{"id":"https://openalex.org/W1497034987","doi":"https://doi.org/10.1002/0471142735.im1435s104","title":"Isolation and Analysis of Mouse Microglial Cells","display_name":"Isolation and Analysis of Mouse Microglial Cells","publication_year":2014,"publication_date":"2014-02-01","ids":{"openalex":"https://openalex.org/W1497034987","doi":"https://doi.org/10.1002/0471142735.im1435s104","mag":"1497034987","pmid":"https://pubmed.ncbi.nlm.nih.gov/24510618","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3980480"},"language":"en","primary_location":{"id":"doi:10.1002/0471142735.im1435s104","doi":"https://doi.org/10.1002/0471142735.im1435s104","is_oa":false,"landing_page_url":"https://doi.org/10.1002/0471142735.im1435s104","pdf_url":null,"source":{"id":"https://openalex.org/S2764460200","display_name":"Current Protocols in Immunology","issn_l":"1934-3671","issn":["1934-3671","1934-368X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Immunology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3980480","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063451331","display_name":"Jenny A. Garcia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I45438204","display_name":"The University of Texas at San Antonio","ror":"https://ror.org/01kd65564","country_code":"US","type":"education","lineage":["https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jenny A. Garcia","raw_affiliation_strings":["The University of Texas at San Antonio  San Antonio Texas","The University of Texas at San Antonio, San Antonio, Texas#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas at San Antonio  San Antonio Texas","institution_ids":["https://openalex.org/I45438204"]},{"raw_affiliation_string":"The University of Texas at San Antonio, San Antonio, Texas#TAB#","institution_ids":["https://openalex.org/I45438204"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000732775","display_name":"Sandra M. Cardona","orcid":"https://orcid.org/0000-0001-9128-6742","observed_orcids":["https://orcid.org/0000-0001-9128-6742"]},"institutions":[{"id":"https://openalex.org/I45438204","display_name":"The University of Texas at San Antonio","ror":"https://ror.org/01kd65564","country_code":"US","type":"education","lineage":["https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sandra M. Cardona","raw_affiliation_strings":["The University of Texas at San Antonio  San Antonio Texas","The University of Texas at San Antonio, San Antonio, Texas#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas at San Antonio  San Antonio Texas","institution_ids":["https://openalex.org/I45438204"]},{"raw_affiliation_string":"The University of Texas at San Antonio, San Antonio, Texas#TAB#","institution_ids":["https://openalex.org/I45438204"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013624172","display_name":"Astrid E. Cardona","orcid":"https://orcid.org/0000-0002-5093-8078","observed_orcids":["https://orcid.org/0000-0002-5093-8078"]},"institutions":[{"id":"https://openalex.org/I45438204","display_name":"The University of Texas at San Antonio","ror":"https://ror.org/01kd65564","country_code":"US","type":"education","lineage":["https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Astrid E. Cardona","raw_affiliation_strings":["The University of Texas at San Antonio  San Antonio Texas","The University of Texas at San Antonio, San Antonio, Texas#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Texas at San Antonio  San Antonio Texas","institution_ids":["https://openalex.org/I45438204"]},{"raw_affiliation_string":"The University of Texas at San Antonio, San Antonio, Texas#TAB#","institution_ids":["https://openalex.org/I45438204"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5013624172"],"corresponding_institution_ids":["https://openalex.org/I45438204"],"apc_list":{"value":5050,"currency":"USD","value_usd":5050},"apc_paid":null,"fwci":1.3843,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.77241217,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":99},"biblio":{"volume":"104","issue":"1","first_page":"14.35.1","last_page":"14.35.15"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microglia","display_name":"microglia","score":0.9490000009536743},{"id":"https://openalex.org/keywords/mice","display_name":"mice","score":0.7360000014305115},{"id":"https://openalex.org/keywords/flow-cytometry","display_name":"flow cytometry","score":0.6880000233650208},{"id":"https://openalex.org/keywords/immunochemistry","display_name":"immunochemistry","score":0.8169999718666077},{"id":"https://openalex.org/keywords/myeloid-progenitor-cells","display_name":"myeloid progenitor cells","score":0.8119999766349792},{"id":"https://openalex.org/keywords/brain-homeostasis","display_name":"brain homeostasis","score":0.7820000052452087},{"id":"https://openalex.org/keywords/neuroinflammation","display_name":"neuroinflammation","score":0.8619999885559082},{"id":"https://openalex.org/keywords/central-nervous-system","display_name":"central nervous system","score":0.718999981880188}],"concepts":[{"id":"https://openalex.org/C2779830541","wikidata":"https://www.wikidata.org/wiki/Q1622829","display_name":"Microglia","level":3,"score":0.8583155870437622},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.6349881887435913},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.6213892698287964},{"id":"https://openalex.org/C87753298","wikidata":"https://www.wikidata.org/wiki/Q17157137","display_name":"Neuroinflammation","level":3,"score":0.6146190166473389},{"id":"https://openalex.org/C2780659158","wikidata":"https://www.wikidata.org/wiki/Q2466273","display_name":"Immunochemistry","level":3,"score":0.5864499807357788},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5675668120384216},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.5566036701202393},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5312483310699463},{"id":"https://openalex.org/C63645605","wikidata":"https://www.wikidata.org/wiki/Q103191","display_name":"Homeostasis","level":2,"score":0.49145829677581787},{"id":"https://openalex.org/C529278444","wikidata":"https://www.wikidata.org/wiki/Q47273","display_name":"Central nervous system","level":2,"score":0.43820497393608093},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.42449191212654114},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.3842995762825012},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.35729965567588806},{"id":"https://openalex.org/C2776914184","wikidata":"https://www.wikidata.org/wiki/Q101991","display_name":"Inflammation","level":2,"score":0.32102668285369873},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.24592074751853943},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.21435749530792236},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.19827434420585632}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002490","descriptor_name":"Central Nervous System","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006706","descriptor_name":"Homeostasis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007109","descriptor_name":"Immunity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008810","descriptor_name":"Mice, Inbred C57BL","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017628","descriptor_name":"Microglia","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D023461","descriptor_name":"Myeloid Progenitor Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1002/0471142735.im1435s104","doi":"https://doi.org/10.1002/0471142735.im1435s104","is_oa":false,"landing_page_url":"https://doi.org/10.1002/0471142735.im1435s104","pdf_url":null,"source":{"id":"https://openalex.org/S2764460200","display_name":"Current Protocols in Immunology","issn_l":"1934-3671","issn":["1934-3671","1934-368X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Immunology","raw_type":"journal-article"},{"id":"pmid:24510618","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24510618","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current protocols in immunology","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:3282618","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3980480","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3980480","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3980480","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Curr Protoc Immunol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:3282618","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3980480","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1279492459","display_name":"Role of CX3CR1 in adaptive immunity during autoimmune encephalomyelitis","funder_award_id":"SC1GM095426","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G2022928122","display_name":"Role of C-type Lectin Receptors in Myeloid Plasticity in Neurocysticercosis","funder_award_id":"R01NS078501","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":14,"referenced_works":["https://openalex.org/W1641259203","https://openalex.org/W1985502949","https://openalex.org/W2017756051","https://openalex.org/W2022585532","https://openalex.org/W2036742353","https://openalex.org/W2050707975","https://openalex.org/W2077003881","https://openalex.org/W2102885018","https://openalex.org/W2113107925","https://openalex.org/W2117723028","https://openalex.org/W2125281412","https://openalex.org/W2142750845","https://openalex.org/W2161057248","https://openalex.org/W2169575313"],"related_works":["https://openalex.org/W3033887425","https://openalex.org/W2286659041","https://openalex.org/W2804367279","https://openalex.org/W2569452645","https://openalex.org/W3134324835","https://openalex.org/W4234051501","https://openalex.org/W7214197034","https://openalex.org/W3200544031","https://openalex.org/W2900328090","https://openalex.org/W2104520325"],"abstract_inverted_index":{"Microglia":[0],"are":[1,16,37,101],"mononuclear":[2],"phagocytes":[3],"that":[4,67],"make":[5],"up":[6],"about":[7],"10%":[8],"of":[9,26,80],"the":[10,57],"central":[11],"nervous":[12],"system":[13],"(CNS).":[14],"They":[15],"known":[17],"for":[18,72,88],"their":[19,33],"surveillant":[20],"behavior,":[21],"which":[22],"involves":[23],"continuous":[24],"monitoring":[25],"neural":[27],"tissue":[28],"by":[29,84],"extending":[30],"and":[31,43,53,77,96],"retracting":[32],"processes.":[34],"Microglial":[35],"cells":[36,42,83,91],"derived":[38],"from":[39],"myeloid":[40],"progenitor":[41],"play":[44],"important":[45],"roles":[46],"in":[47,56,93,98],"homeostasis":[48],"as":[49,51],"well":[50],"inflammatory":[52],"immune":[54],"responses":[55],"brain.":[58],"This":[59],"unit":[60],"describes":[61],"several":[62],"microglial":[63,82,90],"cell":[64],"isolation":[65],"protocols":[66],"can":[68],"be":[69],"easily":[70],"adapted":[71],"projects":[73],"requiring":[74],"a":[75],"rapid":[76],"efficient":[78],"analysis":[79],"mouse":[81],"flow":[85],"cytometry.":[86],"Methods":[87],"visualizing":[89],"using":[92],"situ":[94],"immunohistochemistry":[95],"immunochemistry":[97],"free-floating":[99],"sections":[100],"also":[102],"included.":[103]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":10},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":5},{"year":2015,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}