{"id":"https://openalex.org/W1484881822","doi":null,"title":"METHODS DEVELOPMENT AND APPLICATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS AND MASS SPECTROMETRY IN PROTEOMICS","display_name":"METHODS DEVELOPMENT AND APPLICATION OF TWO-DIMENSIONAL GEL ELECTROPHORESIS AND MASS SPECTROMETRY IN PROTEOMICS","publication_year":2006,"publication_date":"2006-01-01","ids":{"openalex":"https://openalex.org/W1484881822","mag":"1484881822"},"language":"en","primary_location":{"id":"pmh:oai:uknowledge.uky.edu:gradschool_diss-1295","doi":null,"is_oa":true,"landing_page_url":"https://uknowledge.uky.edu/gradschool_diss/292","pdf_url":"https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1295&context=gradschool_diss","source":{"id":"https://openalex.org/S4306402623","display_name":"UKnowledge (University of Kentucky)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I143302722","host_organization_name":"University of Kentucky","host_organization_lineage":["https://openalex.org/I143302722"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"University of Kentucky Doctoral Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1295&context=gradschool_diss","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5075708272","display_name":"Meena U. Rajagopal","orcid":"https://orcid.org/0000-0003-2778-1831","observed_orcids":["https://orcid.org/0000-0003-2778-1831"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Meena Rajagopal","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-2778-1831","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5075708272"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.06579745,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-staining","display_name":"protein staining","score":0.7049999833106995},{"id":"https://openalex.org/keywords/two-dimensional-electrophoresis","display_name":"two-dimensional gel electrophoresis","score":0.9100000262260437},{"id":"https://openalex.org/keywords/mass-spectrometry","display_name":"mass spectrometry","score":0.7289999723434448},{"id":"https://openalex.org/keywords/proteomics","display_name":"proteomics","score":0.7749999761581421},{"id":"https://openalex.org/keywords/malathion","display_name":"malathion","score":0.968999981880188},{"id":"https://openalex.org/keywords/neuroblastoma","display_name":"neuroblastoma","score":0.7480000257492065},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.7609999775886536},{"id":"https://openalex.org/keywords/calmodulin","display_name":"calmodulin","score":0.8920000195503235},{"id":"https://openalex.org/keywords/pesticide-effects","display_name":"pesticide effects","score":0.6389999985694885},{"id":"https://openalex.org/keywords/cerebrospinal-fluid","display_name":"cerebrospinal fluid","score":0.890999972820282},{"id":"https://openalex.org/keywords/alzheimer-s-disease","display_name":"Alzheimer's disease","score":0.8939999938011169},{"id":"https://openalex.org/keywords/biomarkers","display_name":"biomarkers","score":0.7440000176429749},{"id":"https://openalex.org/keywords/prostaglandin-d-synthase","display_name":"prostaglandin D synthase","score":0.9269999861717224},{"id":"https://openalex.org/keywords/protein-isoforms","display_name":"protein isoforms","score":0.9639999866485596}],"concepts":[{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.6903077363967896},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.5925776958465576},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.41225332021713257},{"id":"https://openalex.org/C47793336","wikidata":"https://www.wikidata.org/wiki/Q4688061","display_name":"Two-dimensional gel electrophoresis","level":4,"score":0.4111628234386444},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.39244937896728516},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.05353870987892151},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:uknowledge.uky.edu:gradschool_diss-1295","doi":null,"is_oa":true,"landing_page_url":"https://uknowledge.uky.edu/gradschool_diss/292","pdf_url":"https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1295&context=gradschool_diss","source":{"id":"https://openalex.org/S4306402623","display_name":"UKnowledge (University of Kentucky)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I143302722","host_organization_name":"University of Kentucky","host_organization_lineage":["https://openalex.org/I143302722"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"University of Kentucky Doctoral Dissertations","raw_type":"text"},{"id":"mag:1484881822","doi":null,"is_oa":false,"landing_page_url":"https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1295&context=gradschool_diss","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:uknowledge.uky.edu:gradschool_diss-1295","doi":null,"is_oa":true,"landing_page_url":"https://uknowledge.uky.edu/gradschool_diss/292","pdf_url":"https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1295&context=gradschool_diss","source":{"id":"https://openalex.org/S4306402623","display_name":"UKnowledge (University of Kentucky)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I143302722","host_organization_name":"University of Kentucky","host_organization_lineage":["https://openalex.org/I143302722"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"University of Kentucky Doctoral Dissertations","raw_type":"text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1484881822.pdf","grobid_xml":"https://content.openalex.org/works/W1484881822.grobid-xml"},"referenced_works_count":243,"referenced_works":["https://openalex.org/W7611090","https://openalex.org/W70578846","https://openalex.org/W130525919","https://openalex.org/W139706748","https://openalex.org/W173643440","https://openalex.org/W567057721","https://openalex.org/W613793198","https://openalex.org/W944969987","https://openalex.org/W1033914640","https://openalex.org/W1481540262","https://openalex.org/W1483087608","https://openalex.org/W1503901149","https://openalex.org/W1511203783","https://openalex.org/W1511645251","https://openalex.org/W1511827873","https://openalex.org/W1535097870","https://openalex.org/W1537274817","https://openalex.org/W1539323393","https://openalex.org/W1544975911","https://openalex.org/W1550351158","https://openalex.org/W1556008598","https://openalex.org/W1564601025","https://openalex.org/W1568775518","https://openalex.org/W1569513589","https://openalex.org/W1580575515","https://openalex.org/W1600112653","https://openalex.org/W1603717990","https://openalex.org/W1606423922","https://openalex.org/W1640652599","https://openalex.org/W1671787304","https://openalex.org/W1759706356","https://openalex.org/W1770711785","https://openalex.org/W1868427136","https://openalex.org/W1909841186","https://openalex.org/W1939645046","https://openalex.org/W1963770237","https://openalex.org/W1963812525","https://openalex.org/W1964220924","https://openalex.org/W1964313477","https://openalex.org/W1964992965","https://openalex.org/W1965261736","https://openalex.org/W1965423473","https://openalex.org/W1966603503","https://openalex.org/W1966665626","https://openalex.org/W1967343287","https://openalex.org/W1969374006","https://openalex.org/W1970259570","https://openalex.org/W1970662326","https://openalex.org/W1971430357","https://openalex.org/W1971840315","https://openalex.org/W1972958434","https://openalex.org/W1974732006","https://openalex.org/W1974830441","https://openalex.org/W1976990301","https://openalex.org/W1977117878","https://openalex.org/W1978823829","https://openalex.org/W1979972336","https://openalex.org/W1981593008","https://openalex.org/W1982341565","https://openalex.org/W1983829738","https://openalex.org/W1984496481","https://openalex.org/W1985085549","https://openalex.org/W1985516583","https://openalex.org/W1988556197","https://openalex.org/W1989333435","https://openalex.org/W1990210528","https://openalex.org/W1990583552","https://openalex.org/W1991178147","https://openalex.org/W1991435932","https://openalex.org/W1992412469","https://openalex.org/W1992730111","https://openalex.org/W1993625807","https://openalex.org/W1993631374","https://openalex.org/W1993939152","https://openalex.org/W1995024339","https://openalex.org/W1996111288","https://openalex.org/W1996545485","https://openalex.org/W1996952423","https://openalex.org/W1997270203","https://openalex.org/W1997388462","https://openalex.org/W1997458078","https://openalex.org/W1997890851","https://openalex.org/W1997958695","https://openalex.org/W1998393335","https://openalex.org/W1999183933","https://openalex.org/W2001179326","https://openalex.org/W2002358001","https://openalex.org/W2005640894","https://openalex.org/W2007159852","https://openalex.org/W2007229234","https://openalex.org/W2008148453","https://openalex.org/W2008479138","https://openalex.org/W2008746494","https://openalex.org/W2010233116","https://openalex.org/W2010460292","https://openalex.org/W2010762857","https://openalex.org/W2011606718","https://openalex.org/W2011748398","https://openalex.org/W2011770757","https://openalex.org/W2013130041","https://openalex.org/W2017661443","https://openalex.org/W2017888024","https://openalex.org/W2018571987","https://openalex.org/W2020610209","https://openalex.org/W2020691810","https://openalex.org/W2021556583","https://openalex.org/W2021812827","https://openalex.org/W2022510326","https://openalex.org/W2029541656","https://openalex.org/W2030559877","https://openalex.org/W2031898930","https://openalex.org/W2032194486","https://openalex.org/W2033604459","https://openalex.org/W2034899525","https://openalex.org/W2036178608","https://openalex.org/W2036288989","https://openalex.org/W2039602145","https://openalex.org/W2040706334","https://openalex.org/W2041337103","https://openalex.org/W2041797254","https://openalex.org/W2044031095","https://openalex.org/W2045440553","https://openalex.org/W2045623268","https://openalex.org/W2045641777","https://openalex.org/W2046726975","https://openalex.org/W2046750894","https://openalex.org/W2047546483","https://openalex.org/W2048135913","https://openalex.org/W2049876394","https://openalex.org/W2050262822","https://openalex.org/W2050466710","https://openalex.org/W2050594129","https://openalex.org/W2051616170","https://openalex.org/W2051952557","https://openalex.org/W2052347574","https://openalex.org/W2054673893","https://openalex.org/W2056557745","https://openalex.org/W2056706857","https://openalex.org/W2059457068","https://openalex.org/W2060644195","https://openalex.org/W2061319849","https://openalex.org/W2062775685","https://openalex.org/W2063450941","https://openalex.org/W2063452365","https://openalex.org/W2065496524","https://openalex.org/W2067511286","https://openalex.org/W2067569818","https://openalex.org/W2067811915","https://openalex.org/W2067814054","https://openalex.org/W2068241704","https://openalex.org/W2068354294","https://openalex.org/W2068751031","https://openalex.org/W2068887628","https://openalex.org/W2070649457","https://openalex.org/W2071953900","https://openalex.org/W2073171220","https://openalex.org/W2073292063","https://openalex.org/W2073384304","https://openalex.org/W2078594804","https://openalex.org/W2080557519","https://openalex.org/W2080880751","https://openalex.org/W2082635345","https://openalex.org/W2082760019","https://openalex.org/W2083362678","https://openalex.org/W2083828722","https://openalex.org/W2083984027","https://openalex.org/W2084772913","https://openalex.org/W2087936588","https://openalex.org/W2087960591","https://openalex.org/W2089792993","https://openalex.org/W2090881930","https://openalex.org/W2092166718","https://openalex.org/W2092514612","https://openalex.org/W2094782116","https://openalex.org/W2096664280","https://openalex.org/W2096966529","https://openalex.org/W2098693385","https://openalex.org/W2100377941","https://openalex.org/W2100634607","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2102075074","https://openalex.org/W2104382960","https://openalex.org/W2105421015","https://openalex.org/W2106497258","https://openalex.org/W2110174460","https://openalex.org/W2110311890","https://openalex.org/W2111972716","https://openalex.org/W2113007696","https://openalex.org/W2113823618","https://openalex.org/W2117462751","https://openalex.org/W2118747886","https://openalex.org/W2121918549","https://openalex.org/W2127033103","https://openalex.org/W2127230663","https://openalex.org/W2129532952","https://openalex.org/W2130381045","https://openalex.org/W2130669719","https://openalex.org/W2130764195","https://openalex.org/W2133740364","https://openalex.org/W2134010906","https://openalex.org/W2137417707","https://openalex.org/W2137662702","https://openalex.org/W2138498116","https://openalex.org/W2138760035","https://openalex.org/W2139052757","https://openalex.org/W2140735579","https://openalex.org/W2146421447","https://openalex.org/W2146729934","https://openalex.org/W2147604930","https://openalex.org/W2150123812","https://openalex.org/W2153943744","https://openalex.org/W2154904733","https://openalex.org/W2155747798","https://openalex.org/W2156220037","https://openalex.org/W2157973516","https://openalex.org/W2160880695","https://openalex.org/W2161814309","https://openalex.org/W2163704344","https://openalex.org/W2167553705","https://openalex.org/W2167699112","https://openalex.org/W2168325755","https://openalex.org/W2169048699","https://openalex.org/W2169393803","https://openalex.org/W2169937961","https://openalex.org/W2171991500","https://openalex.org/W2172250804","https://openalex.org/W2178113031","https://openalex.org/W2199096412","https://openalex.org/W2263362788","https://openalex.org/W2320149262","https://openalex.org/W2336135978","https://openalex.org/W2396016074","https://openalex.org/W2413237464","https://openalex.org/W2430392320","https://openalex.org/W2439044246","https://openalex.org/W2443634062","https://openalex.org/W2472347257","https://openalex.org/W2474979626","https://openalex.org/W2609271038","https://openalex.org/W2912078763","https://openalex.org/W4237728860","https://openalex.org/W4366064133"],"related_works":["https://openalex.org/W2054635859","https://openalex.org/W2045623268","https://openalex.org/W7100911488","https://openalex.org/W2082760019","https://openalex.org/W2049331615","https://openalex.org/W1992412469","https://openalex.org/W1971083640","https://openalex.org/W1998357169","https://openalex.org/W2070641466","https://openalex.org/W2014900018"],"abstract_inverted_index":{"The":[0,14,62,84,104,259],"development":[1],"of":[2,28,60,64,66,98,111,128,133,140,164,190,205,210,226,247,250,280,291,309],"a":[3,72,101,198,202,270],"highly":[4,177],"sensitive":[5],"ruthenium-based":[6],"fluorescent":[7,80],"staining":[8,68,105],"solution":[9,69,106],"isdescribed":[10],"in":[11,193,207,218,228,235,252,284],"this":[12,99,219,295],"dissertation.":[13],"in-house":[15],"synthesized":[16],"ruthenium":[17,40],"complex":[18,41],"(RuMS)containing":[19],"both":[20],"sulfonated":[21,43],"and":[22,39,52,91,120,168,170,224,294],"non-sulfonated":[23],"ligand":[24],"has":[25,48],"detection":[26,86],"limit":[27],"1":[29],"ng":[30],"ofprotein":[31],"that":[32,138,143,232],"is":[33,197,201,244],"better":[34],"than":[35],"colloidal":[36],"coomassie,":[37],"silver":[38],"containingall":[42],"ligands":[44],"(RuBPS).":[45],"RuMS":[46],"stain":[47,100],"100-fold":[49],"dynamic":[50,89],"range":[51,90],"does":[53],"notinterfere":[54],"with":[55,93,160],"subsequent":[56],"mass":[57,94,121],"spectral":[58],"identification":[59,189,209,225],"proteins.":[61],"capability":[63],"inhousesynthesis":[65],"the":[67,126,131,147,208,262,277,292,304,310],"makes":[70,174],"it":[71],"viable":[73],"cost-effective":[74],"alternative":[75],"to":[76,179,303],"theexpensive":[77],"commercially":[78],"available":[79],"stain,":[81],"Sypro":[82],"Ruby.":[83],"low":[85],"limit,broad":[87],"linear":[88],"compatibility":[92],"spectrometry,":[95],"make":[96],"thedevelopment":[97],"worthwhile":[102],"pursuit.":[103],"was":[107,123],"utilized":[108],"insubsequent":[109],"applications":[110],"two-dimensional":[112],"gel":[113],"electrophoresis":[114],"(2-DE)":[115],"technology.Proteomics":[116],"methodology":[117],"utilizing":[118],"2-DE":[119],"spectrometry":[122],"applied":[124],"toinvestigate":[125],"effect":[127],"malathion":[129],"on":[130,300],"proteome":[132],"human":[134],"neuroblastoma":[135,195],"cells.Results":[136],"indicated":[137],"out":[139],"122":[141],"proteins":[142,150,155,192,251],"were":[144,151,156],"identified":[145],"from":[146],"neuroblastomaproteome,":[148],"sixteen":[149],"down-regulated":[152],"while":[153],"five":[154],"significantlyup-regulated":[157],"after":[158],"treatment":[159],"malathion.":[161],"Significant":[162],"down-regulation":[163],"calciummodulators":[165],"like":[166],"calmodulin":[167],"calgizarrin":[169],"other":[171],"key":[172,242],"chaperones":[173],"themalathion-treated":[175],"cells":[176,196],"prone":[178],"oxidative":[180],"stress.":[181],"With":[182],"increased":[183],"awareness":[184],"inpesticide":[185],"related":[186],"adverse":[187],"effects,":[188],"altered":[191],"malathion-treatedhuman":[194],"critical":[199],"finding.Proteomics":[200],"major":[203],"area":[204],"research":[206],"biomarkers":[211],"for":[212,222,273],"diseases.":[213],"Anovel":[214],"immunoprecipitation":[215],"method":[216],"developed":[217],"work":[220],"allowed":[221],"successfulisolation":[223],"albumin-interactome":[227,254],"cerebrospinal":[229],"fluid":[230],"(CSF)":[231],"isusually":[233],"under-represented":[234],"standard":[236],"CSF":[237,253],"analysis":[238],"using":[239],"2-DE.":[240],"A":[241],"finding":[243],"thedifferential":[245],"expression":[246,279],"various":[248],"isoforms":[249,283],"fromAlzheimer's":[255],"disease":[256,293],"(AD)":[257],"subjects.":[258],"data":[260],"implicate":[261],"acidic":[263],"isoform":[264],"ofprostaglandin":[265],"D2":[266],"synthase":[267],"(PGDS2)":[268],"as":[269],"potential":[271],"biomarker":[272],"AD.":[274],"An":[275],"understandingof":[276],"differential":[278],"these":[281],"protein":[282],"AD":[285],"will":[286],"provide":[287],"insight":[288],"into":[289],"theetiology":[290],"can":[296],"have":[297],"far-reaching":[298],"implication":[299],"drug":[301],"developmentleading":[302],"cure":[305],"or":[306],"even":[307],"preventation":[308],"disease.":[311]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
