{"id":"https://openalex.org/W136579853","doi":"https://doi.org/10.1016/s0021-9258(18)37947-x","title":"The chemical basis for interfacial activation of monomeric phospholipases A2. Autocatalytic derivatization of the enzyme by acyl transfer from substrate.","display_name":"The chemical basis for interfacial activation of monomeric phospholipases A2. Autocatalytic derivatization of the enzyme by acyl transfer from substrate.","publication_year":1988,"publication_date":"1988-08-01","ids":{"openalex":"https://openalex.org/W136579853","doi":"https://doi.org/10.1016/s0021-9258(18)37947-x","mag":"136579853","pmid":"https://pubmed.ncbi.nlm.nih.gov/3403524"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)37947-x","doi":"https://doi.org/10.1016/s0021-9258(18)37947-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)37947-x","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)37947-x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5008657856","display_name":"Wonhwa Cho","orcid":"https://orcid.org/0000-0003-1410-2481","observed_orcids":["https://orcid.org/0000-0003-1410-2481"]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W Cho","raw_affiliation_strings":["Department of Chemistry, University of Chicago, Illinois 60637"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Chicago, Illinois 60637","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070455969","display_name":"Alfredo G. Tomasselli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A G Tomasselli","raw_affiliation_strings":["Department of Chemistry, University of Chicago, Illinois 60637"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Chicago, Illinois 60637","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016822525","display_name":"Robert L. Heinrikson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R L Heinrikson","raw_affiliation_strings":["Department of Chemistry, University of Chicago, Illinois 60637"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Chicago, Illinois 60637","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018346037","display_name":"Ferenc J. K\u00e9zdy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F J K\u00e9zdy","raw_affiliation_strings":["Department of Chemistry, University of Chicago, Illinois 60637"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Chicago, Illinois 60637","institution_ids":["https://openalex.org/I40347166"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I40347166"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":9.3087,"has_fulltext":false,"cited_by_count":83,"citation_normalized_percentile":{"value":0.98441105,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"263","issue":"23","first_page":"11237","last_page":"11241"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11353","display_name":"Venomous Animal Envenomation and Studies","score":0.9014000296592712,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11353","display_name":"Venomous Animal Envenomation and Studies","score":0.9014000296592712,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.0284000001847744,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.026499999687075615,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phospholipase-a2","display_name":"phospholipase A2","score":0.9929999709129333},{"id":"https://openalex.org/keywords/interfacial-activation","display_name":"interfacial activation","score":0.9860000014305115},{"id":"https://openalex.org/keywords/enzyme-dimerization","display_name":"enzyme dimerization","score":0.9399999976158142},{"id":"https://openalex.org/keywords/agkistrodon-contortrix-contortrix","display_name":"Agkistrodon contortrix contortrix","score":0.9679999947547913},{"id":"https://openalex.org/keywords/crotalus-atrox","display_name":"Crotalus atrox","score":0.949999988079071},{"id":"https://openalex.org/keywords/lysine-acylation","display_name":"lysine acylation","score":0.6669999957084656},{"id":"https://openalex.org/keywords/lag-phase","display_name":"lag phase","score":0.5529999732971191},{"id":"https://openalex.org/keywords/phospholipid-hydrolysis","display_name":"phospholipid hydrolysis","score":0.6309999823570251},{"id":"https://openalex.org/keywords/snake-venom-enzymes","display_name":"snake venom enzymes","score":0.5099999904632568},{"id":"https://openalex.org/keywords/porcine-pancreatic-phospholipase-a2","display_name":"porcine pancreatic phospholipase A2","score":0.7599999904632568}],"concepts":[{"id":"https://openalex.org/C37263863","wikidata":"https://www.wikidata.org/wiki/Q786897","display_name":"Autocatalysis","level":3,"score":0.8217124938964844},{"id":"https://openalex.org/C2776923573","wikidata":"https://www.wikidata.org/wiki/Q2294884","display_name":"Derivatization","level":3,"score":0.7067484855651855},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.675540566444397},{"id":"https://openalex.org/C166940927","wikidata":"https://www.wikidata.org/wiki/Q178827","display_name":"Monomer","level":3,"score":0.6551892757415771},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6226327419281006},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5914944410324097},{"id":"https://openalex.org/C12927208","wikidata":"https://www.wikidata.org/wiki/Q210430","display_name":"Phospholipase","level":3,"score":0.44976454973220825},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.35755306482315063},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.351828932762146},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3284892439842224},{"id":"https://openalex.org/C42360764","wikidata":"https://www.wikidata.org/wiki/Q83588","display_name":"Chemical engineering","level":1,"score":0.325093150138855},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.32042038440704346},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.1805354356765747},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.06941977143287659},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.0},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004789","descriptor_name":"Enzyme Activation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010179","descriptor_name":"Pancreas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D010740","descriptor_name":"Phospholipases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010741","descriptor_name":"Phospholipases A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012911","descriptor_name":"Snakes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D054467","descriptor_name":"Phospholipases A2","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)37947-x","doi":"https://doi.org/10.1016/s0021-9258(18)37947-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)37947-x","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:3403524","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3403524","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Comparative Study"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)37947-x","doi":"https://doi.org/10.1016/s0021-9258(18)37947-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)37947-x","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation","score":0.8100000023841858}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1359521170","display_name":null,"funder_award_id":"HL-15062","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G6111167016","display_name":null,"funder_award_id":"HL18577","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W42098258","https://openalex.org/W648947995","https://openalex.org/W1001724175","https://openalex.org/W1494762484","https://openalex.org/W1501888177","https://openalex.org/W1512725913","https://openalex.org/W1522428977","https://openalex.org/W1523847362","https://openalex.org/W1533793715","https://openalex.org/W1590150548","https://openalex.org/W1849137810","https://openalex.org/W1983247836","https://openalex.org/W2007647806","https://openalex.org/W2017152618","https://openalex.org/W2039188502","https://openalex.org/W2044397167","https://openalex.org/W2051803128","https://openalex.org/W2054774711","https://openalex.org/W2080852251","https://openalex.org/W2343393806","https://openalex.org/W4211175188","https://openalex.org/W4251413262"],"related_works":["https://openalex.org/W1566192162","https://openalex.org/W2014344423","https://openalex.org/W2046609888","https://openalex.org/W1981995490","https://openalex.org/W1601534342","https://openalex.org/W1606585991","https://openalex.org/W2101598345","https://openalex.org/W2169082902","https://openalex.org/W2087146799","https://openalex.org/W2069253150"],"abstract_inverted_index":{"A":[0],"basic":[1],"monomeric":[2,81,129,207],"phospholipase":[3,146,159],"A2":[4,59,83,160],"from":[5,84,88,162],"the":[6,9,21,42,47,56,80,89,100,105,120,140,145,170,195,201,213],"venom":[7,90,165],"of":[8,23,25,46,74,91,99,111,139,152,172,203,222],"American":[10],"water":[11],"moccasin,":[12],"Agkistrodon":[13,92],"piscivorus":[14],"piscivorus,":[15],"undergoes":[16],"Ca2+-dependent,":[17],"autocatalytic":[18],"acylation":[19,143,191],"during":[20],"course":[22],"hydrolysis":[24,171,202],"both":[26,51],"model":[27],"and":[28,39,49,87,109,223],"natural":[29],"phospholipid":[30],"substrates.":[31],"Acylation":[32,113],"occurs":[33],"at":[34],"2":[35],"lysine":[36],"residues,":[37],"Lys-7":[38],"Lys-10,":[40],"in":[41,63,169,200],"NH2-terminal":[43],"alpha-helical":[44],"segment":[45],"enzyme,":[48],"when":[50],"positions":[52],"are":[53],"fully":[54,69],"derivatized,":[55],"stable":[57],"bisacylphospholipase":[58],"becomes":[60],"a":[61,131,153,216],"dimer":[62,161],"solution.":[64],"The":[65,156],"acylated":[66],"enzyme":[67,141],"is":[68,192],"activated":[70],"toward":[71],"monomolecular":[72],"layers":[73],"lecithins.":[75],"Similar":[76],"studies":[77],"applied":[78],"to":[79,118,134],"phospholipases":[82,227],"porcine":[85],"pancreas":[86],"contortrix":[93,94],"also":[95],"showed":[96],"irreversible":[97],"activation":[98,221],"enzymes":[101,117],"by":[102,142,206,226],"substrate":[103,179],"with":[104,127,190],"same":[106],"kinetic":[107],"consequences":[108],"formation":[110,151],"dimers.":[112],"thus":[114],"enables":[115],"these":[116],"overcome":[119],"lag":[121,168],"period":[122],"observed":[123],"under":[124],"such":[125],"conditions":[126],"native":[128],"phospholipases,":[130],"phenomenon":[132],"referred":[133],"as":[135],"interfacial":[136,148,220,224],"activation.":[137],"Activation":[138],"potentiates":[144],"for":[147,194,212,219],"recognition":[149,225],"via":[150],"dimeric":[154],"enzyme.":[155],"naturally":[157],"occurring":[158],"Crotalus":[163],"atrox":[164],"displays":[166],"no":[167],"lecithin":[173],"monolayers":[174],"nor":[175],"does":[176],"it":[177],"undergo":[178],"level":[180],"acylation.":[181],"These":[182],"facts":[183],"support":[184],"our":[185],"proposal":[186],"that":[187],"dimerization":[188],"concomitant":[189],"responsible":[193],"large":[196],"rate":[197],"enhancements":[198],"seen":[199],"aggregated":[204],"phospholipids":[205],"phospholipases.":[208],"Our":[209],"findings":[210],"demonstrate":[211],"first":[214],"time":[215],"chemical":[217],"mechanism":[218],"A2.":[228]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}