{"id":"https://openalex.org/W148255003","doi":null,"title":"Towards the derivation of bovine embryonic stem cells","display_name":"Towards the derivation of bovine embryonic stem cells","publication_year":2008,"publication_date":"2008-01-28","ids":{"openalex":"https://openalex.org/W148255003","mag":"148255003"},"language":"en","primary_location":{"id":"pmh:oai:drum.lib.umd.edu:1903/7854","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1903/7854","pdf_url":"https://drum.lib.umd.edu/bitstreams/06f4f8b9-edff-4c11-b483-c7fbc3499a51/download","source":{"id":"https://openalex.org/S4306401518","display_name":"University Libraries (University of Maryland)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I66946132","host_organization_name":"University of Maryland, College Park","host_organization_lineage":["https://openalex.org/I66946132"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://drum.lib.umd.edu/bitstreams/06f4f8b9-edff-4c11-b483-c7fbc3499a51/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014359310","display_name":"Disha Pant","orcid":"https://orcid.org/0000-0003-0763-0470","observed_orcids":["https://orcid.org/0000-0003-0763-0470"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Disha Pant","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-0763-0470","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5014359310"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.009800000116229057,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bovine-embryonic-stem-cells","display_name":"bovine embryonic stem cells","score":0.996999979019165},{"id":"https://openalex.org/keywords/inner-cell-mass","display_name":"inner cell mass","score":0.8790000081062317},{"id":"https://openalex.org/keywords/blastocyst","display_name":"blastocyst","score":0.8169999718666077},{"id":"https://openalex.org/keywords/stem-cell-markers","display_name":"stem cell markers","score":0.9089999794960022},{"id":"https://openalex.org/keywords/nanog","display_name":"Nanog","score":0.9990000128746033},{"id":"https://openalex.org/keywords/oct4","display_name":"Oct4","score":0.9710000157356262},{"id":"https://openalex.org/keywords/sox2","display_name":"SOX2","score":1.0},{"id":"https://openalex.org/keywords/bmp4-signaling","display_name":"BMP4 signaling","score":0.7870000004768372},{"id":"https://openalex.org/keywords/noggin","display_name":"noggin","score":0.9340000152587891},{"id":"https://openalex.org/keywords/cytokine-supplementation","display_name":"cytokine supplementation","score":0.6940000057220459},{"id":"https://openalex.org/keywords/somatic-cell-nuclear-transfer","display_name":"somatic cell nuclear transfer","score":0.8920000195503235},{"id":"https://openalex.org/keywords/hygromycin-selection","display_name":"hygromycin selection","score":0.8040000200271606},{"id":"https://openalex.org/keywords/embryo-culture-conditions","display_name":"embryo culture conditions","score":0.3330000042915344},{"id":"https://openalex.org/keywords/cattle","display_name":"cattle","score":0.3790000081062317}],"concepts":[{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.7019196152687073},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.5696006417274475},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.549708366394043},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.44252923130989075},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.38006460666656494},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3438171148300171},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2360457479953766},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09694388508796692}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:drum.lib.umd.edu:1903/7854","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1903/7854","pdf_url":"https://drum.lib.umd.edu/bitstreams/06f4f8b9-edff-4c11-b483-c7fbc3499a51/download","source":{"id":"https://openalex.org/S4306401518","display_name":"University Libraries (University of Maryland)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I66946132","host_organization_name":"University of Maryland, College Park","host_organization_lineage":["https://openalex.org/I66946132"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},{"id":"mag:148255003","doi":null,"is_oa":false,"landing_page_url":"https://drum.lib.umd.edu/handle/1903/7854","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:drum.lib.umd.edu:1903/7854","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1903/7854","pdf_url":"https://drum.lib.umd.edu/bitstreams/06f4f8b9-edff-4c11-b483-c7fbc3499a51/download","source":{"id":"https://openalex.org/S4306401518","display_name":"University Libraries (University of Maryland)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I66946132","host_organization_name":"University of Maryland, College Park","host_organization_lineage":["https://openalex.org/I66946132"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W148255003.pdf","grobid_xml":"https://content.openalex.org/works/W148255003.grobid-xml"},"referenced_works_count":190,"referenced_works":["https://openalex.org/W20465460","https://openalex.org/W122029960","https://openalex.org/W1547855212","https://openalex.org/W1582404500","https://openalex.org/W1597871117","https://openalex.org/W1601995899","https://openalex.org/W1608762409","https://openalex.org/W1608843970","https://openalex.org/W1619397995","https://openalex.org/W1832914678","https://openalex.org/W1877667148","https://openalex.org/W1894129931","https://openalex.org/W1894858966","https://openalex.org/W1957763880","https://openalex.org/W1963559963","https://openalex.org/W1966621898","https://openalex.org/W1966915633","https://openalex.org/W1967380741","https://openalex.org/W1968814677","https://openalex.org/W1970200774","https://openalex.org/W1974365957","https://openalex.org/W1974986171","https://openalex.org/W1975163937","https://openalex.org/W1976628761","https://openalex.org/W1977135603","https://openalex.org/W1980417131","https://openalex.org/W1981130819","https://openalex.org/W1981138517","https://openalex.org/W1981531825","https://openalex.org/W1982960471","https://openalex.org/W1985208420","https://openalex.org/W1990211949","https://openalex.org/W1991502013","https://openalex.org/W1992160731","https://openalex.org/W1992525863","https://openalex.org/W1992838550","https://openalex.org/W1992949513","https://openalex.org/W1993534485","https://openalex.org/W1993834457","https://openalex.org/W1994435996","https://openalex.org/W1994758141","https://openalex.org/W1994831934","https://openalex.org/W1995559307","https://openalex.org/W1997054356","https://openalex.org/W1997506291","https://openalex.org/W1999609687","https://openalex.org/W1999920730","https://openalex.org/W2000246164","https://openalex.org/W2001073369","https://openalex.org/W2003701368","https://openalex.org/W2003932465","https://openalex.org/W2005432188","https://openalex.org/W2006499356","https://openalex.org/W2010022393","https://openalex.org/W2010353459","https://openalex.org/W2010390759","https://openalex.org/W2010608530","https://openalex.org/W2013588323","https://openalex.org/W2014073178","https://openalex.org/W2015036988","https://openalex.org/W2016937312","https://openalex.org/W2017968214","https://openalex.org/W2020712616","https://openalex.org/W2021317109","https://openalex.org/W2021952728","https://openalex.org/W2022049388","https://openalex.org/W2023745283","https://openalex.org/W2031280968","https://openalex.org/W2031311971","https://openalex.org/W2031569048","https://openalex.org/W2031862844","https://openalex.org/W2033986471","https://openalex.org/W2035247129","https://openalex.org/W2038752568","https://openalex.org/W2039850708","https://openalex.org/W2040188417","https://openalex.org/W2040479298","https://openalex.org/W2041076733","https://openalex.org/W2045899482","https://openalex.org/W2046193560","https://openalex.org/W2046396702","https://openalex.org/W2050351042","https://openalex.org/W2050920387","https://openalex.org/W2051921610","https://openalex.org/W2052569390","https://openalex.org/W2053378592","https://openalex.org/W2053905634","https://openalex.org/W2053911973","https://openalex.org/W2053997148","https://openalex.org/W2055852018","https://openalex.org/W2056089399","https://openalex.org/W2056950641","https://openalex.org/W2056983875","https://openalex.org/W2057159461","https://openalex.org/W2060078355","https://openalex.org/W2060132470","https://openalex.org/W2060299616","https://openalex.org/W2060341627","https://openalex.org/W2060926602","https://openalex.org/W2061004792","https://openalex.org/W2061567151","https://openalex.org/W2063395944","https://openalex.org/W2066364577","https://openalex.org/W2067988300","https://openalex.org/W2071241326","https://openalex.org/W2071505994","https://openalex.org/W2073521954","https://openalex.org/W2074434853","https://openalex.org/W2075679174","https://openalex.org/W2077254097","https://openalex.org/W2077517942","https://openalex.org/W2077636451","https://openalex.org/W2077838820","https://openalex.org/W2082019843","https://openalex.org/W2082091416","https://openalex.org/W2082773762","https://openalex.org/W2083149287","https://openalex.org/W2083763893","https://openalex.org/W2086293569","https://openalex.org/W2086840459","https://openalex.org/W2086943348","https://openalex.org/W2087560101","https://openalex.org/W2087789903","https://openalex.org/W2088514277","https://openalex.org/W2089426706","https://openalex.org/W2092806325","https://openalex.org/W2093308617","https://openalex.org/W2093540365","https://openalex.org/W2094557730","https://openalex.org/W2095701213","https://openalex.org/W2095762981","https://openalex.org/W2096017463","https://openalex.org/W2100338676","https://openalex.org/W2102020781","https://openalex.org/W2106124311","https://openalex.org/W2106278040","https://openalex.org/W2108451330","https://openalex.org/W2110015307","https://openalex.org/W2110893450","https://openalex.org/W2111191613","https://openalex.org/W2112104218","https://openalex.org/W2113693767","https://openalex.org/W2116228913","https://openalex.org/W2116978641","https://openalex.org/W2118290853","https://openalex.org/W2122441400","https://openalex.org/W2122496049","https://openalex.org/W2122864540","https://openalex.org/W2122892477","https://openalex.org/W2123986423","https://openalex.org/W2125987139","https://openalex.org/W2126851387","https://openalex.org/W2127799088","https://openalex.org/W2128323764","https://openalex.org/W2129957319","https://openalex.org/W2131282078","https://openalex.org/W2132545788","https://openalex.org/W2132555347","https://openalex.org/W2133501984","https://openalex.org/W2137118785","https://openalex.org/W2139206016","https://openalex.org/W2141128578","https://openalex.org/W2141424872","https://openalex.org/W2150163104","https://openalex.org/W2151487778","https://openalex.org/W2152237832","https://openalex.org/W2153478532","https://openalex.org/W2154472800","https://openalex.org/W2154568166","https://openalex.org/W2154712506","https://openalex.org/W2155676222","https://openalex.org/W2156363980","https://openalex.org/W2156555850","https://openalex.org/W2157156910","https://openalex.org/W2159861974","https://openalex.org/W2160266371","https://openalex.org/W2161949020","https://openalex.org/W2166649689","https://openalex.org/W2166670696","https://openalex.org/W2168610439","https://openalex.org/W2168684990","https://openalex.org/W2169529166","https://openalex.org/W2171892478","https://openalex.org/W2202569420","https://openalex.org/W2292788090","https://openalex.org/W2341116891","https://openalex.org/W2346138222","https://openalex.org/W2588514930","https://openalex.org/W7097257549","https://openalex.org/W7097749369"],"related_works":["https://openalex.org/W2063604885","https://openalex.org/W2596721143","https://openalex.org/W2134675555","https://openalex.org/W2037089986","https://openalex.org/W1804240129","https://openalex.org/W2161297782","https://openalex.org/W2789361467","https://openalex.org/W44275189","https://openalex.org/W2574507791","https://openalex.org/W2002764684"],"abstract_inverted_index":{"The":[0,40,82,107,153,197],"ability":[1,83],"of":[2,15,28,42,57,64,84,109,137,151,167,187,272,279],"embryonic":[3],"stem":[4],"cells":[5,227,237],"(ESCs)":[6],"to":[7,21,23,47,87,195,235,289,306],"self-renew":[8],"and":[9,66,98,121,134,142,212,281],"differentiate":[10],"into":[11],"a":[12,176],"wide":[13],"range":[14],"cell":[16,54],"types":[17],"has":[18,37],"encouraged":[19],"researchers":[20],"attempt":[22],"isolate":[24],"ESCs":[25],"from":[26,52,264],"embryos":[27,244,266],"domestic":[29],"species":[30],"for":[31,302],"the":[32,43,61,70,89,101,110,114,148,157,164,179,184,190,229,247,253,270,277,296,303],"past":[33],"two":[34],"decades.":[35],"Success":[36],"been":[38],"limited.":[39],"aim":[41],"current":[44],"study":[45,111],"was":[46,104,241],"investigate":[48],"whether":[49],"colonies":[50,231,262,283],"derived":[51],"inner":[53],"masses":[55],"(ICMs)":[56],"bovine":[58],"blastocysts":[59],"expressed":[60,124,146,162,208],"same":[62],"markers":[63],"pluripotency":[65,95,206,217],"candidate":[67,154],"genes":[68,155],"representing":[69,156],"various":[71,158],"signaling":[72,159,201],"pathways":[73,160,202],"as":[74,169,171],"those":[75],"found":[76],"in":[77,100,126,147,163,172,189,209,224,228,269,276],"human":[78],"or":[79],"mouse":[80],"ESCs.":[81],"selected":[85],"cytokines":[86],"maintain":[88],"major":[90,116],"transcription":[91,117],"factors":[92,118],"associated":[93,203],"with":[94,131,193,204,214],"(NANOG,":[96,119],"POU5F1":[97,120],"SOX2)":[99,122],"ICM":[102,191,210],"explants":[103,192,211],"also":[105,145],"tested.":[106,242],"results":[108,198],"showed":[112],"that":[113,200,213,245,295],"three":[115],"were":[123,129,144,161,232,256,284,299],"initially":[125],"culture":[127,133,168,297],"but":[128],"lost":[130],"continued":[132],"passaging.":[135,292],"Markers":[136],"differentiation":[138],"(BMP4,":[139],"HNF4,":[140],"NCAM,":[141],"CDX2)":[143],"initial":[149,165],"days":[150,166],"culture.":[152,311],"well":[170],"subsequent":[173],"passages.":[174],"Noggin,":[175],"cytokine":[177,215],"inhibiting":[178],"BMP4":[180],"pathway":[181],"successfully":[182],"up-regulated":[183],"relative":[185],"expression":[186],"NANOG":[188,254,304],"respect":[194],"controls.":[196],"indicate":[199],"regulating":[205],"are":[207],"supplementation":[216],"may":[218],"be":[219],"maintained.":[220],"An":[221],"alternate":[222],"approach":[223],"which":[225],"differentiating":[226],"primary":[230],"selectively":[233],"ablated":[234],"eradicate":[236],"secreting":[238],"pro-differentiation":[239],"signals":[240],"Bovine":[243],"carried":[246],"hygromycin":[248],"resistance":[249],"gene":[250],"driven":[251],"by":[252,258],"promoter":[255,305],"generated":[257,263],"SCNT.":[259],"Any":[260],"pluripotent":[261],"these":[265],"should":[267],"survive":[268],"presence":[271,278],"hygromycin.":[273],"When":[274],"cultured":[275],"Noggin":[280],"hygromycin,":[282],"generated;":[285],"however":[286],"they":[287],"failed":[288],"proliferate":[290],"on":[291],"This":[293],"suggests":[294],"conditions":[298],"not":[300],"optimal":[301],"remain":[307],"active":[308],"over":[309],"extended":[310]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
