{"id":"https://openalex.org/W1496082479","doi":"https://doi.org/10.1016/s0021-9258(19)75219-3","title":"Acanthamoeba cofactor protein is a heavy chain kinase required for actin activation of the Mg2+-ATPase activity of Acanthamoeba myosin I","display_name":"Acanthamoeba cofactor protein is a heavy chain kinase required for actin activation of the Mg2+-ATPase activity of Acanthamoeba myosin I","publication_year":1977,"publication_date":"1977-12-01","ids":{"openalex":"https://openalex.org/W1496082479","doi":"https://doi.org/10.1016/s0021-9258(19)75219-3","mag":"1496082479","pmid":"https://pubmed.ncbi.nlm.nih.gov/144730"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)75219-3","doi":"https://doi.org/10.1016/s0021-9258(19)75219-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)75219-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)75219-3","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101548568","display_name":"Hiroshi Maruta","orcid":"https://orcid.org/0000-0002-9087-3387","observed_orcids":["https://orcid.org/0000-0002-9087-3387"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H. Maruta","raw_affiliation_strings":["From the Laboratory of Cell Biology, National Heart, Lung, and Blood Institute,","National Znstitutes ofHealth, Bethesda, Maryland 20014"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Laboratory of Cell Biology, National Heart, Lung, and Blood Institute,","institution_ids":["https://openalex.org/I4210106489"]},{"raw_affiliation_string":"National Znstitutes ofHealth, Bethesda, Maryland 20014","institution_ids":["https://openalex.org/I1299303238"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056320284","display_name":"Edward D. Korn","orcid":"https://orcid.org/0000-0003-3867-6295","observed_orcids":["https://orcid.org/0000-0003-3867-6295"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"E.D. Korn","raw_affiliation_strings":["From the Laboratory of Cell Biology, National Heart, Lung, and Blood Institute,","National Znstitutes ofHealth, Bethesda, Maryland 20014"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Laboratory of Cell Biology, National Heart, Lung, and Blood Institute,","institution_ids":["https://openalex.org/I4210106489"]},{"raw_affiliation_string":"National Znstitutes ofHealth, Bethesda, Maryland 20014","institution_ids":["https://openalex.org/I1299303238"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":7.8248,"has_fulltext":false,"cited_by_count":126,"citation_normalized_percentile":{"value":0.97164757,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"252","issue":"23","first_page":"8329","last_page":"8332"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10882","display_name":"Cardiomyopathy and Myosin Studies","score":0.5224999785423279,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10882","display_name":"Cardiomyopathy and Myosin Studies","score":0.5224999785423279,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.17630000412464142,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.12290000170469284,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/actin-activation","display_name":"actin activation","score":0.8569999933242798},{"id":"https://openalex.org/keywords/mg2-atpase-activity","display_name":"Mg2+-ATPase activity","score":0.9490000009536743},{"id":"https://openalex.org/keywords/actin-filaments","display_name":"F-actin","score":0.8339999914169312},{"id":"https://openalex.org/keywords/myosin-light-chain","display_name":"myosin light chain","score":0.6940000057220459},{"id":"https://openalex.org/keywords/acanthamoeba","display_name":"Acanthamoeba","score":0.6740000247955322}],"concepts":[{"id":"https://openalex.org/C8035138","wikidata":"https://www.wikidata.org/wiki/Q6017854","display_name":"Myosin light-chain kinase","level":3,"score":0.8151087760925293},{"id":"https://openalex.org/C6997183","wikidata":"https://www.wikidata.org/wiki/Q271727","display_name":"Myosin","level":2,"score":0.8054889440536499},{"id":"https://openalex.org/C2778868110","wikidata":"https://www.wikidata.org/wiki/Q1152218","display_name":"Acanthamoeba","level":2,"score":0.7574771642684937},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.6868340373039246},{"id":"https://openalex.org/C181912034","wikidata":"https://www.wikidata.org/wiki/Q1101143","display_name":"Dephosphorylation","level":4,"score":0.6140430569648743},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.5941796898841858},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.584587574005127},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5414036512374878},{"id":"https://openalex.org/C23265538","wikidata":"https://www.wikidata.org/wiki/Q300033","display_name":"ATPase","level":3,"score":0.5025415420532227},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.44185879826545715},{"id":"https://openalex.org/C178666793","wikidata":"https://www.wikidata.org/wiki/Q422476","display_name":"Phosphatase","level":3,"score":0.4389202296733856},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.38389548659324646},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.25434452295303345},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.05798354744911194}],"mesh":[{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000251","descriptor_name":"Adenosine Triphosphatases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000656","descriptor_name":"Amoeba","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008274","descriptor_name":"Magnesium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009218","descriptor_name":"Myosins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D009218","descriptor_name":"Myosins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)75219-3","doi":"https://doi.org/10.1016/s0021-9258(19)75219-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)75219-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:144730","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/144730","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)75219-3","doi":"https://doi.org/10.1016/s0021-9258(19)75219-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)75219-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":17,"referenced_works":["https://openalex.org/W1508180802","https://openalex.org/W1548538265","https://openalex.org/W1549082848","https://openalex.org/W1556587164","https://openalex.org/W1651271817","https://openalex.org/W1775749144","https://openalex.org/W1970793486","https://openalex.org/W1999888222","https://openalex.org/W2002681855","https://openalex.org/W2009982412","https://openalex.org/W2023245729","https://openalex.org/W2042612892","https://openalex.org/W2057353555","https://openalex.org/W2071605920","https://openalex.org/W2073879684","https://openalex.org/W2077027195","https://openalex.org/W2100837269"],"related_works":["https://openalex.org/W1501069283","https://openalex.org/W1523935457","https://openalex.org/W1485407235","https://openalex.org/W190045702","https://openalex.org/W1606723080","https://openalex.org/W1582311917","https://openalex.org/W1508180802","https://openalex.org/W2098440040","https://openalex.org/W1552235801","https://openalex.org/W1545414317"],"abstract_inverted_index":{"We":[0],"have":[1,115],"purified":[2,36,68],"a":[3,40],"cofactor":[4,37,69,95,132],"protein":[5,38,70,133],"previously":[6],"shown":[7],"(Pollard,":[8],"T.":[9],"D.,":[10],"and":[11,73,113,121,142],"Korn,":[12],"E.":[13],"D.":[14],"(1973)":[15],"J.":[16],"Biol.":[17],"Chem.":[18],"248,":[19],"4691-4697)":[20],"to":[21],"be":[22],"required":[23,153],"for":[24,154],"actin":[25,155],"activation":[26,156],"of":[27,31,52,57,62,81,94,124,145,149,157],"the":[28,46,53,67,92,119,146],"Mg2+-ATPase":[29,79,98,159],"activity":[30,80,99],"Acanthamoeba":[32,58,63,83,104,125,136],"myosin":[33,42,59,64,84,105,126,137,151],"I.":[34,60,127],"The":[35,78],"is":[39,76,86,100,107,134,152],"novel":[41],"kinase":[43,141],"that":[44,131,143],"phosphorylates":[45],"single":[47],"heavy":[48,139,147],"chain,":[49],"but":[50,75],"neither":[51],"two":[54],"light":[55],"chains,":[56],"Phosphorylation":[61,112],"I":[65,85,106,138],"by":[66,89,109],"requires":[71],"ATP":[72],"Mg2+":[74],"Ca2+-independent.":[77],"phosphorylated":[82,103],"highly":[87],"activated":[88],"F-actin":[90],"in":[91],"absence":[93],"protein.":[96],"Actin-activated":[97],"lost":[101],"when":[102],"dephosphorylated":[108],"platelet":[110],"phosphatase.":[111],"dephosphorylation":[114],"no":[116],"effect":[117],"on":[118],"(K+,EDTA)-ATPase":[120],"Ca2+-ATPase":[122],"activities":[123],"These":[128],"results":[129],"show":[130],"an":[135],"chain":[140,148],"phosphorylation":[144],"this":[150],"its":[158],"activity.":[160]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}