{"id":"https://openalex.org/W13988366","doi":null,"title":"Transport of tetracyclines through the bacterial cell membrane assayed by fluorescence: a study with susceptible and resistant strains of Staphylococcus aureus and Escherichia coli.","display_name":"Transport of tetracyclines through the bacterial cell membrane assayed by fluorescence: a study with susceptible and resistant strains of Staphylococcus aureus and Escherichia coli.","publication_year":1978,"publication_date":"1978-01-01","ids":{"openalex":"https://openalex.org/W13988366","mag":"13988366","pmid":"https://pubmed.ncbi.nlm.nih.gov/375035"},"language":"en","primary_location":{"id":"pmid:375035","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/375035","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbios","raw_type":"Comparative Study"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Z Samra","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Z Samra","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010573716","display_name":"J Steinmetz","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Krausz-Steinmetz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5087500554","display_name":"D Sompolinsky","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Sompolinsky","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.6981,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.69981632,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"21","issue":"83","first_page":"7","last_page":"21"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.41359999775886536,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.41359999775886536,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.17419999837875366,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.15150000154972076,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tetracycline","display_name":"tetracycline","score":0.9300000071525574},{"id":"https://openalex.org/keywords/fluorometric-assay","display_name":"fluorometric assay","score":0.5490000247955322},{"id":"https://openalex.org/keywords/staphylococcus-aureus","display_name":"Staphylococcus aureus","score":0.8650000095367432},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":1.0},{"id":"https://openalex.org/keywords/tetracycline-resistance","display_name":"tetracycline resistance","score":0.8569999933242798},{"id":"https://openalex.org/keywords/minocycline","display_name":"minocycline","score":0.9490000009536743},{"id":"https://openalex.org/keywords/doxycycline","display_name":"doxycycline","score":0.8330000042915344},{"id":"https://openalex.org/keywords/chlortetracycline","display_name":"chlortetracycline","score":0.8700000047683716},{"id":"https://openalex.org/keywords/demethylchlortetracycline","display_name":"demethylchlortetracycline","score":0.8650000095367432},{"id":"https://openalex.org/keywords/intracellular-drug-levels","display_name":"intracellular drug levels","score":0.5609999895095825}],"concepts":[{"id":"https://openalex.org/C2780923616","wikidata":"https://www.wikidata.org/wiki/Q193045","display_name":"Tetracycline","level":3,"score":0.9313141107559204},{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.7516330480575562},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6936225295066833},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6550024747848511},{"id":"https://openalex.org/C2777179404","wikidata":"https://www.wikidata.org/wiki/Q422442","display_name":"Doxycycline","level":3,"score":0.5468910336494446},{"id":"https://openalex.org/C2778275353","wikidata":"https://www.wikidata.org/wiki/Q415336","display_name":"Minocycline","level":3,"score":0.5464614033699036},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5099643468856812},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.46729663014411926},{"id":"https://openalex.org/C3019249092","wikidata":"https://www.wikidata.org/wiki/Q274493","display_name":"Antibacterial agent","level":3,"score":0.4664553701877594},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36781030893325806},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3239009380340576},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.29394036531448364},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.26464390754699707},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2589409649372101},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D001692","descriptor_name":"Biological Transport","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004352","descriptor_name":"Drug Resistance, Microbial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008274","descriptor_name":"Magnesium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013050","descriptor_name":"Spectrometry, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013754","descriptor_name":"Tetracyclines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:375035","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/375035","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbios","raw_type":"Comparative Study"},{"id":"mag:13988366","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/375035","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W173963474","https://openalex.org/W2023107028","https://openalex.org/W1782528560","https://openalex.org/W2090546547","https://openalex.org/W2132244862","https://openalex.org/W2155663431","https://openalex.org/W2036145273","https://openalex.org/W2040246934","https://openalex.org/W4416259151","https://openalex.org/W7096876010"],"abstract_inverted_index":{"The":[0,20,83],"fluorescence":[1,21,33,73,192,210],"of":[2,46,56,87,90,107,111,118,122,172,179,191,204,209,236],"12":[3],"tetracyclines":[4,26],"in":[5,63,131,162,202,220,225,238],"buffered":[6],"solutions":[7,117],"was":[8,40,50,65,92,103,194,228],"measured":[9],"by":[10,67,128,212],"excitation":[11],"at":[12,17,27,43],"400":[13],"nm":[14],"and":[15,37,141,182,215,241],"emission":[16],"520":[18],"nm.":[19],"varied":[22,135],"markedly":[23],"for":[24,80,94,149,197,250,255],"different":[25,81,139],"equivalent":[28],"concentrations.":[29],"Demethylchlortetracycline":[30],"exhibited":[31,211],"more":[32],"than":[34,196],"both":[35],"chlortetracycline":[36],"demthyltetracycline;":[38],"minocycline":[39,231],"virtually":[41],"non-fluorescent":[42],"the":[44,47,54,57,60,88,99,123,138,147,159,163,173,189,198,206,213,223,233],"conditions":[45],"study.":[48],"Fluorescence":[49],"highly":[51],"dependent":[52],"on":[53,101],"polarity":[55],"solvent;":[58],"when":[59],"buffer":[61],"solution":[62],"water":[64],"replaced":[66],"a":[68,108,168],"solvent":[69],"containing":[70],"50%":[71,150],"methanol,":[72],"increased":[74],"significantly,":[75],"but":[76,253],"to":[77,116,158,167,187,222,247],"various":[78],"degrees":[79],"tetracyclines.":[82],"most":[84],"striking":[85],"influence":[86,100],"addition":[89],"methanol":[91],"observed":[93,193],"doxycycline":[95],"(5-oxy":[96],"6-deoxy-tetracycline),":[97],"whereas":[98],"anhydrotetracycline":[102],"negligible.":[104],"When":[105,176],"suspensions":[106],"susceptible":[109,200,214],"strain":[110],"Staphylococcus":[112],"aureus":[113,181,240],"were":[114,185],"added":[115],"tetracyclines,":[119],"membrane":[120,234],"permeation":[121],"drugs":[124,140],"could":[125,142],"be":[126,144,156,248],"monitored":[127],"an":[129],"increase":[130,134],"fluorescence.":[132],"This":[133,154,244],"strikingly":[136],"with":[137,146],"not":[143,254],"correlated":[145],"concentrations":[148],"growth":[151],"inhibition":[152,245],"(Ki).":[153],"might":[155],"due":[157],"quantitative":[160,207],"variations":[161],"intracellular":[164],"level":[165],"corresponding":[166,199],"certain":[169],"external":[170],"concentration":[171],"respective":[174],"drug.":[175],"tetracycline-resistant":[177],"strains":[178,217],"S.":[180,239,251],"Escherichia":[183],"coli":[184],"exposed":[186],"tetracycline,":[188],"intensity":[190],"less":[195],"strains;":[201],"spite":[203],"this,":[205],"differences":[208,224],"resistant":[216],"seemed":[218],"slight":[219],"relation":[221],"susceptibility.":[226],"It":[227],"demonstrated":[229],"that":[230],"inhibits":[232],"transport":[235],"tetracycline":[237],"E.":[242,256],"coli.":[243,257],"seems":[246],"competitive":[249],"aureus,":[252]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}