{"id":"https://openalex.org/W1479900350","doi":"https://doi.org/10.1016/s0021-9258(18)32535-3","title":"Purification of insulin receptor with full binding activity.","display_name":"Purification of insulin receptor with full binding activity.","publication_year":1983,"publication_date":"1983-04-01","ids":{"openalex":"https://openalex.org/W1479900350","doi":"https://doi.org/10.1016/s0021-9258(18)32535-3","mag":"1479900350","pmid":"https://pubmed.ncbi.nlm.nih.gov/6833291"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)32535-3","doi":"https://doi.org/10.1016/s0021-9258(18)32535-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)32535-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)32535-3","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029893522","display_name":"Yoko Fujita\u2010Yamaguchi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]},{"id":"https://openalex.org/I146500386","display_name":"Jichi Medical University","ror":"https://ror.org/010hz0g26","country_code":"JP","type":"education","lineage":["https://openalex.org/I146500386"]},{"id":"https://openalex.org/I4210110459","display_name":"City of Hope","ror":"https://ror.org/01z1vct10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"Y Fujita-Yamaguchi","raw_affiliation_strings":["From the Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 and","the +Jichi Medical School, Minamikawachi-machi, Tochigi, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 and","institution_ids":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]},{"raw_affiliation_string":"the +Jichi Medical School, Minamikawachi-machi, Tochigi, Japan","institution_ids":["https://openalex.org/I146500386"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069441381","display_name":"Sang\u2010Bong Choi","orcid":"https://orcid.org/0000-0001-6040-2472","observed_orcids":["https://orcid.org/0000-0001-6040-2472"]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]},{"id":"https://openalex.org/I146500386","display_name":"Jichi Medical University","ror":"https://ror.org/010hz0g26","country_code":"JP","type":"education","lineage":["https://openalex.org/I146500386"]},{"id":"https://openalex.org/I4210110459","display_name":"City of Hope","ror":"https://ror.org/01z1vct10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"S Choi","raw_affiliation_strings":["From the Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 and","the +Jichi Medical School, Minamikawachi-machi, Tochigi, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 and","institution_ids":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]},{"raw_affiliation_string":"the +Jichi Medical School, Minamikawachi-machi, Tochigi, Japan","institution_ids":["https://openalex.org/I146500386"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027097809","display_name":"Yujiro Sakamoto","orcid":"https://orcid.org/0000-0003-4531-2318","observed_orcids":["https://orcid.org/0000-0003-4531-2318"]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]},{"id":"https://openalex.org/I146500386","display_name":"Jichi Medical University","ror":"https://ror.org/010hz0g26","country_code":"JP","type":"education","lineage":["https://openalex.org/I146500386"]},{"id":"https://openalex.org/I4210110459","display_name":"City of Hope","ror":"https://ror.org/01z1vct10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"Y Sakamoto","raw_affiliation_strings":["From the Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 and","the +Jichi Medical School, Minamikawachi-machi, Tochigi, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 and","institution_ids":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]},{"raw_affiliation_string":"the +Jichi Medical School, Minamikawachi-machi, Tochigi, Japan","institution_ids":["https://openalex.org/I146500386"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084759217","display_name":"Koji Itakura","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1301076528","display_name":"City Of Hope National Medical Center","ror":"https://ror.org/00w6g5w60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528"]},{"id":"https://openalex.org/I146500386","display_name":"Jichi Medical University","ror":"https://ror.org/010hz0g26","country_code":"JP","type":"education","lineage":["https://openalex.org/I146500386"]},{"id":"https://openalex.org/I4210110459","display_name":"City of Hope","ror":"https://ror.org/01z1vct10","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"K Itakura","raw_affiliation_strings":["From the Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 and","the +Jichi Medical School, Minamikawachi-machi, Tochigi, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Molecular Genetics, City of Hope Research Institute, Duarte, California 91010 and","institution_ids":["https://openalex.org/I1301076528","https://openalex.org/I4210110459"]},{"raw_affiliation_string":"the +Jichi Medical School, Minamikawachi-machi, Tochigi, Japan","institution_ids":["https://openalex.org/I146500386"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":13.0878,"has_fulltext":false,"cited_by_count":273,"citation_normalized_percentile":{"value":0.99414656,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"258","issue":"8","first_page":"5045","last_page":"5049"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.25850000977516174,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.25850000977516174,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.20640000700950623,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.12250000238418579,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/insulin-receptor","display_name":"insulin receptor","score":1.0},{"id":"https://openalex.org/keywords/receptor-purification","display_name":"receptor purification","score":0.8790000081062317},{"id":"https://openalex.org/keywords/insulin-binding","display_name":"insulin binding","score":0.5189999938011169},{"id":"https://openalex.org/keywords/human-placenta","display_name":"human placenta","score":0.6959999799728394},{"id":"https://openalex.org/keywords/wheat-germ-agglutinin-affinity-chromatography","display_name":"wheat germ agglutinin affinity chromatography","score":0.8899999856948853},{"id":"https://openalex.org/keywords/urea-elution","display_name":"urea elution","score":0.5509999990463257},{"id":"https://openalex.org/keywords/receptor-stability","display_name":"receptor stability","score":0.40700000524520874},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.49900001287460327},{"id":"https://openalex.org/keywords/scatchard-analysis","display_name":"Scatchard analysis","score":0.6990000009536743},{"id":"https://openalex.org/keywords/specific-activity","display_name":"specific activity","score":0.4909999966621399}],"concepts":[{"id":"https://openalex.org/C112446052","wikidata":"https://www.wikidata.org/wiki/Q422579","display_name":"Insulin receptor","level":4,"score":0.5735654830932617},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.5251001119613647},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4881070554256439},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.43982255458831787},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.36218810081481934},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.26896432042121887},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.26556360721588135},{"id":"https://openalex.org/C2777391703","wikidata":"https://www.wikidata.org/wiki/Q1053470","display_name":"Insulin resistance","level":3,"score":0.1403234601020813}],"mesh":[{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010920","descriptor_name":"Placenta","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011247","descriptor_name":"Pregnancy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011972","descriptor_name":"Receptor, Insulin","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D011972","descriptor_name":"Receptor, Insulin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)32535-3","doi":"https://doi.org/10.1016/s0021-9258(18)32535-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)32535-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:6833291","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6833291","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)32535-3","doi":"https://doi.org/10.1016/s0021-9258(18)32535-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)32535-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W180238663","https://openalex.org/W1484115349","https://openalex.org/W1532436604","https://openalex.org/W1534556626","https://openalex.org/W1561797960","https://openalex.org/W1568118221","https://openalex.org/W1573534810","https://openalex.org/W1577543390","https://openalex.org/W1591826165","https://openalex.org/W1625660792","https://openalex.org/W1895505522","https://openalex.org/W1975236443","https://openalex.org/W1983649348","https://openalex.org/W1987446470","https://openalex.org/W1998571264","https://openalex.org/W2005370615","https://openalex.org/W2006302031","https://openalex.org/W2015515639","https://openalex.org/W2022961230","https://openalex.org/W2024504149","https://openalex.org/W2043390818","https://openalex.org/W2051573866","https://openalex.org/W2056311512","https://openalex.org/W2064121091","https://openalex.org/W2066210780","https://openalex.org/W2073691118","https://openalex.org/W2090784961","https://openalex.org/W2171118958"],"related_works":["https://openalex.org/W2066210780","https://openalex.org/W2322828857","https://openalex.org/W1591826165","https://openalex.org/W1696114473","https://openalex.org/W1975236443","https://openalex.org/W2770972393","https://openalex.org/W137414433","https://openalex.org/W1516864699","https://openalex.org/W2051573866","https://openalex.org/W2060326031"],"abstract_inverted_index":{"Insulin":[0],"receptor":[1,25,53,96,116],"was":[2,26,38,55],"purified":[3,95],"2400-fold":[4],"with":[5,70,85,102,120],"an":[6],"overall":[7],"yield":[8],"of":[9,106,111,127,134,140],"40%":[10],"from":[11,28,118],"human":[12],"placental":[13],"membranes":[14],"by":[15,155],"affinity":[16],"chromatography":[17],"on":[18],"wheat":[19],"germ":[20],"agglutinin-Sepharose":[21],"and":[22,40,46,75,80,89],"insulin-Sepharose.":[23],"The":[24,94],"eluted":[27,117],"insulin-Sepharose":[29,119],"using":[30,156],"mild":[31],"conditions,":[32],"eliminating":[33],"urea,":[34],"so":[35],"that":[36,51,144],"it":[37],"stable":[39],"retained":[41],"full":[42],"insulin-binding":[43],"activity.":[44],"Chromatofocusing":[45],"gel":[47,61],"filtration":[48],"analysis":[49],"indicated":[50],"the":[52,115,125,157],"preparation":[54],"apparently":[56],"pure.":[57],"Sodium":[58],"dodecyl":[59],"sulfate-polyacrylamide":[60],"electrophoresis":[62],"showed":[63,97],"three":[64],"high":[65],"molecular":[66],"weight":[67],"protein":[68,83],"bands":[69,84],"Mr":[71,86],"=":[72,87],"320,000,":[73],"300,000,":[74],"270,000":[76],"under":[77,91],"nonreducing":[78],"conditions":[79,123],"two":[81],"major":[82],"135,000":[88],"90,000":[90],"reducing":[92],"conditions.":[93,160],"a":[98,145],"curvilinear":[99],"Scatchard":[100],"plot":[101],"maximum":[103,131],"insulin":[104,132],"binding":[105,133],"28.5":[107],"micrograms":[108,137],"per":[109,138],"mg":[110,139],"protein.":[112,141],"In":[113],"comparison,":[114],"previously":[121],"used":[122],"in":[124,130,149],"presence":[126],"urea":[128],"resulted":[129],"only":[135],"6":[136],"This":[142],"indicates":[143],"4-to":[146],"5-fold":[147],"increase":[148],"specific":[150],"activity":[151],"can":[152],"be":[153],"obtained":[154],"new":[158],"elution":[159]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":3},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}