{"id":"https://openalex.org/W1504713675","doi":"https://doi.org/10.1016/s0021-9258(19)45522-1","title":"Wheat Germ Agglutinin","display_name":"Wheat Germ Agglutinin","publication_year":1972,"publication_date":"1972-04-01","ids":{"openalex":"https://openalex.org/W1504713675","doi":"https://doi.org/10.1016/s0021-9258(19)45522-1","mag":"1504713675","pmid":"https://pubmed.ncbi.nlm.nih.gov/5062821"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)45522-1","doi":"https://doi.org/10.1016/s0021-9258(19)45522-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)45522-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)45522-1","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111621403","display_name":"Yoshiho Nagata","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yoshiho Nagata","raw_affiliation_strings":["From the Department of Biochemical Sciences, Moffett Laboratories, Princeton University, Princeton, New Jersey 08540"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biochemical Sciences, Moffett Laboratories, Princeton University, Princeton, New Jersey 08540","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111692165","display_name":"Max M. Burger","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Max M. Burger","raw_affiliation_strings":["From the Department of Biochemical Sciences, Moffett Laboratories, Princeton University, Princeton, New Jersey 08540"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biochemical Sciences, Moffett Laboratories, Princeton University, Princeton, New Jersey 08540","institution_ids":["https://openalex.org/I20089843"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I20089843"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":7.1917,"has_fulltext":false,"cited_by_count":112,"citation_normalized_percentile":{"value":0.98405517,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"247","issue":"7","first_page":"2248","last_page":"2250"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/wheat-germ-agglutinin","display_name":"wheat germ agglutinin","score":1.0},{"id":"https://openalex.org/keywords/lectin-purification","display_name":"lectin purification","score":0.6290000081062317},{"id":"https://openalex.org/keywords/protein-crystallization","display_name":"protein crystallization","score":0.7369999885559082},{"id":"https://openalex.org/keywords/wheat-germ-lipase","display_name":"wheat germ lipase","score":0.8740000128746033},{"id":"https://openalex.org/keywords/glycoproteins","display_name":"glycoproteins","score":0.9229999780654907},{"id":"https://openalex.org/keywords/carbohydrate-composition","display_name":"carbohydrate composition","score":0.7749999761581421},{"id":"https://openalex.org/keywords/amino-acid-composition","display_name":"amino acid composition","score":0.878000020980835},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.6359999775886536},{"id":"https://openalex.org/keywords/cystine-content","display_name":"cystine content","score":0.5550000071525574},{"id":"https://openalex.org/keywords/glycine-content","display_name":"glycine content","score":0.6869999766349792},{"id":"https://openalex.org/keywords/glucose","display_name":"glucose","score":0.6890000104904175}],"concepts":[{"id":"https://openalex.org/C2780175098","wikidata":"https://www.wikidata.org/wiki/Q2647799","display_name":"Wheat germ agglutinin","level":3,"score":0.6547244191169739},{"id":"https://openalex.org/C2780258238","wikidata":"https://www.wikidata.org/wiki/Q2520842","display_name":"Germ","level":2,"score":0.6181444525718689},{"id":"https://openalex.org/C3020307904","wikidata":"https://www.wikidata.org/wiki/Q1641345","display_name":"Wheat germ","level":2,"score":0.576210618019104},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4795379638671875},{"id":"https://openalex.org/C207545495","wikidata":"https://www.wikidata.org/wiki/Q2573513","display_name":"Agglutinin","level":3,"score":0.474448561668396},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2700173854827881},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2686046361923218},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.18661877512931824},{"id":"https://openalex.org/C2780758401","wikidata":"https://www.wikidata.org/wiki/Q408693","display_name":"Lectin","level":2,"score":0.06996852159500122}],"mesh":[{"descriptor_ui":"D000372","descriptor_name":"Agglutination Tests","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D000645","descriptor_name":"Ammonium Sulfate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002241","descriptor_name":"Carbohydrates","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D002848","descriptor_name":"Chromatography, DEAE-Cellulose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007939","descriptor_name":"Leukemia L1210","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008049","descriptor_name":"Lipase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012639","descriptor_name":"Seeds","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012967","descriptor_name":"Sodium Dodecyl Sulfate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014461","descriptor_name":"Ultracentrifugation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014908","descriptor_name":"Triticum","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D014908","descriptor_name":"Triticum","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D037102","descriptor_name":"Lectins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D037102","descriptor_name":"Lectins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D037102","descriptor_name":"Lectins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D037121","descriptor_name":"Plant Lectins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)45522-1","doi":"https://doi.org/10.1016/s0021-9258(19)45522-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)45522-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:5062821","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/5062821","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)45522-1","doi":"https://doi.org/10.1016/s0021-9258(19)45522-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)45522-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":13,"referenced_works":["https://openalex.org/W1480650704","https://openalex.org/W1544849627","https://openalex.org/W1561750927","https://openalex.org/W1590329681","https://openalex.org/W1970253549","https://openalex.org/W1987226327","https://openalex.org/W1999629737","https://openalex.org/W2012221275","https://openalex.org/W2019433339","https://openalex.org/W2020567063","https://openalex.org/W2056715324","https://openalex.org/W2082752643","https://openalex.org/W2083682303"],"related_works":["https://openalex.org/W1901786271","https://openalex.org/W1973058910","https://openalex.org/W92134007","https://openalex.org/W2045375500","https://openalex.org/W2408691890","https://openalex.org/W2389459866","https://openalex.org/W1238880271","https://openalex.org/W2407850170","https://openalex.org/W2382268723","https://openalex.org/W2045034159"],"abstract_inverted_index":{"Abstract":[0],"Procedures":[1],"for":[2,33],"the":[3,34,72],"isolation,":[4],"purification,":[5],"and":[6,41,54,65],"crystallization":[7],"of":[8,29,38],"wheat":[9,23],"germ":[10,24],"agglutinin":[11,15,48],"are":[12,57],"described.":[13],"The":[14,47,59],"was":[16,31,68],"purified":[17],"184-fold":[18],"to":[19,70],"homogeneity":[20],"from":[21],"commercial":[22],"lipase.":[25],"A":[26],"molecular":[27],"weight":[28],"23,500":[30],"estimated":[32],"protein":[35,60],"by":[36],"means":[37],"sedimentation":[39],"equilibrium":[40],"sodium":[42],"dodecyl":[43],"sulfate":[44],"gel":[45],"electrophoresis.":[46],"is":[49],"a":[50],"glycoprotein.":[51],"Amino":[52],"acid":[53],"carbohydrate":[55,74],"compositions":[56],"reported.":[58],"contains":[61],"unusually":[62],"high":[63],"half-cystine":[64],"glycine.":[66],"Glucose":[67],"found":[69],"be":[71],"major":[73],"constituent.":[75]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}