{"id":"https://openalex.org/W1503295604","doi":"https://doi.org/10.1074/jbc.271.36.21713","title":"Purine-specific Nucleoside N-Ribohydrolase from Trypanosoma brucei brucei","display_name":"Purine-specific Nucleoside N-Ribohydrolase from Trypanosoma brucei brucei","publication_year":1996,"publication_date":"1996-09-01","ids":{"openalex":"https://openalex.org/W1503295604","doi":"https://doi.org/10.1074/jbc.271.36.21713","mag":"1503295604","pmid":"https://pubmed.ncbi.nlm.nih.gov/8702965"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.271.36.21713","doi":"https://doi.org/10.1074/jbc.271.36.21713","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.271.36.21713","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.271.36.21713","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003762466","display_name":"David W. Parkin","orcid":"https://orcid.org/0000-0003-4479-3164","observed_orcids":["https://orcid.org/0000-0003-4479-3164"]},"institutions":[{"id":"https://openalex.org/I58422712","display_name":"International Livestock Research Institute","ror":"https://ror.org/01jxjwb74","country_code":"KE","type":"facility","lineage":["https://openalex.org/I1286583668","https://openalex.org/I58422712"]}],"countries":["KE"],"is_corresponding":true,"raw_author_name":"David W. Parkin","raw_affiliation_strings":["\u2002International Livestock Research Institute, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u2002International Livestock Research Institute, Nairobi, Kenya","institution_ids":["https://openalex.org/I58422712"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5003762466"],"corresponding_institution_ids":["https://openalex.org/I58422712"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.9496,"has_fulltext":false,"cited_by_count":92,"citation_normalized_percentile":{"value":0.71402449,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"271","issue":"36","first_page":"21713","last_page":"21719"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.7768999934196472,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.7768999934196472,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10890","display_name":"Trypanosoma species research and implications","score":0.22089999914169312,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11193","display_name":"Adenosine and Purinergic Signaling","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trypanosoma-brucei-brucei","display_name":"Trypanosoma brucei brucei","score":0.9259999990463257},{"id":"https://openalex.org/keywords/nucleoside-hydrolase","display_name":"nucleoside hydrolase","score":0.9120000004768372},{"id":"https://openalex.org/keywords/purine-salvage-pathway","display_name":"purine salvage pathway","score":0.8050000071525574},{"id":"https://openalex.org/keywords/inosine","display_name":"inosine","score":0.7850000262260437},{"id":"https://openalex.org/keywords/adenosine","display_name":"adenosine","score":0.9779999852180481},{"id":"https://openalex.org/keywords/guanosine","display_name":"guanosine","score":0.9959999918937683},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.8539999723434448},{"id":"https://openalex.org/keywords/competitive-inhibition","display_name":"competitive inhibition","score":0.5569999814033508},{"id":"https://openalex.org/keywords/tubercidin","display_name":"tubercidin","score":0.8090000152587891},{"id":"https://openalex.org/keywords/formycin-b","display_name":"formycin B","score":0.9670000076293945},{"id":"https://openalex.org/keywords/3-deazaadenosine","display_name":"3-deazaadenosine","score":0.8759999871253967},{"id":"https://openalex.org/keywords/7-deazaadenosine","display_name":"7-deazaadenosine","score":0.9639999866485596},{"id":"https://openalex.org/keywords/ph-dependence","display_name":"pH dependence","score":0.578000009059906},{"id":"https://openalex.org/keywords/bloodstream-form-trypanosomes","display_name":"bloodstream form trypanosomes","score":0.5429999828338623}],"concepts":[{"id":"https://openalex.org/C2777610669","wikidata":"https://www.wikidata.org/wiki/Q422564","display_name":"Inosine","level":3,"score":0.9398411512374878},{"id":"https://openalex.org/C2779502636","wikidata":"https://www.wikidata.org/wiki/Q33244","display_name":"Trypanosoma brucei","level":3,"score":0.8451696634292603},{"id":"https://openalex.org/C71615608","wikidata":"https://www.wikidata.org/wiki/Q24720082","display_name":"Purine nucleoside phosphorylase","level":4,"score":0.7937232255935669},{"id":"https://openalex.org/C2776543447","wikidata":"https://www.wikidata.org/wiki/Q28734","display_name":"Nucleoside","level":2,"score":0.7817429304122925},{"id":"https://openalex.org/C2777995097","wikidata":"https://www.wikidata.org/wiki/Q422462","display_name":"Guanosine","level":2,"score":0.7567664384841919},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.6943700313568115},{"id":"https://openalex.org/C2780120296","wikidata":"https://www.wikidata.org/wiki/Q96286","display_name":"Hydrolase","level":3,"score":0.6170318126678467},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6162380576133728},{"id":"https://openalex.org/C34628245","wikidata":"https://www.wikidata.org/wiki/Q356093","display_name":"Adenosine deaminase","level":3,"score":0.5684312582015991},{"id":"https://openalex.org/C2776476023","wikidata":"https://www.wikidata.org/wiki/Q188261","display_name":"Purine","level":3,"score":0.5481045246124268},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5238121747970581},{"id":"https://openalex.org/C2776991684","wikidata":"https://www.wikidata.org/wiki/Q190012","display_name":"Adenosine","level":2,"score":0.4878849983215332},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.4687768518924713},{"id":"https://openalex.org/C169586020","wikidata":"https://www.wikidata.org/wiki/Q3780159","display_name":"Purine metabolism","level":3,"score":0.4674096405506134},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4570479691028595},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4519032835960388},{"id":"https://openalex.org/C2909748322","wikidata":"https://www.wikidata.org/wiki/Q255933","display_name":"Inosine monophosphate","level":4,"score":0.4452732503414154},{"id":"https://openalex.org/C2780636463","wikidata":"https://www.wikidata.org/wiki/Q422457","display_name":"Deoxyadenosine","level":3,"score":0.4131196439266205},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3880216181278229},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.28271013498306274},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007042","descriptor_name":"Hypoxanthines","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009699","descriptor_name":"N-Glycosyl Hydrolases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D009699","descriptor_name":"N-Glycosyl Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011683","descriptor_name":"Purine-Nucleoside Phosphorylase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012266","descriptor_name":"Ribose","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014346","descriptor_name":"Trypanosoma brucei brucei","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D019271","descriptor_name":"Hypoxanthine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1074/jbc.271.36.21713","doi":"https://doi.org/10.1074/jbc.271.36.21713","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.271.36.21713","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:8702965","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8702965","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:cgspace.cgiar.org:10568/32992","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/10568/32992","pdf_url":null,"source":{"id":"https://openalex.org/S4306402055","display_name":"CGSPace A Repository of Agricultural Research Outputs (Consultative Group for International Agricultural Research)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1286583668","host_organization_name":"CGIAR","host_organization_lineage":["https://openalex.org/I1286583668"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Parkin, D. W. (1996). Purine-specific Nucleoside N-Ribohydrolase from Trypanosoma brucei brucei. Journal of Biological Chemistry 271(36): 21713\u201321719. Elsevier BV. https://doi.org/10.1074/jbc.271.36.21713","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.271.36.21713","doi":"https://doi.org/10.1074/jbc.271.36.21713","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.271.36.21713","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W103819310","https://openalex.org/W1496737953","https://openalex.org/W1497167336","https://openalex.org/W1513458978","https://openalex.org/W1522363773","https://openalex.org/W1571253423","https://openalex.org/W1889077695","https://openalex.org/W1978289046","https://openalex.org/W1982582769","https://openalex.org/W1987770670","https://openalex.org/W1992760440","https://openalex.org/W1995600978","https://openalex.org/W1998870863","https://openalex.org/W2005509656","https://openalex.org/W2051992969","https://openalex.org/W2063330286","https://openalex.org/W2084434937","https://openalex.org/W2084498540","https://openalex.org/W2092327285","https://openalex.org/W2092494663","https://openalex.org/W2095263150","https://openalex.org/W2135602522","https://openalex.org/W2152192192","https://openalex.org/W2403297264","https://openalex.org/W2613467369","https://openalex.org/W2969273703","https://openalex.org/W4234513436","https://openalex.org/W4254651107"],"related_works":["https://openalex.org/W2086938562","https://openalex.org/W2187510843","https://openalex.org/W1977376296","https://openalex.org/W7125882171","https://openalex.org/W2057704501","https://openalex.org/W2018862779","https://openalex.org/W2055961804","https://openalex.org/W2436569602","https://openalex.org/W1496737953","https://openalex.org/W1497167336"],"abstract_inverted_index":{"Trypanosomes":[0],"have":[1],"no":[2,161],"de":[3],"novo":[4],"purine":[5,163],"biosynthesis":[6,23],"and":[7,20,82,91,158,170,181,237],"thus":[8],"depend":[9],"upon":[10],"salvage":[11],"pathways":[12,19],"to":[13,58],"obtain":[14],"purines":[15],"for":[16,21,79,116,212,258],"their":[17],"metabolic":[18],"the":[22,35,45,62,213,216,247],"of":[24,44,65,73,133,178,215,235,244],"nucleic":[25],"acids.":[26],"An":[27],"inosine-adenosine-guanosine":[28],"preferring":[29],"nucleoside":[30,164],"hydrolase":[31,69],"(IAG-nucleoside":[32],"hydrolase)":[33],"from":[34,61],"African":[36],"trypanosome":[37],"Trypanosoma":[38],"brucei":[39,40],"represents":[41],"approximately":[42,134],"0.2%":[43],"soluble":[46],"protein":[47],"in":[48,227],"this":[49,66],"organism.":[50],"The":[51,77,99,113,185,199,219],"enzyme":[52],"has":[53],"been":[54],"purified":[55],"over":[56],"400-fold":[57],">95%":[59],"homogeneity":[60],"bloodstream":[63],"form":[64],"parasite.":[67],"IAG-nucleoside":[68],"is":[70,106,122,153,160],"a":[71,155,188,194,238,242],"dimer":[72],"Mr":[74],"36,000":[75],"subunits.":[76],"kcat/Km":[78,131,145],"inosine,":[80],"adenosine,":[81],"guanosine":[83],"are":[84,127,140,173,204],"1.9":[85],"x":[86,89,93],"10(6),":[87,90],"1.2":[88],"0.83":[92],"10(6)":[94],"M":[95,136,149],"-1":[96,137,150],"s-1,":[97],"respectively.":[98,184],"kinetic":[100],"mechanism":[101],"with":[102,109,130,144,176,193,241],"inosine":[103,117],"as":[104],"substrate":[105,157],"rapid":[107],"equilibrium":[108],"random":[110],"product":[111],"release.":[112],"turnover":[114],"rate":[115],"at":[118],"30":[119],"degrees":[120],"C":[121],"34":[123],"s-1.":[124,138,151],"Pyrimidine":[125],"nucleosides":[126],"poor":[128,142],"substrates":[129,143],"values":[132,146,234],"10(3)":[135],"Deoxynucleosides":[139],"also":[141],"near":[147],"10(2)":[148],"AMP":[152],"not":[154],"detectable":[156],"there":[159,203],"measurable":[162],"phosphorylase":[165],"activity.":[166,261],"3-Deazaadenosine,":[167],"7-deazaadenosine":[168],"(tubercidin),":[169],"formycin":[171],"B":[172],"competitive":[174],"inhibitors":[175],"Kis":[177],"1.8,":[179],"59,":[180],"13":[182],"microM,":[183],"Km":[186],"shows":[187],"slight":[189],"dependence":[190],"on":[191],"pH":[192,195],"optimum":[196,259],"around":[197],"7.":[198],"Vmax/Km":[200],"data":[201,221],"indicate":[202,222],"two":[205],"ionizable":[206,224],"enzymatic":[207],"groups,":[208],"pKa":[209,233,243],"8.6,":[210],"required":[211],"formation":[214],"Michaelis":[217],"complex.":[218],"Vmax":[220,248],"three":[223,253],"groups":[225,231,254],"involved":[226],"catalysis.":[228],"Two":[229],"essential":[230],"exhibit":[232],"8.8,":[236],"third":[239],"group":[240],"6.5":[245],"increases":[246],"an":[249],"additional":[250],"10-fold.":[251],"All":[252],"must":[255],"be":[256],"protonated":[257],"catalytic":[260]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}