{"id":"https://openalex.org/W1489821100","doi":"https://doi.org/10.1002/9780470027318.a9494","title":"Single\u2010molecule Fluorescence Imaging Techniques","display_name":"Single\u2010molecule Fluorescence Imaging Techniques","publication_year":2015,"publication_date":"2015-03-12","ids":{"openalex":"https://openalex.org/W1489821100","doi":"https://doi.org/10.1002/9780470027318.a9494","mag":"1489821100"},"language":"en","primary_location":{"id":"doi:10.1002/9780470027318.a9494","doi":"https://doi.org/10.1002/9780470027318.a9494","is_oa":false,"landing_page_url":"https://doi.org/10.1002/9780470027318.a9494","pdf_url":null,"source":{"id":"https://openalex.org/S4306509318","display_name":"Encyclopedia of Analytical Chemistry","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Encyclopedia of Analytical Chemistry","raw_type":"other"},"volume":{"id":"https://openalex.org/W3035579699","display_name":"Encyclopedia of Analytical Chemistry"},"type":"reference-entry","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026920899","display_name":"Dylan A. Reid","orcid":"https://orcid.org/0000-0001-6134-1165","observed_orcids":["https://orcid.org/0000-0001-6134-1165"]},"institutions":[{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dylan A. Reid","raw_affiliation_strings":["New York University School of Medicine, New York, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New York University School of Medicine, New York, NY, USA","institution_ids":["https://openalex.org/I57206974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081602466","display_name":"Eli Rothenberg","orcid":"https://orcid.org/0000-0002-1382-1380","observed_orcids":["https://orcid.org/0000-0002-1382-1380"]},"institutions":[{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eli Rothenberg","raw_affiliation_strings":["New York University School of Medicine, New York, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New York University School of Medicine, New York, NY, USA","institution_ids":["https://openalex.org/I57206974"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I57206974"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"20"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/single-molecule-fluorescence-imaging","display_name":"single-molecule fluorescence microscopy","score":0.9559999704360962}],"concepts":[{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.661131739616394},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4348863959312439},{"id":"https://openalex.org/C57477423","wikidata":"https://www.wikidata.org/wiki/Q1434196","display_name":"Fluorescence-lifetime imaging microscopy","level":3,"score":0.4162902235984802},{"id":"https://openalex.org/C26517878","wikidata":"https://www.wikidata.org/wiki/Q228039","display_name":"Key (lock)","level":2,"score":0.41383659839630127},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.41354191303253174},{"id":"https://openalex.org/C2522767166","wikidata":"https://www.wikidata.org/wiki/Q2374463","display_name":"Data science","level":1,"score":0.36861008405685425},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.2355712652206421},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.11318814754486084},{"id":"https://openalex.org/C38652104","wikidata":"https://www.wikidata.org/wiki/Q3510521","display_name":"Computer security","level":1,"score":0.07127895951271057},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1002/9780470027318.a9494","doi":"https://doi.org/10.1002/9780470027318.a9494","is_oa":false,"landing_page_url":"https://doi.org/10.1002/9780470027318.a9494","pdf_url":null,"source":{"id":"https://openalex.org/S4306509318","display_name":"Encyclopedia of Analytical Chemistry","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Encyclopedia of Analytical Chemistry","raw_type":"other"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":217,"referenced_works":["https://openalex.org/W638709276","https://openalex.org/W793787745","https://openalex.org/W1482162053","https://openalex.org/W1489990234","https://openalex.org/W1513553433","https://openalex.org/W1521408970","https://openalex.org/W1533713273","https://openalex.org/W1643501146","https://openalex.org/W1653298906","https://openalex.org/W1672980434","https://openalex.org/W1674672657","https://openalex.org/W1675556122","https://openalex.org/W1680840469","https://openalex.org/W1964266949","https://openalex.org/W1964279141","https://openalex.org/W1965487513","https://openalex.org/W1965913747","https://openalex.org/W1966790730","https://openalex.org/W1966878060","https://openalex.org/W1969828015","https://openalex.org/W1970196890","https://openalex.org/W1970425698","https://openalex.org/W1970611074","https://openalex.org/W1972145831","https://openalex.org/W1972955366","https://openalex.org/W1973734527","https://openalex.org/W1974642741","https://openalex.org/W1975255652","https://openalex.org/W1975997119","https://openalex.org/W1976729676","https://openalex.org/W1977865284","https://openalex.org/W1979653923","https://openalex.org/W1980695770","https://openalex.org/W1980700926","https://openalex.org/W1981124682","https://openalex.org/W1981195515","https://openalex.org/W1982538875","https://openalex.org/W1983827986","https://openalex.org/W1986694578","https://openalex.org/W1987227626","https://openalex.org/W1987900501","https://openalex.org/W1988277971","https://openalex.org/W1991815226","https://openalex.org/W1992799817","https://openalex.org/W1992858116","https://openalex.org/W1994778254","https://openalex.org/W1995311028","https://openalex.org/W1998904894","https://openalex.org/W1999085806","https://openalex.org/W1999367496","https://openalex.org/W1999732173","https://openalex.org/W1999770151","https://openalex.org/W1999814206","https://openalex.org/W2000654425","https://openalex.org/W2002457457","https://openalex.org/W2004188090","https://openalex.org/W2005279566","https://openalex.org/W2005304500","https://openalex.org/W2005791001","https://openalex.org/W2006992218","https://openalex.org/W2009233209","https://openalex.org/W2009935851","https://openalex.org/W2011875417","https://openalex.org/W2014273676","https://openalex.org/W2014395811","https://openalex.org/W2015404233","https://openalex.org/W2016281621","https://openalex.org/W2016943866","https://openalex.org/W2020036966","https://openalex.org/W2020296987","https://openalex.org/W2022096765","https://openalex.org/W2022437289","https://openalex.org/W2025224752","https://openalex.org/W2026974464","https://openalex.org/W2028200156","https://openalex.org/W2028514924","https://openalex.org/W2029373146","https://openalex.org/W2031822229","https://openalex.org/W2033788106","https://openalex.org/W2034518650","https://openalex.org/W2034713721","https://openalex.org/W2034951110","https://openalex.org/W2035022037","https://openalex.org/W2036667288","https://openalex.org/W2036769067","https://openalex.org/W2036817052","https://openalex.org/W2038022359","https://openalex.org/W2041139051","https://openalex.org/W2041505869","https://openalex.org/W2043784851","https://openalex.org/W2045155739","https://openalex.org/W2047007115","https://openalex.org/W2050474263","https://openalex.org/W2051200649","https://openalex.org/W2051335807","https://openalex.org/W2051502948","https://openalex.org/W2053460598","https://openalex.org/W2054455698","https://openalex.org/W2054575184","https://openalex.org/W2055055989","https://openalex.org/W2056352332","https://openalex.org/W2056526910","https://openalex.org/W2056942342","https://openalex.org/W2057251286","https://openalex.org/W2058895666","https://openalex.org/W2060061334","https://openalex.org/W2061422980","https://openalex.org/W2063485157","https://openalex.org/W2064049346","https://openalex.org/W2064173101","https://openalex.org/W2064173118","https://openalex.org/W2065154098","https://openalex.org/W2065460329","https://openalex.org/W2066521860","https://openalex.org/W2068301995","https://openalex.org/W2069493993","https://openalex.org/W2071149713","https://openalex.org/W2072153102","https://openalex.org/W2073752458","https://openalex.org/W2074033410","https://openalex.org/W2075768046","https://openalex.org/W2075924762","https://openalex.org/W2079991169","https://openalex.org/W2081178923","https://openalex.org/W2081201480","https://openalex.org/W2081305798","https://openalex.org/W2081392226","https://openalex.org/W2082832087","https://openalex.org/W2083128912","https://openalex.org/W2083630006","https://openalex.org/W2084998277","https://openalex.org/W2085687116","https://openalex.org/W2085767196","https://openalex.org/W2085796374","https://openalex.org/W2087850846","https://openalex.org/W2088064651","https://openalex.org/W2089880432","https://openalex.org/W2092055233","https://openalex.org/W2092198587","https://openalex.org/W2094203004","https://openalex.org/W2108767279","https://openalex.org/W2110823478","https://openalex.org/W2113146347","https://openalex.org/W2113677196","https://openalex.org/W2116065010","https://openalex.org/W2117470013","https://openalex.org/W2118567895","https://openalex.org/W2119562650","https://openalex.org/W2120504118","https://openalex.org/W2121122889","https://openalex.org/W2121236750","https://openalex.org/W2122614523","https://openalex.org/W2125636433","https://openalex.org/W2126198460","https://openalex.org/W2126741025","https://openalex.org/W2129473723","https://openalex.org/W2129769231","https://openalex.org/W2130437495","https://openalex.org/W2131074131","https://openalex.org/W2131162901","https://openalex.org/W2131345241","https://openalex.org/W2131512287","https://openalex.org/W2132061275","https://openalex.org/W2132316084","https://openalex.org/W2132341951","https://openalex.org/W2133161896","https://openalex.org/W2133478087","https://openalex.org/W2134382129","https://openalex.org/W2134581641","https://openalex.org/W2139995627","https://openalex.org/W2141083815","https://openalex.org/W2142349419","https://openalex.org/W2142844453","https://openalex.org/W2143339100","https://openalex.org/W2143539785","https://openalex.org/W2143826692","https://openalex.org/W2144137438","https://openalex.org/W2144773959","https://openalex.org/W2150192750","https://openalex.org/W2150335921","https://openalex.org/W2150737519","https://openalex.org/W2153138268","https://openalex.org/W2153958514","https://openalex.org/W2154261378","https://openalex.org/W2156302599","https://openalex.org/W2157189708","https://openalex.org/W2157599344","https://openalex.org/W2159772052","https://openalex.org/W2161729702","https://openalex.org/W2162298934","https://openalex.org/W2163074782","https://openalex.org/W2167002475","https://openalex.org/W2167244112","https://openalex.org/W2167387669","https://openalex.org/W2168033803","https://openalex.org/W2168816122","https://openalex.org/W2169373177","https://openalex.org/W2169594496","https://openalex.org/W2170872073","https://openalex.org/W2298095968","https://openalex.org/W2335526184","https://openalex.org/W2407512034","https://openalex.org/W2412440863","https://openalex.org/W2415036755","https://openalex.org/W2475020042","https://openalex.org/W2481108442","https://openalex.org/W2491458126","https://openalex.org/W2534583847","https://openalex.org/W2779346725","https://openalex.org/W2802237266","https://openalex.org/W2885664031","https://openalex.org/W2890247752","https://openalex.org/W2895528841","https://openalex.org/W2950337489","https://openalex.org/W3023958910","https://openalex.org/W3100488885","https://openalex.org/W4238236906"],"related_works":["https://openalex.org/W2102928750","https://openalex.org/W2137793846","https://openalex.org/W4386484230","https://openalex.org/W2734835982","https://openalex.org/W2300906604","https://openalex.org/W4399226327","https://openalex.org/W2102832189","https://openalex.org/W2161347169","https://openalex.org/W2008872964","https://openalex.org/W2032647906"],"abstract_inverted_index":{"Abstract":[0],"The":[1,22],"past":[2],"decade":[3],"has":[4],"been":[5],"witnessed":[6],"to":[7,38,60],"exciting":[8],"developments":[9],"in":[10,32,75,80],"advanced":[11],"fluorescence":[12],"microscopy":[13],"techniques":[14,28,54,91],"that":[15,67],"rely":[16],"on":[17],"visualizing":[18],"single":[19],"emitting":[20],"fluorophores.":[21],"proliferation":[23],"of":[24,49,88],"single\u2010molecule":[25,90],"fluorescent":[26],"imaging":[27],"and":[29,44,92],"their":[30,93],"application":[31],"biological":[33,50,95],"research":[34,41],"have":[35],"the":[36,85],"potential":[37],"revolutionize":[39],"how":[40],"is":[42,65],"performed":[43],"greatly":[45],"increase":[46],"our":[47],"understanding":[48],"systems.":[51],"Presently,":[52],"these":[53],"are":[55,78],"still":[56],"relatively":[57],"niche":[58],"owing":[59],"technological":[61],"barriers,":[62],"but":[63],"it":[64],"foreseeable":[66],"they":[68],"will":[69],"become":[70],"an":[71],"increasingly":[72],"common":[73],"way":[74],"which":[76],"insights":[77],"sought":[79],"biology.":[81],"Here,":[82],"we":[83],"review":[84],"basic":[86],"principles":[87],"key":[89],"recent":[94],"applications.":[96]},"counts_by_year":[{"year":2021,"cited_by_count":2},{"year":2018,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}