{"id":"https://openalex.org/W135873629","doi":"https://doi.org/10.1182/blood.v122.21.4290.4290","title":"Improved Igh-Based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR In MCL and MM","display_name":"Improved Igh-Based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR In MCL and MM","publication_year":2013,"publication_date":"2013-11-15","ids":{"openalex":"https://openalex.org/W135873629","doi":"https://doi.org/10.1182/blood.v122.21.4290.4290","mag":"135873629"},"language":"en","primary_location":{"id":"doi:10.1182/blood.v122.21.4290.4290","doi":"https://doi.org/10.1182/blood.v122.21.4290.4290","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v122.21.4290.4290","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056141793","display_name":"Daniela Drandi","orcid":"https://orcid.org/0000-0003-1825-1206","observed_orcids":["https://orcid.org/0000-0003-1825-1206"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Daniela Drandi","raw_affiliation_strings":["Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108524714","display_name":"Lenka Kubiczkov\u00e1","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I21449261","display_name":"Masaryk University","ror":"https://ror.org/02j46qs45","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21449261"]}],"countries":["CZ"],"is_corresponding":true,"raw_author_name":"Lenka Kubiczkov\u00e0","raw_affiliation_strings":["Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic,","institution_ids":["https://openalex.org/I21449261"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016419122","display_name":"Nadia Dani","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Nadia Dani","raw_affiliation_strings":["Bioclarma, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioclarma, Torino, Italy,","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012645361","display_name":"Simone Ferrero","orcid":"https://orcid.org/0000-0002-9711-1502","observed_orcids":["https://orcid.org/0000-0002-9711-1502"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Simone Ferrero","raw_affiliation_strings":["Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036916107","display_name":"Luigia Monitillo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Luigia Monitillo","raw_affiliation_strings":["University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079136601","display_name":"Barbara Mantoan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Barbara Mantoan","raw_affiliation_strings":["University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053891408","display_name":"Elisa Genuardi","orcid":"https://orcid.org/0000-0002-9624-0337","observed_orcids":["https://orcid.org/0000-0002-9624-0337"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Elisa Genuardi","raw_affiliation_strings":["Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045333278","display_name":"Daniela Barbero","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Daniela Barbero","raw_affiliation_strings":["University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055456835","display_name":"Manuela Gambella","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Manuela Gambella","raw_affiliation_strings":["University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103378970","display_name":"Davide Barberio","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Davide Barberio","raw_affiliation_strings":["Bioclarma, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioclarma, Torino, Italy,","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016042168","display_name":"Paola Ghione","orcid":"https://orcid.org/0000-0001-6986-7954","observed_orcids":["https://orcid.org/0000-0001-6986-7954"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Paola Ghione","raw_affiliation_strings":["University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010320947","display_name":"Guglielmo Guarona","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Guglielmo Guarona","raw_affiliation_strings":["Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080278478","display_name":"Elona Saraci","orcid":"https://orcid.org/0000-0002-2275-9654","observed_orcids":["https://orcid.org/0000-0002-2275-9654"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Elona Saraci","raw_affiliation_strings":["University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007558343","display_name":"Paola Omed\u00e8","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210097265","display_name":"Azienda Ospedaliero Universitaria San Giovanni Battista","ror":"https://ror.org/00nrtez23","country_code":"IT","type":"healthcare","lineage":["https://openalex.org/I4210097265"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Paola Omed\u00e8","raw_affiliation_strings":["Division of Hematology, A.O.U. S.Giovanni Battista, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Hematology, A.O.U. S.Giovanni Battista, Torino, Italy,","institution_ids":["https://openalex.org/I4210097265"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087781254","display_name":"Roberto Passera","orcid":"https://orcid.org/0000-0003-3372-2248","observed_orcids":["https://orcid.org/0000-0003-3372-2248"]},"institutions":[{"id":"https://openalex.org/I4210097265","display_name":"Azienda Ospedaliero Universitaria San Giovanni Battista","ror":"https://ror.org/00nrtez23","country_code":"IT","type":"healthcare","lineage":["https://openalex.org/I4210097265"]},{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Roberto Passera","raw_affiliation_strings":["Division of Nuclear Medicine, A.O.U. San Giovanni Battista, Statistical Consultant, University of Torino, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Nuclear Medicine, A.O.U. San Giovanni Battista, Statistical Consultant, University of Torino, Torino, Italy,","institution_ids":["https://openalex.org/I4210097265","https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090512266","display_name":"Roman H\u00e1jek","orcid":"https://orcid.org/0000-0001-6955-6267","observed_orcids":["https://orcid.org/0000-0001-6955-6267"]},"institutions":[{"id":"https://openalex.org/I187293425","display_name":"University of Ostrava","ror":"https://ror.org/00pyqav47","country_code":"CZ","type":"education","lineage":["https://openalex.org/I187293425"]},{"id":"https://openalex.org/I4210088935","display_name":"University Hospital Ostrava","ror":"https://ror.org/00a6yph09","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210088935"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Roman Hajek","raw_affiliation_strings":["Dept. of Hematooncology, University Hospital Ostrava and Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Hematooncology, University Hospital Ostrava and Faculty of Medicine, University of Ostrava, Ostrava, Czech Republic,","institution_ids":["https://openalex.org/I187293425","https://openalex.org/I4210088935"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051021130","display_name":"Sergio Cortelazzo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210091033","display_name":"Ospedale di Bolzano","ror":"https://ror.org/00cmk4n56","country_code":"IT","type":"healthcare","lineage":["https://openalex.org/I4210091033"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Sergio Cortelazzo","raw_affiliation_strings":["Hematology department, S. Maurizio Regional Hospital, Bolzano, Bozen, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hematology department, S. Maurizio Regional Hospital, Bolzano, Bozen, Italy,","institution_ids":["https://openalex.org/I4210091033"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082545947","display_name":"Antonio Palumbo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Antonio Palumbo","raw_affiliation_strings":["Myeloma Unit, Division of Hematology, University of Torino, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Myeloma Unit, Division of Hematology, University of Torino, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018139001","display_name":"Mario Boccadoro","orcid":"https://orcid.org/0000-0001-8130-5209","observed_orcids":["https://orcid.org/0000-0001-8130-5209"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Mario Boccadoro","raw_affiliation_strings":["Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Hematology, Department of Molecular Biotechnologies and Health Sciences, University of Torino, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006640724","display_name":"Giorgio Inghirami","orcid":"https://orcid.org/0000-0001-5566-0864","observed_orcids":["https://orcid.org/0000-0001-5566-0864","https://orcid.org/0000-0002-5298-6516"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Giorgio Inghirami","raw_affiliation_strings":["Department of Pathology and Center for Experimental Research and Medical Studies (CeRMS), University of Turin, Turin, Italy, Turin, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology and Center for Experimental Research and Medical Studies (CeRMS), University of Turin, Turin, Italy, Turin, Italy","institution_ids":["https://openalex.org/I55143463"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5076353357","display_name":"Marco Ladetto","orcid":"https://orcid.org/0000-0002-8283-2681","observed_orcids":["https://orcid.org/0000-0002-8283-2681"]},"institutions":[{"id":"https://openalex.org/I55143463","display_name":"University of Turin","ror":"https://ror.org/048tbm396","country_code":"IT","type":"education","lineage":["https://openalex.org/I55143463"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Marco Ladetto","raw_affiliation_strings":["University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Torino, Department of Molecular Biotechnology and Health Sciences, Hematology division at A.O.U. San Giovanni Battista, Torino, Italy,","institution_ids":["https://openalex.org/I55143463"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5006640724","https://openalex.org/A5007558343","https://openalex.org/A5010320947","https://openalex.org/A5012645361","https://openalex.org/A5016042168","https://openalex.org/A5016419122","https://openalex.org/A5036916107","https://openalex.org/A5045333278","https://openalex.org/A5051021130","https://openalex.org/A5053891408","https://openalex.org/A5055456835","https://openalex.org/A5056141793","https://openalex.org/A5079136601","https://openalex.org/A5080278478","https://openalex.org/A5087781254","https://openalex.org/A5103378970","https://openalex.org/A5108524714"],"corresponding_institution_ids":["https://openalex.org/I21449261","https://openalex.org/I4210091033","https://openalex.org/I4210097265","https://openalex.org/I55143463"],"apc_list":{"value":5500,"currency":"USD","value_usd":5500},"apc_paid":null,"fwci":0.4984,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.67117574,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"122","issue":"21","first_page":"4290","last_page":"4290"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.7279999852180481,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.7279999852180481,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10950","display_name":"Acute Lymphoblastic Leukemia research","score":0.12319999933242798,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.05389999970793724,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/measurable-residual-disease","display_name":"minimal residual disease","score":0.9520000219345093},{"id":"https://openalex.org/keywords/immunoglobulin-heavy-chain-gene-rearrangement","display_name":"immunoglobulin heavy chain gene rearrangement","score":0.9110000133514404},{"id":"https://openalex.org/keywords/droplet-digital-pcr","display_name":"droplet digital PCR","score":0.921999990940094},{"id":"https://openalex.org/keywords/real-time-quantitative-pcr","display_name":"real-time quantitative PCR","score":0.9269999861717224},{"id":"https://openalex.org/keywords/mantle-cell-lymphoma","display_name":"mantle cell lymphoma","score":0.9350000023841858},{"id":"https://openalex.org/keywords/multiple-myeloma","display_name":"multiple myeloma","score":1.0},{"id":"https://openalex.org/keywords/absolute-quantification","display_name":"absolute quantification","score":0.796999990940094},{"id":"https://openalex.org/keywords/method-comparison","display_name":"method comparison","score":0.5730000138282776},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.6510000228881836},{"id":"https://openalex.org/keywords/peripheral-blood","display_name":"peripheral blood","score":0.9309999942779541},{"id":"https://openalex.org/keywords/prognostic-assessment","display_name":"prognostic assessment","score":0.5690000057220459},{"id":"https://openalex.org/keywords/sensitivity-and-specificity","display_name":"sensitivity and specificity","score":0.4580000042915344},{"id":"https://openalex.org/keywords/allele-specific-oligonucleotides","display_name":"allele-specific oligonucleotides","score":0.6119999885559082},{"id":"https://openalex.org/keywords/taqman-probes","display_name":"TaqMan probes","score":0.8980000019073486},{"id":"https://openalex.org/keywords/single-molecule-detection","display_name":"single-molecule detection","score":0.33500000834465027}],"concepts":[{"id":"https://openalex.org/C2779823535","wikidata":"https://www.wikidata.org/wiki/Q6865356","display_name":"Minimal residual disease","level":3,"score":0.879274845123291},{"id":"https://openalex.org/C48126324","wikidata":"https://www.wikidata.org/wiki/Q5276136","display_name":"Digital polymerase chain reaction","level":4,"score":0.6946830749511719},{"id":"https://openalex.org/C143660080","wikidata":"https://www.wikidata.org/wiki/Q7684883","display_name":"TaqMan","level":4,"score":0.573498547077179},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.571083664894104},{"id":"https://openalex.org/C184112429","wikidata":"https://www.wikidata.org/wiki/Q476174","display_name":"Serial dilution","level":3,"score":0.5481774806976318},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.5352485775947571},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.45770928263664246},{"id":"https://openalex.org/C2777525834","wikidata":"https://www.wikidata.org/wiki/Q268713","display_name":"Mantle cell lymphoma","level":3,"score":0.4378284215927124},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.42320123314857483},{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.4155377447605133},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.24993467330932617},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22873473167419434},{"id":"https://openalex.org/C2779338263","wikidata":"https://www.wikidata.org/wiki/Q208414","display_name":"Lymphoma","level":2,"score":0.21658816933631897},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.19919264316558838},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.1628696322441101},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13903650641441345},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1182/blood.v122.21.4290.4290","doi":"https://doi.org/10.1182/blood.v122.21.4290.4290","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood.v122.21.4290.4290","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2920830689","https://openalex.org/W2617050295","https://openalex.org/W3016696556","https://openalex.org/W1147404897","https://openalex.org/W2802372704","https://openalex.org/W4285118245","https://openalex.org/W2951661954","https://openalex.org/W4403532843","https://openalex.org/W4252949489","https://openalex.org/W2566576316"],"abstract_inverted_index":{"Abstract":[0],"Background":[1],"In":[2,103,168],"mature":[3],"lymphoid":[4],"disorders,":[5],"minimal":[6],"residual":[7],"disease":[8],"(MRD)":[9],"detection":[10,95,110,181,450],"based":[11,155],"on":[12,74,223,256,432],"real":[13],"time":[14,551],"quantitative":[15,341,354,511],"PCR":[16,82,219,251,264],"(RQ-PCR)":[17],"of":[18,69,87,90,101,124,129,164,226,291,298,309,348,417,419,441,494,554,559,592,602,606,614,621,633],"immunoglobulin":[19],"heavy":[20],"chain":[21],"gene":[22],"rearrangement":[23],"(IgH)":[24],"has":[25,43,49],"a":[26,50,61,97,257,323,340,353,505,568,594,622,641],"well-established":[27],"role":[28],"in":[29,33,53,126,152,161,240,268,415,423,454,461,491,556,563,575],"prognostic":[30],"assessment,":[31],"particularly":[32],"Mantle":[34],"cell":[35],"Lymphoma":[36],"(MCL)":[37],"and":[38,46,92,113,138,150,195,237,273,284,303,336,368,373,384,402,408,430,458,510,577,643],"Multiple":[39],"Myeloma":[40],"(MM).":[41],"RQ-PCR":[42,112,125,154,183,283,304,403,409,518,534,604],"excellent":[44],"sensitivity":[45,412,509],"specificity":[47],"but":[48],"major":[51,329,470],"limitation":[52,131],"its":[54],"relative":[55],"quantification":[56,100,613],"nature,":[57],"as":[58,187,289,321,328,345],"it":[59],"requires":[60],"reference":[62],"standard":[63,595,624],"curve":[64,596],"usually":[65],"built":[66],"with":[67,230,352],"dilutions":[68],"diagnostic":[70,608],"tumor":[71,615],"DNA":[72,91,228],"or":[73,610,617,619],"plasmids":[75],"containing":[76],"the":[77,85,121,127,130,162,173,201,215,231,241,269,331,346,549,552,557,590,600,631],"target":[78,292],"rearrangement.":[79],"Droplet":[80,217],"Digital":[81,218],"(DD-PCR),":[83],"applying":[84],"principle":[86],"limiting":[88],"dilution":[89],"single":[93],"molecule":[94],"allows":[96,588],"reliable":[98],"absolute":[99],"target.":[102],"this":[104],"study":[105],"we":[106,546],"compared":[107,582],"IgH-based":[108,179,560,603,648],"MRD":[109,156,177,180,299,449,561,573,649],"by":[111,148,182,214,246,275,301,307,517,522,533,542],"DD-PCR,":[114],"to":[115,200,363,484,504,583],"assess":[116],"whether":[117],"DD-PCR":[118,211,285,302,401,407,523,555,566,587,627,640],"could":[119],"achieve":[120],"same":[122,232],"performances":[123],"absence":[128],"mentioned":[132],"above.":[133],"Methods":[134],"Bone":[135],"marrow":[136],"(BM)":[137],"peripheral":[139],"blood":[140],"(PB)":[141],"samples":[142,361,379,425,443,457,463,468,475,515,531],"were":[143,197,244,287,319,376,380,389,393,437,444,489,501,519,540],"collected":[144],"from":[145],"patients":[146,171,365],"affected":[147],"MCL":[149,367,372,576],"MM":[151,375],"which":[153],"analysis":[157,314],"was":[158,184,212,254,266,305,326,334,343,398,451],"already":[159],"performed":[160,213,255],"context":[163,558],"prospective":[165],"clinical":[166],"trials.":[167],"all":[169,428],"trials":[170],"gave":[172],"informed":[174],"consent":[175],"for":[176,427,548,571,647],"determination.":[178],"carried":[185],"out":[186,440],"previously":[188],"described":[189],"[Ladetto":[190],"et":[191,207],"al.":[192,208],"BBMT":[193],"2000]":[194],"results":[196,286,300,351],"interpreted":[198],"according":[199],"Euro-MRD":[202],"guidelines":[203],"[van":[204],"der":[205],"Velden":[206],"Leukemia":[209],"2007].":[210],"QX100":[216,247,270],"system":[220],"(Bio-RAD":[221,261],"Inc.)":[222],"500":[224],"ng":[225],"genomic":[227],"combined":[229],"Allele":[233],"Specific":[234],"Oligonucleotides":[235],"(ASO)-primers":[236],"TaqMan-probes":[238],"used":[239],"RQ-PCR.":[242,585],"Droplets":[243],"generated":[245],"droplet":[248,271],"generator.":[249],"End-point":[250],"(40":[252],"cycles)":[253],"T100":[258],"Thermal":[259],"cycler":[260],"Inc).":[262,279],"The":[263],"product":[265],"loaded":[267],"reader":[272],"analyzed":[274],"QuantaSoft":[276],"1.2":[277],"(Bio-Rad":[278],"For":[280],"data":[281],"interpretation":[282],"expressed":[288],"amount":[290],"copies":[293],"per":[294],"1E+05":[295],"cells.":[296],"Comparability":[297],"assessed":[306],"means":[308],"bivariate":[310],"correlations":[311],"between":[312,400,508],"methods":[313],"(R2.15.1":[315],"package":[316],"irr).":[317],"Discordances":[318],"classified":[320],"follows:":[322],"positive/negative":[324],"discordance":[325,342,479],"defined":[327,344],"when":[330,338],"positive":[332,350,453,521,532,634],"result":[333],">1E-04":[335],"minor":[337,477],"\u22641E-04;":[339],"presence":[347],"two":[349],"discrepancy":[355],">1":[356],"log.":[357],"Results":[358],"Overall,":[359],"161":[360,442],"belonging":[362],"35":[364,378],"(18":[366],"17":[369,514],"MM),":[370],"66":[371],"95":[374],"analyzed.":[377],"taken":[381],"at":[382,386],"diagnosis":[383],"126":[385],"follow-up.":[387],"118":[388],"BM":[390],"while":[391,529],"43":[392],"PB.":[394],"A":[395],"significant":[396],"correlation":[397],"found":[399],"(R2=0.89,":[404],"p<0.0001)":[405],"(fig).":[406],"showed":[410,469,476],"superimposable":[411],"(10-5).":[413],"Specificity":[414],"terms":[416],"appearance":[418],"non-specific":[420],"amplifications":[421],"signals":[422],"no-template":[424],"(tested":[426],"patients)":[429],"reproducibility":[431],"30":[433],"replicates":[434],"(4":[435],"samples)":[436],"superimposable.":[438],"128":[439],"fully":[445],"concordant":[446],"(Choen's":[447],"K=0.80).":[448],"concordantly":[452,459],"106/161":[455],"(65.8%)":[456],"negative":[460,516,541],"22/161":[462],"(13.7%).":[464],"Only":[465],"5/161":[466,492],"(3.1%)":[467,493],"qualitative":[471,478],"discordance.":[472],"28/161":[473],"(17.4%)":[474],"(which":[480],"might":[481,628],"be":[482],"related":[483],"Poisson's":[485],"statistics).":[486],"Quantitative":[487],"discordances":[488],"observed":[490],"cases":[495,500],"(positive":[496],"non":[497],"quantifiable":[498],"(PNQ)":[499],"conventionally":[502],"placed":[503],"value":[506],"intermediate":[507],"range).":[512],"Interestingly,":[513],"scored":[520],"(median":[524,535],"6":[525],"copies,":[526,537],"range":[527,538],"2-74)":[528],"16":[530],"5":[536],"2-44)":[539],"DD-PCR.":[543],"Conclusions":[544],"Here":[545],"report":[547],"first":[550],"use":[553],"evaluation":[562],"lymphoproliferative":[564],"disorders.":[565],"is":[567],"feasible":[569,642],"tool":[570],"IGH-based":[572],"monitoring":[574],"MM,":[578],"reaching":[579],"similar":[580],"sensitivities":[581],"standardized":[584],"Moreover":[586],"bypassing":[589],"need":[591],"building":[593,620],"thus":[597],"considerably":[598],"reducing":[599],"complexity":[601],"(need":[605],"purified":[607],"tissue":[609],"Flow":[611],"Cytometry-based":[612],"load":[616],"diagnosis,":[618],"plasmid-derived":[623],"curve).":[625],"Finally":[626],"potentially":[629],"overcome":[630],"problem":[632],"non-quantifiable":[635],"samples.":[636],"These":[637],"features":[638],"make":[639],"attractive":[644],"alternative":[645],"method":[646],"assessment.":[650],"Disclosures:":[651],"Kubiczkov\u00e0:":[652],"GAP304/10/1395":[653],":":[654],"Research":[655,658],"Funding;":[656],"MUNI/11/InGA17/2012:":[657],"Funding.":[659]},"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-09T09:14:12.587421","created_date":"2016-06-24T00:00:00"}