{"id":"https://openalex.org/W1496566912","doi":"https://doi.org/10.1128/aem.63.8.3261-3267.1997","title":"Quantitative fluorescence in situ hybridization of Aureobasidium pullulans on microscope slides and leaf surfaces","display_name":"Quantitative fluorescence in situ hybridization of Aureobasidium pullulans on microscope slides and leaf surfaces","publication_year":1997,"publication_date":"1997-08-01","ids":{"openalex":"https://openalex.org/W1496566912","doi":"https://doi.org/10.1128/aem.63.8.3261-3267.1997","mag":"1496566912","pmid":"https://pubmed.ncbi.nlm.nih.gov/9251214","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/168625"},"language":"en","primary_location":{"id":"doi:10.1128/aem.63.8.3261-3267.1997","doi":"https://doi.org/10.1128/aem.63.8.3261-3267.1997","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.63.8.3261-3267.1997","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/168625","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026289416","display_name":"Siyu Li","orcid":"https://orcid.org/0009-0007-1674-1419","observed_orcids":["https://orcid.org/0009-0007-1674-1419"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S Li","raw_affiliation_strings":["Department of Plant Pathology, University of Wisconsin, Madison 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Pathology, University of Wisconsin, Madison 53706, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032154035","display_name":"Russell N. Spear","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R N Spear","raw_affiliation_strings":["Department of Plant Pathology, University of Wisconsin, Madison 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Pathology, University of Wisconsin, Madison 53706, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"last","author":{"id":null,"display_name":"J H Andrews","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J H Andrews","raw_affiliation_strings":["Department of Plant Pathology, University of Wisconsin, Madison 53706, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Pathology, University of Wisconsin, Madison 53706, USA","institution_ids":["https://openalex.org/I135310074"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I135310074"],"apc_list":null,"apc_paid":null,"fwci":7.0116,"has_fulltext":false,"cited_by_count":54,"citation_normalized_percentile":{"value":0.96689142,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"63","issue":"8","first_page":"3261","last_page":"3267"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.9164999723434448,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.9164999723434448,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12668","display_name":"Yeasts and Rust Fungi Studies","score":0.02459999918937683,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11900","display_name":"Fungal Plant Pathogen Control","score":0.0142000000923872,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aureobasidium-pullulans","display_name":"Aureobasidium pullulans","score":1.0},{"id":"https://openalex.org/keywords/quantitative-fluorescence-in-situ-hybridization","display_name":"quantitative fluorescence in situ hybridization","score":0.8659999966621399},{"id":"https://openalex.org/keywords/phyllosphere","display_name":"phyllosphere","score":0.7639999985694885},{"id":"https://openalex.org/keywords/epiphytic-fungi","display_name":"epiphytic fungi","score":0.7300000190734863},{"id":"https://openalex.org/keywords/apple-leaves","display_name":"apple leaves","score":0.8360000252723694},{"id":"https://openalex.org/keywords/signal-to-noise-ratio","display_name":"signal-to-noise ratio","score":0.7789999842643738},{"id":"https://openalex.org/keywords/cryptococcus-laurentii","display_name":"Cryptococcus laurentii","score":0.796999990940094}],"concepts":[{"id":"https://openalex.org/C2779454560","wikidata":"https://www.wikidata.org/wiki/Q147300","display_name":"Aureobasidium pullulans","level":3,"score":0.8794796466827393},{"id":"https://openalex.org/C205626393","wikidata":"https://www.wikidata.org/wiki/Q2089263","display_name":"Phyllosphere","level":3,"score":0.6752948760986328},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6068247556686401},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.557852566242218},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5520240068435669},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.5172929763793945},{"id":"https://openalex.org/C2777542201","wikidata":"https://www.wikidata.org/wiki/Q1336182","display_name":"Fluorescence in situ hybridization","level":4,"score":0.5132946372032166},{"id":"https://openalex.org/C179687394","wikidata":"https://www.wikidata.org/wiki/Q4826281","display_name":"Autofluorescence","level":3,"score":0.46211934089660645},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.1774553656578064},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.16611573100090027},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15959885716438293},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11055195331573486},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0},{"id":"https://openalex.org/C30481170","wikidata":"https://www.wikidata.org/wiki/Q37748","display_name":"Chromosome","level":3,"score":0.0},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.0},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000898","descriptor_name":"Antibiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003593","descriptor_name":"Cytoplasm","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D003904","descriptor_name":"Mitosporic Fungi","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003904","descriptor_name":"Mitosporic Fungi","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D005658","descriptor_name":"Fungi","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005658","descriptor_name":"Fungi","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012337","descriptor_name":"RNA, Ribosomal, 18S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014197","descriptor_name":"Trees","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D015169","descriptor_name":"Colony Count, Microbial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015345","descriptor_name":"Oligonucleotide Probes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017404","descriptor_name":"In Situ Hybridization, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/aem.63.8.3261-3267.1997","doi":"https://doi.org/10.1128/aem.63.8.3261-3267.1997","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.63.8.3261-3267.1997","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:197521","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/168625","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:9251214","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9251214","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:168625","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC168625/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:197521","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/168625","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W70588581","https://openalex.org/W74255643","https://openalex.org/W207291951","https://openalex.org/W302119829","https://openalex.org/W1516283466","https://openalex.org/W1528392499","https://openalex.org/W1533811233","https://openalex.org/W1549149232","https://openalex.org/W1561580658","https://openalex.org/W1592033651","https://openalex.org/W1599599403","https://openalex.org/W1601798363","https://openalex.org/W1606920154","https://openalex.org/W1701869895","https://openalex.org/W1770972723","https://openalex.org/W1837751427","https://openalex.org/W1848498826","https://openalex.org/W1964125949","https://openalex.org/W1985039062","https://openalex.org/W1991713860","https://openalex.org/W1996540586","https://openalex.org/W2004172091","https://openalex.org/W2004579488","https://openalex.org/W2013537366","https://openalex.org/W2017627072","https://openalex.org/W2022298315","https://openalex.org/W2026277470","https://openalex.org/W2035775871","https://openalex.org/W2047295844","https://openalex.org/W2049016932","https://openalex.org/W2059798890","https://openalex.org/W2068381544","https://openalex.org/W2088527065","https://openalex.org/W2099073787","https://openalex.org/W2120772790","https://openalex.org/W2127334371","https://openalex.org/W2131988453","https://openalex.org/W2139555954","https://openalex.org/W2173785122","https://openalex.org/W2174222776","https://openalex.org/W2223898215","https://openalex.org/W2549770557"],"related_works":["https://openalex.org/W2002969446","https://openalex.org/W2139555954","https://openalex.org/W7027733572","https://openalex.org/W2117721499","https://openalex.org/W2247774203","https://openalex.org/W1985034191","https://openalex.org/W2084757839","https://openalex.org/W2741840743","https://openalex.org/W1976965773","https://openalex.org/W7217739631"],"abstract_inverted_index":{"A":[0],"21-mer":[1],"oligonucleotide":[2],"probe":[3,58],"designated":[4],"Ap665,":[5],"directed":[6],"at":[7,80,167],"the":[8,33,60,66,102,106,149,152,208,217,225,230,238,263],"18S":[9],"rRNA":[10],"of":[11,19,32,151,178,184,207,219,240],"Aureobasidium":[12],"pullulans":[13,77,162,198,260],"and":[14,37,44,53,59,109,130,134,145,216,245,257],"labelled":[15,56],"with":[16,74,123,160,180],"five":[17],"molecules":[18],"fluorescein":[20],"isothiocyanate,":[21],"was":[22,69,97,138],"applied":[23],"by":[24,117,222,237],"fluorescence":[25,103,143,153,174],"in":[26,155,176,262],"situ":[27],"hybridization":[28,67,228],"(FISH)":[29],"to":[30,99,110,140,146,194,255],"populations":[31,199],"fungus":[34],"on":[35,114,201],"slides":[36],"apple":[38],"leaves":[39,223],"from":[40,105,172],"growth":[41],"chamber":[42],"seedlings":[43],"orchard":[45],"trees.":[46],"In":[47],"specificity":[48],"tests":[49],"that":[50,101,250],"included":[51],"Ap665":[52,72,159,179],"a":[54,124,168],"similarly":[55],"universal":[57],"respective":[61],"complementary":[62],"probes":[63],"as":[64],"controls,":[65],"signal":[68,154],"strong":[70],"for":[71,85,132,227],"reactions":[73],"12":[75],"A.":[76,161,197,259],"strains":[78],"but":[79],"or":[81,204],"below":[82],"background":[83],"level":[84,171],"98":[86],"other":[87],"fungi":[88],"including":[89],"82":[90],"phylloplane":[91],"isolates.":[92],"Scanning":[93],"confocal":[94],"laser":[95],"microscopy":[96,116],"used":[98,139,192,254],"confirm":[100],"originated":[104],"cytoplasmic":[107],"matrix":[108],"overcome":[111],"limitations":[112],"imposed":[113],"conventional":[115],"leaf":[118,209,214],"topography.":[119],"Images":[120],"were":[121,191,235],"recorded":[122],"cooled":[125],"charge-coupled":[126],"device":[127],"video":[128],"camera":[129],"digitized":[131],"storage":[133],"manipulation.":[135],"Image":[136,188],"analysis":[137,189],"verify":[141],"semiquantitative":[142],"ratings":[144],"demonstrate":[147],"how":[148],"distribution":[150],"specific":[156],"interactions":[157,177],"(e.g.,":[158,175],"cells)":[163,183],"could":[164],"be":[165,253],"separated":[166],"given":[169],"probability":[170],"nonspecific":[173],"Cryptococcus":[181],"laurentii":[182],"an":[185],"overlapping":[186],"population.":[187],"methods":[190],"also":[193],"quantify":[195,258],"epiphytic":[196],"based":[200],"cell":[202],"number":[203],"percent":[205],"coverage":[206],"surface.":[210],"Under":[211],"some":[212],"conditions,":[213],"autofluorescence":[215],"release":[218],"fluorescent":[220],"compounds":[221],"during":[224],"processing":[226],"decreased":[229],"signal-to-noise":[231],"ratio.":[232],"These":[233],"effects":[234],"reduced":[236],"use":[239],"appropriate":[241],"excitation":[242],"filter":[243],"sets":[244],"fixation":[246],"conditions.":[247],"We":[248],"conclude":[249],"FISH":[251],"can":[252],"detect":[256],"cells":[261],"phyllosphere.":[264]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}