{"id":"https://openalex.org/W1504145802","doi":"https://doi.org/10.1016/s0021-9258(19)36871-1","title":"Metal binding properties of recombinant rat parvalbumin wild-type and F102W mutant.","display_name":"Metal binding properties of recombinant rat parvalbumin wild-type and F102W mutant.","publication_year":1993,"publication_date":"1993-10-01","ids":{"openalex":"https://openalex.org/W1504145802","doi":"https://doi.org/10.1016/s0021-9258(19)36871-1","mag":"1504145802","pmid":"https://pubmed.ncbi.nlm.nih.gov/8407923"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)36871-1","doi":"https://doi.org/10.1016/s0021-9258(19)36871-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)36871-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)36871-1","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001659423","display_name":"Thomas L. Pauls","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"T.L. Pauls","raw_affiliation_strings":["Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046923893","display_name":"Isabelle Durussel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"I Durussel","raw_affiliation_strings":["Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029106499","display_name":"J A Cox","orcid":"https://orcid.org/0000-0001-8785-8947","observed_orcids":["https://orcid.org/0000-0001-8785-8947"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"J.A. Cox","raw_affiliation_strings":["Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061997223","display_name":"Ian D. Clark","orcid":"https://orcid.org/0000-0003-2927-1368","observed_orcids":["https://orcid.org/0000-0003-2927-1368"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"I.D. Clark","raw_affiliation_strings":["Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111623908","display_name":"Arthur G. Szabo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"A.G. Szabo","raw_affiliation_strings":["Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020357217","display_name":"St\u00e9phane M. Gagn\u00e9","orcid":"https://orcid.org/0000-0003-3476-2620","observed_orcids":["https://orcid.org/0000-0003-3476-2620"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"S.M. Gagn\u00e9","raw_affiliation_strings":["Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067477469","display_name":"Brian D. Sykes","orcid":"https://orcid.org/0000-0003-2544-2246","observed_orcids":["https://orcid.org/0000-0003-2544-2246"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"B.D. Sykes","raw_affiliation_strings":["Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5078423327","display_name":"Martin Werner Berchtold","orcid":"https://orcid.org/0000-0001-8995-9047","observed_orcids":["https://orcid.org/0000-0001-8995-9047"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"M.W. Berchtold","raw_affiliation_strings":["Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Veterinary Biochemistry, University of Z\u00fcrich-Irchel, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I202697423"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.3069,"has_fulltext":false,"cited_by_count":52,"citation_normalized_percentile":{"value":0.78969063,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"268","issue":"28","first_page":"20897","last_page":"20903"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.7968999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.7968999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12030","display_name":"S100 Proteins and Annexins","score":0.12700000405311584,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.060100000351667404,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/parvalbumin","display_name":"parvalbumin","score":0.9319999814033508},{"id":"https://openalex.org/keywords/ef-hand-proteins","display_name":"EF-hand proteins","score":0.7900000214576721},{"id":"https://openalex.org/keywords/calcium-binding","display_name":"calcium binding","score":0.7599999904632568},{"id":"https://openalex.org/keywords/magnesium-binding","display_name":"magnesium binding","score":1.0},{"id":"https://openalex.org/keywords/electrostatic-environment","display_name":"electrostatic environment","score":0.777999997138977},{"id":"https://openalex.org/keywords/hydrophobic-core","display_name":"hydrophobic core","score":0.7770000100135803},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8460000157356262}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.8153814077377319},{"id":"https://openalex.org/C141547260","wikidata":"https://www.wikidata.org/wiki/Q2720322","display_name":"Parvalbumin","level":2,"score":0.7660199403762817},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.7472070455551147},{"id":"https://openalex.org/C544153396","wikidata":"https://www.wikidata.org/wiki/Q11426","display_name":"Metal","level":2,"score":0.49133315682411194},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4910602569580078},{"id":"https://openalex.org/C207583985","wikidata":"https://www.wikidata.org/wiki/Q128723","display_name":"Wild type","level":4,"score":0.47342756390571594},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.38549238443374634},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3784494400024414},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3494676947593689},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.26912015676498413},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.20900008082389832},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008274","descriptor_name":"Magnesium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008670","descriptor_name":"Metals","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009838","descriptor_name":"Oligodeoxyribonucleotides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010320","descriptor_name":"Parvalbumins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010320","descriptor_name":"Parvalbumins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010320","descriptor_name":"Parvalbumins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013056","descriptor_name":"Spectrophotometry, Ultraviolet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)36871-1","doi":"https://doi.org/10.1016/s0021-9258(19)36871-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)36871-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:8407923","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8407923","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)36871-1","doi":"https://doi.org/10.1016/s0021-9258(19)36871-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)36871-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W1160515743","https://openalex.org/W1422702274","https://openalex.org/W1503782530","https://openalex.org/W1513843435","https://openalex.org/W1555210713","https://openalex.org/W1587148368","https://openalex.org/W1596808809","https://openalex.org/W1660937480","https://openalex.org/W1964559556","https://openalex.org/W1970592468","https://openalex.org/W1984098146","https://openalex.org/W1984488163","https://openalex.org/W1985367264","https://openalex.org/W1988464047","https://openalex.org/W1989374609","https://openalex.org/W1991622845","https://openalex.org/W1998493256","https://openalex.org/W2011567183","https://openalex.org/W2015501698","https://openalex.org/W2029625486","https://openalex.org/W2034077172","https://openalex.org/W2038412379","https://openalex.org/W2039366113","https://openalex.org/W2044961004","https://openalex.org/W2045788459","https://openalex.org/W2061734875","https://openalex.org/W2072834895","https://openalex.org/W2083780170","https://openalex.org/W2111530979","https://openalex.org/W2138270253","https://openalex.org/W2204636683","https://openalex.org/W2235129190","https://openalex.org/W2429942626","https://openalex.org/W2707885902","https://openalex.org/W2888511473"],"related_works":["https://openalex.org/W2077311988","https://openalex.org/W2624800021","https://openalex.org/W2007511049","https://openalex.org/W1999021161","https://openalex.org/W2064386449","https://openalex.org/W2088665387","https://openalex.org/W3172086192","https://openalex.org/W4407501763","https://openalex.org/W4281849153","https://openalex.org/W1987411651"],"abstract_inverted_index":{"Rat":[0],"parvalbumin":[1],"(PV),":[2],"an":[3],"EF-hand":[4],"type":[5],"Ca(2+)-binding":[6],"protein,":[7],"was":[8,132],"expressed":[9],"in":[10,26,188,228],"Escherichia":[11],"coli":[12],"and":[13,39,48,58,75,84,95,97,106,127,142,162,199],"mutated":[14],"by":[15,203],"replacing":[16],"a":[17,23,30,159],"Phe":[18],"at":[19,153],"position":[20,154],"102":[21,155],"with":[22,70],"unique":[24],"Trp":[25,139,151,179],"order":[27],"to":[28,134,158,211,224],"introduce":[29],"distinct":[31],"fluorescent":[32],"label":[33],"into":[34],"the":[35,44,54,60,119,129,150,171,175,178,217,225],"protein.":[36,230],"Mass":[37],"spectroscopy":[38],"NMR":[40],"data":[41],"indicate":[42],"that":[43,149,216],"recombinant":[45],"wild-type":[46],"(PVWT)":[47],"F102W":[49],"mutant":[50],"(PVF102W)":[51],"proteins":[52,64],"have":[53],"expected":[55],"molecular":[56],"weight":[57],"retain":[59],"native":[61],"structure.":[62],"Both":[63],"contain":[65],"two":[66],"non-cooperative":[67],"Ca2+/Mg(2+)-binding":[68],"sites":[69],"intrinsic":[71],"affinity":[72],"constants,":[73],"KCa":[74,96],"KMg,":[76,98],"of":[77,85,99,107,125,146,174,197,219],"2.4":[78],"+/-":[79,87,101,109],"0.9":[80],"x":[81,89,103,111],"10(7)":[82,104],"M-1":[83,105],"2.9":[86],"0.2":[88],"10(4)":[90,112],"M-1,":[91,113],"respectively,":[92,114],"for":[93,115],"PVWT,":[94],"2.7":[100],"1.1":[102],"4.4":[108],"0.3":[110],"PVF102W.":[116],"Based":[117],"on":[118],"highly":[120],"similar":[121],"metal":[122],"binding":[123,170,196,218],"properties":[124],"PVWT":[126],"PVF102W":[128,147],"latter":[130],"protein":[131],"used":[133],"study":[135],"cation-dependent":[136],"conformational":[137,193],"changes.":[138],"fluorescence":[140],"emission":[141],"UV":[143,204],"difference":[144,205],"spectra":[145],"indicated":[148],"residue":[152],"is":[156,180],"confined":[157],"hydrophobic":[160],"core":[161],"conformationally":[163],"strongly":[164],"restricted.":[165],"Upon":[166],"Ca2+":[167,198],"or":[168],"Mg2+":[169],"structural":[172,226],"organization":[173,227],"region":[176],"around":[177],"hardly":[181],"affected,":[182],"but":[183],"there":[184],"are":[185],"significant":[186],"changes":[187],"its":[189],"electrostatic":[190],"environment.":[191],"The":[192],"change":[194],"upon":[195],"Mg2+,":[200],"as":[201],"monitored":[202],"spectrophotometry,":[206],"increases":[207],"linearly":[208],"from":[209],"0":[210],"2":[212],"cations":[213],"bound,":[214],"indicating":[215],"both":[220],"ions":[221],"contributes":[222],"equally":[223],"this":[229]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}