{"id":"https://openalex.org/W1483973713","doi":null,"title":"Establishment of a positive-readout reporter system for siRNAs.","display_name":"Establishment of a positive-readout reporter system for siRNAs.","publication_year":2009,"publication_date":"2009-06-12","ids":{"openalex":"https://openalex.org/W1483973713","mag":"1483973713","pmid":"https://pubmed.ncbi.nlm.nih.gov/19771230","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2737236"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2737236","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2737236","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J RNAi Gene Silencing","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2737236","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101732617","display_name":"Weili Liu","orcid":"https://orcid.org/0000-0003-0107-7408","observed_orcids":["https://orcid.org/0000-0003-0107-7408"]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Wei-Li Liu","raw_affiliation_strings":["Department of Clinical Pharmacology, Old Road Campus Research Building, University of Oxford, Old Road Campus off Roosevelt Drive, Headington, Oxford OX3 7DQ, UK"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Clinical Pharmacology, Old Road Campus Research Building, University of Oxford, Old Road Campus off Roosevelt Drive, Headington, Oxford OX3 7DQ, UK","institution_ids":["https://openalex.org/I40120149"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040508353","display_name":"Douglas Owen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Douglas P Owen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110967220","display_name":"Kerry Fisher","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kerry D Fisher","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032131181","display_name":"Leonard William Seymour","orcid":"https://orcid.org/0000-0003-3825-0841","observed_orcids":["https://orcid.org/0000-0003-3825-0841"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Leonard W Seymour","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5080682537","display_name":"Mark Stevenson","orcid":"https://orcid.org/0000-0001-8616-0205","observed_orcids":["https://orcid.org/0000-0001-8616-0205"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mark Stevenson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.07168873,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"5","issue":"1","first_page":"331","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sirna-delivery","display_name":"siRNA delivery","score":0.777999997138977},{"id":"https://openalex.org/keywords/luciferase-reporter","display_name":"luciferase reporter","score":0.8479999899864197},{"id":"https://openalex.org/keywords/lac-repressor","display_name":"lac repressor","score":0.902999997138977},{"id":"https://openalex.org/keywords/lac-operon","display_name":"lac operon","score":0.9079999923706055},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.7689999938011169},{"id":"https://openalex.org/keywords/transgenic-mouse-model","display_name":"transgenic mouse model","score":0.6380000114440918},{"id":"https://openalex.org/keywords/rnai-therapeutics","display_name":"RNAi therapeutics","score":0.5490000247955322}],"concepts":[{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.9527156949043274},{"id":"https://openalex.org/C158448853","wikidata":"https://www.wikidata.org/wiki/Q425218","display_name":"Repressor","level":4,"score":0.7602408528327942},{"id":"https://openalex.org/C113629113","wikidata":"https://www.wikidata.org/wiki/Q4345509","display_name":"Lac repressor","level":5,"score":0.6723833680152893},{"id":"https://openalex.org/C2225953","wikidata":"https://www.wikidata.org/wiki/Q1661899","display_name":"Inducer","level":3,"score":0.5813471674919128},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.5591245293617249},{"id":"https://openalex.org/C22615655","wikidata":"https://www.wikidata.org/wiki/Q203221","display_name":"Small interfering RNA","level":4,"score":0.5575262904167175},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5378673672676086},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.5363259315490723},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.5112056732177734},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.4858705401420593},{"id":"https://openalex.org/C203075996","wikidata":"https://www.wikidata.org/wiki/Q139677","display_name":"Operon","level":4,"score":0.47024407982826233},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.4633622169494629},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.43951427936553955},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4212827980518341},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4085237681865692},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3918444812297821},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.31171220541000366},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2744212746620178},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19135040044784546},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18959230184555054},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.1759902834892273}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:2737236","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2737236","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J RNAi Gene Silencing","raw_type":"Text"},{"id":"pmid:19771230","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19771230","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research","raw_type":"Journal Article"},{"id":"pmh:oai:ora.ox.ac.uk:uuid:c3071349-9c95-4606-9653-8835c4c37ab6","doi":null,"is_oa":true,"landing_page_url":"https://ora.ox.ac.uk/objects/uuid:c3071349-9c95-4606-9653-8835c4c37ab6","pdf_url":"https://ora.ox.ac.uk/objects/uuid:c3071349-9c95-4606-9653-8835c4c37ab6","source":{"id":"https://openalex.org/S4306402636","display_name":"Oxford University Research Archive (ORA) (University of Oxford)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I40120149","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/I40120149"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Symplectic Elements at Oxford","raw_type":"Journal article"},{"id":"mag:1483973713","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19771230","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2737236","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2737236","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J RNAi Gene Silencing","raw_type":"Text"},"sustainable_development_goals":[{"score":0.5683000087738037,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1483973713.pdf","grobid_xml":"https://content.openalex.org/works/W1483973713.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W1505088608","https://openalex.org/W1611419331","https://openalex.org/W1647075334","https://openalex.org/W1989407131","https://openalex.org/W1992657519","https://openalex.org/W2001583023","https://openalex.org/W2002275618","https://openalex.org/W2007888673","https://openalex.org/W2016232662","https://openalex.org/W2018889720","https://openalex.org/W2019631541","https://openalex.org/W2032619610","https://openalex.org/W2033396984","https://openalex.org/W2040672743","https://openalex.org/W2047165821","https://openalex.org/W2059978839","https://openalex.org/W2080758920","https://openalex.org/W2091220084","https://openalex.org/W2095151181","https://openalex.org/W2112962172","https://openalex.org/W2116424844","https://openalex.org/W2122052737","https://openalex.org/W2129596289","https://openalex.org/W2138436760","https://openalex.org/W2139930568","https://openalex.org/W2144449905","https://openalex.org/W2146871305","https://openalex.org/W2158284529","https://openalex.org/W2166802510","https://openalex.org/W7096831481"],"related_works":["https://openalex.org/W2094259092","https://openalex.org/W2115019462","https://openalex.org/W2753918409","https://openalex.org/W2034264394","https://openalex.org/W54889092","https://openalex.org/W597855358","https://openalex.org/W4307770849","https://openalex.org/W2096438955","https://openalex.org/W2071142904","https://openalex.org/W2012547585"],"abstract_inverted_index":{"The":[0,164],"use":[1],"of":[2,12,55,62,77,101,115,120,143,162,174,190],"small":[3],"interfering":[4],"RNA":[5],"molecules":[6],"for":[7,28],"therapeutic":[8],"applications":[9],"requires":[10],"development":[11],"improved":[13],"delivery":[14,54,177],"systems,":[15],"a":[16,23,46,63,69,152,171,186],"process":[17],"that":[18],"would":[19,34],"be":[20,182],"facilitated":[21],"by":[22,52],"non-invasive":[24],"positive-readout":[25,47,166],"mouse":[26,188],"model":[27],"studying":[29],"siRNA":[30,56,122],"pharmacodynamics.":[31],"Positive":[32],"readout":[33],"yield":[35],"better":[36],"signal/noise":[37],"ratios":[38],"than":[39,92],"existing":[40],"negative-readout":[41],"systems.":[42],"We":[43],"have":[44],"engineered":[45],"luciferase":[48,73,90,107,135,165],"reporter":[49,167],"system,":[50],"activated":[51],"successful":[53,175],"targeting":[57,123],"the":[58,75,116,141,158],"lac":[59,66,83,117,124],"repressor.":[60,144],"Co-transfection":[61],"plasmid":[64,70,94],"expressing":[65,71],"repressor":[67,125],"and":[68,112,180],"firefly":[72],"under":[74],"control":[76],"an":[78],"RSV":[79],"promoter,":[80],"containing":[81],"two":[82],"operator":[84],"sites,":[85],"resulted":[86],"in":[87,140,178,195],"5.7-fold":[88],"lower":[89],"activity":[91,193],"luciferase-encoding":[93],"alone.":[95],"Inhibition":[96],"was":[97,130,147],"reversed":[98],"following":[99],"addition":[100],"synthetic":[102],"inducer,":[103],"IPTG,":[104],"which":[105],"elevated":[106],"expression":[108,136,146],"to":[109,126,132,137,157,184],"normal":[110],"levels":[111,138],"confirmed":[113],"functionality":[114],"operon.":[118],"Delivery":[119],"1nM":[121],"repressor/reporter":[127],"co-transfected":[128],"cells":[129],"sufficient":[131],"fully":[133],"restore":[134],"observed":[139,148],"absence":[142],"Maximum":[145],"after":[149],"48hr,":[150],"with":[151],"rapid":[153],"decrease":[154],"thereafter":[155],"due":[156],"short":[159],"half":[160],"life":[161],"luciferase.":[163],"system":[168],"is":[169],"therefore":[170],"dynamic":[172],"indicator":[173],"RNAi":[176,192],"vitro":[179],"could":[181],"adapted":[183],"generate":[185],"transgenic":[187],"capable":[189],"reporting":[191],"non-invasively":[194],"vivo.":[196]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
