{"id":"https://openalex.org/W1491159633","doi":"https://doi.org/10.1046/j.1432-1327.2000.01667.x","title":"The role of Glu74 and Tyr82 in the reaction catalyzed by sheep liver cytosolic serine hydroxymethyltransferase","display_name":"The role of Glu74 and Tyr82 in the reaction catalyzed by sheep liver cytosolic serine hydroxymethyltransferase","publication_year":2000,"publication_date":"2000-10-01","ids":{"openalex":"https://openalex.org/W1491159633","doi":"https://doi.org/10.1046/j.1432-1327.2000.01667.x","mag":"1491159633","pmid":"https://pubmed.ncbi.nlm.nih.gov/10998057"},"language":"en","primary_location":{"id":"doi:10.1046/j.1432-1327.2000.01667.x","doi":"https://doi.org/10.1046/j.1432-1327.2000.01667.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1432-1327.2000.01667.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010049185","display_name":"Jala V. KRISHNA RAO","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I59270414","display_name":"Indian Institute of Science Bangalore","ror":"https://ror.org/04dese585","country_code":"IN","type":"education","lineage":["https://openalex.org/I59270414"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"J. V. Krishna Rao","raw_affiliation_strings":["Department of Biochemistry, Indian Institute of Science, Bangalore, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Indian Institute of Science, Bangalore, India","institution_ids":["https://openalex.org/I59270414"]}]},{"author_position":"middle","author":{"id":null,"display_name":"V. Prakash","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I150037166","display_name":"Central Food Technological Research Institute","ror":"https://ror.org/01d7fn555","country_code":"IN","type":"facility","lineage":["https://openalex.org/I150037166","https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"V. Prakash","raw_affiliation_strings":["Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Mysore, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Mysore, India","institution_ids":["https://openalex.org/I150037166"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113714067","display_name":"N. Appaji Rao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I59270414","display_name":"Indian Institute of Science Bangalore","ror":"https://ror.org/04dese585","country_code":"IN","type":"education","lineage":["https://openalex.org/I59270414"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"N. Appaji Rao","raw_affiliation_strings":["Department of Biochemistry, Indian Institute of Science, Bangalore, India;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Indian Institute of Science, Bangalore, India;","institution_ids":["https://openalex.org/I59270414"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113842529","display_name":"Handanhal Subbarao Savithri","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I59270414","display_name":"Indian Institute of Science Bangalore","ror":"https://ror.org/04dese585","country_code":"IN","type":"education","lineage":["https://openalex.org/I59270414"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"H. S. Savithri","raw_affiliation_strings":["Department of Biochemistry, Indian Institute of Science, Bangalore, India;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Indian Institute of Science, Bangalore, India;","institution_ids":["https://openalex.org/I59270414"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5113842529"],"corresponding_institution_ids":["https://openalex.org/I59270414"],"apc_list":null,"apc_paid":null,"fwci":1.3872,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":{"value":0.76655859,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"267","issue":"19","first_page":"5967","last_page":"5976"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.6054999828338623,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.6054999828338623,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.19020000100135803,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.13259999454021454,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/serine-hydroxymethyltransferase","display_name":"serine hydroxymethyltransferase","score":0.996999979019165},{"id":"https://openalex.org/keywords/deprotonation","display_name":"deprotonation","score":0.6489999890327454},{"id":"https://openalex.org/keywords/quinonoid-intermediate","display_name":"quinonoid intermediate","score":0.953000009059906},{"id":"https://openalex.org/keywords/hemiacetal-formation","display_name":"hemiacetal formation","score":0.8019999861717224},{"id":"https://openalex.org/keywords/reaction-specificity","display_name":"reaction specificity","score":0.47699999809265137},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.7739999890327454},{"id":"https://openalex.org/keywords/l-serine","display_name":"L-serine","score":0.6359999775886536},{"id":"https://openalex.org/keywords/enzyme-catalysis","display_name":"enzyme catalysis","score":0.31700000166893005}],"concepts":[{"id":"https://openalex.org/C2779932687","wikidata":"https://www.wikidata.org/wiki/Q24770006","display_name":"Serine hydroxymethyltransferase","level":4,"score":0.9326304793357849},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.8222736120223999},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5438322424888611},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4971065819263458},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46807894110679626},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.45595741271972656},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4455375671386719},{"id":"https://openalex.org/C68793091","wikidata":"https://www.wikidata.org/wiki/Q603891","display_name":"Transamination","level":3,"score":0.4143002927303314},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34654349088668823},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000409","descriptor_name":"Alanine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001051","descriptor_name":"Apoenzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002152","descriptor_name":"Calorimetry, Differential Scanning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003600","descriptor_name":"Cytosol","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D005557","descriptor_name":"Formaldehyde","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005557","descriptor_name":"Formaldehyde","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D011732","descriptor_name":"Pyridoxal Phosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012694","descriptor_name":"Serine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D012696","descriptor_name":"Glycine Hydroxymethyltransferase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012696","descriptor_name":"Glycine Hydroxymethyltransferase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D012756","descriptor_name":"Sheep","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013763","descriptor_name":"Tetrahydrofolates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014443","descriptor_name":"Tyrosine","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018698","descriptor_name":"Glutamic Acid","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1046/j.1432-1327.2000.01667.x","doi":"https://doi.org/10.1046/j.1432-1327.2000.01667.x","is_oa":false,"landing_page_url":"https://doi.org/10.1046/j.1432-1327.2000.01667.x","pdf_url":null,"source":{"id":"https://openalex.org/S4210214924","display_name":"European Journal of Biochemistry","issn_l":"0014-2956","issn":["0014-2956","0945-5795","1432-1033"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:10998057","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10998057","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"European journal of biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:eprints.iisc.ac.in:1808","doi":null,"is_oa":false,"landing_page_url":"http://eprints.iisc.ac.in/1808/1/33role267%2819%29-oct2000.pdf","pdf_url":"http://eprints.iisc.ac.in/1808/1/33role267%2819%29-oct2000.pdf","source":{"id":"https://openalex.org/S4377196309","display_name":"NOT FOUND REPOSITORY (Indian Institute of Science Bangalore)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I59270414","host_organization_name":"Indian Institute of Science Bangalore","host_organization_lineage":["https://openalex.org/I59270414"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"},{"id":"pmh:oai:ir.cftri.com:9343","doi":null,"is_oa":false,"landing_page_url":"http://ir.cftri.com/9343/1/Eur._J._Biochem._267%2C_5967%C2%B15976_%282000.pdf","pdf_url":"http://ir.cftri.com/9343/1/Eur._J._Biochem._267%2C_5967%C2%B15976_%282000.pdf","source":{"id":"https://openalex.org/S4406922581","display_name":"CFTRI Institutional Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W115375044","https://openalex.org/W158986363","https://openalex.org/W595325273","https://openalex.org/W938846573","https://openalex.org/W1516448862","https://openalex.org/W1533070343","https://openalex.org/W1541556471","https://openalex.org/W1860632413","https://openalex.org/W1880629566","https://openalex.org/W1968157005","https://openalex.org/W1975993749","https://openalex.org/W1977993590","https://openalex.org/W2008171507","https://openalex.org/W2029485026","https://openalex.org/W2031665112","https://openalex.org/W2035224458","https://openalex.org/W2036272330","https://openalex.org/W2049667196","https://openalex.org/W2060535373","https://openalex.org/W2087974961","https://openalex.org/W2106113567","https://openalex.org/W2128913438","https://openalex.org/W2138270253","https://openalex.org/W2143967204","https://openalex.org/W2152131459","https://openalex.org/W2208132310","https://openalex.org/W2282866563","https://openalex.org/W2314225431","https://openalex.org/W4249118749","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2143967204","https://openalex.org/W2157387803","https://openalex.org/W2087974961","https://openalex.org/W1601557355","https://openalex.org/W2103591241","https://openalex.org/W2099353611","https://openalex.org/W7071489494","https://openalex.org/W1522933483","https://openalex.org/W155157518","https://openalex.org/W2098957286"],"abstract_inverted_index":{"The":[0,97],"three-dimensional":[1],"structures":[2,157,233],"of":[3,61,121,129,150,158,171,176,183,202,234,249,253,264,273,293,305],"human":[4],"and":[5,13,18,30,83,85,108,123,160,195,236,251,276,308],"rabbit":[6],"liver":[7,66],"cytosolic":[8,67],"recombinant":[9,68],"serine":[10,39,49,69,101],"hydroxymethyltransferases":[11,50],"(hcSHMT":[12],"rcSHMT)":[14],"revealed":[15],"that":[16,139,205,214,238,284],"E75":[17,76],"Y83":[19,90],"(numbering":[20],"according":[21],"to":[22,52,57,81,95,209,256,260,278],"hcSHMT)":[23,78,92],"are":[24,44],"probable":[25],"candidates":[26],"for":[27,105,219],"proton":[28,147,307],"abstraction":[29,148,304],"Calpha-Cbeta":[31,246],"bond":[32,247],"cleavage":[33,128,248],"in":[34,47,64,77,91,145,166,199,224,242,290,302,309],"the":[35,59,103,106,119,146,167,174,179,200,220,231,267,274,291,294,303,306,311],"reaction":[36,225],"catalyzed":[37,118],"by":[38,187],"hydroxymethyltransferase.":[40],"Both":[41],"these":[42,62],"residues":[43,63],"completely":[45],"conserved":[46],"all":[48],"sequenced":[51],"date.":[53],"In":[54],"an":[55],"attempt":[56],"decipher":[58],"role":[60],"sheep":[65],"hydroxymethyltransferase":[70],"(scSHMT),":[71],"E74":[72,140,215],"(corresponding":[73,87],"residue":[74,88,255,286],"is":[75,89,287],"was":[79,93,141,163,185,207,228,240],"mutated":[80,94],"Q":[82],"K,":[84],"Y82":[86,239],"F.":[96],"specific":[98],"activities":[99],"using":[100],"as":[102,152],"substrate":[104],"E74Q":[107,172,206],"E74K":[109],"mutant":[110,116,269],"enzymes":[111,117],"were":[112],"drastically":[113],"reduced.":[114],"These":[115,211],"transamination":[120],"D-alanine":[122],"5,6,7,":[124],"8-tetrahydrofolate":[125],"independent":[126],"retroaldol":[127],"Lallo":[130],"threonine":[131],"at":[132],"rates":[133],"comparable":[134],"with":[135],"wild-type":[136],"enzyme,":[137],"suggesting":[138,283],"not":[142,288],"involved":[143,223,241,289,300],"directly":[144],"step":[149],"catalysis,":[151],"predicted":[153,229],"earlier":[154],"from":[155,230],"crystal":[156,232],"hcSHMT":[159,235],"rcSHMT.":[161],"There":[162],"no":[164],"change":[165,222],"apparent":[168,180],"Tm":[169,181],"value":[170,182],"upon":[173],"addition":[175],"L-serine,":[177],"whereas":[178],"scSHMT":[184],"enhanced":[186],"10":[188],"degrees":[189],"C.":[190],"Differential":[191],"scanning":[192],"calorimetric":[193],"data":[194],"proteolytic":[196],"digestion":[197],"patterns":[198],"presence":[201],"L-serine":[203,250],"showed":[204],"different":[208],"scSHMT.":[210],"results":[212],"indicated":[213],"might":[216,298],"be":[217,299],"required":[218],"conformational":[221],"specificity.":[226],"It":[227],"rcSHMT":[237],"hemiacetal":[243,295],"formation":[244,292],"following":[245],"mutation":[252],"this":[254,285],"F":[257],"could":[258],"lead":[259],"a":[261,280],"rapid":[262],"release":[263],"HCHO.":[265],"However,":[266],"Y82F":[268],"had":[270],"only":[271],"5%":[272],"activity":[275],"failed":[277],"form":[279],"quinonoid":[281,312],"intermediate,":[282,296],"but":[297],"indirectly":[301],"stabilizing":[310],"intermediate.":[313]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}