{"id":"https://openalex.org/W132814258","doi":null,"title":"A Novel Assay for the Quantification of Active Transcription Factors","display_name":"A Novel Assay for the Quantification of Active Transcription Factors","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W132814258","mag":"132814258"},"language":"en","primary_location":{"id":"pmh:oai:uilis.unsyiah.ac.id:32810","doi":null,"is_oa":true,"landing_page_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/32810","pdf_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/32810","source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/32810","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5008232995","display_name":"Isabel M. Goodhand","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Isabel M. Goodhand","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5008232995"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.6104000210762024,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.6104000210762024,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.1282999962568283,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.10360000282526016,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transcription-factors","display_name":"transcription factors","score":0.8209999799728394},{"id":"https://openalex.org/keywords/ps3","display_name":"PS3","score":0.6539999842643738},{"id":"https://openalex.org/keywords/synthetic-oligonucleotide-probes","display_name":"synthetic oligonucleotide probes","score":0.7450000047683716},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7609999775886536},{"id":"https://openalex.org/keywords/dna-binding-activity","display_name":"DNA-binding activity","score":0.8050000071525574},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.7639999985694885},{"id":"https://openalex.org/keywords/cancer","display_name":"cancer","score":0.7860000133514404}],"concepts":[{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.7791456580162048},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.5819505453109741},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5643181800842285},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.5537720918655396},{"id":"https://openalex.org/C144825837","wikidata":"https://www.wikidata.org/wiki/Q2316030","display_name":"General transcription factor","level":5,"score":0.547176718711853},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.48213526606559753},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.39924126863479614},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3518626391887665},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34274595975875854},{"id":"https://openalex.org/C101762097","wikidata":"https://www.wikidata.org/wiki/Q224093","display_name":"Promoter","level":4,"score":0.2236935794353485},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2101781964302063},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13714191317558289},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.12384837865829468},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:uilis.unsyiah.ac.id:32810","doi":null,"is_oa":true,"landing_page_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/32810","pdf_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/32810","source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:hdl:10068/1010655","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10068/1010655","pdf_url":null,"source":{"id":"https://openalex.org/S4377196900","display_name":"OpenGrey (Institut de l'Information Scientifique et Technique)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1295345811","host_organization_name":"Institut de l'Information Scientifique et Technique","host_organization_lineage":["https://openalex.org/I1295345811"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"U - Thesis"},{"id":"mag:132814258","doi":null,"is_oa":false,"landing_page_url":"https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603315","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:uilis.unsyiah.ac.id:32810","doi":null,"is_oa":true,"landing_page_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/32810","pdf_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/32810","source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W132814258.pdf","grobid_xml":"https://content.openalex.org/works/W132814258.grobid-xml"},"referenced_works_count":155,"referenced_works":["https://openalex.org/W168933712","https://openalex.org/W421135038","https://openalex.org/W1502887285","https://openalex.org/W1505173609","https://openalex.org/W1519057123","https://openalex.org/W1531836615","https://openalex.org/W1588914893","https://openalex.org/W1608920950","https://openalex.org/W1727290854","https://openalex.org/W1760501277","https://openalex.org/W1799809595","https://openalex.org/W1903324674","https://openalex.org/W1964821219","https://openalex.org/W1965267927","https://openalex.org/W1968682237","https://openalex.org/W1971224531","https://openalex.org/W1976209715","https://openalex.org/W1976991089","https://openalex.org/W1977686001","https://openalex.org/W1978782811","https://openalex.org/W1982259691","https://openalex.org/W1982840146","https://openalex.org/W1983171715","https://openalex.org/W1985544563","https://openalex.org/W1986179131","https://openalex.org/W1988214676","https://openalex.org/W1989080074","https://openalex.org/W1993009001","https://openalex.org/W1993831923","https://openalex.org/W1994017669","https://openalex.org/W1995095999","https://openalex.org/W1995884552","https://openalex.org/W1996649572","https://openalex.org/W1998593414","https://openalex.org/W1998876947","https://openalex.org/W2001962665","https://openalex.org/W2003058837","https://openalex.org/W2003236156","https://openalex.org/W2003249240","https://openalex.org/W2004273407","https://openalex.org/W2004563262","https://openalex.org/W2006174096","https://openalex.org/W2006975469","https://openalex.org/W2009071546","https://openalex.org/W2010397777","https://openalex.org/W2011465285","https://openalex.org/W2012761949","https://openalex.org/W2015314126","https://openalex.org/W2016086822","https://openalex.org/W2018385269","https://openalex.org/W2020365958","https://openalex.org/W2020515626","https://openalex.org/W2020997064","https://openalex.org/W2022395375","https://openalex.org/W2022762967","https://openalex.org/W2023886879","https://openalex.org/W2029215616","https://openalex.org/W2029898130","https://openalex.org/W2032462421","https://openalex.org/W2033184158","https://openalex.org/W2033989981","https://openalex.org/W2034269086","https://openalex.org/W2039760853","https://openalex.org/W2043364653","https://openalex.org/W2046427987","https://openalex.org/W2046632178","https://openalex.org/W2046958356","https://openalex.org/W2049499385","https://openalex.org/W2049527615","https://openalex.org/W2051182140","https://openalex.org/W2052853635","https://openalex.org/W2053021213","https://openalex.org/W2055280366","https://openalex.org/W2058399266","https://openalex.org/W2058769115","https://openalex.org/W2063669247","https://openalex.org/W2065914817","https://openalex.org/W2066547576","https://openalex.org/W2066706759","https://openalex.org/W2067419418","https://openalex.org/W2068351024","https://openalex.org/W2069645361","https://openalex.org/W2072319696","https://openalex.org/W2074114480","https://openalex.org/W2074222004","https://openalex.org/W2074370114","https://openalex.org/W2075300416","https://openalex.org/W2078672321","https://openalex.org/W2080684604","https://openalex.org/W2084855813","https://openalex.org/W2086260870","https://openalex.org/W2086802511","https://openalex.org/W2087401860","https://openalex.org/W2087562553","https://openalex.org/W2088584454","https://openalex.org/W2089062027","https://openalex.org/W2089281483","https://openalex.org/W2091942447","https://openalex.org/W2092902276","https://openalex.org/W2093772131","https://openalex.org/W2095642182","https://openalex.org/W2095732342","https://openalex.org/W2096199069","https://openalex.org/W2098326902","https://openalex.org/W2099907467","https://openalex.org/W2101136061","https://openalex.org/W2101155068","https://openalex.org/W2104802032","https://openalex.org/W2107550595","https://openalex.org/W2107739085","https://openalex.org/W2110569721","https://openalex.org/W2111817213","https://openalex.org/W2117521650","https://openalex.org/W2125600615","https://openalex.org/W2128985829","https://openalex.org/W2129795133","https://openalex.org/W2133538902","https://openalex.org/W2133959309","https://openalex.org/W2136261892","https://openalex.org/W2137124052","https://openalex.org/W2141393790","https://openalex.org/W2142495011","https://openalex.org/W2143778153","https://openalex.org/W2146690493","https://openalex.org/W2147620559","https://openalex.org/W2150114991","https://openalex.org/W2150965482","https://openalex.org/W2151160119","https://openalex.org/W2154027407","https://openalex.org/W2154799239","https://openalex.org/W2154976095","https://openalex.org/W2156273110","https://openalex.org/W2156299289","https://openalex.org/W2158734599","https://openalex.org/W2160064408","https://openalex.org/W2160450758","https://openalex.org/W2162191065","https://openalex.org/W2163565167","https://openalex.org/W2164436574","https://openalex.org/W2167155713","https://openalex.org/W2168087339","https://openalex.org/W2168106779","https://openalex.org/W2170494099","https://openalex.org/W2170597941","https://openalex.org/W2170775366","https://openalex.org/W2170949754","https://openalex.org/W2218138196","https://openalex.org/W2266039028","https://openalex.org/W2326503737","https://openalex.org/W2332303086","https://openalex.org/W2411854994","https://openalex.org/W2419345597","https://openalex.org/W2501003502","https://openalex.org/W2530014580","https://openalex.org/W2774846296"],"related_works":["https://openalex.org/W2052541844","https://openalex.org/W4241224272","https://openalex.org/W2321092928","https://openalex.org/W2093498207","https://openalex.org/W2023548434","https://openalex.org/W2282018349","https://openalex.org/W1993339363","https://openalex.org/W2746052022","https://openalex.org/W2805457033","https://openalex.org/W2466974034"],"abstract_inverted_index":{"Transcription":[0],"factors":[1,40,73],"are":[2,276],"a":[3,44,83,87,96,127,133,156,162,175,222,250],"family":[4],"of":[5,12,19,26,30,38,93,121,138,174,184,205,225,252,259,268],"DNA-binding":[6,165],"proteins":[7],"involved":[8,33],"in":[9,34,256,282],"the":[10,24,28,136,143,151,172,181,215,229,257],"regulation":[11],"all":[13],"cellular":[14],"processes.":[15],"One":[16],"particular":[17],"area":[18],"transcription":[20,39,61,72,140,148,163,182,208],"factor":[21,62,183,209],"function":[22],"is":[23,32,169,195],"process":[25],"apoptosis,":[27],"deregulation":[29],"which":[31],"cancer.":[35],"The":[36,119,192,203],"study":[37],"may":[41],"therefore":[42],"provide":[43],"novel":[45],"perspective":[46],"on":[47,171],"cancer":[48],"aetiology,":[49],"as":[50,52,155],"well":[51],"insight":[53],"into":[54,60],"potential":[55],"treatment":[56],"opportunities.":[57],"However,":[58,255],"research":[59],"activity":[63],"has":[64],"traditionally":[65],"been":[66],"hindered":[67],"by":[68,214,234],"technical":[69],"limitations.":[70],"Firstly,":[71],"being":[74],"such":[75],"potent":[76],"molecules":[77,160],"need":[78],"only":[79],"be":[80],"present":[81,107],"at":[82],"low":[84],"concentration":[85],"within":[86],"cell,":[88],"thus":[89],"requiring":[90],"sensitive":[91],"methods":[92],"detection,":[94],"or":[95],"large":[97],"cell":[98],"sample":[99],"for":[100,109,135,145,158,266],"analysis.":[101],"Secondly,":[102],"their":[103,116],"tertiary":[104],"protein":[105],"structures":[106],"problems":[108],"interactions":[110],"with":[111,142,180,249,286],"inhibitory":[112],"pharmaceutical":[113],"agents,":[114],"limiting":[115],"clinical":[117],"application.":[118],"aim":[120],"this":[122,241,260,280],"project":[123],"was":[124,212,243,263],"to":[125,129,219,245,278,284],"develop":[126],"method":[128,134,168,242],"overcome":[130],"these":[131],"obstacles;":[132],"quantification":[137],"active":[139],"factors,":[141,149],"sensitivity":[144,251],"analysing":[146],"endogenous":[147,269],"and":[150,210,228],"high":[152],"throughput":[153],"adaptability":[154],"screen":[157],"testing":[159],"altering":[161],"factor's":[164],"activity.":[166],"This":[167],"based":[170,293],"isolation":[173],"synthetic":[176,193,206,230],"oligonucleotide":[177,194,231],"probe":[178],"complexed":[179],"interest,":[185],"using":[186,198],"Protein-A":[187],"conjugated":[188],"magnetic":[189],"bead":[190],"immune-purification.":[191],"subsequently":[196],"quantified":[197],"quantitative":[199,235],"Polymerase":[200,236],"Chain":[201,237],"Reaction.":[202,238],"complex":[204],"oligonucleotide,":[207],"antibody":[211],"shown":[213],"Electromobility":[216],"supershift":[217],"assay":[218],"form":[220],"under":[221],"defined":[223],"set":[224],"experimental":[226],"conditions,":[227],"reliably":[232],"detected":[233],"Following":[239],"optimisation,":[240],"able":[244],"quantify":[246],"purified":[247],"pS3,":[248],"S":[253],"ng,":[254],"scope":[258],"project,":[261],"performance":[262],"not":[264],"sufficient":[265],"detection":[267],"pS3":[270],"from":[271],"LS174T":[272],"cells.":[273],"Further":[274],"optimisations":[275],"required":[277],"reach":[279],"goal":[281],"order":[283],"compete":[285],"commercially":[287],"available":[288],"Enzyme":[289],"Linked":[290],"Immunosorbent":[291],"Assay":[292],"methods.":[294]},"counts_by_year":[],"updated_date":"2026-08-31T07:19:18.363097","created_date":"2016-06-24T00:00:00"}