{"id":"https://openalex.org/W1495814740","doi":"https://doi.org/10.1016/s0021-9258(19)85088-3","title":"Identification and in Vitro Reconstitution of Lysosomal Neuraminidase from Human Placenta","display_name":"Identification and in Vitro Reconstitution of Lysosomal Neuraminidase from Human Placenta","publication_year":1989,"publication_date":"1989-01-01","ids":{"openalex":"https://openalex.org/W1495814740","doi":"https://doi.org/10.1016/s0021-9258(19)85088-3","mag":"1495814740","pmid":"https://pubmed.ncbi.nlm.nih.gov/2492018"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)85088-3","doi":"https://doi.org/10.1016/s0021-9258(19)85088-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)85088-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)85088-3","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087343763","display_name":"Gijsbertus T. J. van der Horst","orcid":"https://orcid.org/0000-0002-7773-6396","observed_orcids":["https://orcid.org/0000-0002-7773-6396"]},"institutions":[{"id":"https://openalex.org/I913958620","display_name":"Erasmus University Rotterdam","ror":"https://ror.org/057w15z03","country_code":"NL","type":"education","lineage":["https://openalex.org/I913958620"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"G T J van der Horst","raw_affiliation_strings":["Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I913958620"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068993400","display_name":"Niels Galjart","orcid":"https://orcid.org/0000-0003-1964-3607","observed_orcids":["https://orcid.org/0000-0003-1964-3607"]},"institutions":[{"id":"https://openalex.org/I913958620","display_name":"Erasmus University Rotterdam","ror":"https://ror.org/057w15z03","country_code":"NL","type":"education","lineage":["https://openalex.org/I913958620"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"N J Galjart","raw_affiliation_strings":["Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I913958620"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074222588","display_name":"Alessandra d\u2019Azzo","orcid":"https://orcid.org/0000-0003-1747-7502","observed_orcids":["https://orcid.org/0000-0003-1747-7502"]},"institutions":[{"id":"https://openalex.org/I913958620","display_name":"Erasmus University Rotterdam","ror":"https://ror.org/057w15z03","country_code":"NL","type":"education","lineage":["https://openalex.org/I913958620"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"A d'Azzo","raw_affiliation_strings":["Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I913958620"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5106668236","display_name":"Hans GALJAARD","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I913958620","display_name":"Erasmus University Rotterdam","ror":"https://ror.org/057w15z03","country_code":"NL","type":"education","lineage":["https://openalex.org/I913958620"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"H Galjaard","raw_affiliation_strings":["Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I913958620"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005464442","display_name":"F.W. Verheijen","orcid":"https://orcid.org/0000-0002-5995-3469","observed_orcids":["https://orcid.org/0000-0002-5995-3469"]},"institutions":[{"id":"https://openalex.org/I913958620","display_name":"Erasmus University Rotterdam","ror":"https://ror.org/057w15z03","country_code":"NL","type":"education","lineage":["https://openalex.org/I913958620"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"F W Verheijen","raw_affiliation_strings":["Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Cell Biology and Genetics, Erasmus University Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I913958620"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I913958620"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.0152,"has_fulltext":true,"cited_by_count":98,"citation_normalized_percentile":{"value":0.93110778,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"264","issue":"2","first_page":"1317","last_page":"1322"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9570000171661377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9570000171661377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10945","display_name":"Lysosomal Storage Disorders Research","score":0.03400000184774399,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics (clinical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-placenta","display_name":"human placenta","score":0.9750000238418579},{"id":"https://openalex.org/keywords/beta-galactosidase","display_name":"beta-galactosidase","score":0.9459999799728394},{"id":"https://openalex.org/keywords/protective-proteins","display_name":"protective proteins","score":0.9300000071525574},{"id":"https://openalex.org/keywords/enzyme-reconstitution","display_name":"enzyme reconstitution","score":0.656000018119812},{"id":"https://openalex.org/keywords/n-glycosylation","display_name":"N-glycosylation","score":0.7300000190734863},{"id":"https://openalex.org/keywords/glycoprotein-complex","display_name":"glycoprotein complex","score":0.6050000190734863},{"id":"https://openalex.org/keywords/enzyme-stability","display_name":"enzyme stability","score":0.5270000100135803}],"concepts":[{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.776373028755188},{"id":"https://openalex.org/C134164806","wikidata":"https://www.wikidata.org/wiki/Q409485","display_name":"Neuraminidase","level":3,"score":0.7716355323791504},{"id":"https://openalex.org/C2776953305","wikidata":"https://www.wikidata.org/wiki/Q1212935","display_name":"Placenta","level":4,"score":0.6821042895317078},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.5834358334541321},{"id":"https://openalex.org/C3018059006","wikidata":"https://www.wikidata.org/wiki/Q1212935","display_name":"Human placenta","level":5,"score":0.4966960549354553},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4476875960826874},{"id":"https://openalex.org/C2780216420","wikidata":"https://www.wikidata.org/wiki/Q83330","display_name":"Lysosome","level":3,"score":0.4380944073200226},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.400936484336853},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3676045536994934},{"id":"https://openalex.org/C172680121","wikidata":"https://www.wikidata.org/wiki/Q26513","display_name":"Fetus","level":3,"score":0.2223474085330963},{"id":"https://openalex.org/C2779234561","wikidata":"https://www.wikidata.org/wiki/Q11995","display_name":"Pregnancy","level":2,"score":0.205358624458313},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1770033836364746},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001616","descriptor_name":"beta-Galactosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008247","descriptor_name":"Lysosomes","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009439","descriptor_name":"Neuraminidase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D009439","descriptor_name":"Neuraminidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010920","descriptor_name":"Placenta","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D011247","descriptor_name":"Pregnancy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0021-9258(19)85088-3","doi":"https://doi.org/10.1016/s0021-9258(19)85088-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)85088-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:2492018","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2492018","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pure.eur.nl:openaire/e2e986ae-0d82-4434-b371-924a32d46768","doi":null,"is_oa":true,"landing_page_url":"https://www.scopus.com/pages/publications/0024495357","pdf_url":null,"source":{"id":"https://openalex.org/S4306401266","display_name":"EUR Research Repository (Erasmus University Rotterdam)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I913958620","host_organization_name":"Erasmus University Rotterdam","host_organization_lineage":["https://openalex.org/I913958620"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Van der Horst, G T J, Galjart, N J, D'Azzo, A, Galjaard, H & Verheijen, F W 1989, 'Identification and in vitro reconstitution of lysosomal neuraminidase from human placenta', Journal of Biological Chemistry, vol. 264, no. 2, pp. 1317-1322.","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)85088-3","doi":"https://doi.org/10.1016/s0021-9258(19)85088-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)85088-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.6240000128746033,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W139082413","https://openalex.org/W585721197","https://openalex.org/W651526554","https://openalex.org/W1480165684","https://openalex.org/W1495761998","https://openalex.org/W1549486981","https://openalex.org/W1601276957","https://openalex.org/W1604233325","https://openalex.org/W1624368848","https://openalex.org/W1775749144","https://openalex.org/W1826433977","https://openalex.org/W1965476361","https://openalex.org/W1977623329","https://openalex.org/W1995701455","https://openalex.org/W2009123990","https://openalex.org/W2009307508","https://openalex.org/W2009734585","https://openalex.org/W2024118722","https://openalex.org/W2025661319","https://openalex.org/W2066127869","https://openalex.org/W2075493559","https://openalex.org/W2075792282","https://openalex.org/W2098248666","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2131424376","https://openalex.org/W2167052275","https://openalex.org/W2395417935"],"related_works":["https://openalex.org/W2066127869","https://openalex.org/W1601276957","https://openalex.org/W1872184364","https://openalex.org/W2124325580","https://openalex.org/W1016546238","https://openalex.org/W1982328690","https://openalex.org/W2089241378","https://openalex.org/W1984255879","https://openalex.org/W2159623646","https://openalex.org/W2419343687"],"abstract_inverted_index":{"Lysosomal":[0],"neuraminidase":[1,17,39,56,85,94,114,142,147,155],"from":[2,87],"human":[3],"placenta":[4],"has":[5],"been":[6],"obtained":[7],"in":[8,48,123],"its":[9,49],"active":[10,110],"form":[11],"by":[12],"association":[13,158],"of":[14,75,82,93,97,101,109,113,126,136,145],"an":[15],"inactive":[16,38],"polypeptide":[18,60,86,148],"with":[19,55,67,159],"beta-galactosidase":[20,98,160],"and":[21,52,149],"the":[22,37,83,91,102,107,127,137,146,150],"protective":[23,103,151],"protein.":[24],"Using":[25],"a":[26,33,72],"specific":[27],"antiserum,":[28],"we":[29],"have":[30],"now":[31],"identified":[32],"66-kDa":[34,59,84],"protein":[35,65,104,129,152],"as":[36],"polypeptide.":[40],"It":[41],"is":[42,61,116,161],"specifically":[43],"recognized":[44],"on":[45],"immunoblots":[46],"only":[47],"nonreduced":[50],"state,":[51],"it":[53],"coprecipitates":[54],"activity.":[57,95,143],"The":[58],"substantially":[62],"glycosylated":[63],"(38-kDa":[64],"core":[66],"7-14":[68],"N-linked":[69],"oligosaccharide":[70],"chains),":[71],"feature":[73],"characteristic":[74],"lysosomal":[76],"integral":[77],"membrane":[78],"proteins.":[79],"Specific":[80],"removal":[81],"glycoprotein":[88,120],"preparations":[89],"prevents":[90],"generation":[92],"Removal":[96],"or":[99],"destruction":[100],"also":[105],"hinders":[106],"formation":[108],"neuraminidase.":[111],"Reconstitution":[112],"activity":[115],"observed":[117],"after":[118],"mixing":[119],"preparations,":[121],"depleted":[122],"different":[124],"components":[125,135],"beta-galactosidase-neuraminidase-protective":[128],"complex,":[130],"indicating":[131],"that":[132],"all":[133],"three":[134],"complex":[138],"are":[139],"required":[140,162],"for":[141,163],"Association":[144],"generates":[153],"unstable":[154],"activity,":[156],"whereas":[157],"stability.":[164]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}