{"id":"https://openalex.org/W1504209205","doi":null,"title":"Decreasing expression of the G1-phase inhibitors, p21Cip1 and p16INK4a, promotes division of corneal endothelial cells from older donors.","display_name":"Decreasing expression of the G1-phase inhibitors, p21Cip1 and p16INK4a, promotes division of corneal endothelial cells from older donors.","publication_year":2010,"publication_date":"2010-05-25","ids":{"openalex":"https://openalex.org/W1504209205","mag":"1504209205","pmid":"https://pubmed.ncbi.nlm.nih.gov/20508865","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2875254"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2875254","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2875254","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Vis","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2875254","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083436687","display_name":"Nancy C. Joyce","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nancy C Joyce","raw_affiliation_strings":["Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA. nancy.joyce@schepens.harvard.edu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA. nancy.joyce@schepens.harvard.edu","institution_ids":["https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5029025730","display_name":"Deshea L Harris","orcid":"https://orcid.org/0000-0002-8194-1827","observed_orcids":["https://orcid.org/0000-0002-8194-1827"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Deshea L Harris","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":7.4036,"has_fulltext":false,"cited_by_count":51,"citation_normalized_percentile":{"value":0.97146493,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"16","issue":null,"first_page":"897","last_page":"906"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11828","display_name":"Corneal Surgery and Treatments","score":0.7407000064849854,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11828","display_name":"Corneal Surgery and Treatments","score":0.7407000064849854,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.10920000076293945,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10563","display_name":"Corneal surgery and disorders","score":0.032999999821186066,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/p21","display_name":"p21","score":0.9150000214576721},{"id":"https://openalex.org/keywords/p16ink4a","display_name":"p16INK4a","score":1.0},{"id":"https://openalex.org/keywords/corneal-endothelial-cells","display_name":"corneal endothelial cells","score":0.9020000100135803},{"id":"https://openalex.org/keywords/g1-cell-cycle-arrest","display_name":"G1 cell cycle arrest","score":0.824999988079071},{"id":"https://openalex.org/keywords/cyclin-dependent-kinase-inhibitors","display_name":"cyclin-dependent kinase inhibitors","score":0.9950000047683716},{"id":"https://openalex.org/keywords/sirna-knockdown","display_name":"siRNA knockdown","score":0.7730000019073486},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.8360000252723694},{"id":"https://openalex.org/keywords/cell-cycle-entry","display_name":"cell cycle entry","score":0.5529999732971191},{"id":"https://openalex.org/keywords/corneal-endothelial-cell-density","display_name":"corneal endothelial cell density","score":0.7049999833106995},{"id":"https://openalex.org/keywords/ki-67","display_name":"Ki-67","score":0.7160000205039978}],"concepts":[{"id":"https://openalex.org/C186852380","wikidata":"https://www.wikidata.org/wiki/Q1142521","display_name":"Electroporation","level":3,"score":0.6694068312644958},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.650800883769989},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5937463641166687},{"id":"https://openalex.org/C22615655","wikidata":"https://www.wikidata.org/wiki/Q203221","display_name":"Small interfering RNA","level":4,"score":0.5827046036720276},{"id":"https://openalex.org/C2778437423","wikidata":"https://www.wikidata.org/wiki/Q5198265","display_name":"Cyclin B1","level":5,"score":0.5458403825759888},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5450568199157715},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.5221993327140808},{"id":"https://openalex.org/C2777527632","wikidata":"https://www.wikidata.org/wiki/Q238382","display_name":"DAPI","level":3,"score":0.5110162496566772},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.4852147400379181},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.42471277713775635},{"id":"https://openalex.org/C120089663","wikidata":"https://www.wikidata.org/wiki/Q59250999","display_name":"Cyclin","level":4,"score":0.41950303316116333},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.41583192348480225},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.3608103394508362},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.34293270111083984},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.2751399874687195},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.21598714590072632},{"id":"https://openalex.org/C120504264","wikidata":"https://www.wikidata.org/wiki/Q1147372","display_name":"Cyclin-dependent kinase 1","level":4,"score":0.2078518271446228},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1004919707775116},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07011950016021729},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000375","descriptor_name":"Aging","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002453","descriptor_name":"Cell Cycle","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004728","descriptor_name":"Endothelium, Corneal","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015536","descriptor_name":"Down-Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016193","descriptor_name":"G1 Phase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018274","descriptor_name":"Electroporation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019941","descriptor_name":"Cyclin-Dependent Kinase Inhibitor p16","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019941","descriptor_name":"Cyclin-Dependent Kinase Inhibitor p16","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D042783","descriptor_name":"Endothelial Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D042783","descriptor_name":"Endothelial Cells","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D050759","descriptor_name":"Cyclin-Dependent Kinase Inhibitor p21","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D050759","descriptor_name":"Cyclin-Dependent Kinase Inhibitor p21","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":3,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:2875254","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2875254","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Vis","raw_type":"Text"},{"id":"pmid:20508865","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20508865","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular vision","raw_type":"Journal Article"},{"id":"mag:1504209205","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20508865","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2875254","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2875254","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mol Vis","raw_type":"Text"},"sustainable_development_goals":[{"score":0.98580002784729,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4785950277","display_name":"Molecular Induction of Corneal Endothelial Proliferation","funder_award_id":"R01EY012700","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W69962339","https://openalex.org/W116134068","https://openalex.org/W133348346","https://openalex.org/W134095429","https://openalex.org/W1494254537","https://openalex.org/W1501914448","https://openalex.org/W1523805938","https://openalex.org/W1558909828","https://openalex.org/W1965941046","https://openalex.org/W1975324295","https://openalex.org/W1982870374","https://openalex.org/W1988354512","https://openalex.org/W1990086621","https://openalex.org/W1992934869","https://openalex.org/W1996413533","https://openalex.org/W1997505481","https://openalex.org/W2001627329","https://openalex.org/W2002821854","https://openalex.org/W2010493387","https://openalex.org/W2015519364","https://openalex.org/W2040843131","https://openalex.org/W2041573061","https://openalex.org/W2044057392","https://openalex.org/W2049075369","https://openalex.org/W2063786789","https://openalex.org/W2070829404","https://openalex.org/W2072088194","https://openalex.org/W2086269682","https://openalex.org/W2089218510","https://openalex.org/W2119651508","https://openalex.org/W2120162119","https://openalex.org/W2121739926","https://openalex.org/W2134414575"],"related_works":["https://openalex.org/W1982870374","https://openalex.org/W2040843131","https://openalex.org/W2078063257","https://openalex.org/W2529949335","https://openalex.org/W7095923826","https://openalex.org/W2088246277","https://openalex.org/W1570369748","https://openalex.org/W1500260818","https://openalex.org/W120471618","https://openalex.org/W7097052143"],"abstract_inverted_index":{"PURPOSE:":[0],"The":[1,96,122,144,183,259],"current":[2],"studies":[3],"were":[4,66,82,201],"conducted":[5],"to":[6,78,127,177,256,299,304],"determine":[7],"whether":[8],"the":[9,38,47,85,129,180,205,215,225,245,268,273,285,295],"cyclin-dependent":[10,15,22],"kinase":[11,23],"inhibitors,":[12],"p21Cip1":[13,133,198,236,288],"(p21":[14],"kinase-interacting":[16],"protein":[17,130],"1)":[18],"and":[19,56,75,119,134,153,159,168,199,223,237,289],"p16INK4a":[20,135,200,238,290],"(p16":[21],"inhibitor":[24],"1A),":[25],"help":[26],"mediate":[27],"G(1)-phase":[28],"inhibition":[29],"in":[30,58,84,230,241,248,262,294],"human":[31],"corneal":[32,306],"endothelial":[33,307],"cells":[34,117,167,220,229,271],"(HCEC)":[35],"by":[36,103,113,138,164,179],"testing":[37],"effect":[39,145],"of":[40,46,49,87,98,106,116,124,132,141,146,196,207,218,228,244,264,270,284,287,297],"siRNA":[41,91,99,126,147,173,209],"(small":[42],"interfering":[43],"RNA)-mediated":[44],"down-regulation":[45],"expression":[48,131,263],"these":[50,265],"inhibitors":[51],"on":[52,149],"cell":[53,150,185,246,257,274,280,302,308],"cycle":[54,151,247],"entry":[55],"proliferation":[57,154],"HCEC":[59,65,178,249],"cultured":[60,76],"from":[61,68],"older":[62],"donors.":[63],"METHODS:":[64],"obtained":[67],"National":[69],"Disease":[70],"Research":[71],"Interchange,":[72],"Philadelphia,":[73],"PA,":[74],"according":[77],"published":[79],"methods.":[80],"Cells":[81],"electroporated":[83],"presence":[86],"either":[88],"a":[89,231],"non-silencing":[90],"control":[92,108],"or":[93],"p21+p16":[94,125,208],"siRNA.":[95,109],"efficiency":[97],"transfer":[100],"was":[101,111,136,155,174,187],"observed":[102],"fluorescence":[104],"microscopy":[105],"Cy3-labeled":[107],"Viability":[110],"determined":[112,137,156],"direct":[114],"counting":[115,165],"before":[118],"after":[120,162],"electroporation.":[121,193],"ability":[123],"decrease":[128],"semi-quantitative":[139],"analysis":[140],"western":[142],"blots.":[143],"treatment":[148,212],"progression":[152],"1,":[157],"5,":[158],"11":[160],"days":[161],"electroporation":[163,181],"Ki67-positive":[166,219],"total":[169,226,279],"DAPI-stained":[170],"nuclei.":[171],"RESULTS:":[172],"efficiently":[175],"transferred":[176],"method.":[182],"average":[184,216],"loss":[186],"41.25%":[188],"at":[189],"24":[190],"h":[191],"following":[192],"Protein":[194],"levels":[195,286],"both":[197],"significantly":[202,213],"decreased":[203],"as":[204,276,278],"result":[206],"treatment.":[210],"This":[211],"increased":[214,224,267],"number":[217,227,269],"over":[221],"controls":[222],"time-dependent":[232],"manner.":[233],"CONCLUSIONS:":[234],"Both":[235],"are":[239],"involved":[240],"negative":[242],"regulation":[243],"and,":[250],"thereby,":[251],"provide":[252],"an":[253],"effective":[254],"barrier":[255],"division.":[258],"siRNA-induced":[260],"reduction":[261,283],"proteins":[266],"entering":[272],"cycle,":[275],"well":[277],"numbers.":[281],"Thus,":[282],"could":[291],"be":[292],"useful":[293],"development":[296],"treatments":[298],"induce":[300],"transient":[301],"division":[303],"increase":[305],"density.":[309]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":7}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
