{"id":"https://openalex.org/W135081758","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.912.7","title":"Bioorthogonal Ligation Using Site\u2010Specific Tetrazine\u2010Alkene Coupling On Proteins","display_name":"Bioorthogonal Ligation Using Site\u2010Specific Tetrazine\u2010Alkene Coupling On Proteins","publication_year":2010,"publication_date":"2010-04-01","ids":{"openalex":"https://openalex.org/W135081758","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.912.7","mag":"135081758"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.24.1_supplement.912.7","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.912.7","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.24.1_supplement.912.7","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066781304","display_name":"Christian A. Refakis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I173146385","display_name":"Franklin & Marshall College","ror":"https://ror.org/04fp4ps48","country_code":"US","type":"education","lineage":["https://openalex.org/I173146385"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christian Andr\u00e9 Refakis","raw_affiliation_strings":["Chemistry Franklin and Marshall College Lancaster PA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Franklin and Marshall College Lancaster PA","institution_ids":["https://openalex.org/I173146385"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091047943","display_name":"Melissa L. Blackman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Melissa L Blackman","raw_affiliation_strings":["Chemistry Univeristy of Delaware Newark DE"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Univeristy of Delaware Newark DE","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034235550","display_name":"Joseph M. Fox","orcid":"https://orcid.org/0000-0002-8258-1640","observed_orcids":["https://orcid.org/0000-0002-8258-1640"]},"institutions":[{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph M Fox","raw_affiliation_strings":["Chemistry Univeristy of Delaware Newark DE"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Univeristy of Delaware Newark DE","institution_ids":["https://openalex.org/I86501945"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030497987","display_name":"Ryan A. Mehl","orcid":"https://orcid.org/0000-0003-2932-4941","observed_orcids":["https://orcid.org/0000-0003-2932-4941"]},"institutions":[{"id":"https://openalex.org/I173146385","display_name":"Franklin & Marshall College","ror":"https://ror.org/04fp4ps48","country_code":"US","type":"education","lineage":["https://openalex.org/I173146385"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ryan A Mehl","raw_affiliation_strings":["Chemistry Franklin and Marshall College Lancaster PA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chemistry Franklin and Marshall College Lancaster PA","institution_ids":["https://openalex.org/I173146385"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00762863,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"24","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bioorthogonal-ligation","display_name":"bioorthogonal ligation","score":0.9959999918937683},{"id":"https://openalex.org/keywords/site-specific-protein-labeling","display_name":"site-specific protein labeling","score":0.7319999933242798},{"id":"https://openalex.org/keywords/unnatural-amino-acid-incorporation","display_name":"unnatural amino acid incorporation","score":0.8460000157356262},{"id":"https://openalex.org/keywords/in-vivo-incorporation","display_name":"in vivo incorporation","score":0.4320000112056732},{"id":"https://openalex.org/keywords/drug-conjugation","display_name":"drug conjugation","score":0.5410000085830688}],"concepts":[{"id":"https://openalex.org/C930959","wikidata":"https://www.wikidata.org/wiki/Q4086920","display_name":"Bioorthogonal chemistry","level":3,"score":0.9912756085395813},{"id":"https://openalex.org/C2776254738","wikidata":"https://www.wikidata.org/wiki/Q81406","display_name":"Alkene","level":3,"score":0.8427417278289795},{"id":"https://openalex.org/C2778754187","wikidata":"https://www.wikidata.org/wiki/Q417549","display_name":"Tetrazine","level":2,"score":0.7945054769515991},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7022844552993774},{"id":"https://openalex.org/C99207889","wikidata":"https://www.wikidata.org/wiki/Q5090481","display_name":"Chemical ligation","level":3,"score":0.6746410727500916},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.5952634811401367},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.5759989023208618},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5256796479225159},{"id":"https://openalex.org/C23963754","wikidata":"https://www.wikidata.org/wiki/Q4914729","display_name":"Bioconjugation","level":2,"score":0.5071237087249756},{"id":"https://openalex.org/C2778032875","wikidata":"https://www.wikidata.org/wiki/Q17152469","display_name":"Native chemical ligation","level":4,"score":0.5044354200363159},{"id":"https://openalex.org/C171852809","wikidata":"https://www.wikidata.org/wiki/Q731154","display_name":"Chemical biology","level":2,"score":0.4989290237426758},{"id":"https://openalex.org/C2776910235","wikidata":"https://www.wikidata.org/wiki/Q18574","display_name":"Reactivity (psychology)","level":3,"score":0.46150830388069153},{"id":"https://openalex.org/C2775954774","wikidata":"https://www.wikidata.org/wiki/Q899401","display_name":"Intein","level":5,"score":0.43144428730010986},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.42219099402427673},{"id":"https://openalex.org/C114373084","wikidata":"https://www.wikidata.org/wiki/Q273499","display_name":"Chemical synthesis","level":3,"score":0.27461785078048706},{"id":"https://openalex.org/C69610077","wikidata":"https://www.wikidata.org/wiki/Q912270","display_name":"Click chemistry","level":2,"score":0.24049386382102966},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.2355145812034607},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.19015955924987793},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.08014363050460815},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.0},{"id":"https://openalex.org/C204787440","wikidata":"https://www.wikidata.org/wiki/Q188504","display_name":"Alternative medicine","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.24.1_supplement.912.7","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.912.7","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.24.1_supplement.912.7","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2034097144","https://openalex.org/W2792698539","https://openalex.org/W4411989600","https://openalex.org/W2792192319","https://openalex.org/W4382989488","https://openalex.org/W946883613","https://openalex.org/W7172105059","https://openalex.org/W1984981795","https://openalex.org/W2036809730","https://openalex.org/W1968248603"],"abstract_inverted_index":{"The":[0,61,89,110],"selective":[1],"coupling":[2],"of":[3,63,72,112,116,139],"chemical":[4,152],"labels":[5,153],"to":[6,10,24,35,57,67,84,156],"proteins":[7,99],"is":[8,66],"key":[9],"many":[11],"applications":[12],"in":[13,101],"medicine":[14],"and":[15,27,54,96,133],"biotechnology.":[16],"Chemical":[17],"labeling":[18],"strategies":[19],"require":[20],"two":[21],"functional":[22],"groups":[23],"react":[25,55],"rapidly":[26],"selectively":[28],"with":[29,120,129,145],"one":[30],"another":[31],"under":[32],"biological":[33],"conditions":[34],"form":[36],"a":[37,113],"stable":[38],"covalent":[39],"bond.":[40],"Despite":[41],"their":[42],"great":[43],"utility,":[44],"few":[45],"effective":[46],"bioorthogonal":[47,74],"ligation":[48,75,126],"reactions":[49,76],"exist":[50],"that":[51],"are":[52],"non\u2010toxic":[53],"specifically":[56],"the":[58,70,86,124,137],"programmed":[59],"functionality.":[60],"objective":[62],"this":[64,140],"study":[65],"expand":[68],"on":[69],"array":[71],"available":[73],"by":[77],"using":[78,103],"site\u2010specific":[79],"unnatural":[80,105],"amino":[81,90],"acid":[82,91],"incorporation":[83],"exploit":[85],"alkene\u2010tetrazine":[87],"reaction.":[88],"p\u2010vinyl\u2010L\u2010phenylalanine":[92],"(pVF)":[93],"was":[94,118],"synthesized":[95],"incorporated":[97],"into":[98],"site\u2010specifically,":[100],"vivo,":[102],"an":[104,146],"aminoacyl\u2010tRNA":[106],"synthetase":[107],"(RS)":[108],"pair.":[109],"reactivity":[111],"diverse":[114],"family":[115],"tetrazines":[117],"explored":[119],"pVF\u2010protein.":[121],"ESI\u2010MS":[122],"confirmed":[123],"tetrzine\u2010alkene":[125],"reaction":[127],"occurs":[128],"high":[130],"fidelity,":[131],"site\u2010specificity,":[132],"yield.":[134],"We":[135],"expect":[136],"results":[138],"work":[141],"will":[142],"provide":[143],"scientists":[144],"additional":[147],"venue":[148],"for":[149],"attaching":[150],"small":[151],"and/or":[154],"drugs":[155],"proteins.":[157]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}
