{"id":"https://openalex.org/W1481691650","doi":"https://doi.org/10.3389/fpls.2015.00411","title":"Factors influencing somatic embryogenesis, regeneration, and Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) cultivar TME14","display_name":"Factors influencing somatic embryogenesis, regeneration, and Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) cultivar TME14","publication_year":2015,"publication_date":"2015-06-10","ids":{"openalex":"https://openalex.org/W1481691650","doi":"https://doi.org/10.3389/fpls.2015.00411","mag":"1481691650","pmid":"https://pubmed.ncbi.nlm.nih.gov/26113851","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4461822"},"language":"en","primary_location":{"id":"doi:10.3389/fpls.2015.00411","doi":"https://doi.org/10.3389/fpls.2015.00411","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2015.00411","pdf_url":"https://www.frontiersin.org/articles/10.3389/fpls.2015.00411/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fpls.2015.00411/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070145295","display_name":"Evans N. Nyaboga","orcid":"https://orcid.org/0000-0002-9467-975X","observed_orcids":["https://orcid.org/0000-0002-9467-975X"]},"institutions":[{"id":"https://openalex.org/I4210111914","display_name":"International Institute of Tropical Agriculture","ror":"https://ror.org/01a0ymj74","country_code":"KE","type":"nonprofit","lineage":["https://openalex.org/I1286583668","https://openalex.org/I4210111914"]}],"countries":["KE"],"is_corresponding":false,"raw_author_name":"Evans N. Nyaboga","raw_affiliation_strings":["Bioscience Center, International Institute of Tropical Agriculture, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioscience Center, International Institute of Tropical Agriculture, Nairobi, Kenya","institution_ids":["https://openalex.org/I4210111914"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021049617","display_name":"Joshua M. Njiru","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210111914","display_name":"International Institute of Tropical Agriculture","ror":"https://ror.org/01a0ymj74","country_code":"KE","type":"nonprofit","lineage":["https://openalex.org/I1286583668","https://openalex.org/I4210111914"]}],"countries":["KE"],"is_corresponding":false,"raw_author_name":"Joshua M. Njiru","raw_affiliation_strings":["Bioscience Center, International Institute of Tropical Agriculture, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioscience Center, International Institute of Tropical Agriculture, Nairobi, Kenya","institution_ids":["https://openalex.org/I4210111914"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086283313","display_name":"Leena Tripathi","orcid":"https://orcid.org/0000-0001-5723-4981","observed_orcids":["https://orcid.org/0000-0001-5723-4981"]},"institutions":[{"id":"https://openalex.org/I4210111914","display_name":"International Institute of Tropical Agriculture","ror":"https://ror.org/01a0ymj74","country_code":"KE","type":"nonprofit","lineage":["https://openalex.org/I1286583668","https://openalex.org/I4210111914"]}],"countries":["KE"],"is_corresponding":true,"raw_author_name":"Leena Tripathi","raw_affiliation_strings":["Bioscience Center, International Institute of Tropical Agriculture, Nairobi, Kenya"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioscience Center, International Institute of Tropical Agriculture, Nairobi, Kenya","institution_ids":["https://openalex.org/I4210111914"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5086283313"],"corresponding_institution_ids":["https://openalex.org/I4210111914"],"apc_list":{"value":3150,"currency":"CHF","value_usd":3801},"apc_paid":{"value":3150,"currency":"CHF","value_usd":3801},"fwci":7.1672,"has_fulltext":true,"cited_by_count":57,"citation_normalized_percentile":{"value":0.96764166,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"6","issue":null,"first_page":"411","last_page":"411"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12305","display_name":"Cassava research and cyanide","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cassava","display_name":"cassava","score":0.9929999709129333},{"id":"https://openalex.org/keywords/somatic-embryogenesis","display_name":"somatic embryogenesis","score":0.8659999966621399},{"id":"https://openalex.org/keywords/agrobacterium-mediated-transformation","display_name":"Agrobacterium-mediated transformation","score":0.8730000257492065},{"id":"https://openalex.org/keywords/transformation-efficiency","display_name":"transformation efficiency","score":0.6669999957084656},{"id":"https://openalex.org/keywords/gusa-reporter-gene","display_name":"gusA reporter gene","score":0.6800000071525574},{"id":"https://openalex.org/keywords/genetically-modified-plants","display_name":"transgenic plants","score":0.675000011920929},{"id":"https://openalex.org/keywords/regeneration","display_name":"regeneration","score":0.3970000147819519},{"id":"https://openalex.org/keywords/dkw-medium","display_name":"DKW medium","score":0.6499999761581421},{"id":"https://openalex.org/keywords/bap","display_name":"BAP","score":0.492000013589859}],"concepts":[{"id":"https://openalex.org/C201468095","wikidata":"https://www.wikidata.org/wiki/Q7559239","display_name":"Somatic embryogenesis","level":4,"score":0.9096636772155762},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.8296947479248047},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.7423830032348633},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7304583191871643},{"id":"https://openalex.org/C9549007","wikidata":"https://www.wikidata.org/wiki/Q1072909","display_name":"Callus","level":2,"score":0.557875394821167},{"id":"https://openalex.org/C2777461220","wikidata":"https://www.wikidata.org/wiki/Q589029","display_name":"Germplasm","level":2,"score":0.55500727891922},{"id":"https://openalex.org/C178050479","wikidata":"https://www.wikidata.org/wiki/Q7834084","display_name":"Transformation efficiency","level":5,"score":0.5207203030586243},{"id":"https://openalex.org/C122172344","wikidata":"https://www.wikidata.org/wiki/Q1503477","display_name":"Genetically modified crops","level":4,"score":0.5110822916030884},{"id":"https://openalex.org/C197321923","wikidata":"https://www.wikidata.org/wiki/Q4886","display_name":"Cultivar","level":2,"score":0.48842138051986694},{"id":"https://openalex.org/C2781186746","wikidata":"https://www.wikidata.org/wiki/Q994697","display_name":"Plantlet","level":4,"score":0.4602166414260864},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.3951056897640228},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.36869221925735474},{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.32486552000045776},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.2994874119758606},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2543807327747345},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11404019594192505},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0}],"mesh":[],"locations_count":8,"locations":[{"id":"doi:10.3389/fpls.2015.00411","doi":"https://doi.org/10.3389/fpls.2015.00411","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2015.00411","pdf_url":"https://www.frontiersin.org/articles/10.3389/fpls.2015.00411/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3439204","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4461822","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:26113851","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26113851","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in plant science","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4461822","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC4461822/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Plant Sci","raw_type":"Text"},{"id":"pmh:oai:cgspace.cgiar.org:10568/74436","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/10568/74436","pdf_url":null,"source":{"id":"https://openalex.org/S4306402055","display_name":"CGSPace A Repository of Agricultural Research Outputs (Consultative Group for International Agricultural Research)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1286583668","host_organization_name":"CGIAR","host_organization_lineage":["https://openalex.org/I1286583668"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Nyaboga, E.N., Njiru, J.M., & Tripathi, L. (2015). Factors influencing somatic embryogenesis, regeneration and agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz) cultivar TME14. Frontiers in Plant Science, 6, 411.","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:d2757bb5d447457db040a02ad627af1f","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/d2757bb5d447457db040a02ad627af1f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Plant Science, Vol 6 (2015)","raw_type":"article"},{"id":"doi:10.60692/27dcd-wxt09","doi":"https://doi.org/10.60692/27dcd-wxt09","is_oa":true,"landing_page_url":"https://doi.org/10.60692/27dcd-wxt09","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},{"id":"doi:10.60692/h026e-4nd71","doi":"https://doi.org/10.60692/h026e-4nd71","is_oa":true,"landing_page_url":"https://doi.org/10.60692/h026e-4nd71","pdf_url":null,"source":{"id":"https://openalex.org/S7407051682","display_name":"Greater South Information System","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.3389/fpls.2015.00411","doi":"https://doi.org/10.3389/fpls.2015.00411","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2015.00411","pdf_url":"https://www.frontiersin.org/articles/10.3389/fpls.2015.00411/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.9208999872207642,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306115","display_name":"United States Agency for International Development","ror":"https://ror.org/01n6e6j62"},{"id":"https://openalex.org/F4320328548","display_name":"International Livestock Research Institute","ror":"https://ror.org/01jxjwb74"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1481691650.pdf","grobid_xml":"https://content.openalex.org/works/W1481691650.grobid-xml"},"referenced_works_count":45,"referenced_works":["https://openalex.org/W133562867","https://openalex.org/W235402724","https://openalex.org/W1510880140","https://openalex.org/W1530204343","https://openalex.org/W1574233575","https://openalex.org/W1969239589","https://openalex.org/W1972262510","https://openalex.org/W1972659721","https://openalex.org/W1976391864","https://openalex.org/W1977766787","https://openalex.org/W1980719911","https://openalex.org/W1984001443","https://openalex.org/W1986867666","https://openalex.org/W1992255794","https://openalex.org/W1994035663","https://openalex.org/W2015553172","https://openalex.org/W2018177712","https://openalex.org/W2024556021","https://openalex.org/W2029476089","https://openalex.org/W2039706808","https://openalex.org/W2042623986","https://openalex.org/W2045309336","https://openalex.org/W2045439285","https://openalex.org/W2056706019","https://openalex.org/W2073764507","https://openalex.org/W2078044364","https://openalex.org/W2078834701","https://openalex.org/W2082004162","https://openalex.org/W2087660830","https://openalex.org/W2093767719","https://openalex.org/W2105551788","https://openalex.org/W2110380226","https://openalex.org/W2123438922","https://openalex.org/W2124109750","https://openalex.org/W2125222336","https://openalex.org/W2127645147","https://openalex.org/W2137238549","https://openalex.org/W2138076054","https://openalex.org/W2153347515","https://openalex.org/W2158650493","https://openalex.org/W2291602915","https://openalex.org/W2469161413","https://openalex.org/W2472059134","https://openalex.org/W4231655847","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W4243345077","https://openalex.org/W1972659721","https://openalex.org/W2278424719","https://openalex.org/W1981066370","https://openalex.org/W2397369405","https://openalex.org/W3119928582","https://openalex.org/W2104337971","https://openalex.org/W2367707779","https://openalex.org/W1601861276","https://openalex.org/W4254630570"],"abstract_inverted_index":{"Routine":[0],"production":[1,46],"of":[2,5,20,47,57,61,74,81,93,100,105,121,124,126,167,180,195,210,216],"large":[3],"numbers":[4],"transgenic":[6,159,189,211],"plants":[7,212],"is":[8],"required":[9],"to":[10,25,54,83],"fully":[11],"exploit":[12],"advances":[13],"in":[14,41,117,183],"cassava":[15,37,142,228],"biotechnology":[16],"and":[17,78,86,119,145,188,193,203,231,240],"support":[18],"development":[19],"improved":[21],"germplasm":[22],"for":[23,35,141,237],"deployment":[24],"farmers.":[26],"This":[27,219],"article":[28],"describes":[29],"an":[30],"improved,":[31],"high-efficiency":[32],"transformation":[33,87,224,229],"protocol":[34,220],"recalcitrant":[36],"cultivar":[38,143],"TME14":[39,144],"preferred":[40],"Africa.":[42],"Factors":[43],"that":[44],"favor":[45],"friable":[48],"embryogenic":[49,63],"calli":[50],"(FEC)":[51],"were":[52,139,169,199],"found":[53],"be":[55],"use":[56,92],"DKW":[58],"medium,":[59],"crushing":[60],"organized":[62],"structures":[64],"(OES)":[65],"through":[66],"1-2":[67],"mm":[68],"sized":[69],"metal":[70],"wire":[71],"mesh,":[72],"washing":[73],"crushed":[75],"OES":[76],"tissues":[77],"short":[79],"exposure":[80],"tyrosine":[82],"somatic":[84,108,186],"embryos;":[85],"efficiency":[88,225],"was":[89],"enhanced":[90,223],"by":[91,201],"low":[94],"Agrobacterium":[95,148],"density":[96],"during":[97],"co-cultivation,":[98],"co-centrifugation":[99],"FEC":[101,138,168],"with":[102,114],"Agrobacterium,":[103],"germination":[104],"paramomycin":[106],"resistant":[107],"embryos":[109,128,187],"on":[110,129,171],"medium":[111],"containing":[112],"BAP":[113],"gradual":[115],"increase":[116],"concentration":[118],"variations":[120],"the":[122,135,152,178,196,214],"frequency":[123],"subculture":[125],"cotyledonary-stage":[127],"shoot":[130],"elongation":[131],"medium.":[132,173],"By":[133],"applying":[134],"optimized":[136],"parameters,":[137],"produced":[140],"transformed":[146,184],"using":[147],"strain":[149],"LBA4404":[150],"harboring":[151],"binary":[153],"vector":[154],"pCAMBIA2301.":[155],"About":[156],"70-80":[157],"independent":[158],"lines":[160],"per":[161],"ml":[162],"settled":[163],"cell":[164],"volume":[165],"(SCV)":[166],"regenerated":[170],"selective":[172],"Histochemical":[174],"GUS":[175],"assays":[176],"confirmed":[177,200,213],"expression":[179,215],"gusA":[181,197,217],"gene":[182,198],"calli,":[185],"plants.":[190],"The":[191],"presence":[192],"integration":[194],"PCR":[202],"Southern":[204],"blot":[205],"analysis,":[206],"respectively.":[207],"RT-PCR":[208],"analysis":[209],"gene.":[218],"demonstrates":[221],"significantly":[222],"over":[226],"existing":[227],"protocols":[230],"could":[232],"become":[233],"a":[234],"powerful":[235],"tool":[236],"functional":[238],"genomics":[239],"transferring":[241],"new":[242],"traits":[243],"into":[244],"cassava.":[245]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":11},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":6},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}