{"id":"https://openalex.org/W1486768786","doi":"https://doi.org/10.1016/s0021-9258(18)34260-1","title":"Subunit heterogeneity in the lima bean lectin.","display_name":"Subunit heterogeneity in the lima bean lectin.","publication_year":1982,"publication_date":"1982-08-01","ids":{"openalex":"https://openalex.org/W1486768786","doi":"https://doi.org/10.1016/s0021-9258(18)34260-1","mag":"1486768786","pmid":"https://pubmed.ncbi.nlm.nih.gov/7096360"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)34260-1","doi":"https://doi.org/10.1016/s0021-9258(18)34260-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)34260-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)34260-1","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5031078822","display_name":"David D. Roberts","orcid":"https://orcid.org/0000-0002-2481-2981","observed_orcids":["https://orcid.org/0000-0002-2481-2981"]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I84218800","display_name":"University of California, Davis","ror":"https://ror.org/05rrcem69","country_code":"US","type":"education","lineage":["https://openalex.org/I84218800"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D D Roberts","raw_affiliation_strings":["From the \u00a7Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109","the IIDepartment of Biochemistry and Biophysics, University of California, Davis, California 95616"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the \u00a7Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"the IIDepartment of Biochemistry and Biophysics, University of California, Davis, California 95616","institution_ids":["https://openalex.org/I84218800"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111595661","display_name":"M.E. Etzler","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I84218800","display_name":"University of California, Davis","ror":"https://ror.org/05rrcem69","country_code":"US","type":"education","lineage":["https://openalex.org/I84218800"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M E Etzler","raw_affiliation_strings":["From the \u00a7Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109","the IIDepartment of Biochemistry and Biophysics, University of California, Davis, California 95616"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the \u00a7Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"the IIDepartment of Biochemistry and Biophysics, University of California, Davis, California 95616","institution_ids":["https://openalex.org/I84218800"]}]},{"author_position":"last","author":{"id":null,"display_name":"I J Goldstein","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]},{"id":"https://openalex.org/I84218800","display_name":"University of California, Davis","ror":"https://ror.org/05rrcem69","country_code":"US","type":"education","lineage":["https://openalex.org/I84218800"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"I J Goldstein","raw_affiliation_strings":["From the \u00a7Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109","the IIDepartment of Biochemistry and Biophysics, University of California, Davis, California 95616"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the \u00a7Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109","institution_ids":["https://openalex.org/I27837315"]},{"raw_affiliation_string":"the IIDepartment of Biochemistry and Biophysics, University of California, Davis, California 95616","institution_ids":["https://openalex.org/I84218800"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.8704,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.84632677,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"257","issue":"15","first_page":"9198","last_page":"9204"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lima-bean","display_name":"Phaseolus lunatus","score":0.9950000047683716},{"id":"https://openalex.org/keywords/subunit-heterogeneity","display_name":"subunit heterogeneity","score":0.9139999747276306},{"id":"https://openalex.org/keywords/dithiothreitol-reduction","display_name":"dithiothreitol reduction","score":0.8560000061988831},{"id":"https://openalex.org/keywords/isoelectric-focusing","display_name":"isoelectric focusing","score":0.8330000042915344},{"id":"https://openalex.org/keywords/seed-maturation","display_name":"seed maturation","score":0.8740000128746033},{"id":"https://openalex.org/keywords/glycoproteins","display_name":"glycoproteins","score":0.6710000038146973},{"id":"https://openalex.org/keywords/nh2-terminal-sequence","display_name":"NH2-terminal sequence","score":0.6840000152587891},{"id":"https://openalex.org/keywords/tryptic-peptide-mapping","display_name":"tryptic peptide mapping","score":0.7770000100135803}],"concepts":[{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.6155065894126892},{"id":"https://openalex.org/C2780758401","wikidata":"https://www.wikidata.org/wiki/Q408693","display_name":"Lectin","level":2,"score":0.5981269478797913},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42358145117759705},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37844833731651306},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3267216980457306},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08942440152168274}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D002241","descriptor_name":"Carbohydrates","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007525","descriptor_name":"Isoelectric Focusing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D037102","descriptor_name":"Lectins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D046911","descriptor_name":"Macromolecular Substances","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)34260-1","doi":"https://doi.org/10.1016/s0021-9258(18)34260-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)34260-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:7096360","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7096360","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)34260-1","doi":"https://doi.org/10.1016/s0021-9258(18)34260-1","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)34260-1","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4079792059","display_name":"Interdepartmental Training in Pharmacological Sciences","funder_award_id":"T32GM007767","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W137271191","https://openalex.org/W171093067","https://openalex.org/W1518714509","https://openalex.org/W1525111621","https://openalex.org/W1525197059","https://openalex.org/W1538872196","https://openalex.org/W1540450900","https://openalex.org/W1547610493","https://openalex.org/W1568537742","https://openalex.org/W1571793851","https://openalex.org/W1965044577","https://openalex.org/W1972533748","https://openalex.org/W1977227076","https://openalex.org/W1978597532","https://openalex.org/W1994391747","https://openalex.org/W1996302033","https://openalex.org/W2024340909","https://openalex.org/W2024558695","https://openalex.org/W2034211534","https://openalex.org/W2038993191","https://openalex.org/W2040793863","https://openalex.org/W2044711856","https://openalex.org/W2050335943","https://openalex.org/W2050808031","https://openalex.org/W2051616170","https://openalex.org/W2082522919","https://openalex.org/W2093566946","https://openalex.org/W2100837269","https://openalex.org/W2112984838","https://openalex.org/W2119586900","https://openalex.org/W2147882717","https://openalex.org/W2166047556"],"related_works":["https://openalex.org/W3155887911","https://openalex.org/W65560089","https://openalex.org/W396532861","https://openalex.org/W1930811621","https://openalex.org/W11784850","https://openalex.org/W2052574332","https://openalex.org/W2855385528","https://openalex.org/W1983046264","https://openalex.org/W1977315135","https://openalex.org/W1607997920"],"abstract_inverted_index":{"Three":[0,78],"forms":[1],"of":[2,39,58,65,74,82,103,129,166,170,173,178,188,222],"lectin":[3,61,76,225],"(components":[4],"I,":[5],"II,":[6],"and":[7,42,69,87,92,132,147,210,218],"III)":[8],"from":[9],"lima":[10,59,106,223],"beans":[11,107],"(Phaseolus":[12],"lunatus)":[13],"have":[14],"been":[15,48],"purified":[16],"on":[17],"an":[18],"affinity":[19],"support":[20],"containing":[21],"the":[22,63,75,112,141,153,163,167,216],"synthetic":[23],"type":[24],"A":[25,182],"blood":[26],"group":[27],"trisaccharide":[28],"alpha-D-GalNAc-(1":[29],"leads":[30,33,36],"to":[31,34,44,119,160,201],"3)-[alpha-L-Fuc-(1":[32],"2)]-beta-D-Gal-(1":[35],"to).":[37],"Conversion":[38],"components":[40],"I":[41],"II":[43],"component":[45],"III":[46],"has":[47],"achieved":[49],"by":[50],"reduction":[51],"in":[52,62,99,162,214],"10(-2)":[53],"M":[54,67],"dithiothreitol.":[55],"Isoelectric":[56],"focusing":[57],"bean":[60,224],"presence":[64],"8":[66],"urea":[68],"beta-mercaptoethanol":[70],"revealed":[71],"charge":[72,155,174],"heterogeneity":[73,156,209],"subunits.":[77,143,168],"major":[79],"subunit":[80,114,171,180,192,200,208,212],"classes":[81],"apparent":[83],"pI":[84,190,196],"7.05,":[85],"6.65,":[86],"6.45,":[88],"designated":[89],"alpha,":[90],"beta,":[91],"alpha',":[93],"respectively,":[94],"were":[95,138],"identified;":[96],"they":[97],"occur":[98],"a":[100,189,195],"relative":[101],"abundance":[102],"2:5:3.":[104],"Green":[105],"harvested":[108],"before":[109],"maturity":[110],"lacked":[111],"alpha'":[113],"(pI":[115],"6.45)":[116],"which":[117],"appears":[118],"accumulate":[120],"during":[121],"seed":[122],"maturation.":[123],"The":[124],"three":[125,142],"subunits":[126],"are":[127,226],"glycoproteins":[128],"identical":[130],"size":[131],"immunochemical":[133],"reactivity.":[134],"Identical":[135],"NH2-terminal":[136],"sequences":[137],"found":[139],"for":[140,207],"Amino":[144],"acid":[145],"analysis":[146],"tryptic":[148],"peptide":[149],"mapping":[150],"indicated":[151],"that":[152],"observed":[154],"is":[157,184],"probably":[158],"due":[159],"differences":[161],"primary":[164],"structure":[165],"Studies":[169],"composition":[172],"isolectins":[175],"provided":[176],"evidence":[177],"nonrandom":[179],"assembly.":[181],"model":[183],"proposed":[185],"involving":[186],"pairing":[187],"6.65":[191],"with":[193],"either":[194],"7.06":[197],"or":[198],"6.45":[199],"form":[202],"dimeric":[203],"units.":[204],"Possible":[205],"roles":[206],"ordered":[211],"assembly":[213],"determining":[215],"metal":[217],"sugar":[219],"binding":[220],"properties":[221],"discussed.":[227]},"counts_by_year":[{"year":2017,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}