{"id":"https://openalex.org/W1504459888","doi":null,"title":"Identification and characterization of enzymes involved in biosynthesis of different phycobiliproteins in cyanobacteria","display_name":"Identification and characterization of enzymes involved in biosynthesis of different phycobiliproteins in cyanobacteria","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W1504459888","mag":"1504459888"},"language":"en","primary_location":{"id":"pmh:oai:scholarworks.uno.edu:td-1244","doi":null,"is_oa":false,"landing_page_url":"https://scholarworks.uno.edu/td/446","pdf_url":null,"source":{"id":"https://openalex.org/S4306401789","display_name":"ScholarWorks @ The University of New Orleans (The University of New Orleans)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I192396691","host_organization_name":"LSU New Orleans","host_organization_lineage":["https://openalex.org/I192396691"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"University of New Orleans Theses and Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102131439","display_name":"Avijit Biswas","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Avijit Biswas","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5102131439"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.107,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.43016156,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.8682000041007996,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.8682000041007996,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.035999998450279236,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12236","display_name":"Photoreceptor and optogenetics research","score":0.027499999850988388,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phycobiliproteins","display_name":"phycobiliproteins","score":0.9539999961853027},{"id":"https://openalex.org/keywords/cyanobacteria","display_name":"cyanobacteria","score":0.8809999823570251},{"id":"https://openalex.org/keywords/allophycocyanin","display_name":"allophycocyanin","score":0.9359999895095825},{"id":"https://openalex.org/keywords/chromophore-attachment","display_name":"chromophore attachment","score":0.6579999923706055},{"id":"https://openalex.org/keywords/peb","display_name":"PEB","score":0.33899998664855957},{"id":"https://openalex.org/keywords/pcbs","display_name":"PCBs","score":0.8560000061988831},{"id":"https://openalex.org/keywords/heterologous-expression-in-escherichia-coli","display_name":"heterologous expression in Escherichia coli","score":0.6990000009536743},{"id":"https://openalex.org/keywords/synechococcus-sp-pcc-7002","display_name":"Synechococcus sp. PCC 7002","score":0.9449999928474426},{"id":"https://openalex.org/keywords/synechocystis-sp-pcc-6803","display_name":"Synechocystis sp. PCC 6803","score":0.9929999709129333},{"id":"https://openalex.org/keywords/fremyella-diplosiphon","display_name":"Fremyella diplosiphon","score":0.8190000057220459},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.7200000286102295}],"concepts":[{"id":"https://openalex.org/C116514494","wikidata":"https://www.wikidata.org/wiki/Q3234015","display_name":"Phycobiliprotein","level":4,"score":0.911152720451355},{"id":"https://openalex.org/C2779669040","wikidata":"https://www.wikidata.org/wiki/Q93315","display_name":"Cyanobacteria","level":3,"score":0.8779368996620178},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.6243071556091309},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.6022801995277405},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5156675577163696},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.46304017305374146},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37886568903923035},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36307042837142944},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.33409640192985535},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.17787137627601624},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1686060130596161},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09191909432411194}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:scholarworks.uno.edu:td-1244","doi":null,"is_oa":false,"landing_page_url":"https://scholarworks.uno.edu/td/446","pdf_url":null,"source":{"id":"https://openalex.org/S4306401789","display_name":"ScholarWorks @ The University of New Orleans (The University of New Orleans)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I192396691","host_organization_name":"LSU New Orleans","host_organization_lineage":["https://openalex.org/I192396691"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"University of New Orleans Theses and Dissertations","raw_type":"text"},{"id":"mag:1504459888","doi":null,"is_oa":false,"landing_page_url":"https://scholarworks.uno.edu/cgi/viewcontent.cgi?article=1244&context=td","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":156,"referenced_works":["https://openalex.org/W95925309","https://openalex.org/W100074522","https://openalex.org/W173620192","https://openalex.org/W236354049","https://openalex.org/W562908385","https://openalex.org/W566083668","https://openalex.org/W584295467","https://openalex.org/W601072063","https://openalex.org/W1483098528","https://openalex.org/W1499112972","https://openalex.org/W1501220712","https://openalex.org/W1501566966","https://openalex.org/W1503676980","https://openalex.org/W1506423406","https://openalex.org/W1537538267","https://openalex.org/W1542263802","https://openalex.org/W1542493114","https://openalex.org/W1558990596","https://openalex.org/W1571461926","https://openalex.org/W1573964975","https://openalex.org/W1575497848","https://openalex.org/W1587723494","https://openalex.org/W1587782352","https://openalex.org/W1589288705","https://openalex.org/W1596823808","https://openalex.org/W1626592198","https://openalex.org/W1651995608","https://openalex.org/W1730564863","https://openalex.org/W1834586755","https://openalex.org/W1921439897","https://openalex.org/W1942589607","https://openalex.org/W1966619274","https://openalex.org/W1971701354","https://openalex.org/W1972125146","https://openalex.org/W1977286193","https://openalex.org/W1979470755","https://openalex.org/W1980219352","https://openalex.org/W1980703707","https://openalex.org/W1984073014","https://openalex.org/W1984455134","https://openalex.org/W1988576580","https://openalex.org/W1989499375","https://openalex.org/W1990441231","https://openalex.org/W1991668255","https://openalex.org/W1993501991","https://openalex.org/W1997389714","https://openalex.org/W2000869729","https://openalex.org/W2002365254","https://openalex.org/W2003399853","https://openalex.org/W2004508099","https://openalex.org/W2004800185","https://openalex.org/W2005553703","https://openalex.org/W2009229612","https://openalex.org/W2009471785","https://openalex.org/W2010579441","https://openalex.org/W2014537275","https://openalex.org/W2015717581","https://openalex.org/W2016285887","https://openalex.org/W2016405867","https://openalex.org/W2018214493","https://openalex.org/W2020969248","https://openalex.org/W2024488250","https://openalex.org/W2026527900","https://openalex.org/W2028674389","https://openalex.org/W2029530935","https://openalex.org/W2030819630","https://openalex.org/W2031533876","https://openalex.org/W2035548607","https://openalex.org/W2036742051","https://openalex.org/W2036815314","https://openalex.org/W2038756774","https://openalex.org/W2040141270","https://openalex.org/W2040497188","https://openalex.org/W2042939696","https://openalex.org/W2043984223","https://openalex.org/W2044670036","https://openalex.org/W2044775952","https://openalex.org/W2045358843","https://openalex.org/W2045541819","https://openalex.org/W2049586588","https://openalex.org/W2050645062","https://openalex.org/W2050846271","https://openalex.org/W2051290357","https://openalex.org/W2051364846","https://openalex.org/W2052512546","https://openalex.org/W2052795878","https://openalex.org/W2054828157","https://openalex.org/W2055386590","https://openalex.org/W2056644087","https://openalex.org/W2056794477","https://openalex.org/W2058029566","https://openalex.org/W2058054302","https://openalex.org/W2058254034","https://openalex.org/W2059614556","https://openalex.org/W2059884782","https://openalex.org/W2061104342","https://openalex.org/W2062584177","https://openalex.org/W2062676118","https://openalex.org/W2063302275","https://openalex.org/W2065859138","https://openalex.org/W2066243382","https://openalex.org/W2066383202","https://openalex.org/W2070587063","https://openalex.org/W2070906205","https://openalex.org/W2071432034","https://openalex.org/W2072593386","https://openalex.org/W2074132949","https://openalex.org/W2077081813","https://openalex.org/W2079601505","https://openalex.org/W2081636630","https://openalex.org/W2082433067","https://openalex.org/W2083165115","https://openalex.org/W2084312697","https://openalex.org/W2084639413","https://openalex.org/W2087165938","https://openalex.org/W2089737984","https://openalex.org/W2093057466","https://openalex.org/W2095034518","https://openalex.org/W2100023269","https://openalex.org/W2101136171","https://openalex.org/W2102214793","https://openalex.org/W2102557954","https://openalex.org/W2112920640","https://openalex.org/W2116045875","https://openalex.org/W2117749312","https://openalex.org/W2118540866","https://openalex.org/W2119612945","https://openalex.org/W2125415458","https://openalex.org/W2125860910","https://openalex.org/W2128031791","https://openalex.org/W2128785980","https://openalex.org/W2130671018","https://openalex.org/W2132688566","https://openalex.org/W2139637333","https://openalex.org/W2140470175","https://openalex.org/W2142180132","https://openalex.org/W2143444184","https://openalex.org/W2145852201","https://openalex.org/W2147587362","https://openalex.org/W2148566212","https://openalex.org/W2155311371","https://openalex.org/W2160148210","https://openalex.org/W2160430130","https://openalex.org/W2161458381","https://openalex.org/W2161996750","https://openalex.org/W2168795815","https://openalex.org/W2169093137","https://openalex.org/W2169460995","https://openalex.org/W2172262258","https://openalex.org/W2182166571","https://openalex.org/W2256163163","https://openalex.org/W2465788881","https://openalex.org/W2487532034","https://openalex.org/W2952826562","https://openalex.org/W3022311348","https://openalex.org/W3206063407"],"related_works":["https://openalex.org/W2145852201","https://openalex.org/W2278058891","https://openalex.org/W2626687514","https://openalex.org/W2127397350","https://openalex.org/W1979470755","https://openalex.org/W1536784812","https://openalex.org/W2030819630","https://openalex.org/W2059614556","https://openalex.org/W6921997373","https://openalex.org/W2100023269"],"abstract_inverted_index":{"A":[0],"multi-plasmid,":[1],"co-expression":[2,127],"system":[3,24,34,80,128],"was":[4,35,64,81,144,156,215,242,254,314],"used":[5,36,82],"to":[6,37,49,71,83,158,172,229,244,283,289],"recreate":[7],"the":[8,14,97,105,132,147,207,317],"biosynthetic":[9],"pathway":[10],"for":[11,113,303],"phycobiliproteins":[12],"from":[13,86,152,235,309],"cyanobacterium":[15],"Synechococcus":[16,310],"sp.":[17,88,311],"PCC":[18,89],"7002":[19],"in":[20,66,118,129,239,259,268],"E.":[21,130,318],"coli.":[22],"This":[23,33],"efficiently":[25,226],"produced":[26,65],"chromophorylated":[27,84],"allophycocyanin":[28,50],"(ApcA/ApcB),":[29],"\uf061-phycocyanin,":[30],"and":[31,41,54,69,142,149,182,202,273],"\uf062-phycocyanin.":[32],"demonstrate":[38],"that":[39,185],"CpcS-I":[40],"CpcU":[42],"proteins":[43],"are":[44],"both":[45,200,271],"required":[46],"attaching":[47],"PCB":[48,114,246],"subunits":[51,139,151,275],"ApcD":[52],"(\uf061AP-B)":[53],"ApcF":[55],"(\uf06218).":[56],"The":[57,193],"N-terminal,":[58],"AP-like":[59],"domain":[60],"of":[61,99,136,179,204,209,231,251,270,276,299],"ApcE":[62],"(LCM99)":[63],"soluble":[67],"form":[68],"shown":[70],"have":[72,245],"intrinsic":[73],"bilin":[74,102,117,195,300,326],"lyase":[75,108,138,196,301],"activity.":[76],"In":[77],"addition,":[78],"this":[79],"CpcA":[85],"Synechococystis":[87],"6803":[90],"with":[91,96,176,220,278,331],"a":[92,125,262,279,296],"non-cognate":[93],"bilin;":[94],"PEB":[95,133,160,188,198,211,228,291],"aid":[98],"CpcEF":[100,258],"type":[101,107,285,298],"lyase.":[103],"However,":[104,224,261],"CpcSU":[106],"displays":[109],"much":[110,216],"higher":[111],"specificity":[112],"(the":[115],"native":[116],"these":[119],"species)":[120],"than":[121,218],"PEB.":[122],"Next,":[123],"using":[124,316],"heterologous,":[126],"coli,":[131],"ligation":[134],"activity":[135,170],"putative":[137],"CpeY,":[140],"CpeZ,":[141],"CpeS":[143,194,225],"tested":[145],"on":[146,161,191,248,292,333],"CpeA":[148,180],"CpeB":[150],"F.":[153,240],"diplosiphon.":[154],"CpeY/CpeZ":[155,186],"found":[157,243],"ligate":[159],"CpeA,":[162,205],"although":[163],"CpeY":[164,221],"alone":[165],"had":[166],"only":[167],"60%":[168],"chromophorylation":[169,269],"compared":[171,282],"CpeYZ":[173],"together.":[174],"Studies":[175],"site-directed":[177],"variants":[178],"(C82S":[181],"C139S),":[183],"revealed":[184],"attached":[187,210,227],"at":[189,199,212],"Cys-82":[190,201,230],"HT-CpeA.":[192],"ligated":[197],"Cys-139":[203],"but":[206],"yield":[208],"Cys":[213,334],"82":[214],"lower":[217],"observed":[219],"or":[222],"CpeY/CpeZ.":[223],"CpeB.":[232],"Purified":[233],"PE":[234,277,286,293],"cpeY":[236],"deletion":[237],"mutants":[238],"diplosiphon":[241],"added":[247],"\u03b1-PE":[249],"instead":[250],"PEB,":[252],"which":[253],"likely":[255],"performed":[256],"by":[257],"vivo.":[260],"cpeZ":[263],"knock-out":[264],"mutant":[265],"is":[266],"affected":[267],"\uf061":[272],"\uf062":[274],"red-shifted":[280],"absorbance":[281],"wild":[284],"probably":[287],"due":[288],"missing":[290],"subunits.":[294],"Next":[295],"new":[297],"isomerase":[302],"PEII":[304],"(\uf061":[305],"subunit)":[306],"named":[307],"MpeZ":[308,323],"RS":[312],"9916,":[313],"analyzed":[315],"coli":[319],"heterologous":[320],"coexpression":[321],"system.":[322],"acted":[324],"as":[325],"lyase/isomerase":[327],"chromophorylating":[328],"\u03b1-PEII":[329],"(MpeA)":[330],"PUB":[332],"83.":[335]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-01T08:54:57.152042","created_date":"2016-06-24T00:00:00"}
