{"id":"https://openalex.org/W1491579474","doi":null,"title":"Detection of CD4 T-cell responses to a tumor vaccine by cytokine flow cytometry.","display_name":"Detection of CD4 T-cell responses to a tumor vaccine by cytokine flow cytometry.","publication_year":2001,"publication_date":"2001-03-01","ids":{"openalex":"https://openalex.org/W1491579474","mag":"1491579474","pmid":"https://pubmed.ncbi.nlm.nih.gov/11300490"},"language":"en","primary_location":{"id":"pmid:11300490","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11300490","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical cancer research : an official journal of the American Association for Cancer Research","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068399737","display_name":"Holden Terry Maecker","orcid":"https://orcid.org/0000-0003-0795-9946","observed_orcids":["https://orcid.org/0000-0003-0795-9946","https://orcid.org/0000-0001-5389-0906"]},"institutions":[{"id":"https://openalex.org/I4210116987","display_name":"BD Biosciences (United States)","ror":"https://ror.org/02h9td068","country_code":"US","type":"company","lineage":["https://openalex.org/I146461966","https://openalex.org/I4210116987"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"H T Maecker","raw_affiliation_strings":["BD Biosciences, Immunocytometry Systems, San Jose, California 95131, USA. holden_maecker@bdis.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BD Biosciences, Immunocytometry Systems, San Jose, California 95131, USA. holden_maecker@bdis.com","institution_ids":["https://openalex.org/I4210116987"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089342900","display_name":"Susanne Auffermann-Gretzinger","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Auffermann-Gretzinger","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091834453","display_name":"Laurel E. Nomura","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L E Nomura","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043881228","display_name":"Arcangelo Liso","orcid":"https://orcid.org/0000-0001-5638-227X","observed_orcids":["https://orcid.org/0000-0001-5638-227X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Liso","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110968248","display_name":"Debra Katherine Czerwinski","orcid":"https://orcid.org/0009-0006-8415-1632","observed_orcids":["https://orcid.org/0009-0006-8415-1632"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D K Czerwinski","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5083342021","display_name":"Ronald Levy","orcid":"https://orcid.org/0000-0003-2061-0650","observed_orcids":["https://orcid.org/0000-0003-2061-0650"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R Levy","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.1556,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.87166579,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"7","issue":"3 Suppl","first_page":"902s","last_page":"908s"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cytokine-flow-cytometry","display_name":"cytokine flow cytometry","score":0.9900000095367432},{"id":"https://openalex.org/keywords/cd4-t-cell-responses","display_name":"CD4+ T cell responses","score":0.9549999833106995},{"id":"https://openalex.org/keywords/cancer-vaccines","display_name":"cancer vaccines","score":0.6660000085830688},{"id":"https://openalex.org/keywords/multiple-myeloma","display_name":"multiple myeloma","score":0.8679999709129333},{"id":"https://openalex.org/keywords/dendritic-cell-vaccination","display_name":"dendritic cell vaccination","score":0.972000002861023},{"id":"https://openalex.org/keywords/intracellular-cytokine-staining","display_name":"intracellular cytokine staining","score":0.8259999752044678},{"id":"https://openalex.org/keywords/tnf-alpha","display_name":"TNF-\u03b1","score":0.9279999732971191},{"id":"https://openalex.org/keywords/interferon-gamma","display_name":"interferon-gamma","score":0.9679999947547913},{"id":"https://openalex.org/keywords/cd69","display_name":"CD69","score":0.8479999899864197},{"id":"https://openalex.org/keywords/keyhole-limpet-hemocyanin","display_name":"keyhole limpet hemocyanin","score":0.9739999771118164},{"id":"https://openalex.org/keywords/t-cell-proliferation","display_name":"T cell proliferation","score":0.7630000114440918},{"id":"https://openalex.org/keywords/cancer-immunotherapy","display_name":"cancer immunotherapy","score":0.7720000147819519}],"concepts":[{"id":"https://openalex.org/C2778690821","wikidata":"https://www.wikidata.org/wiki/Q212354","display_name":"Cytokine","level":2,"score":0.6510323286056519},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.6311264038085938},{"id":"https://openalex.org/C2776727803","wikidata":"https://www.wikidata.org/wiki/Q1496686","display_name":"Keyhole limpet hemocyanin","level":3,"score":0.6047424674034119},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.563217282295227},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.5083081126213074},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.486640065908432},{"id":"https://openalex.org/C17991360","wikidata":"https://www.wikidata.org/wiki/Q21173843","display_name":"Tumor necrosis factor alpha","level":2,"score":0.47196921706199646},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4637039303779602},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.43229156732559204},{"id":"https://openalex.org/C74505204","wikidata":"https://www.wikidata.org/wiki/Q1047644","display_name":"Idiotype","level":4,"score":0.42615997791290283},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3575429916381836},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.3224753737449646},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.20257210731506348}],"mesh":[{"descriptor_ui":"D000099192","descriptor_name":"CD69 Antigens","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000945","descriptor_name":"Antigens, Differentiation, T-Lymphocyte","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003713","descriptor_name":"Dendritic Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007136","descriptor_name":"Immunoglobulins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007371","descriptor_name":"Interferon-gamma","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009101","descriptor_name":"Multiple Myeloma","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D009101","descriptor_name":"Multiple Myeloma","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D014409","descriptor_name":"Tumor Necrosis Factor-alpha","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015496","descriptor_name":"CD4-Positive T-Lymphocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015703","descriptor_name":"Antigens, CD","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D015703","descriptor_name":"Antigens, CD","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016207","descriptor_name":"Cytokines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018106","descriptor_name":"CD28 Antigens","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019496","descriptor_name":"Cancer Vaccines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D037181","descriptor_name":"Lectins, C-Type","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D039441","descriptor_name":"Integrin alpha4","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:11300490","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11300490","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical cancer research : an official journal of the American Association for Cancer Research","raw_type":"Journal Article"},{"id":"mag:1491579474","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11300490","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9922999739646912}],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000879020","display_name":"Bone Marrow Grafting and Cellular Therapy for Leukemia and Lymphoma","funder_award_id":"P01CA049605","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W4752647","https://openalex.org/W83009012","https://openalex.org/W156059131","https://openalex.org/W177504901","https://openalex.org/W310472318","https://openalex.org/W1521600055","https://openalex.org/W1556003072","https://openalex.org/W1574561647","https://openalex.org/W1977595326","https://openalex.org/W1978070009","https://openalex.org/W1989052512","https://openalex.org/W1997240636","https://openalex.org/W1999002546","https://openalex.org/W2014993834","https://openalex.org/W2021488008","https://openalex.org/W2030226186","https://openalex.org/W2030769303","https://openalex.org/W2051510586","https://openalex.org/W2060303482","https://openalex.org/W2063205690","https://openalex.org/W2067069532","https://openalex.org/W2067489645","https://openalex.org/W2068819666","https://openalex.org/W2079251499","https://openalex.org/W2083947604","https://openalex.org/W2084934410","https://openalex.org/W2086704878","https://openalex.org/W2088661664","https://openalex.org/W2132512572","https://openalex.org/W2153293687","https://openalex.org/W2155782151","https://openalex.org/W2162294752","https://openalex.org/W2337393898"],"related_works":["https://openalex.org/W2132512572","https://openalex.org/W2573720116","https://openalex.org/W4317394697","https://openalex.org/W2809955431","https://openalex.org/W142768761","https://openalex.org/W2110063183","https://openalex.org/W2085508678","https://openalex.org/W1997448976","https://openalex.org/W4362672088","https://openalex.org/W3030733012"],"abstract_inverted_index":{"Cytokine":[0],"flow":[1,119],"cytometry":[2,120],"(CFC)":[3],"is":[4,213],"a":[5,127,191,218],"simple":[6,219],"and":[7,57,60,62,84,96,101,113,121,168,177,208,226],"powerful":[8],"method":[9,24],"for":[10,103],"measuring":[11],"antigen-specific":[12],"T-cell":[13,30,224],"responses":[14,31,140,171,199,202],"by":[15,118],"detection":[16,27],"of":[17,28,79,129,152,197,223],"intracellular":[18,104],"cytokine":[19,198],"staining.":[20],"We":[21],"applied":[22],"this":[23],"to":[25,32,82,141,157,164,203,229],"the":[26,77,160],"CD4":[29,130],"tumor":[33,44,142,166],"vaccines.":[34,231],"Patients":[35],"with":[36,74,91,174,187,200,210],"multiple":[37],"myeloma":[38],"were":[39,54,65,94,98,116,172],"immunized":[40],"against":[41],"their":[42],"autologous":[43],"immunoglobulin":[45,143,167],"idiotype,":[46],"using":[47],"antigen-pulsed":[48],"dendritic":[49],"cell":[50],"vaccination.":[51,150],"Blood":[52],"samples":[53],"drawn":[55],"before":[56],"after":[58,149],"vaccination,":[59],"CFC":[61,139,215],"proliferation":[63],"assays":[64],"performed.":[66],"For":[67],"CFC,":[68],"whole":[69],"blood":[70,87],"was":[71,88,184,190],"incubated":[72],"overnight":[73],"antigen":[75],"in":[76],"presence":[78],"costimulatory":[80],"antibodies":[81],"CD28":[83],"CD49d.":[85],"The":[86],"then":[89],"treated":[90],"EDTA,":[92],"erythrocytes":[93],"lysed,":[95],"leukocytes":[97],"fixed,":[99],"permeabilized,":[100],"stained":[102],"cytokines":[105],"[tumor":[106],"necrosis":[107],"factor-alpha":[108],"(TNF-alpha)":[109],"or":[110],"IFN-gamma],":[111],"CD4,":[112],"CD69.":[114],"Cells":[115],"analyzed":[117],"cytokine-producing":[122],"CD69+":[123],"cells":[124],"enumerated":[125],"as":[126],"percentage":[128],"cells.":[131],"Of":[132],"nine":[133],"patients":[134,154],"analyzed,":[135],"three":[136],"demonstrated":[137],"detectable":[138],"and/or":[144],"keyhole":[145],"limpet":[146],"hemocyanin":[147],"(KLH)":[148],"One":[151],"these":[153],"responded":[155,163],"only":[156,186],"KLH,":[158],"whereas":[159],"other":[161],"two":[162],"both":[165,175],"KLH.":[169,204],"Most":[170],"detected":[173,185],"TNF-alpha":[176],"IFN-gamma,":[178],"but":[179,193],"one":[180],"patient's":[181],"KLH":[182],"response":[183],"TNF-alpha.":[188],"There":[189],"positive,":[192],"not":[194],"strong,":[195],"correlation":[196,209],"proliferative":[201],"Although":[205],"further":[206],"follow-up":[207],"clinical":[211],"outcome":[212],"needed,":[214],"may":[216],"represent":[217],"yet":[220],"detailed":[221],"assessment":[222],"frequencies":[225],"subsets":[227],"responding":[228],"cancer":[230]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}