{"id":"https://openalex.org/W1225833829","doi":"https://doi.org/10.1021/acs.biochem.5b00689","title":"Taq DNA Polymerase Mutants and 2\u2032-Modified Sugar Recognition","display_name":"Taq DNA Polymerase Mutants and 2\u2032-Modified Sugar Recognition","publication_year":2015,"publication_date":"2015-09-03","ids":{"openalex":"https://openalex.org/W1225833829","doi":"https://doi.org/10.1021/acs.biochem.5b00689","mag":"1225833829","pmid":"https://pubmed.ncbi.nlm.nih.gov/26334839"},"language":"en","primary_location":{"id":"doi:10.1021/acs.biochem.5b00689","doi":"https://doi.org/10.1021/acs.biochem.5b00689","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.biochem.5b00689","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025021732","display_name":"Hayley J. Schultz","orcid":"https://orcid.org/0000-0001-5787-2772","observed_orcids":["https://orcid.org/0000-0001-5787-2772"]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hayley J. Schultz","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070452519","display_name":"Andrea Gochi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrea M. Gochi","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091081024","display_name":"Hannah E. Chia","orcid":"https://orcid.org/0000-0002-1132-329X","observed_orcids":["https://orcid.org/0000-0002-1132-329X"]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hannah E. Chia","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072255154","display_name":"Alexie L. Ogonowsky","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexie L. Ogonowsky","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Sharon Chiang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sharon Chiang","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068668831","display_name":"Nedim Filipovic","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nedim Filipovic","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025183944","display_name":"Aurora G. Weiden","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aurora G. Weiden","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069982870","display_name":"Emma Hadley","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Emma E. Hadley","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068703278","display_name":"Sara E. Gabriel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sara E. Gabriel","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047874372","display_name":"Aaron M. Leconte","orcid":"https://orcid.org/0000-0002-5789-9147","observed_orcids":["https://orcid.org/0000-0002-5789-9147"]},"institutions":[{"id":"https://openalex.org/I106107269","display_name":"Claremont McKenna College","ror":"https://ror.org/04n1me355","country_code":"US","type":"education","lineage":["https://openalex.org/I106107269"]},{"id":"https://openalex.org/I141778976","display_name":"Pitzer College","ror":"https://ror.org/0197n2v40","country_code":"US","type":"education","lineage":["https://openalex.org/I141778976"]},{"id":"https://openalex.org/I16970960","display_name":"Scripps College","ror":"https://ror.org/00p55jd14","country_code":"US","type":"education","lineage":["https://openalex.org/I16970960"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Aaron M. Leconte","raw_affiliation_strings":["W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"W. M. Keck Science Department, Claremont McKenna, Pitzer, and Scripps Colleges , Claremont, California 91711, United States","institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5047874372"],"corresponding_institution_ids":["https://openalex.org/I106107269","https://openalex.org/I141778976","https://openalex.org/I16970960"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.0121,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.73775025,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"54","issue":"38","first_page":"5999","last_page":"6008"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.7461000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.7461000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.17679999768733978,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.01590000092983246,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/taq-polymerase","display_name":"Taq DNA polymerase","score":0.9269999861717224},{"id":"https://openalex.org/keywords/enzyme-mutants","display_name":"enzyme mutants","score":0.4390000104904175},{"id":"https://openalex.org/keywords/substrate-recognition","display_name":"substrate recognition","score":0.7720000147819519},{"id":"https://openalex.org/keywords/directed-evolution","display_name":"directed evolution","score":0.609000027179718},{"id":"https://openalex.org/keywords/rational-enzyme-design","display_name":"rational enzyme design","score":0.6919999718666077}],"concepts":[{"id":"https://openalex.org/C2781440490","wikidata":"https://www.wikidata.org/wiki/Q137165","display_name":"Thermus aquaticus","level":4,"score":0.788772463798523},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6693280935287476},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.6353923082351685},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.5801193118095398},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.5660508871078491},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5635578632354736},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.552102267742157},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.5454748868942261},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5095041394233704},{"id":"https://openalex.org/C2779186596","wikidata":"https://www.wikidata.org/wiki/Q412581","display_name":"Taq polymerase","level":5,"score":0.5091835856437683},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5050740838050842},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.45105046033859253},{"id":"https://openalex.org/C2776027139","wikidata":"https://www.wikidata.org/wiki/Q7783265","display_name":"Thermus","level":4,"score":0.4360354244709015},{"id":"https://openalex.org/C31254050","wikidata":"https://www.wikidata.org/wiki/Q22328799","display_name":"DNA clamp","level":5,"score":0.4261445701122284},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.42571109533309937},{"id":"https://openalex.org/C164965036","wikidata":"https://www.wikidata.org/wiki/Q2529148","display_name":"DNA polymerase I","level":5,"score":0.41207677125930786},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3506867289543152},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.28879764676094055},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.21698635816574097},{"id":"https://openalex.org/C27911776","wikidata":"https://www.wikidata.org/wiki/Q834023","display_name":"Thermophile","level":3,"score":0.0},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D009711","descriptor_name":"Nucleotides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009711","descriptor_name":"Nucleotides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013824","descriptor_name":"Thermus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013824","descriptor_name":"Thermus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013824","descriptor_name":"Thermus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013824","descriptor_name":"Thermus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017354","descriptor_name":"Point Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019914","descriptor_name":"Taq Polymerase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D019914","descriptor_name":"Taq Polymerase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019914","descriptor_name":"Taq Polymerase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/acs.biochem.5b00689","doi":"https://doi.org/10.1021/acs.biochem.5b00689","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.biochem.5b00689","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmid:26334839","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26334839","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3665742663","display_name":null,"funder_award_id":"22548","funder_id":"https://openalex.org/F4320306487","funder_display_name":"Research Corporation for Science Advancement"}],"funders":[{"id":"https://openalex.org/F4320306487","display_name":"Research Corporation for Science Advancement","ror":"https://ror.org/00jp8d455"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":44,"referenced_works":["https://openalex.org/W1551009163","https://openalex.org/W1965194080","https://openalex.org/W1967902778","https://openalex.org/W1971377724","https://openalex.org/W1977668555","https://openalex.org/W1986534666","https://openalex.org/W1988216112","https://openalex.org/W1994092462","https://openalex.org/W1998588829","https://openalex.org/W2008819279","https://openalex.org/W2011377047","https://openalex.org/W2011562612","https://openalex.org/W2012797635","https://openalex.org/W2022688526","https://openalex.org/W2032118018","https://openalex.org/W2050247825","https://openalex.org/W2050387685","https://openalex.org/W2052484845","https://openalex.org/W2052781717","https://openalex.org/W2055528600","https://openalex.org/W2061149630","https://openalex.org/W2061465687","https://openalex.org/W2061680337","https://openalex.org/W2063606548","https://openalex.org/W2068659296","https://openalex.org/W2074713448","https://openalex.org/W2081531630","https://openalex.org/W2083616122","https://openalex.org/W2088880971","https://openalex.org/W2089891949","https://openalex.org/W2091984642","https://openalex.org/W2095186892","https://openalex.org/W2101539429","https://openalex.org/W2110416817","https://openalex.org/W2111066814","https://openalex.org/W2127914992","https://openalex.org/W2127960534","https://openalex.org/W2137518394","https://openalex.org/W2138270253","https://openalex.org/W2149410625","https://openalex.org/W2153508349","https://openalex.org/W2167575072","https://openalex.org/W2168957998","https://openalex.org/W2324373179"],"related_works":["https://openalex.org/W1936277597","https://openalex.org/W1928879541","https://openalex.org/W2583249474","https://openalex.org/W1506578225","https://openalex.org/W1531867071","https://openalex.org/W2291221808","https://openalex.org/W2056474679","https://openalex.org/W122169283","https://openalex.org/W2153792822","https://openalex.org/W2052781717"],"abstract_inverted_index":{"Chemical":[0],"modifications":[1],"to":[2,10,28,101,142,167],"DNA,":[3],"such":[4],"as":[5,192,194,197],"2'":[6],"modifications,":[7],"are":[8,23,63],"expected":[9],"increase":[11],"the":[12,122,143,162,171,185,198],"biotechnological":[13],"utility":[14],"of":[15,21,49,66,93,105,125,136,161,184,188],"DNA;":[16,71],"however,":[17],"these":[18,55,59,78,94,126],"modified":[19,60,70,175],"forms":[20],"DNA":[22,33,43,52],"limited":[24],"by":[25,32],"their":[26],"inability":[27],"be":[29],"effectively":[30],"synthesized":[31],"polymerase":[34,44],"enzymes.":[35,79],"Previous":[36],"efforts":[37],"have":[38],"identified":[39],"mutant":[40,56],"Thermus":[41],"aquaticus":[42],"I":[45],"(Taq)":[46],"enzymes":[47,57],"capable":[48,65],"recognizing":[50],"2'-modified":[51,106,189],"nucleotides.":[53],"While":[54],"recognize":[58,102],"nucleotides,":[61],"they":[62],"not":[64],"synthesizing":[67],"full":[68],"length":[69],"thus,":[72],"further":[73,202],"engineering":[74],"is":[75,99],"required":[76],"for":[77,174,201],"Here,":[80],"we":[81,128],"describe":[82],"comparative":[83],"biochemical":[84],"studies":[85,179],"that":[86,111,139,151],"identify":[87,130,195],"useful,":[88],"but":[89],"previously":[90,112,144],"uncharacterized,":[91],"properties":[92],"enzymes;":[95],"one":[96],"enzyme,":[97],"SFM19,":[98],"able":[100],"a":[103,152],"range":[104],"nucleotides":[107],"much":[108],"wider":[109],"than":[110],"examined,":[113],"including":[114],"fluoro,":[115],"azido,":[116],"and":[117,134,159],"amino":[118,132,137,155],"modifications.":[119],"To":[120],"understand":[121],"molecular":[123],"origins":[124,187],"differences,":[127],"also":[129],"specific":[131],"acids":[133,138],"combinations":[135],"contribute":[140],"most":[141],"evolved":[145],"unnatural":[146],"activity.":[147],"Our":[148],"data":[149],"suggest":[150],"negatively":[153],"charged":[154],"acid":[156],"at":[157],"614":[158],"mutation":[160],"steric":[163],"gate":[164],"residue,":[165],"E615,":[166],"glycine":[168],"make":[169],"up":[170],"optimal":[172],"combination":[173],"oligonucleotide":[176],"synthesis.":[177],"These":[178],"yield":[180],"an":[181],"improved":[182],"understanding":[183],"mutational":[186],"substrate":[190],"recognition":[191],"well":[193],"SFM19":[196],"best":[199],"candidate":[200],"engineering,":[203],"whether":[204],"via":[205],"rational":[206],"design":[207],"or":[208],"directed":[209],"evolution.":[210]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}