'":[85],"5'-(A/T)A-3'":[87],"sequences.":[88],"This":[89,164],"is":[90],"consistent":[91],"with":[92],"preferences":[95],"observed":[96,156],"when":[97],"are":[101],"activated":[102],"purified":[104],"DTD":[105,171],"reactive":[107],"metabolites":[108],"capable":[109],"of":[110,146],"alkylating":[111],"DNA":[112],"vitro":[114],"(C-S.":[115],"Lee,":[116],"J.":[117,123],"A.":[118],"Hartley,":[119],"M.":[120],"D.":[121,125,127],"Berardini,":[122],"Butler,":[124],"Siegel,":[126],"Ross,":[128],"N.":[130],"W.":[131],"Gibson.":[132],"Biochemistry,":[133],"31:":[134],"3019-3025,":[135],"1992).":[136],"Surprisingly":[137],"DTD-deficient":[140],"BE":[141],"line":[143],"pattern":[145],"similar":[153],"that":[155,166],"cells":[161],"was":[162],"observed.":[163],"suggests":[165],"reductive":[167],"enzymes":[168],"other":[169],"than":[170],"can":[172],"be":[173],"involved":[174],"activating":[176],"DNA-reactive":[181],"species":[182],"vivo.":[184]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}