{"id":"https://openalex.org/W1500767321","doi":null,"title":"Scaffolding functions of MAGI-2 in the PTEN mediated attenuation of the PI3K/Akt signalling pathway","display_name":"Scaffolding functions of MAGI-2 in the PTEN mediated attenuation of the PI3K/Akt signalling pathway","publication_year":2009,"publication_date":"2009-09-01","ids":{"openalex":"https://openalex.org/W1500767321","mag":"1500767321"},"language":"en","primary_location":{"id":"pmh:oai:ecommons.usask.ca:10388/etd-09172009-110738","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-09172009-110738","pdf_url":"https://harvest.usask.ca/bitstreams/9fb06f54-63b9-4823-8afe-b8ac2f16c81d/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://harvest.usask.ca/bitstreams/9fb06f54-63b9-4823-8afe-b8ac2f16c81d/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042394487","display_name":"Sharon Franceska Poland","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Sharon Franceska Poland","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5042394487"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10952","display_name":"PI3K/AKT/mTOR signaling in cancer","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pten","display_name":"PTEN","score":0.9869999885559082},{"id":"https://openalex.org/keywords/pi3k-akt-signaling","display_name":"PI3K/Akt signaling","score":0.9419999718666077},{"id":"https://openalex.org/keywords/pdz-domain","display_name":"PDZ domain","score":0.7429999709129333},{"id":"https://openalex.org/keywords/ww-domain","display_name":"WW domain","score":0.7760000228881836},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.734000027179718},{"id":"https://openalex.org/keywords/p85","display_name":"p85","score":0.5550000071525574},{"id":"https://openalex.org/keywords/egfr","display_name":"EGFR","score":0.6549999713897705},{"id":"https://openalex.org/keywords/pdgfr","display_name":"PDGFR","score":0.8399999737739563},{"id":"https://openalex.org/keywords/receptor-tyrosine-kinase-signaling","display_name":"receptor tyrosine kinase signaling","score":0.7480000257492065},{"id":"https://openalex.org/keywords/scaffold-proteins","display_name":"scaffold proteins","score":0.6259999871253967},{"id":"https://openalex.org/keywords/lipid-second-messengers","display_name":"lipid second messengers","score":0.6700000166893005},{"id":"https://openalex.org/keywords/phosphatidylinositol-3-4-5-trisphosphate","display_name":"phosphatidylinositol 3,4,5-trisphosphate","score":0.9309999942779541},{"id":"https://openalex.org/keywords/tumour-suppressor","display_name":"tumour suppressor","score":0.5899999737739563}],"concepts":[{"id":"https://openalex.org/C2777609662","wikidata":"https://www.wikidata.org/wiki/Q416356","display_name":"PTEN","level":4,"score":0.8559252023696899},{"id":"https://openalex.org/C86554907","wikidata":"https://www.wikidata.org/wiki/Q285613","display_name":"PI3K/AKT/mTOR pathway","level":3,"score":0.7177321314811707},{"id":"https://openalex.org/C75217442","wikidata":"https://www.wikidata.org/wiki/Q423650","display_name":"Protein kinase B","level":3,"score":0.5519513487815857},{"id":"https://openalex.org/C39743133","wikidata":"https://www.wikidata.org/wiki/Q3262595","display_name":"Signalling","level":2,"score":0.5457394123077393},{"id":"https://openalex.org/C53645450","wikidata":"https://www.wikidata.org/wiki/Q907093","display_name":"Scaffold protein","level":3,"score":0.536332368850708},{"id":"https://openalex.org/C89429830","wikidata":"https://www.wikidata.org/wiki/Q735710","display_name":"Scaffold","level":2,"score":0.4722145199775696},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.45793747901916504},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.3870222568511963},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36401310563087463},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.3525288701057434},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.30204737186431885},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.28490328788757324},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:ecommons.usask.ca:10388/etd-09172009-110738","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-09172009-110738","pdf_url":"https://harvest.usask.ca/bitstreams/9fb06f54-63b9-4823-8afe-b8ac2f16c81d/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},{"id":"pmh:oai:harvest.usask.ca:10388/etd-09172009-110738","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10388/etd-09172009-110738","pdf_url":null,"source":{"id":"https://openalex.org/S7407063267","display_name":"HARVEST (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:1500767321","doi":null,"is_oa":false,"landing_page_url":"https://harvest.usask.ca/bitstream/10388/etd-09172009-110738/1/Poland.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:ecommons.usask.ca:10388/etd-09172009-110738","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10388/etd-09172009-110738","pdf_url":"https://harvest.usask.ca/bitstreams/9fb06f54-63b9-4823-8afe-b8ac2f16c81d/download","source":{"id":"https://openalex.org/S4377196608","display_name":"University Library - University of Saskatchewan (University of Saskatchewan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32625721","host_organization_name":"University of Saskatchewan","host_organization_lineage":["https://openalex.org/I32625721"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320317257","display_name":"Saskatchewan Cancer Agency","ror":"https://ror.org/00e1nmf62"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1500767321.pdf","grobid_xml":"https://content.openalex.org/works/W1500767321.grobid-xml"},"referenced_works_count":209,"referenced_works":["https://openalex.org/W71532689","https://openalex.org/W112874920","https://openalex.org/W146375978","https://openalex.org/W248588628","https://openalex.org/W274236614","https://openalex.org/W392968786","https://openalex.org/W1493363674","https://openalex.org/W1503953068","https://openalex.org/W1521412534","https://openalex.org/W1526353609","https://openalex.org/W1591206079","https://openalex.org/W1600477541","https://openalex.org/W1624747402","https://openalex.org/W1672544146","https://openalex.org/W1748445510","https://openalex.org/W1890918162","https://openalex.org/W1963795425","https://openalex.org/W1964330366","https://openalex.org/W1965655596","https://openalex.org/W1966032423","https://openalex.org/W1966658428","https://openalex.org/W1969366020","https://openalex.org/W1970439416","https://openalex.org/W1970671298","https://openalex.org/W1971447541","https://openalex.org/W1972172786","https://openalex.org/W1973777839","https://openalex.org/W1975306052","https://openalex.org/W1976028949","https://openalex.org/W1979187651","https://openalex.org/W1980943354","https://openalex.org/W1981041370","https://openalex.org/W1983268824","https://openalex.org/W1984418041","https://openalex.org/W1984647008","https://openalex.org/W1985516841","https://openalex.org/W1987086612","https://openalex.org/W1988594478","https://openalex.org/W1988769784","https://openalex.org/W1988784597","https://openalex.org/W1990061019","https://openalex.org/W1990999658","https://openalex.org/W1993238323","https://openalex.org/W1995116772","https://openalex.org/W1996320892","https://openalex.org/W1997245575","https://openalex.org/W1997341376","https://openalex.org/W1997990960","https://openalex.org/W1998088031","https://openalex.org/W1999752453","https://openalex.org/W1999814492","https://openalex.org/W2001130216","https://openalex.org/W2003231725","https://openalex.org/W2005051039","https://openalex.org/W2005747738","https://openalex.org/W2009126761","https://openalex.org/W2010286320","https://openalex.org/W2012825006","https://openalex.org/W2013964185","https://openalex.org/W2014278350","https://openalex.org/W2014390733","https://openalex.org/W2015889211","https://openalex.org/W2015908833","https://openalex.org/W2015926136","https://openalex.org/W2016248299","https://openalex.org/W2016398041","https://openalex.org/W2017141883","https://openalex.org/W2017191360","https://openalex.org/W2018146224","https://openalex.org/W2018229588","https://openalex.org/W2019421676","https://openalex.org/W2020302409","https://openalex.org/W2020891498","https://openalex.org/W2021151177","https://openalex.org/W2021153052","https://openalex.org/W2021462093","https://openalex.org/W2021717885","https://openalex.org/W2021766527","https://openalex.org/W2023575363","https://openalex.org/W2024028178","https://openalex.org/W2024210610","https://openalex.org/W2025845611","https://openalex.org/W2027470864","https://openalex.org/W2028415754","https://openalex.org/W2028587304","https://openalex.org/W2028826413","https://openalex.org/W2029815031","https://openalex.org/W2029946033","https://openalex.org/W2030899725","https://openalex.org/W2030986942","https://openalex.org/W2032244100","https://openalex.org/W2034828531","https://openalex.org/W2035437790","https://openalex.org/W2038422179","https://openalex.org/W2038545147","https://openalex.org/W2039132353","https://openalex.org/W2039589725","https://openalex.org/W2041293328","https://openalex.org/W2042199411","https://openalex.org/W2045533605","https://openalex.org/W2047118157","https://openalex.org/W2050039768","https://openalex.org/W2051403769","https://openalex.org/W2055043387","https://openalex.org/W2055345063","https://openalex.org/W2055405116","https://openalex.org/W2057847115","https://openalex.org/W2058620780","https://openalex.org/W2060324504","https://openalex.org/W2064189659","https://openalex.org/W2064258134","https://openalex.org/W2065514516","https://openalex.org/W2065796798","https://openalex.org/W2065989745","https://openalex.org/W2068240232","https://openalex.org/W2069802287","https://openalex.org/W2072697288","https://openalex.org/W2072846146","https://openalex.org/W2074570440","https://openalex.org/W2077642701","https://openalex.org/W2078452348","https://openalex.org/W2079516617","https://openalex.org/W2079977894","https://openalex.org/W2080694884","https://openalex.org/W2080711919","https://openalex.org/W2083682993","https://openalex.org/W2083879234","https://openalex.org/W2084413346","https://openalex.org/W2084531881","https://openalex.org/W2084944508","https://openalex.org/W2085826323","https://openalex.org/W2087883868","https://openalex.org/W2088028188","https://openalex.org/W2088138181","https://openalex.org/W2088239473","https://openalex.org/W2089412998","https://openalex.org/W2089512569","https://openalex.org/W2090948229","https://openalex.org/W2091218015","https://openalex.org/W2091848990","https://openalex.org/W2092806373","https://openalex.org/W2093853889","https://openalex.org/W2094957750","https://openalex.org/W2095636141","https://openalex.org/W2096014925","https://openalex.org/W2097097666","https://openalex.org/W2098106677","https://openalex.org/W2099658407","https://openalex.org/W2100837269","https://openalex.org/W2101444664","https://openalex.org/W2101625485","https://openalex.org/W2102394165","https://openalex.org/W2102424043","https://openalex.org/W2103093241","https://openalex.org/W2103994181","https://openalex.org/W2107213654","https://openalex.org/W2107804881","https://openalex.org/W2111973363","https://openalex.org/W2113561514","https://openalex.org/W2114725617","https://openalex.org/W2116354283","https://openalex.org/W2117996979","https://openalex.org/W2120357935","https://openalex.org/W2120587971","https://openalex.org/W2122706875","https://openalex.org/W2122829982","https://openalex.org/W2123403344","https://openalex.org/W2124157676","https://openalex.org/W2126778256","https://openalex.org/W2128368447","https://openalex.org/W2129221351","https://openalex.org/W2129546321","https://openalex.org/W2130867126","https://openalex.org/W2131089409","https://openalex.org/W2132178599","https://openalex.org/W2133115395","https://openalex.org/W2136164908","https://openalex.org/W2136205584","https://openalex.org/W2137578684","https://openalex.org/W2137884382","https://openalex.org/W2138540786","https://openalex.org/W2142159344","https://openalex.org/W2142507343","https://openalex.org/W2142873050","https://openalex.org/W2143404676","https://openalex.org/W2145080853","https://openalex.org/W2145195217","https://openalex.org/W2147855369","https://openalex.org/W2149638985","https://openalex.org/W2155298230","https://openalex.org/W2155737059","https://openalex.org/W2160260982","https://openalex.org/W2160537081","https://openalex.org/W2160675155","https://openalex.org/W2162095292","https://openalex.org/W2163012130","https://openalex.org/W2164443997","https://openalex.org/W2167013762","https://openalex.org/W2167152410","https://openalex.org/W2167573033","https://openalex.org/W2167618702","https://openalex.org/W2170171174","https://openalex.org/W2170296344","https://openalex.org/W2170379027","https://openalex.org/W2170416930","https://openalex.org/W2312397356","https://openalex.org/W2415763114","https://openalex.org/W2422187119","https://openalex.org/W2614940013"],"related_works":["https://openalex.org/W4230427059","https://openalex.org/W2329115852","https://openalex.org/W2050039768","https://openalex.org/W2088138181","https://openalex.org/W2085641812","https://openalex.org/W2023575363","https://openalex.org/W2075211183","https://openalex.org/W2085826323","https://openalex.org/W1964643818","https://openalex.org/W2302253684"],"abstract_inverted_index":{"Activated":[0],"receptor":[1,12,20],"tyrosine":[2],"kinase":[3],"(RTK),":[4],"such":[5,346],"as":[6,68,70,224,347],"the":[7,15,46,85,102,115,150,166,177,188,191,196,202,210,215,294,300,342,382,387,440,444,447],"epidermal":[8],"growth":[9,17,399,411],"factor":[10,18,400,412],"(EGF)":[11],"(EGFR)":[13],"and":[14,37,66,73,136,141,218,233,244,270,276,288,351,402,433,437],"platelet-derived":[16],"(PDGF)":[19],"(PDGFR),":[21],"recruit":[22],"downstream":[23],"signalling":[24,112,198,344,449],"proteins,":[25],"including":[26,240],"phosphatidylinositol":[27,43,51,82],"3-kinase":[28],"(PI3K).":[29],"PI3K,":[30],"composed":[31],"of":[32,105,168,179,190,195,201,297,398,446],"a":[33,38,77,94,107,247,323],"regulatory":[34],"p85":[35,434],"subunit":[36],"catalytic":[39],"p110":[40],"subunit,":[41],"phosphorylates":[42],"4,5-bisphosphate":[44],"at":[45,84,149,153,421],"3":[47,86],"position":[48],"to":[49,101,114,147,158,161,170,175,185,208,235,237,254,274,283,285,293,305,322,332,429,431,442,453],"generate":[50],"3,4,5-trisphosphate.":[52],"This":[53,326],"lipid":[54,163],"second":[55],"messenger":[56],"activates":[57],"Akt,":[58],"which":[59],"promotes":[60],"cell":[61,63,71],"growth,":[62],"cycle":[64],"entry":[65],"progression,":[67],"well":[69],"survival":[72],"cellular":[74,238],"migration.":[75],"PTEN,":[76,241,275,348],"tumor":[78],"suppressor":[79],"protein,":[80],"dephosphorylates":[81],"3,4,5-trisphosphate":[83],"position,":[87],"turning":[88],"off":[89],"Akt":[90,181,197,343],"signalling.":[91,182],"PTEN":[92,130,140,157,169,357,432],"contains":[93],"C-terminal":[95],"PDZ":[96,295],"binding":[97,167],"motif":[98],"that":[99,110,122,267,405],"binds":[100],"PDZ2":[103,269],"domain":[104],"MAGI-2,":[106,139,217],"scaffolding":[108,204],"protein":[109,205],"localizes":[111],"molecules":[113],"plasma":[116,151],"membrane.":[117],"MAGI-2":[118,171,203,268,278,298,318,335,353,377,425],"has":[119,133,172,426,439],"ten":[120],"domains":[121,272,280,296],"potentially":[123],"mediate":[124],"multiple":[125],"protein-protein":[126,307],"interactions":[127,211,308,394,418],"simultaneously.":[128],"A":[129],"associated-complex":[131],"(PAC)":[132],"been":[134,173,427],"described":[135],"may":[137],"contain":[138],"p85.":[142,286],"The":[143,251],"PAC":[144,192],"is":[145],"hypothesized":[146],"form":[148],"membrane":[152],"appropriate":[154],"sites":[155],"for":[156,392],"gain":[159],"access":[160],"its":[162,219],"substrates,":[164],"since":[165],"shown":[174,282,428],"enhance":[176],"suppression":[178],"PI3K-mediated":[180],"In":[183,262,303],"order":[184,304],"better":[186],"understand":[187],"role":[189],"in":[193,214,309,329,341,435,451],"attenuation":[194,445],"pathway,":[199,345],"regions":[200],"were":[206,222,229,256,281,313],"mapped":[207],"identify":[209],"taking":[212],"place":[213],"PAC.":[216],"individual":[220],"domains,":[221],"expressed":[223,320],"GST":[225,248],"fusion":[226],"proteins.":[227],"These":[228],"immobilized":[230],"onto":[231],"beads":[232],"allowed":[234],"bind":[236,273,284,292,430],"proteins":[239,252,339],"p85,":[242,349,385],"PDGFR":[243,289,350],"EGFR":[245,287,383,455],"using":[246,258],"pull-down":[249,264],"experiment.":[250],"bound":[253],"GST-MAGI-2":[255],"identified":[257],"an":[259],"immunoblot":[260],"analysis.":[261],"vitro":[263,436],"experiments":[265,370,404],"revealed":[266],"PDZ5":[271],"both":[277],"WW":[279],"did":[290,371],"not":[291,372,379],"under":[299,386],"conditions":[301,388],"studied.":[302],"study":[306],"cells,":[310],"immunoprecipitation":[311,330],"assays":[312,331],"also":[314],"performed.":[315],"Full":[316],"length":[317],"was":[319,327,366],"tagged":[321],"Myc":[324],"epitope.":[325],"used":[328],"determine":[333],"if":[334,416],"could":[336],"co-immunoprecipitate":[337,355,380],"with":[338,356,381],"involved":[340],"EGFR.":[352],"can":[354],"upon":[358],"5":[359,396],"min":[360,397],"EGF":[361],"stimulation":[362,401,413],"however,":[363],"this":[364,374],"result":[365],"inconclusive":[367],"because":[368],"replicate":[369],"verify":[373],"initial":[375],"observation.":[376],"does":[378],"nor":[384],"tested.":[389],"We":[390],"examined":[391],"these":[393,417],"after":[395,410],"more":[403],"test":[406],"different":[407],"time":[408,423],"points":[409],"would":[414],"reveal":[415],"are":[419],"present":[420],"shorter":[422],"points.":[424],"therefore":[438],"potential":[441],"regulate":[443],"PI3K/Akt":[448],"pathway":[450],"response":[452],"activated":[454],"and/or":[456],"PDGFR.":[457]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
