{"id":"https://openalex.org/W131802134","doi":null,"title":"Investigation of the process of SNARE mediated synaptic vesicle exocytosis","display_name":"Investigation of the process of SNARE mediated synaptic vesicle exocytosis","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W131802134","mag":"131802134"},"language":"en","primary_location":{"id":"pmh:oai:lib.dr.iastate.edu:etd-4238","doi":null,"is_oa":false,"landing_page_url":"https://dr.lib.iastate.edu/bitstreams/1159d9ef-d743-453d-835f-c2b9b0824c7a/download","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043355901","display_name":"Ying Lai","orcid":"https://orcid.org/0000-0001-5347-5155","observed_orcids":["https://orcid.org/0000-0001-5347-5155"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ying Lai","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-5347-5155","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5043355901"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00782071,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/snare-complex","display_name":"SNARE complex","score":0.796999990940094},{"id":"https://openalex.org/keywords/synaptic-vesicle-exocytosis","display_name":"synaptic vesicle exocytosis","score":0.9950000047683716},{"id":"https://openalex.org/keywords/neurotransmitter-release","display_name":"neurotransmitter release","score":0.8970000147819519},{"id":"https://openalex.org/keywords/synaptotagmin-1","display_name":"synaptotagmin-1","score":0.9279999732971191},{"id":"https://openalex.org/keywords/calcium-indicator","display_name":"calcium sensor","score":0.49300000071525574},{"id":"https://openalex.org/keywords/vesicle-docking","display_name":"vesicle docking","score":0.656000018119812},{"id":"https://openalex.org/keywords/alpha-synuclein","display_name":"alpha-synuclein","score":0.7059999704360962},{"id":"https://openalex.org/keywords/proteoliposomes","display_name":"proteoliposomes","score":0.777999997138977},{"id":"https://openalex.org/keywords/neurodegeneration","display_name":"neurodegeneration","score":0.5289999842643738}],"concepts":[{"id":"https://openalex.org/C175969161","wikidata":"https://www.wikidata.org/wiki/Q323426","display_name":"Exocytosis","level":3,"score":0.7734742760658264},{"id":"https://openalex.org/C65972015","wikidata":"https://www.wikidata.org/wiki/Q6935634","display_name":"Munc-18","level":5,"score":0.5784654021263123},{"id":"https://openalex.org/C165254790","wikidata":"https://www.wikidata.org/wiki/Q6416872","display_name":"Kiss-and-run fusion","level":5,"score":0.541355311870575},{"id":"https://openalex.org/C98045186","wikidata":"https://www.wikidata.org/wiki/Q205663","display_name":"Process (computing)","level":2,"score":0.5409896969795227},{"id":"https://openalex.org/C148785051","wikidata":"https://www.wikidata.org/wiki/Q671136","display_name":"Synaptic vesicle","level":4,"score":0.48305708169937134},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.46185314655303955},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43967029452323914},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.4051324427127838},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3259583115577698},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.256093829870224},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23655155301094055},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.16335561871528625},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.11143282055854797},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.09319418668746948},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:lib.dr.iastate.edu:etd-4238","doi":null,"is_oa":false,"landing_page_url":"https://dr.lib.iastate.edu/bitstreams/1159d9ef-d743-453d-835f-c2b9b0824c7a/download","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},{"id":"mag:131802134","doi":null,"is_oa":false,"landing_page_url":"https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=4238&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":153,"referenced_works":["https://openalex.org/W115728988","https://openalex.org/W1520187505","https://openalex.org/W1521854746","https://openalex.org/W1527671059","https://openalex.org/W1529696685","https://openalex.org/W1530469978","https://openalex.org/W1531179421","https://openalex.org/W1591133722","https://openalex.org/W1796620477","https://openalex.org/W1822252127","https://openalex.org/W1869532508","https://openalex.org/W1963551844","https://openalex.org/W1965912535","https://openalex.org/W1968563729","https://openalex.org/W1970237920","https://openalex.org/W1971116939","https://openalex.org/W1971509671","https://openalex.org/W1973850881","https://openalex.org/W1974537340","https://openalex.org/W1975194767","https://openalex.org/W1976479871","https://openalex.org/W1979944790","https://openalex.org/W1980955669","https://openalex.org/W1982379525","https://openalex.org/W1985321331","https://openalex.org/W1987600267","https://openalex.org/W1990220309","https://openalex.org/W1990473626","https://openalex.org/W1990942267","https://openalex.org/W1997239951","https://openalex.org/W1998948607","https://openalex.org/W1999213641","https://openalex.org/W1999367496","https://openalex.org/W1999702498","https://openalex.org/W1999814206","https://openalex.org/W2000003653","https://openalex.org/W2000302106","https://openalex.org/W2003977518","https://openalex.org/W2006766519","https://openalex.org/W2007521423","https://openalex.org/W2009417622","https://openalex.org/W2009573330","https://openalex.org/W2009639277","https://openalex.org/W2011940688","https://openalex.org/W2012830151","https://openalex.org/W2017040646","https://openalex.org/W2019897261","https://openalex.org/W2020593055","https://openalex.org/W2021285837","https://openalex.org/W2022294768","https://openalex.org/W2022909779","https://openalex.org/W2023863818","https://openalex.org/W2024136850","https://openalex.org/W2024807915","https://openalex.org/W2027775552","https://openalex.org/W2029160544","https://openalex.org/W2029667189","https://openalex.org/W2029861628","https://openalex.org/W2030183236","https://openalex.org/W2030564316","https://openalex.org/W2030873392","https://openalex.org/W2031051437","https://openalex.org/W2035849717","https://openalex.org/W2036664829","https://openalex.org/W2037702696","https://openalex.org/W2039794026","https://openalex.org/W2040025072","https://openalex.org/W2042089363","https://openalex.org/W2047426396","https://openalex.org/W2047683495","https://openalex.org/W2047705116","https://openalex.org/W2050333027","https://openalex.org/W2050367367","https://openalex.org/W2051393046","https://openalex.org/W2052975546","https://openalex.org/W2053515408","https://openalex.org/W2056293200","https://openalex.org/W2056352332","https://openalex.org/W2058035029","https://openalex.org/W2063253418","https://openalex.org/W2066594212","https://openalex.org/W2068323391","https://openalex.org/W2068602144","https://openalex.org/W2068712507","https://openalex.org/W2069491212","https://openalex.org/W2072023677","https://openalex.org/W2073850595","https://openalex.org/W2074180389","https://openalex.org/W2074263866","https://openalex.org/W2075402444","https://openalex.org/W2077451533","https://openalex.org/W2078320842","https://openalex.org/W2079405491","https://openalex.org/W2083922554","https://openalex.org/W2085680244","https://openalex.org/W2085871244","https://openalex.org/W2086696756","https://openalex.org/W2089333920","https://openalex.org/W2091298684","https://openalex.org/W2091653401","https://openalex.org/W2094337062","https://openalex.org/W2098744542","https://openalex.org/W2104270687","https://openalex.org/W2106723144","https://openalex.org/W2113632335","https://openalex.org/W2114795136","https://openalex.org/W2116535065","https://openalex.org/W2118639186","https://openalex.org/W2118978641","https://openalex.org/W2121766064","https://openalex.org/W2121966104","https://openalex.org/W2123151328","https://openalex.org/W2123184492","https://openalex.org/W2123355036","https://openalex.org/W2127061791","https://openalex.org/W2128292852","https://openalex.org/W2129430733","https://openalex.org/W2130323165","https://openalex.org/W2132213868","https://openalex.org/W2138936877","https://openalex.org/W2140225001","https://openalex.org/W2140791749","https://openalex.org/W2142311913","https://openalex.org/W2142844453","https://openalex.org/W2144447780","https://openalex.org/W2144944458","https://openalex.org/W2146841137","https://openalex.org/W2150656456","https://openalex.org/W2150670276","https://openalex.org/W2150981663","https://openalex.org/W2151858671","https://openalex.org/W2153536347","https://openalex.org/W2154100802","https://openalex.org/W2154717765","https://openalex.org/W2156465149","https://openalex.org/W2158930854","https://openalex.org/W2160572200","https://openalex.org/W2160818993","https://openalex.org/W2161334375","https://openalex.org/W2161367731","https://openalex.org/W2161835786","https://openalex.org/W2164220708","https://openalex.org/W2167912328","https://openalex.org/W2168979413","https://openalex.org/W2170250764","https://openalex.org/W2318883842","https://openalex.org/W2327760322","https://openalex.org/W2805648322","https://openalex.org/W2808176952","https://openalex.org/W2808291996","https://openalex.org/W2894613750","https://openalex.org/W2900492910","https://openalex.org/W3135190686"],"related_works":["https://openalex.org/W2338997339","https://openalex.org/W11495774","https://openalex.org/W2913907759","https://openalex.org/W3005199604","https://openalex.org/W2941374900","https://openalex.org/W2054872696","https://openalex.org/W2172212230","https://openalex.org/W2968364592","https://openalex.org/W2166747217","https://openalex.org/W2517016712"],"abstract_inverted_index":{"Neurotransmitter":[0],"release":[1,20],"in":[2,36,42,259,325,341],"synapses":[3],"underlies":[4],"major":[5,243],"brain":[6,25],"functions":[7],"such":[8,59],"as":[9,241],"cognition,":[10],"emotion,":[11],"and":[12,56,69,115,130,141,172,207,253,363],"memory.":[13],"The":[14,90],"precise":[15],"temporal":[16,102],"control":[17],"of":[18,45,86,127,136,166,286],"the":[19,32,43,84,96,125,134,164,181,213,268,277,282,287,318,327,342,352,358,367],"is":[21,48,219,251,323],"essential":[22],"for":[23,237,246,360,365],"healthy":[24],"activities.":[26],"SNAREs":[27],"are":[28,149,349],"known":[29],"to":[30,100,162,175,222,239,262,267,276,280,296,306],"be":[31],"core":[33],"fusion":[34,224,288,319,328,368],"machinery":[35,129],"neuro-exocytotic":[37],"pathways.":[38],"SNARE":[39,91,203,210,313],"complex":[40],"formation":[41,165],"chasm":[44],"two":[46,65,87],"membranes":[47],"mediated":[49],"by":[50,230],"cognate":[51],"coiled-coil":[52],"motifs":[53],"on":[54],"v-":[55],"t-SNAREs:":[57],"One":[58],"motif":[60],"from":[61,66,71],"t-SNARE":[62,67],"syntaxin":[63],"1A,":[64],"SNAP-25,":[68],"another":[70],"v-SNARE":[72],"VAMP2":[73],"all":[74],"form":[75],"a":[76,119,242,257],"parallel":[77],"four-stranded":[78],"coiled":[79],"coil":[80],"that":[81,106,190,236,312],"brings":[82],"about":[83],"apposition":[85],"membranes.":[88],"However,":[89,133],"proteins":[92,110,144],"do":[93],"not":[94],"have":[95],"required":[97],"regulatory":[98,109],"function":[99,240],"confer":[101],"on/off":[103],"switching":[104],"capability":[105],"requires":[107],"other":[108],"like":[111],"Munc13/Munc18,":[112],"complexin,":[113],"synaptotagmin,":[114],"so":[116],"on.":[117],"Moreover,":[118,205],"relationship":[120],"has":[121],"been":[122],"discovered":[123],"between":[124,169],"malfunction":[126],"neurotransmitter-release":[128],"neuro-degeneration":[131,142],"diseases.":[132],"mechanisms":[135],"just":[137],"how":[138],"these":[139],"regulators":[140],"related":[143],"mediate":[145],"synaptic":[146],"vesicle":[147,195,361],"exocytosis":[148],"still":[150],"unknown.\\nIn":[151],"our":[152,337],"work,":[153],"we":[154,187,234,290,310],"developed":[155,291],"site-specific":[156],"fluorescence":[157],"resonance":[158],"energy":[159],"transfer":[160],"(FRET)":[161],"investigate":[163],"trans":[167,202,265],"SNAREpin":[168],"complimentary":[170],"t-":[171],"v-SNAREs":[173],"reconstituted":[174],"separate":[176],"proteoliposomes.":[177],"In":[178],"combination":[179],"with":[180,301,331,356],"traditional":[182],"bulk":[183],"lipid":[184],"mixing":[185,295,305],"assay,":[186],"first":[188],"found":[189],"\u03b1-synulcein":[191],"may":[192,255],"inhibit":[193],"SNARE-dependent":[194],"docking":[196,362],"through":[197],"membrane":[198,214,270],"binding":[199,266,275],"without":[200],"affecting":[201],"assembly.":[204],"C2AB":[206,218],"Ca2+":[208,244],"drive":[209],"zippering":[211],"at":[212],"proximal":[215],"region,":[216,345],"although":[217],"less":[220],"sufficient":[221],"stimulation":[223],"pore":[225,298,308,329,334],"opening":[226,284,326,366],"than":[227,272],"Syt1.":[228],"Third,":[229],"studying":[231],"full-length":[232],"Syt1,":[233],"demonstrated":[235],"Syt1":[238,261,343,353],"sensor":[245],"neuro-exocytosis,":[247],"vectorial":[248],"surface-charge":[249],"asymmetry":[250],"required,":[252],"this":[254],"play":[256],"role":[258],"steering":[260],"achieve":[263],"productive":[264],"plasma":[269],"rather":[271],"non-productive":[273],"cis":[274],"vesicle.":[278],"Fourth,":[279],"study":[281],"fusion-pore":[283],"step":[285],"process,":[289],"large":[292],"DNA-probe-based":[293],"content":[294,304],"report":[297],"expansion.":[299,335],"Together":[300],"small-probe":[302],"sulforhodamine-based":[303],"indicate":[307],"opening,":[309],"showed":[311],"alone":[314],"can":[315],"inefficiently":[316],"open":[317],"pore,":[320],"while":[321],"Syt1/Ca2+":[322],"efficient":[324],"but":[330],"relatively":[332],"slow":[333],"Finally,":[336],"results":[338],"suggest":[339],"that,":[340],"linker":[344,354],"charged":[346],"amino":[347],"acids":[348],"asymmetrically-located,":[350],"providing":[351],"region":[355],"both":[357],"flexibility":[359],"rigidity":[364],"pore.":[369]},"counts_by_year":[],"updated_date":"2026-09-11T09:22:31.653822","created_date":"2016-06-24T00:00:00"}
