{"id":"https://openalex.org/W138214879","doi":null,"title":"Construction and Screening of Custom Peptide Libraries Derived from Synthetic DNA Pools.","display_name":"Construction and Screening of Custom Peptide Libraries Derived from Synthetic DNA Pools.","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W138214879","mag":"138214879"},"language":"en","primary_location":{"id":"pmh:oai:deepblue.lib.umich.edu:2027.42/86451","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/2027.42/86451","pdf_url":"https://hdl.handle.net/2027.42/86451","source":{"id":"https://openalex.org/S4306400393","display_name":"Deep Blue (University of Michigan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I27837315","host_organization_name":"University of Michigan","host_organization_lineage":["https://openalex.org/I27837315"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://hdl.handle.net/2027.42/86451","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060993397","display_name":"Saadet Albayrak Guralp","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Saadet Albayrak","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5060993397"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.9169999957084656,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.9169999957084656,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":0.07100000232458115,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.005499999970197678,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/peptide-libraries","display_name":"peptide libraries","score":0.675000011920929},{"id":"https://openalex.org/keywords/reverse-translation","display_name":"reverse translation","score":0.6809999942779541},{"id":"https://openalex.org/keywords/library-complexity","display_name":"library complexity","score":0.6240000128746033},{"id":"https://openalex.org/keywords/antimicrobial-peptides","display_name":"antimicrobial peptides","score":0.8740000128746033},{"id":"https://openalex.org/keywords/pediocin-pa-1","display_name":"pediocin PA-1","score":0.9480000138282776}],"concepts":[{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.560877799987793},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5099860429763794},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.47020941972732544},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3921331465244293},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.3821232318878174},{"id":"https://openalex.org/C2522767166","wikidata":"https://www.wikidata.org/wiki/Q2374463","display_name":"Data science","level":1,"score":0.35558587312698364},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34185752272605896},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2471906542778015},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19030308723449707}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:deepblue.lib.umich.edu:2027.42/86451","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/2027.42/86451","pdf_url":"https://hdl.handle.net/2027.42/86451","source":{"id":"https://openalex.org/S4306400393","display_name":"Deep Blue (University of Michigan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I27837315","host_organization_name":"University of Michigan","host_organization_lineage":["https://openalex.org/I27837315"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:138214879","doi":null,"is_oa":false,"landing_page_url":"https://deepblue.lib.umich.edu/handle/2027.42/86451","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:deepblue.lib.umich.edu:2027.42/86451","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/2027.42/86451","pdf_url":"https://hdl.handle.net/2027.42/86451","source":{"id":"https://openalex.org/S4306400393","display_name":"Deep Blue (University of Michigan)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I27837315","host_organization_name":"University of Michigan","host_organization_lineage":["https://openalex.org/I27837315"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W138214879.pdf","grobid_xml":"https://content.openalex.org/works/W138214879.grobid-xml"},"referenced_works_count":112,"referenced_works":["https://openalex.org/W70790455","https://openalex.org/W185146309","https://openalex.org/W186534562","https://openalex.org/W204267987","https://openalex.org/W207966931","https://openalex.org/W1243605242","https://openalex.org/W1516030996","https://openalex.org/W1517594500","https://openalex.org/W1526796829","https://openalex.org/W1546049076","https://openalex.org/W1546341583","https://openalex.org/W1573827588","https://openalex.org/W1606435569","https://openalex.org/W1638851988","https://openalex.org/W1752332248","https://openalex.org/W1955781683","https://openalex.org/W1965414380","https://openalex.org/W1968957319","https://openalex.org/W1969711244","https://openalex.org/W1970743383","https://openalex.org/W1970847599","https://openalex.org/W1971756895","https://openalex.org/W1973770807","https://openalex.org/W1978206537","https://openalex.org/W1979963523","https://openalex.org/W1980889490","https://openalex.org/W1981511134","https://openalex.org/W1981774753","https://openalex.org/W1984683656","https://openalex.org/W1989128313","https://openalex.org/W1989998611","https://openalex.org/W1991818457","https://openalex.org/W1995712039","https://openalex.org/W2000456081","https://openalex.org/W2003048585","https://openalex.org/W2004674004","https://openalex.org/W2007527881","https://openalex.org/W2010560388","https://openalex.org/W2010649076","https://openalex.org/W2011513747","https://openalex.org/W2015968948","https://openalex.org/W2018590530","https://openalex.org/W2022257442","https://openalex.org/W2023606718","https://openalex.org/W2023893656","https://openalex.org/W2026482455","https://openalex.org/W2027420997","https://openalex.org/W2028821719","https://openalex.org/W2029023177","https://openalex.org/W2030711123","https://openalex.org/W2031343644","https://openalex.org/W2037658092","https://openalex.org/W2038583114","https://openalex.org/W2039707307","https://openalex.org/W2040311886","https://openalex.org/W2045814624","https://openalex.org/W2047015012","https://openalex.org/W2048805199","https://openalex.org/W2051068713","https://openalex.org/W2052607686","https://openalex.org/W2054602599","https://openalex.org/W2058268622","https://openalex.org/W2059464705","https://openalex.org/W2061784494","https://openalex.org/W2061910359","https://openalex.org/W2066837313","https://openalex.org/W2067658680","https://openalex.org/W2070762622","https://openalex.org/W2071086763","https://openalex.org/W2072499238","https://openalex.org/W2080434522","https://openalex.org/W2087274035","https://openalex.org/W2090081965","https://openalex.org/W2092846883","https://openalex.org/W2094954825","https://openalex.org/W2095646187","https://openalex.org/W2097098774","https://openalex.org/W2100621506","https://openalex.org/W2104205339","https://openalex.org/W2105018887","https://openalex.org/W2108971604","https://openalex.org/W2109577485","https://openalex.org/W2110495869","https://openalex.org/W2112187645","https://openalex.org/W2114496961","https://openalex.org/W2114522205","https://openalex.org/W2115749923","https://openalex.org/W2117896192","https://openalex.org/W2120348757","https://openalex.org/W2120357935","https://openalex.org/W2122421045","https://openalex.org/W2122500226","https://openalex.org/W2122634856","https://openalex.org/W2124903080","https://openalex.org/W2128817262","https://openalex.org/W2135821812","https://openalex.org/W2137488386","https://openalex.org/W2140361413","https://openalex.org/W2143691706","https://openalex.org/W2144931881","https://openalex.org/W2149464124","https://openalex.org/W2151633191","https://openalex.org/W2152468191","https://openalex.org/W2160778701","https://openalex.org/W2160780417","https://openalex.org/W2161273461","https://openalex.org/W2162849538","https://openalex.org/W2164618276","https://openalex.org/W2167534673","https://openalex.org/W2263691456","https://openalex.org/W2286365641","https://openalex.org/W2804007336"],"related_works":["https://openalex.org/W2035292120","https://openalex.org/W4223650374","https://openalex.org/W2041719513","https://openalex.org/W2766655176","https://openalex.org/W2947857374","https://openalex.org/W2762343190","https://openalex.org/W2176480329","https://openalex.org/W133941726","https://openalex.org/W4313270582","https://openalex.org/W1992913603"],"abstract_inverted_index":{"The":[0],"latest":[1],"technologic":[2],"advancements":[3],"in":[4,16,30,46,70,89,169],"chemical":[5],"synthesis":[6,50],"of":[7,61,79,99,107,125,145,159,165,171,212,219],"DNA":[8,86],"and":[9,35,93,116,130,162,223],"peptides":[10,80,147,173,197,214],"led":[11],"to":[12,33,112,118,143,189,193,215],"a":[13,55,90],"tremendous":[14],"increase":[15],"protein":[17],"engineering":[18],"research.":[19],"High":[20],"throughput,":[21],"economically":[22],"feasible":[23],"as":[24,26,137,177,179],"well":[25,178],"design-flexible":[27],"platforms":[28],"are":[29,81],"much":[31],"demand":[32],"construct":[34],"screen":[36],"large":[37,62],"peptide":[38,65,100],"libraries":[39,66,155,167,192],"for":[40,58,85,96,134,157,207],"numerous":[41],"applications.":[42],"By":[43],"combining":[44],"light-directed":[45],"situ":[47],"parallel":[48],"oligonucleotide":[49],"with":[51,102,174],"biological":[52,221],"expression":[53,139],"systems,":[54,127],"novel":[56],"method":[57,203],"the":[59,97],"construction":[60],"custom":[63],"designed":[64],"has":[67,148],"been":[68,149],"developed":[69],"this":[71,74,108,202],"study.":[72],"In":[73],"method,":[75],"fully":[76],"defined":[77],"collections":[78],"reverse-translated":[82],"into":[83],"oligonucleotides":[84],"synthesis,":[87],"expressed":[88],"cellular":[91,126],"host,":[92],"then":[94],"screened":[95],"selection":[98],"analogs":[101],"desired":[103],"properties.":[104],"Each":[105],"step":[106],"process,":[109],"from":[110,210],"design":[111],"screening,":[113],"was":[114],"examined":[115],"optimized":[117],"maintain":[119],"initial":[120],"library":[121],"complexity.":[122],"A":[123],"variety":[124],"both":[128],"eukaryotic":[129],"prokaryotic,":[131],"were":[132],"investigated":[133],"their":[135,220],"potential":[136,206],"an":[138],"host.":[140],"An":[141],"application":[142],"discovery":[144,211],"antimicrobial":[146],"demonstrated":[150],"by":[151],"constructing":[152],"two":[153],"mutant":[154,172],"coding":[156],"derivatives":[158],"Pediocin":[160],"PA-1":[161],"Plantaricin-423.":[163],"Screening":[164],"these":[166,196],"resulted":[168],"identification":[170],"greater":[175],"activities":[176],"substantial":[180],"knowledge":[181],"on":[182],"sequence-related":[183],"activity":[184],"that":[185],"can":[186],"be":[187],"used":[188],"generate":[190],"subsequent":[191],"further":[194],"optimize":[195],"against":[198],"selected":[199],"targets.":[200],"Therefore,":[201],"presents":[204],"great":[205],"countless":[208],"applications":[209],"therapeutic":[213],"gaining":[216],"fundamental":[217],"understanding":[218],"function":[222],"characteristics.":[224]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}
