{"id":"https://openalex.org/W119905678","doi":"https://doi.org/10.1385/0-89603-332-5:249","title":"A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence","display_name":"A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence","publication_year":2003,"publication_date":"2003-11-14","ids":{"openalex":"https://openalex.org/W119905678","doi":"https://doi.org/10.1385/0-89603-332-5:249","mag":"119905678","pmid":"https://pubmed.ncbi.nlm.nih.gov/8850011"},"language":"en","primary_location":{"id":"doi:10.1385/0-89603-332-5:249","doi":"https://doi.org/10.1385/0-89603-332-5:249","is_oa":false,"landing_page_url":"https://doi.org/10.1385/0-89603-332-5:249","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"In Vitro Mutagenesis Protocols","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W563419041","display_name":"In Vitro Mutagenesis Protocols"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068471387","display_name":"Marjana Tomic\u2010Canic","orcid":"https://orcid.org/0000-0002-9341-0193","observed_orcids":["https://orcid.org/0000-0002-9341-0193"]},"institutions":[{"id":"https://openalex.org/I4210086933","display_name":"NYU Langone Health","ror":"https://ror.org/005dvqh91","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210086933"]},{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marjana Tomic-Canic","raw_affiliation_strings":["Department of Dermatology, New York University Medical Center, NY, USA","Department of Dermatology, New York University Medical Center, New York, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Dermatology, New York University Medical Center, NY, USA","institution_ids":["https://openalex.org/I4210086933","https://openalex.org/I57206974"]},{"raw_affiliation_string":"Department of Dermatology, New York University Medical Center, New York, NY","institution_ids":["https://openalex.org/I4210086933","https://openalex.org/I57206974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075674420","display_name":"Fran\u00e7oise Bernerd","orcid":"https://orcid.org/0000-0001-5426-7948","observed_orcids":["https://orcid.org/0000-0001-5426-7948"]},"institutions":[{"id":"https://openalex.org/I14285755","display_name":"L'Or\u00e9al (France)","ror":"https://ror.org/00nb3j622","country_code":"FR","type":"company","lineage":["https://openalex.org/I14285755"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Fran\u00e7oise Bernerd","raw_affiliation_strings":["L\u2019Or\u00e9al Laboratories, Clichy, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"L\u2019Or\u00e9al Laboratories, Clichy, France","institution_ids":["https://openalex.org/I14285755"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046301485","display_name":"Miroslav Blumenberg","orcid":"https://orcid.org/0000-0002-8672-7774","observed_orcids":["https://orcid.org/0000-0002-8672-7774"]},"institutions":[{"id":"https://openalex.org/I4210086933","display_name":"NYU Langone Health","ror":"https://ror.org/005dvqh91","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210086933"]},{"id":"https://openalex.org/I57206974","display_name":"New York University","ror":"https://ror.org/0190ak572","country_code":"US","type":"education","lineage":["https://openalex.org/I57206974"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Miroslav Blumenberg","raw_affiliation_strings":["Department of Dermatology, New York University Medical Center, New York, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Dermatology, New York University Medical Center, New York, NY","institution_ids":["https://openalex.org/I4210086933","https://openalex.org/I57206974"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.8761,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.71724138,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"57","issue":null,"first_page":"249","last_page":"258"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8130000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8130000233650208,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.13750000298023224,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.023099999874830246,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8949999809265137},{"id":"https://openalex.org/keywords/deletion-mutagenesis","display_name":"deletion mutagenesis","score":0.9449999928474426},{"id":"https://openalex.org/keywords/internal-deletions","display_name":"internal deletions","score":0.9190000295639038}],"concepts":[{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.6747620105743408},{"id":"https://openalex.org/C58301748","wikidata":"https://www.wikidata.org/wiki/Q1074969","display_name":"Restriction site","level":4,"score":0.6458743214607239},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.6022822856903076},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.5911033153533936},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5853173732757568},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5597363710403442},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5395442843437195},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.5387538075447083},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.525542676448822},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.5049870610237122},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.4940994083881378},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.44147759675979614},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2127508521080017},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.08756735920906067}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004274","descriptor_name":"DNA, Recombinant","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017384","descriptor_name":"Sequence Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1385/0-89603-332-5:249","doi":"https://doi.org/10.1385/0-89603-332-5:249","is_oa":false,"landing_page_url":"https://doi.org/10.1385/0-89603-332-5:249","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"In Vitro Mutagenesis Protocols","raw_type":"book-chapter"},{"id":"pmid:8850011","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8850011","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1260410984","display_name":"EPIDERMAL MACROMOLECULAR METABOLISM","funder_award_id":"5r01ar030682-17","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G2004671243","display_name":"REGULATION OF KERATINIZATION BY HORMONES AND VITAMINS","funder_award_id":"5r01ar040522-04","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G2011479232","display_name":"SIGNAL TRANSDUCTION MECHANISMS IN EPIDERMIS","funder_award_id":"R01AR041850","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G4331937614","display_name":null,"funder_award_id":"AR 30682","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G5629099154","display_name":null,"funder_award_id":"AR-41850","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"},{"id":"https://openalex.org/G6840693146","display_name":null,"funder_award_id":"AR40522","funder_id":"https://openalex.org/F4320337362","funder_display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337362","display_name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","ror":"https://ror.org/006zn3t30"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W1497850848","https://openalex.org/W1990663996","https://openalex.org/W1991214910","https://openalex.org/W2033491152","https://openalex.org/W2034699600","https://openalex.org/W2415074568"],"related_works":["https://openalex.org/W2954878838","https://openalex.org/W2416245391","https://openalex.org/W2014578950","https://openalex.org/W260317786","https://openalex.org/W2056384492","https://openalex.org/W7124417608","https://openalex.org/W1580605464","https://openalex.org/W2034071213","https://openalex.org/W2070591676","https://openalex.org/W2358175167"],"abstract_inverted_index":{"Site-directed":[0],"mutagenesis":[1,43],"is":[2,142],"a":[3,29,37,54,66,82,116],"powerful":[4],"tool":[5,119],"used":[6],"in":[7,65],"modern":[8],"molecular":[9],"biology":[10],"for":[11,41,57,85,120,122],"protein":[12],"and":[13,20,135,144],"genetic":[14],"engineering.":[15],"A":[16],"number":[17],"of":[18,74,93,104,109],"simple":[19,55],"elegant":[21],"protocols":[22],"are":[23,35],"available":[24],"to":[25,78],"introduce":[26],"mutations":[27],"into":[28,61,97],"target":[30,67],"DNA":[31,68,76,95],"sequence.":[32,69],"However,":[33],"there":[34],"only":[36],"few":[38],"methods":[39],"described":[40],"deletion":[42],"(":[44],"1":[45],"\u2013":[46],"4":[47],").":[48],"In":[49],"this":[50],"chapter,":[51],"we":[52],"demonstrate":[53],"method":[56],"creating":[58],"internal":[59],"deletions":[60],"any":[62],"desired":[63,102],"position":[64],"The":[70],"procedure":[71],"involves":[72],"replacement":[73],"the":[75,91,98,105,110,126,138,145,151],"region":[77],"be":[79,148],"deleted":[80,106],"with":[81],"restriction":[83,112],"site":[84,99,113],"an":[86],"endonuclease.":[87],"This":[88,140],"also":[89,114],"facilitates":[90],"insertion":[92],"other":[94],"sequences":[96],"or":[100],"if":[101],"restoration":[103],"fragment.":[107],"Introduction":[108],"new":[111],"provides":[115],"useful":[117],"diagnostic":[118],"screening":[121],"positive":[123],"clones":[124],"following":[125],"transformation":[127],"procedure.":[128],"These":[129],"keywords":[130,146],"were":[131],"added":[132],"by":[133,137],"machine":[134],"not":[136],"authors.":[139],"process":[141],"experimental":[143],"may":[147],"updated":[149],"as":[150],"learning":[152],"algorithm":[153],"improves.":[154]},"counts_by_year":[{"year":2017,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
