{"id":"https://openalex.org/W1482229658","doi":null,"title":"Molecular engineering of high affinity T-cell receptors for bispecific therapeutics","display_name":"Molecular engineering of high affinity T-cell receptors for bispecific therapeutics","publication_year":2013,"publication_date":"2013-05-01","ids":{"openalex":"https://openalex.org/W1482229658","mag":"1482229658"},"language":"en","primary_location":{"id":"pmh:oai:https://orca.cardiff.ac.uk:47271","doi":null,"is_oa":true,"landing_page_url":"https://orca.cardiff.ac.uk/47271/1/2013LIDDYNphd.pdf","pdf_url":"https://orca.cardiff.ac.uk/47271/1/2013LIDDYNphd.pdf","source":{"id":"https://openalex.org/S4306401195","display_name":"ORCA Online Research @Cardiff (Cardiff University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79510175","host_organization_name":"Cardiff University","host_organization_lineage":["https://openalex.org/I79510175"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://orca.cardiff.ac.uk/47271/1/2013LIDDYNphd.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091470129","display_name":"Nathaniel Liddy","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Nathaniel Liddy","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5091470129"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.7559000253677368,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.7559000253677368,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.05959999933838844,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.046300001442432404,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/t-cell-receptor","display_name":"T cell receptor","score":0.8479999899864197},{"id":"https://openalex.org/keywords/bispecific-t-cell-engagers","display_name":"bispecific T-cell engagers","score":0.828000009059906},{"id":"https://openalex.org/keywords/hla-class-i","display_name":"HLA class I","score":0.6320000290870667},{"id":"https://openalex.org/keywords/melan-a-mart-1","display_name":"Melan-A/MART-1","score":0.8970000147819519},{"id":"https://openalex.org/keywords/gp100","display_name":"gp100","score":0.9959999918937683},{"id":"https://openalex.org/keywords/mage-a3","display_name":"MAGE-A3","score":1.0},{"id":"https://openalex.org/keywords/phage-display","display_name":"phage display","score":0.8320000171661377},{"id":"https://openalex.org/keywords/in-vitro-evolution","display_name":"in vitro evolution","score":0.656000018119812},{"id":"https://openalex.org/keywords/tcr-affinity","display_name":"TCR affinity","score":0.4090000092983246},{"id":"https://openalex.org/keywords/tumor-associated-antigens","display_name":"tumor-associated antigens","score":0.6570000052452087},{"id":"https://openalex.org/keywords/cancer-immunotherapy","display_name":"cancer immunotherapy","score":0.6579999923706055}],"concepts":[{"id":"https://openalex.org/C19317047","wikidata":"https://www.wikidata.org/wiki/Q412037","display_name":"T-cell receptor","level":4,"score":0.6980398893356323},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.6236172914505005},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.5485554337501526},{"id":"https://openalex.org/C188280979","wikidata":"https://www.wikidata.org/wiki/Q911125","display_name":"Human leukocyte antigen","level":3,"score":0.5356808304786682},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.4777492880821228},{"id":"https://openalex.org/C2780674031","wikidata":"https://www.wikidata.org/wiki/Q2012719","display_name":"Cancer immunotherapy","level":4,"score":0.450324684381485},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4384498596191406},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.43424293398857117},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4210616946220398},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3908649682998657},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.37096482515335083},{"id":"https://openalex.org/C2777701055","wikidata":"https://www.wikidata.org/wiki/Q1427096","display_name":"Immunotherapy","level":3,"score":0.26865577697753906},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2241164743900299},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2104579210281372},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.16683387756347656},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:https://orca.cardiff.ac.uk:47271","doi":null,"is_oa":true,"landing_page_url":"https://orca.cardiff.ac.uk/47271/1/2013LIDDYNphd.pdf","pdf_url":"https://orca.cardiff.ac.uk/47271/1/2013LIDDYNphd.pdf","source":{"id":"https://openalex.org/S4306401195","display_name":"ORCA Online Research @Cardiff (Cardiff University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79510175","host_organization_name":"Cardiff University","host_organization_lineage":["https://openalex.org/I79510175"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:oai:uilis.unsyiah.ac.id:35331","doi":null,"is_oa":true,"landing_page_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/35331","pdf_url":"http://orca.cf.ac.uk/47271/1/2013LIDDYNphd.pdf","source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:1482229658","doi":null,"is_oa":false,"landing_page_url":"http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.571741","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:https://orca.cardiff.ac.uk:47271","doi":null,"is_oa":true,"landing_page_url":"https://orca.cardiff.ac.uk/47271/1/2013LIDDYNphd.pdf","pdf_url":"https://orca.cardiff.ac.uk/47271/1/2013LIDDYNphd.pdf","source":{"id":"https://openalex.org/S4306401195","display_name":"ORCA Online Research @Cardiff (Cardiff University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79510175","host_organization_name":"Cardiff University","host_organization_lineage":["https://openalex.org/I79510175"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8999999761581421}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1482229658.pdf","grobid_xml":"https://content.openalex.org/works/W1482229658.grobid-xml"},"referenced_works_count":269,"referenced_works":["https://openalex.org/W19835385","https://openalex.org/W34502556","https://openalex.org/W65617872","https://openalex.org/W1485730287","https://openalex.org/W1489538658","https://openalex.org/W1498780407","https://openalex.org/W1502917753","https://openalex.org/W1513872850","https://openalex.org/W1516591549","https://openalex.org/W1517969155","https://openalex.org/W1519533633","https://openalex.org/W1519645049","https://openalex.org/W1531785323","https://openalex.org/W1533163141","https://openalex.org/W1533325200","https://openalex.org/W1548757996","https://openalex.org/W1549258100","https://openalex.org/W1568627452","https://openalex.org/W1588193878","https://openalex.org/W1594353637","https://openalex.org/W1599999513","https://openalex.org/W1601859667","https://openalex.org/W1603652226","https://openalex.org/W1606431180","https://openalex.org/W1607752827","https://openalex.org/W1706832386","https://openalex.org/W1780835683","https://openalex.org/W1822255413","https://openalex.org/W1840766718","https://openalex.org/W1852793186","https://openalex.org/W1855822212","https://openalex.org/W1898024522","https://openalex.org/W1914337318","https://openalex.org/W1928212615","https://openalex.org/W1963594770","https://openalex.org/W1964115378","https://openalex.org/W1964315730","https://openalex.org/W1964823826","https://openalex.org/W1965498454","https://openalex.org/W1965946471","https://openalex.org/W1967582433","https://openalex.org/W1969508492","https://openalex.org/W1969595441","https://openalex.org/W1971141213","https://openalex.org/W1972242350","https://openalex.org/W1973643740","https://openalex.org/W1975184547","https://openalex.org/W1977125553","https://openalex.org/W1977273255","https://openalex.org/W1978368856","https://openalex.org/W1979250687","https://openalex.org/W1980048816","https://openalex.org/W1980754944","https://openalex.org/W1982946346","https://openalex.org/W1984116510","https://openalex.org/W1984623187","https://openalex.org/W1986046751","https://openalex.org/W1986132642","https://openalex.org/W1986216579","https://openalex.org/W1986445948","https://openalex.org/W1988865436","https://openalex.org/W1989199118","https://openalex.org/W1989639228","https://openalex.org/W1989651201","https://openalex.org/W1990557050","https://openalex.org/W1992170081","https://openalex.org/W1992179441","https://openalex.org/W1992253391","https://openalex.org/W1992703130","https://openalex.org/W1992847016","https://openalex.org/W1993325062","https://openalex.org/W1993575340","https://openalex.org/W1994679552","https://openalex.org/W1994971739","https://openalex.org/W1995138538","https://openalex.org/W1995712039","https://openalex.org/W1996820981","https://openalex.org/W1997996516","https://openalex.org/W1998003752","https://openalex.org/W1998240361","https://openalex.org/W1998415250","https://openalex.org/W2000455214","https://openalex.org/W2000779612","https://openalex.org/W2002038445","https://openalex.org/W2003156803","https://openalex.org/W2003652884","https://openalex.org/W2004221867","https://openalex.org/W2005274005","https://openalex.org/W2005997381","https://openalex.org/W2006174330","https://openalex.org/W2006987944","https://openalex.org/W2007211891","https://openalex.org/W2007305532","https://openalex.org/W2008284182","https://openalex.org/W2008380006","https://openalex.org/W2009573319","https://openalex.org/W2009870709","https://openalex.org/W2010457947","https://openalex.org/W2010649076","https://openalex.org/W2011187661","https://openalex.org/W2011261569","https://openalex.org/W2012986020","https://openalex.org/W2014275356","https://openalex.org/W2015326650","https://openalex.org/W2015737099","https://openalex.org/W2016103121","https://openalex.org/W2021171990","https://openalex.org/W2021673674","https://openalex.org/W2022378336","https://openalex.org/W2022551121","https://openalex.org/W2024798162","https://openalex.org/W2025827008","https://openalex.org/W2027764644","https://openalex.org/W2030522563","https://openalex.org/W2031011895","https://openalex.org/W2034673683","https://openalex.org/W2035757181","https://openalex.org/W2037156781","https://openalex.org/W2037600719","https://openalex.org/W2041305950","https://openalex.org/W2041655031","https://openalex.org/W2041879875","https://openalex.org/W2042548343","https://openalex.org/W2042708573","https://openalex.org/W2043071551","https://openalex.org/W2043080692","https://openalex.org/W2043228552","https://openalex.org/W2044721777","https://openalex.org/W2045904095","https://openalex.org/W2046439007","https://openalex.org/W2047199560","https://openalex.org/W2047224828","https://openalex.org/W2048501505","https://openalex.org/W2049204549","https://openalex.org/W2049576685","https://openalex.org/W2051686043","https://openalex.org/W2052332203","https://openalex.org/W2052892134","https://openalex.org/W2053104174","https://openalex.org/W2054592480","https://openalex.org/W2055738243","https://openalex.org/W2057222401","https://openalex.org/W2058586537","https://openalex.org/W2060003702","https://openalex.org/W2060609324","https://openalex.org/W2061027548","https://openalex.org/W2061410538","https://openalex.org/W2061614901","https://openalex.org/W2061863874","https://openalex.org/W2063333925","https://openalex.org/W2065164093","https://openalex.org/W2065272367","https://openalex.org/W2066493864","https://openalex.org/W2066892610","https://openalex.org/W2068872497","https://openalex.org/W2068892677","https://openalex.org/W2068935062","https://openalex.org/W2070360032","https://openalex.org/W2070576765","https://openalex.org/W2071038107","https://openalex.org/W2071423255","https://openalex.org/W2071704671","https://openalex.org/W2072576950","https://openalex.org/W2074545819","https://openalex.org/W2074911859","https://openalex.org/W2075497712","https://openalex.org/W2075770210","https://openalex.org/W2077864494","https://openalex.org/W2078494443","https://openalex.org/W2080095423","https://openalex.org/W2081392849","https://openalex.org/W2081589596","https://openalex.org/W2082712562","https://openalex.org/W2083494119","https://openalex.org/W2084067246","https://openalex.org/W2085025732","https://openalex.org/W2085881249","https://openalex.org/W2086766201","https://openalex.org/W2087117158","https://openalex.org/W2087121403","https://openalex.org/W2088598907","https://openalex.org/W2089837686","https://openalex.org/W2090147596","https://openalex.org/W2090383712","https://openalex.org/W2090443272","https://openalex.org/W2091567124","https://openalex.org/W2091855294","https://openalex.org/W2092249806","https://openalex.org/W2092996181","https://openalex.org/W2093380070","https://openalex.org/W2094213517","https://openalex.org/W2095520403","https://openalex.org/W2096227747","https://openalex.org/W2096234619","https://openalex.org/W2096803586","https://openalex.org/W2096996532","https://openalex.org/W2098005365","https://openalex.org/W2098852858","https://openalex.org/W2099028937","https://openalex.org/W2099580557","https://openalex.org/W2100673335","https://openalex.org/W2101485657","https://openalex.org/W2101553545","https://openalex.org/W2101586035","https://openalex.org/W2103795331","https://openalex.org/W2106167249","https://openalex.org/W2106582884","https://openalex.org/W2108562020","https://openalex.org/W2110612592","https://openalex.org/W2110845720","https://openalex.org/W2113736279","https://openalex.org/W2114569569","https://openalex.org/W2115770884","https://openalex.org/W2117706207","https://openalex.org/W2117925216","https://openalex.org/W2118241155","https://openalex.org/W2119118456","https://openalex.org/W2121927641","https://openalex.org/W2122321884","https://openalex.org/W2122411218","https://openalex.org/W2124170576","https://openalex.org/W2125604001","https://openalex.org/W2126216921","https://openalex.org/W2127185415","https://openalex.org/W2127745456","https://openalex.org/W2127749132","https://openalex.org/W2128341317","https://openalex.org/W2129060396","https://openalex.org/W2129274679","https://openalex.org/W2130185552","https://openalex.org/W2131363383","https://openalex.org/W2132164705","https://openalex.org/W2134724359","https://openalex.org/W2136673346","https://openalex.org/W2137221978","https://openalex.org/W2140574666","https://openalex.org/W2142892753","https://openalex.org/W2144809277","https://openalex.org/W2144886557","https://openalex.org/W2146040535","https://openalex.org/W2146074801","https://openalex.org/W2146600597","https://openalex.org/W2146832462","https://openalex.org/W2148368831","https://openalex.org/W2148565862","https://openalex.org/W2149986918","https://openalex.org/W2151497263","https://openalex.org/W2151546364","https://openalex.org/W2152996957","https://openalex.org/W2154026406","https://openalex.org/W2154876959","https://openalex.org/W2155740919","https://openalex.org/W2156008881","https://openalex.org/W2156037313","https://openalex.org/W2157384708","https://openalex.org/W2157417177","https://openalex.org/W2157769714","https://openalex.org/W2158682956","https://openalex.org/W2158919479","https://openalex.org/W2159404298","https://openalex.org/W2159772201","https://openalex.org/W2160692925","https://openalex.org/W2160934060","https://openalex.org/W2165595227","https://openalex.org/W2167386937","https://openalex.org/W2167642721","https://openalex.org/W2171178977","https://openalex.org/W2200217481","https://openalex.org/W2318605753"],"related_works":["https://openalex.org/W2497992433","https://openalex.org/W2608257137","https://openalex.org/W2063184115","https://openalex.org/W4389016267","https://openalex.org/W4285736146","https://openalex.org/W2068872497","https://openalex.org/W2912462786","https://openalex.org/W3046293265","https://openalex.org/W2067050315","https://openalex.org/W2809984661"],"abstract_inverted_index":{"Cytotoxic":[0],"T":[1],"lymphocytes":[2],"are":[3,54,72,152,406],"able":[4],"to":[5,67,86,129,177,191,213,249,293,324,337,396,420],"identify":[6],"malignant":[7],"cells":[8],"by":[9,25,39,281,301],"scanning":[10],"for":[11,58,92,116,275,384,416],"aberrant":[12],"peptides":[13,100,144],"presented":[14,38],"on":[15,101,242,414],"cell":[16],"surface":[17,103,225,244,302],"human":[18],"leukocyte":[19],"antigen":[20,29,111,180,342],"(HLA)":[21],"Class":[22,41,139],"I":[23,42,140],"molecules":[24],"virtue":[26],"of":[27,48,90,98,104,114,125,158,245,259,295,332,348,366,401],"an":[28,159,360,380],"binding":[30,181,327,382],"receptor":[31,35],"called":[32],"the":[33,45,79,87,102,109,122,179,187,195,199,203,214,223,228,243,250,267,291,316,363,385,390,399,417,426],"T-cell":[34,65],"(TCR).":[36],"Peptides":[37],"HLA":[40,138,204,224],"complexes":[43],"represent":[44],"largest":[46],"array":[47],"tumour":[49],"associated":[50],"antigens":[51],"(TAAs)":[52],"and":[53,149,161,190,206,226,240,290,355,372],"therefore":[55],"ideal":[56],"targets":[57],"immunotherapeutic":[59],"reagents.":[60],"Cancer":[61],"patients":[62],"frequently":[63],"mount":[64],"responses":[66],"tumour-specific":[68],"antigens,":[69],"but":[70],"these":[71],"in":[73,83,202,260,320,436],"most":[74],"cases":[75],"ineffective":[76],"at":[77],"clearing":[78],"tumour.":[80],"This":[81],"is":[82,210,431],"part":[84],"due":[85],"low":[88],"affinity":[89,132,274,294,387,427],"TCRs":[91,115,133,151,271,279],"self-antigens":[93],"coupled":[94],"with":[95,222,272,343],"low-level":[96],"expression":[97],"target":[99,268],"cancer":[105],"cells.":[106],"To":[107],"harness":[108],"exquisite":[110],"recognition":[112],"property":[113],"use":[117],"as":[118,207,247,266,286],"potential":[119],"therapeutic":[120],"proteins,":[121],"principal":[123],"goal":[124],"this":[126,402,409],"thesis":[127],"was":[128,234,299,335],"generate":[130],"ultra-high":[131],"against":[134],"three":[135,168],"clinically":[136],"relevant":[137],"melanoma-specific":[141],"epitopes,":[142],"including":[143],"derived":[145],"from":[146],"Melan-A/MART-1(26-35),":[147],"gp100(280-288)":[148],"MAGE-A3(168-176).":[150],"membrane-bound":[153],"disulphide":[154],"(ds)-linked":[155],"heterodimers":[156],"consisting":[157],"alpha":[160],"a":[162,184,192,256,307,321,412,437],"beta":[163],"chain.":[164],"Each":[165,231],"chain":[166],"comprises":[167],"hypervariable":[169],"or":[170],"complementarity-determining":[171],"region":[172],"(CDR)":[173],"loops,":[174,189,197],"which":[175],"assemble":[176],"form":[178],"domains.":[182],"As":[183],"general":[185],"rule":[186],"CDR3":[188,215],"lesser":[193],"extent":[194],"CDR1":[196],"contact":[198],"peptide":[200],"bound":[201,229],"groove":[205],"such":[208],"specificity":[209,400],"largely":[211],"attributable":[212],"loops.":[216],"The":[217,329],"remaining":[218],"CDR":[219,232,298],"loops":[220],"interact":[221],"not":[227],"peptide.":[230],"loop":[233],"mutagenised":[235],"using":[236,263],"degenerative":[237],"NNK":[238],"oligonucleotides":[239],"expressed":[241],"bacteriophage":[246],"fusions":[248],"phage":[251,282,391],"coat":[252],"protein":[253],"pIII.":[254],"Through":[255],"Darwinian":[257],"process":[258],"vitro":[261],"evolution":[262],"pHLA":[264,276,341],"ligand":[265],"molecule,":[269],"mutated":[270,297,311,333],"improved":[273,345],"were":[277,284,313,394],"identified.":[278],"engineered":[280],"display":[283,392],"produced":[285],"soluble":[287],"ds-linked":[288],"proteins":[289],"contribution":[292],"each":[296],"measured":[300],"plasmon":[303],"resonance":[304],"(SPR).":[305],"Using":[306],"combinatorial":[308],"strategy,":[309],"individual":[310],"CDRs":[312,334],"spliced":[314],"into":[315],"same":[317],"TCR":[318,365,430],"molecule":[319],"stepwise":[322],"manner":[323],"further":[325],"increase":[326,361],"affinity.":[328],"final":[330],"combination":[331],"shown":[336],"bind":[338],"their":[339],"cognate":[340],"substantially":[344],"KD":[346],"values":[347],"18":[349],"pM":[350,353,357],"(Melan-A/MART-1(26-35)),":[351],"11":[352],"(gp100(280-288))":[354],"58":[356],"(MAGE-A3(168-176)),":[358],"representing":[359],"over":[362],"wild-type":[364],"approximately":[367],"1.8":[368],"million-fold,":[369],"1.7":[370],"million-fold":[371,374],"3.7":[373],"respectively.":[375],"In":[376],"addition,":[377],"having":[378],"discovered":[379],"off-target":[381],"profile":[383],"high":[386],"MAGE-A3":[388],"TCR,":[389],"methodologies":[393],"explored":[395],"\\n5":[397],"\\nreestablish":[398],"molecule.":[403],"These":[404],"results":[405],"significant":[407],"because":[408],"has":[410],"provided":[411],"platform":[413],"which,":[415],"first":[418],"time,":[419],"make":[421],"TCR-based":[422],"therapeutics.":[423],"For":[424],"example,":[425],"enhanced":[428],"gp100":[429],"currently":[432],"undergoing":[433],"clinical":[434],"evaluation":[435],"Phase":[438],"I/II":[439],"trial.":[440]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1}],"updated_date":"2026-08-31T07:19:18.363097","created_date":"2016-06-24T00:00:00"}