{"id":"https://openalex.org/W1504374540","doi":"https://doi.org/10.1016/s0076-6879(09)04614-x","title":"Chapter 14 Design and Synthesis of Pathway Genes for Polyketide Biosynthesis","display_name":"Chapter 14 Design and Synthesis of Pathway Genes for Polyketide Biosynthesis","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W1504374540","doi":"https://doi.org/10.1016/s0076-6879(09)04614-x","mag":"1504374540","pmid":"https://pubmed.ncbi.nlm.nih.gov/19362646"},"language":"en","primary_location":{"id":"doi:10.1016/s0076-6879(09)04614-x","doi":"https://doi.org/10.1016/s0076-6879(09)04614-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0076-6879(09)04614-x","pdf_url":"https://www.sciencedirect.com/science/article/pii/S007668790904614X","source":{"id":"https://openalex.org/S182211142","display_name":"Methods in enzymology on CD-ROM/Methods in enzymology","issn_l":"0076-6879","issn":["0076-6879","1079-2376","1557-7988"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319955","host_organization_name":"Academic Press","host_organization_lineage":["https://openalex.org/P4310319955","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Academic Press","Elsevier BV"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Enzymology","raw_type":"book-chapter"},"volume":null,"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.sciencedirect.com/science/article/pii/S007668790904614X","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026105193","display_name":"Salvador Peir\u00fa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]},{"id":"https://openalex.org/I4210147873","display_name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario","ror":"https://ror.org/04x0n3178","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210107757","https://openalex.org/I4210123736","https://openalex.org/I4210147873","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Salvador Peir\u00fa","raw_affiliation_strings":["Instituto de Biolog\u00eda Molecular y Celular de Rosario (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Biolog\u00eda Molecular y Celular de Rosario (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210147873"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000309210","display_name":"Hugo C. Gramajo","orcid":"https://orcid.org/0000-0003-3384-2979","observed_orcids":["https://orcid.org/0000-0003-3384-2979"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]},{"id":"https://openalex.org/I4210147873","display_name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario","ror":"https://ror.org/04x0n3178","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210107757","https://openalex.org/I4210123736","https://openalex.org/I4210147873","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Hugo Gramajo","raw_affiliation_strings":["Instituto de Biolog\u00eda Molecular y Celular de Rosario (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Biolog\u00eda Molecular y Celular de Rosario (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210147873"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5020401119","display_name":"Hugo G. Menzella","orcid":"https://orcid.org/0000-0001-6930-2400","observed_orcids":["https://orcid.org/0000-0001-6930-2400"]},"institutions":[{"id":"https://openalex.org/I151201029","display_name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","ror":"https://ror.org/03cqe8w59","country_code":"AR","type":"government","lineage":["https://openalex.org/I151201029","https://openalex.org/I4210123736","https://openalex.org/I4387155568"]},{"id":"https://openalex.org/I4141589","display_name":"National University of Rosario","ror":"https://ror.org/02tphfq59","country_code":"AR","type":"education","lineage":["https://openalex.org/I4141589"]},{"id":"https://openalex.org/I4210147873","display_name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario","ror":"https://ror.org/04x0n3178","country_code":"AR","type":"facility","lineage":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210107757","https://openalex.org/I4210123736","https://openalex.org/I4210147873","https://openalex.org/I4387155568"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Hugo G. Menzella","raw_affiliation_strings":["Instituto de Biolog\u00eda Molecular y Celular de Rosario (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto de Biolog\u00eda Molecular y Celular de Rosario (CONICET), Facultad de Ciencias Bioqu\u00edmicas y Farmac\u00e9uticas, Universidad Nacional de Rosario, Rosario, Argentina","institution_ids":["https://openalex.org/I151201029","https://openalex.org/I4141589","https://openalex.org/I4210147873"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.9376,"has_fulltext":true,"cited_by_count":6,"citation_normalized_percentile":{"value":0.86413043,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"459","issue":null,"first_page":"319","last_page":"337"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/polyketide-biosynthesis","display_name":"polyketide biosynthesis","score":0.9210000038146973},{"id":"https://openalex.org/keywords/type-i-polyketide-synthase","display_name":"type I polyketide synthase","score":0.9679999947547913},{"id":"https://openalex.org/keywords/synthetic-gene-design","display_name":"synthetic gene design","score":0.8610000014305115},{"id":"https://openalex.org/keywords/chimeric-enzymes","display_name":"chimeric enzymes","score":0.722000002861023},{"id":"https://openalex.org/keywords/codon-optimization","display_name":"codon optimization","score":0.6629999876022339},{"id":"https://openalex.org/keywords/desosamine","display_name":"desosamine","score":0.9990000128746033},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.9129999876022339}],"concepts":[{"id":"https://openalex.org/C2777763344","wikidata":"https://www.wikidata.org/wiki/Q4369963","display_name":"Polyketide synthase","level":5,"score":0.8237706422805786},{"id":"https://openalex.org/C2778635478","wikidata":"https://www.wikidata.org/wiki/Q516751","display_name":"Polyketide","level":4,"score":0.6516196727752686},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6474334597587585},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5635752081871033},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.5548186302185059},{"id":"https://openalex.org/C203075996","wikidata":"https://www.wikidata.org/wiki/Q139677","display_name":"Operon","level":4,"score":0.5340805053710938},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.4933904707431793},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.488104909658432},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4551614820957184},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.41529595851898193},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.3798547685146332}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005818","descriptor_name":"Genetic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009876","descriptor_name":"Operon","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018942","descriptor_name":"Macrolides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D048630","descriptor_name":"Polyketide Synthases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0076-6879(09)04614-x","doi":"https://doi.org/10.1016/s0076-6879(09)04614-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0076-6879(09)04614-x","pdf_url":"https://www.sciencedirect.com/science/article/pii/S007668790904614X","source":{"id":"https://openalex.org/S182211142","display_name":"Methods in enzymology on CD-ROM/Methods in enzymology","issn_l":"0076-6879","issn":["0076-6879","1079-2376","1557-7988"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319955","host_organization_name":"Academic Press","host_organization_lineage":["https://openalex.org/P4310319955","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Academic Press","Elsevier BV"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Enzymology","raw_type":"book-chapter"},{"id":"pmid:19362646","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19362646","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in enzymology","raw_type":"Journal Article"},{"id":"pmh:oai:ri.conicet.gov.ar:11336/139745","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11336/139745","pdf_url":null,"source":{"id":"https://openalex.org/S4306402455","display_name":"CONICET Digital (CONICET)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210148385","host_organization_name":"Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society","host_organization_lineage":["https://openalex.org/I4210148385"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1016/s0076-6879(09)04614-x","doi":"https://doi.org/10.1016/s0076-6879(09)04614-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0076-6879(09)04614-x","pdf_url":"https://www.sciencedirect.com/science/article/pii/S007668790904614X","source":{"id":"https://openalex.org/S182211142","display_name":"Methods in enzymology on CD-ROM/Methods in enzymology","issn_l":"0076-6879","issn":["0076-6879","1079-2376","1557-7988"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319955","host_organization_name":"Academic Press","host_organization_lineage":["https://openalex.org/P4310319955","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Academic Press","Elsevier BV"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Enzymology","raw_type":"book-chapter"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1504374540.pdf","grobid_xml":"https://content.openalex.org/works/W1504374540.grobid-xml"},"referenced_works_count":31,"referenced_works":["https://openalex.org/W1494581922","https://openalex.org/W1968418450","https://openalex.org/W1968839115","https://openalex.org/W1971809625","https://openalex.org/W1981740353","https://openalex.org/W1991170879","https://openalex.org/W1992837075","https://openalex.org/W1998307795","https://openalex.org/W2001396254","https://openalex.org/W2003706451","https://openalex.org/W2006677219","https://openalex.org/W2013302570","https://openalex.org/W2017621033","https://openalex.org/W2024672561","https://openalex.org/W2034756351","https://openalex.org/W2035522315","https://openalex.org/W2035673487","https://openalex.org/W2059719012","https://openalex.org/W2063922582","https://openalex.org/W2068275245","https://openalex.org/W2072302714","https://openalex.org/W2073091301","https://openalex.org/W2074137186","https://openalex.org/W2077589208","https://openalex.org/W2077896560","https://openalex.org/W2081228145","https://openalex.org/W2088524774","https://openalex.org/W2094409918","https://openalex.org/W2125281765","https://openalex.org/W2132042949","https://openalex.org/W2166281636"],"related_works":["https://openalex.org/W1981740353","https://openalex.org/W1992837075","https://openalex.org/W2035522315","https://openalex.org/W3023834571","https://openalex.org/W4409565457","https://openalex.org/W6944092926","https://openalex.org/W2398193949","https://openalex.org/W2810949692","https://openalex.org/W4401220553","https://openalex.org/W2787778190"],"abstract_inverted_index":null,"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}