{"id":"https://openalex.org/W1502492430","doi":"https://doi.org/10.1128/jvi.23.3.616-625.1977","title":"Adenovirus Type 2 Early Nuclear and mRNA: Kinetic Estimation of l and r DNA Strand Fractions Complementary to Different Abundance Classes of Viral RNA","display_name":"Adenovirus Type 2 Early Nuclear and mRNA: Kinetic Estimation of l and r DNA Strand Fractions Complementary to Different Abundance Classes of Viral RNA","publication_year":1977,"publication_date":"1977-09-01","ids":{"openalex":"https://openalex.org/W1502492430","doi":"https://doi.org/10.1128/jvi.23.3.616-625.1977","mag":"1502492430","pmid":"https://pubmed.ncbi.nlm.nih.gov/894792","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/515873"},"language":"en","primary_location":{"id":"doi:10.1128/jvi.23.3.616-625.1977","doi":"https://doi.org/10.1128/jvi.23.3.616-625.1977","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.23.3.616-625.1977","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/515873","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032387026","display_name":"William S.M. Wold","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47838141","display_name":"Saint Louis University","ror":"https://ror.org/01p7jjy08","country_code":"US","type":"education","lineage":["https://openalex.org/I47838141"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William S. M. Wold","raw_affiliation_strings":["Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I47838141"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103398972","display_name":"Maurice Green","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47838141","display_name":"Saint Louis University","ror":"https://ror.org/01p7jjy08","country_code":"US","type":"education","lineage":["https://openalex.org/I47838141"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maurice Green","raw_affiliation_strings":["Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I47838141"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044303453","display_name":"Karl H. Brackmann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47838141","display_name":"Saint Louis University","ror":"https://ror.org/01p7jjy08","country_code":"US","type":"education","lineage":["https://openalex.org/I47838141"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karl H. Brackmann","raw_affiliation_strings":["Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I47838141"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053837145","display_name":"Catherine S. Devine","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47838141","display_name":"Saint Louis University","ror":"https://ror.org/01p7jjy08","country_code":"US","type":"education","lineage":["https://openalex.org/I47838141"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Catherine Devine","raw_affiliation_strings":["Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I47838141"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022176731","display_name":"Maria A. Cartas","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I47838141","display_name":"Saint Louis University","ror":"https://ror.org/01p7jjy08","country_code":"US","type":"education","lineage":["https://openalex.org/I47838141"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maria A. Cartas","raw_affiliation_strings":["Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Molecular Virology, Saint Louis University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I47838141"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I47838141"],"apc_list":null,"apc_paid":null,"fwci":0.9282,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.71951543,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"23","issue":"3","first_page":"616","last_page":"625"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.33889999985694885,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.33889999985694885,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.14579999446868896,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.1062999963760376,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adenovirus-type-2","display_name":"adenovirus type 2","score":0.9819999933242798},{"id":"https://openalex.org/keywords/early-mrna","display_name":"early mRNA","score":0.6859999895095825},{"id":"https://openalex.org/keywords/nuclear-rna","display_name":"nuclear RNA","score":0.8420000076293945},{"id":"https://openalex.org/keywords/early-gene-expression","display_name":"early gene expression","score":0.3840000033378601},{"id":"https://openalex.org/keywords/kb-cells","display_name":"KB cells","score":0.5450000166893005},{"id":"https://openalex.org/keywords/rna-polymerase-iii","display_name":"RNA polymerase III","score":0.5910000205039978},{"id":"https://openalex.org/keywords/rna-polymerase-i","display_name":"RNA polymerase I","score":0.3540000021457672}],"concepts":[{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.8683381080627441},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7927453517913818},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6536929607391357},{"id":"https://openalex.org/C108190873","wikidata":"https://www.wikidata.org/wiki/Q6395811","display_name":"Nuclease protection assay","level":5,"score":0.6500388979911804},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6349344253540039},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.46289318799972534},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.4304332733154297},{"id":"https://openalex.org/C2780114586","wikidata":"https://www.wikidata.org/wiki/Q40260","display_name":"Cell nucleus","level":3,"score":0.4107389748096466},{"id":"https://openalex.org/C41258723","wikidata":"https://www.wikidata.org/wiki/Q2919111","display_name":"RNA-dependent RNA polymerase","level":4,"score":0.32156819105148315},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3182241916656494},{"id":"https://openalex.org/C190062978","wikidata":"https://www.wikidata.org/wiki/Q79899","display_name":"Cytoplasm","level":2,"score":0.29973676800727844},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1749076247215271},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000260","descriptor_name":"Adenoviruses, Human","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011132","descriptor_name":"Polyribosomes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D012334","descriptor_name":"RNA, Neoplasm","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D012335","descriptor_name":"RNA, Ribosomal","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1128/jvi.23.3.616-625.1977","doi":"https://doi.org/10.1128/jvi.23.3.616-625.1977","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.23.3.616-625.1977","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:515873","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/515873","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},{"id":"pmid:894792","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/894792","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:515873","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/515873","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1480223204","https://openalex.org/W1484418052","https://openalex.org/W1542067355","https://openalex.org/W1560044106","https://openalex.org/W1564872407","https://openalex.org/W1597417422","https://openalex.org/W1977452821","https://openalex.org/W1977530857","https://openalex.org/W1984427697","https://openalex.org/W1986427370","https://openalex.org/W2002412830","https://openalex.org/W2013865697","https://openalex.org/W2017940381","https://openalex.org/W2042452022","https://openalex.org/W2058109710","https://openalex.org/W2083286064","https://openalex.org/W2083950790","https://openalex.org/W2105846281","https://openalex.org/W2108703593","https://openalex.org/W2150653661","https://openalex.org/W2159862341","https://openalex.org/W2160169856","https://openalex.org/W2314898622","https://openalex.org/W2598971889"],"related_works":["https://openalex.org/W1480223204","https://openalex.org/W1986427370","https://openalex.org/W1564872407","https://openalex.org/W2109405361","https://openalex.org/W1548849373","https://openalex.org/W1626472837","https://openalex.org/W1906081351","https://openalex.org/W2005566242","https://openalex.org/W1484418052","https://openalex.org/W2433504112"],"abstract_inverted_index":{"RNA":[0,77,80,131,134,168,171,177,263,313,316],"from":[1,9,60],"unfractionated":[2],"cells,":[3],"nuclei,":[4],"and":[5,18,30,56,78,84,89,99,108,116,121,132,142,169,191,200,206,215,236,270,294,301],"polyribosomes":[6],"was":[7],"extracted":[8],"adenovirus":[10],"2":[11],"(Ad2)-infected":[12],"KB":[13],"cells":[14],"early":[15,309,352],"in":[16,20,23,348],"infection":[17],"annealed":[19,81,158],"vast":[21],"excess":[22],"liquid":[24],"to":[25,40,82,104,106,110,117,119,123,140,144,159,179,185,197,209,213,227,281,292,297,305],"purified":[26],"Ad2":[27,51,315],"l":[28,88,114,204,300],"(heavy)":[29],"r":[31,90,127,219,302],"(light)":[32],"[(32)P]DNA":[33],"strands":[34,328],"(specific":[35],"activity,":[36],"3":[37],"x":[38,42],"10(6)":[39],"1.5":[41],"10(7)":[43],"cpm/mug).":[44],"The":[45,221,268],"number":[46],"of":[47,50,72,87,113,126,162,203,218,250,265,286,299,325,351],"abundance":[48,93,183],"classes":[49,95,101,137,184,223,235,252,259,272],"RNA,":[52],"their":[53],"relative":[54],"concentrations,":[55],"the":[57,135,233,242,247,256,283,349],"strand":[58,205,289],"fraction":[59,285],"which":[61],"they":[62,278],"arose":[63],"were":[64,96,102,138,155,174,224],"determined":[65],"by":[66],"a":[67],"computer-assisted":[68],"nonlinear":[69],"regression":[70],"analysis":[71],"hybridization":[73,172],"kinetic":[74],"data.":[75],"Whole-cell":[76],"nuclear":[79,133,170,262,312],"60":[83],"40%,":[85],"respectively,":[86,112,125,149,195],"strands.":[91],"Well-defined":[92],"(kinetic)":[94],"identified:":[97],"abundant":[98,136,222,269],"scarce":[100,151,193,234,244,258,271],"complementary":[103,280],"15":[105],"17":[107,122],"40":[109],"45%,":[111],"strand,":[115],"11":[118],"16":[120],"23%,":[124],"strand.":[128,220],"In":[129],"whole-cell":[130,167],"57":[139],"208":[141],"13":[143],"27":[145],"times":[146,229,238],"more":[147,230,239],"concentrated,":[148],"than":[150,232,241],"classes.":[152,245],"RNA-RNA":[153],"hybrids":[154],"isolated":[156],"that":[157,166],"about":[160,201,216,282],"70%":[161],"both":[163,326],"strands,":[164],"indicating":[165],"values":[173],"minimal.":[175],"Polyribosomal":[176],"appeared":[178],"anneal":[180],"as":[181,308],"three":[182],"each":[186,287],"DNA":[187,288,327],"strand;":[188],"abundant,":[189],"scarce,":[190],"very":[192,243,257],"classes,":[194],"hybridized":[196],"6,":[198],"5,":[199],"10%":[202],"7":[207],"(6":[208],"8),":[210],"10":[211],"(8":[212],"13),":[214],"19%":[217],"41":[225],"(11":[226],"67)":[228],"concentrated":[231,240],"10(3)":[237],"Although":[246],"biological":[248],"significance":[249],"these":[251],"is":[253,340],"not":[254,318,341],"known,":[255],"probably":[260],"represent":[261],"contaminants":[264],"polyribosomal":[266],"RNA.":[267],"may":[273],"comprise":[274],"mRNA,":[275],"because":[276],"together":[277],"are":[279,329,335,346],"same":[284],"(11%":[290],"[10":[291],"12%]":[293],"17%":[295],"[14":[296],"20%]":[298],"strands)":[303],"known":[304,342],"be":[306],"expressed":[307],"mRNA.":[310,338],"Thus,":[311],"contains":[314],"sequences":[317],"found":[319],"on":[320],"polyribosomes;":[321],"most":[322],"or":[323],"all":[324],"transcribed,":[330],"but":[331],"only":[332],"certain":[333],"transcripts":[334,345],"processed":[336],"into":[337],"It":[339],"whether":[343],"\"non-mRNA\"":[344],"intermediates":[347],"pathway":[350],"mRNA":[353],"production.":[354]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}