{"id":"https://openalex.org/W1502011944","doi":"https://doi.org/10.1182/blood.v31.1.66.66","title":"Ficoll Flotation for the Separation of Blood Leukocyte Types","display_name":"Ficoll Flotation for the Separation of Blood Leukocyte Types","publication_year":1968,"publication_date":"1968-01-01","ids":{"openalex":"https://openalex.org/W1502011944","doi":"https://doi.org/10.1182/blood.v31.1.66.66","mag":"1502011944","pmid":"https://pubmed.ncbi.nlm.nih.gov/4966278"},"language":"en","primary_location":{"id":"doi:10.1182/blood.v31.1.66.66","doi":"https://doi.org/10.1182/blood.v31.1.66.66","is_oa":true,"landing_page_url":"https://doi.org/10.1182/blood.v31.1.66.66","pdf_url":"https://ashpublications.org/blood/article-pdf/31/1/66/574040/66.pdf","source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://ashpublications.org/blood/article-pdf/31/1/66/574040/66.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025926124","display_name":"P. B. NOBLE","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I125749732","display_name":"Western University","ror":"https://ror.org/02grkyz14","country_code":"CA","type":"education","lineage":["https://openalex.org/I125749732"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"P. B. NOBLE","raw_affiliation_strings":["Collip Medical Research Laboratory and Department of Anatomy, Health Sciences Center, University of Western Ontario, London, Ontario, Canada","Collip Medical Research Laboratory, University of Western Ontario, London, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Collip Medical Research Laboratory and Department of Anatomy, Health Sciences Center, University of Western Ontario, London, Ontario, Canada","institution_ids":["https://openalex.org/I125749732"]},{"raw_affiliation_string":"Collip Medical Research Laboratory, University of Western Ontario, London, Ontario, Canada","institution_ids":["https://openalex.org/I125749732"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111481774","display_name":"James H. Cutts","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I125749732","display_name":"Western University","ror":"https://ror.org/02grkyz14","country_code":"CA","type":"education","lineage":["https://openalex.org/I125749732"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"J. HARRY CUTTS","raw_affiliation_strings":["Collip Medical Research Laboratory and Department of Anatomy, Health Sciences Center, University of Western Ontario, London, Ontario, Canada","Department of Anatomy and Cancer Research Laboratory, University of Western Ontario, London, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Collip Medical Research Laboratory and Department of Anatomy, Health Sciences Center, University of Western Ontario, London, Ontario, Canada","institution_ids":["https://openalex.org/I125749732"]},{"raw_affiliation_string":"Department of Anatomy and Cancer Research Laboratory, University of Western Ontario, London, Ontario, Canada","institution_ids":["https://openalex.org/I125749732"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111048456","display_name":"K. K. Carroll","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I125749732","display_name":"Western University","ror":"https://ror.org/02grkyz14","country_code":"CA","type":"education","lineage":["https://openalex.org/I125749732"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"K. K. CARROLL","raw_affiliation_strings":["Collip Medical Research Laboratory and Department of Anatomy, Health Sciences Center, University of Western Ontario, London, Ontario, Canada","Department of Medical Research, University of Western Ontario, London, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Collip Medical Research Laboratory and Department of Anatomy, Health Sciences Center, University of Western Ontario, London, Ontario, Canada","institution_ids":["https://openalex.org/I125749732"]},{"raw_affiliation_string":"Department of Medical Research, University of Western Ontario, London, Ontario, Canada","institution_ids":["https://openalex.org/I125749732"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I125749732"],"apc_list":{"value":5500,"currency":"USD","value_usd":5500},"apc_paid":null,"fwci":17.0657,"has_fulltext":true,"cited_by_count":77,"citation_normalized_percentile":{"value":0.99177877,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"31","issue":"1","first_page":"66","last_page":"73"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.1306000053882599,"subfield":{"id":"https://openalex.org/subfields/3607","display_name":"Medical Laboratory Technology"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.1306000053882599,"subfield":{"id":"https://openalex.org/subfields/3607","display_name":"Medical Laboratory Technology"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.1136000007390976,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.10329999774694443,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ficoll-density-gradient","display_name":"Ficoll density gradient","score":0.8190000057220459},{"id":"https://openalex.org/keywords/leukocyte-separation","display_name":"leukocyte separation","score":0.9789999723434448},{"id":"https://openalex.org/keywords/peripheral-blood","display_name":"peripheral blood","score":0.6200000047683716},{"id":"https://openalex.org/keywords/leukocyte-subtypes","display_name":"leukocyte subtypes","score":0.6259999871253967},{"id":"https://openalex.org/keywords/leukocyte-isolation","display_name":"leukocyte isolation","score":0.5590000152587891},{"id":"https://openalex.org/keywords/phagocytosis","display_name":"phagocytosis","score":0.8220000267028809},{"id":"https://openalex.org/keywords/leukocyte-motility","display_name":"leukocyte motility","score":0.6899999976158142}],"concepts":[{"id":"https://openalex.org/C5362183","wikidata":"https://www.wikidata.org/wiki/Q410200","display_name":"Ficoll","level":4,"score":0.8880826234817505},{"id":"https://openalex.org/C160448771","wikidata":"https://www.wikidata.org/wiki/Q184726","display_name":"Phagocytosis","level":2,"score":0.625024676322937},{"id":"https://openalex.org/C3017910909","wikidata":"https://www.wikidata.org/wiki/Q618363","display_name":"Peripheral blood","level":2,"score":0.5316420793533325},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4849396049976349},{"id":"https://openalex.org/C2776061190","wikidata":"https://www.wikidata.org/wiki/Q7451805","display_name":"Separation (statistics)","level":2,"score":0.4315418601036072},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42072415351867676},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3437035083770752},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1296132504940033},{"id":"https://openalex.org/C137061746","wikidata":"https://www.wikidata.org/wiki/Q736033","display_name":"Peripheral blood mononuclear cell","level":3,"score":0.12230217456817627},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.10119548439979553},{"id":"https://openalex.org/C119857082","wikidata":"https://www.wikidata.org/wiki/Q2539","display_name":"Machine learning","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001792","descriptor_name":"Blood Platelets","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D002498","descriptor_name":"Centrifugation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004492","descriptor_name":"Edetic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004912","descriptor_name":"Erythrocytes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006493","descriptor_name":"Heparin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007962","descriptor_name":"Leukocytes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008214","descriptor_name":"Lymphocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D008722","descriptor_name":"Methods","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008858","descriptor_name":"Microscopy, Phase-Contrast","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009000","descriptor_name":"Monocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D013395","descriptor_name":"Sucrose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1182/blood.v31.1.66.66","doi":"https://doi.org/10.1182/blood.v31.1.66.66","is_oa":true,"landing_page_url":"https://doi.org/10.1182/blood.v31.1.66.66","pdf_url":"https://ashpublications.org/blood/article-pdf/31/1/66/574040/66.pdf","source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},{"id":"pmid:4966278","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4966278","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.927.3950","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.927.3950","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.bloodjournal.org/content/bloodjournal/31/1/66.full.pdf?sso-checked%3Dtrue","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1182/blood.v31.1.66.66","doi":"https://doi.org/10.1182/blood.v31.1.66.66","is_oa":true,"landing_page_url":"https://doi.org/10.1182/blood.v31.1.66.66","pdf_url":"https://ashpublications.org/blood/article-pdf/31/1/66/574040/66.pdf","source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1502011944.pdf","grobid_xml":"https://content.openalex.org/works/W1502011944.grobid-xml"},"referenced_works_count":10,"referenced_works":["https://openalex.org/W199122950","https://openalex.org/W1574632964","https://openalex.org/W2017202628","https://openalex.org/W2135522301","https://openalex.org/W2257840380","https://openalex.org/W2316139571","https://openalex.org/W2335703178","https://openalex.org/W2413325566","https://openalex.org/W2413336505","https://openalex.org/W2419889054"],"related_works":["https://openalex.org/W2464428080","https://openalex.org/W199122950","https://openalex.org/W2419889054","https://openalex.org/W6017993","https://openalex.org/W2306856764","https://openalex.org/W2413325566","https://openalex.org/W2005025247","https://openalex.org/W2068180652","https://openalex.org/W2472757390","https://openalex.org/W4313373538"],"abstract_inverted_index":{"Abstract":[0],"A":[1],"method":[2,18],"for":[3],"the":[4,25],"separation":[5],"of":[6,31,39],"leukocytes":[7,26],"from":[8],"peripheral":[9],"blood,":[10],"using":[11],"flotation":[12],"over":[13],"Ficoll,":[14],"is":[15,19],"described.":[16],"The":[17],"amenable":[20],"to":[21],"considerable":[22],"variation,":[23],"and":[24,37],"isolated":[27],"show":[28],"good":[29],"preservation":[30],"their":[32],"morphologic":[33],"integrity,":[34],"are":[35],"motile":[36],"capable":[38],"phagocytosis.":[40]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}