{"id":"https://openalex.org/W118931272","doi":"https://doi.org/10.1007/978-1-60327-029-8_9","title":"Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes","display_name":"Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes","publication_year":2009,"publication_date":"2009-11-25","ids":{"openalex":"https://openalex.org/W118931272","doi":"https://doi.org/10.1007/978-1-60327-029-8_9","mag":"118931272","pmid":"https://pubmed.ncbi.nlm.nih.gov/20013177","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3085892"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-60327-029-8_9","doi":"https://doi.org/10.1007/978-1-60327-029-8_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60327-029-8_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W619228734","display_name":"Free Radicals and Antioxidant Protocols"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3085892","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109843020","display_name":"Eric A. Simone","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I61970326","display_name":"Vertex Pharmaceuticals (United States)","ror":"https://ror.org/00anb1726","country_code":"US","type":"company","lineage":["https://openalex.org/I61970326"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Eric Simone","raw_affiliation_strings":["Vertex Pharmaceuticals, Inc, Formulation Development, 130 Waverly Street, Cambridge, MA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vertex Pharmaceuticals, Inc, Formulation Development, 130 Waverly Street, Cambridge, MA, USA","institution_ids":["https://openalex.org/I61970326"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051709163","display_name":"Thomas D. Dziubla","orcid":"https://orcid.org/0000-0003-2518-3926","observed_orcids":["https://orcid.org/0000-0003-2518-3926"]},"institutions":[{"id":"https://openalex.org/I143302722","display_name":"University of Kentucky","ror":"https://ror.org/02k3smh20","country_code":"US","type":"education","lineage":["https://openalex.org/I143302722"]},{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas Dziubla","raw_affiliation_strings":["Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY, USA","Department of Pharmacology and Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical and Materials Engineering, University of Kentucky, Lexington, KY, USA","institution_ids":["https://openalex.org/I143302722"]},{"raw_affiliation_string":"Department of Pharmacology and Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038786186","display_name":"Vladimir V. Shuvaev","orcid":"https://orcid.org/0000-0003-2449-460X","observed_orcids":["https://orcid.org/0000-0003-2449-460X"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vladimir Shuvaev","raw_affiliation_strings":["Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5022924802","display_name":"Vladimir R. Muzykantov","orcid":"https://orcid.org/0000-0001-6648-1204","observed_orcids":["https://orcid.org/0000-0001-6648-1204"]},"institutions":[{"id":"https://openalex.org/I79576946","display_name":"University of Pennsylvania","ror":"https://ror.org/00b30xv10","country_code":"US","type":"education","lineage":["https://openalex.org/I79576946"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vladimir R. Muzykantov","raw_affiliation_strings":["Department of Pharmacology, Institute for Environmental Medicine, and Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, Institute for Environmental Medicine, and Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA","institution_ids":["https://openalex.org/I79576946"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.8249,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.70022883,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"610","issue":null,"first_page":"145","last_page":"164"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10920","display_name":"Advanced Drug Delivery Systems","score":0.00570000009611249,"subfield":{"id":"https://openalex.org/subfields/3003","display_name":"Pharmaceutical Science"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/polymeric-nanocarriers","display_name":"polymeric nanocarriers","score":0.9980000257492065},{"id":"https://openalex.org/keywords/catalase","display_name":"catalase","score":0.9089999794960022},{"id":"https://openalex.org/keywords/protein-drug-delivery","display_name":"protein drug delivery","score":0.7089999914169312},{"id":"https://openalex.org/keywords/protein-encapsulation","display_name":"protein encapsulation","score":0.5440000295639038},{"id":"https://openalex.org/keywords/endothelial-targeting","display_name":"endothelial targeting","score":0.8040000200271606},{"id":"https://openalex.org/keywords/targeting-ligands","display_name":"targeting ligands","score":0.6710000038146973},{"id":"https://openalex.org/keywords/antioxidant-enzymes","display_name":"antioxidant enzymes","score":0.6000000238418579},{"id":"https://openalex.org/keywords/recombinant-enzyme","display_name":"recombinant enzyme","score":0.6690000295639038},{"id":"https://openalex.org/keywords/subcellular-localization","display_name":"subcellular localization","score":0.6650000214576721}],"concepts":[{"id":"https://openalex.org/C18150654","wikidata":"https://www.wikidata.org/wiki/Q17153796","display_name":"Nanocarriers","level":3,"score":0.8939799070358276},{"id":"https://openalex.org/C126511603","wikidata":"https://www.wikidata.org/wiki/Q1261145","display_name":"Targeted drug delivery","level":3,"score":0.5175877809524536},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.513626754283905},{"id":"https://openalex.org/C2779820397","wikidata":"https://www.wikidata.org/wiki/Q1392806","display_name":"Drug delivery","level":2,"score":0.5063732862472534},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.48125049471855164},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.31693077087402344}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002374","descriptor_name":"Catalase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002374","descriptor_name":"Catalase","qualifier_ui":"Q000627","qualifier_name":"therapeutic use","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004337","descriptor_name":"Drug Carriers","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":true},{"descriptor_ui":"D004337","descriptor_name":"Drug Carriers","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004337","descriptor_name":"Drug Carriers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010316","descriptor_name":"Particle Size","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":true},{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011108","descriptor_name":"Polymers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015815","descriptor_name":"Cell Adhesion Molecules","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016503","descriptor_name":"Drug Delivery Systems","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D042783","descriptor_name":"Endothelial Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D042783","descriptor_name":"Endothelial Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049329","descriptor_name":"Nanostructures","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1007/978-1-60327-029-8_9","doi":"https://doi.org/10.1007/978-1-60327-029-8_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60327-029-8_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:20013177","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20013177","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3085892","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3085892","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:3085892","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3085892","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7963607731","display_name":"Delivery of Anti-thrombotic Drugs by Blood Cells","funder_award_id":"R01HL090697","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G8290060235","display_name":"Targeting Carriers with Controlled Geometry to Endothelium","funder_award_id":"5r01hl087036-05","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G922908772","display_name":null,"funder_award_id":"R01-HL087036","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W171163714","https://openalex.org/W186628991","https://openalex.org/W1865604683","https://openalex.org/W1966614349","https://openalex.org/W1989164106","https://openalex.org/W1993029332","https://openalex.org/W2002516695","https://openalex.org/W2015627525","https://openalex.org/W2023947408","https://openalex.org/W2029749273","https://openalex.org/W2033297392","https://openalex.org/W2035316083","https://openalex.org/W2043684255","https://openalex.org/W2046634017","https://openalex.org/W2050061574","https://openalex.org/W2057110063","https://openalex.org/W2058693442","https://openalex.org/W2100225360","https://openalex.org/W2102436727","https://openalex.org/W2104485020","https://openalex.org/W2114494901","https://openalex.org/W2147892054","https://openalex.org/W2152008647","https://openalex.org/W2152574565","https://openalex.org/W2158038344","https://openalex.org/W2162703233","https://openalex.org/W2171792381","https://openalex.org/W2402177936","https://openalex.org/W2411350820","https://openalex.org/W2418433448","https://openalex.org/W2463994390","https://openalex.org/W4212814362","https://openalex.org/W4251403035","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2155785002","https://openalex.org/W1989164106","https://openalex.org/W2694326366","https://openalex.org/W3111480685","https://openalex.org/W4320724872","https://openalex.org/W1993029332","https://openalex.org/W2024016899","https://openalex.org/W4251762736","https://openalex.org/W146019667","https://openalex.org/W4383620613"],"abstract_inverted_index":{"Protein":[0],"drugs,":[1],"such":[2],"as":[3],"recombinant":[4],"enzymes":[5],"useful":[6],"for":[7,86,159],"detoxification":[8],"and":[9,15,24,71,99,117,124,131],"replacement":[10],"therapies,":[11],"have":[12],"extraordinary":[13],"specificity":[14],"potency.":[16],"However,":[17],"inherently":[18],"inadequate":[19],"delivery":[20],"to":[21,101,139],"target":[22],"sites":[23],"rapid":[25],"inactivation":[26,53],"limit":[27],"their":[28],"medical":[29],"utility.":[30],"Using":[31],"chaperone":[32],"polymeric":[33],"particles":[34],"designed":[35],"within":[36],"an":[37],"injectible":[38],"size":[39],"range":[40],"(sub-micron)":[41],"may":[42],"help":[43],"solve":[44],"these":[45,152],"shortcomings.":[46],"Such":[47],"nanocarriers":[48,91],"would":[49],"(i)":[50],"prevent":[51],"premature":[52],"of":[54,76,89,105,113,121,133,143,162,166],"encapsulated":[55],"therapeutic":[56],"protein":[57],"cargoes,":[58],"(ii)":[59],"provide":[60,155],"a":[61,114,156,163],"carrier":[62],"that":[63,151],"can":[64],"be":[65],"surface":[66],"decorated":[67],"by":[68],"targeting":[69,115,125,145,169],"ligands,":[70],"(iii)":[72],"optimize":[73],"sub-cellular":[74],"localization":[75],"the":[77,82,87,95,122,140,144,160],"drug.":[78],"This":[79],"chapter":[80],"describes":[81],"techniques":[83],"successfully":[84],"employed":[85],"preparation":[88],"polymer":[90],"(PNC)":[92],"loaded":[93],"with":[94,109],"antioxidant":[96],"enzyme,":[97],"catalase,":[98,110],"targeted":[100],"endothelial":[102],"cells.":[103],"Methods":[104],"PNC":[106],"synthesis,":[107],"loading":[108],"characterization,":[111],"coupling":[112],"moiety,":[116],"in":[118],"vitro":[119],"testing":[120],"enzymatic":[123,167],"activities":[126],"are":[127,136],"provided":[128],"here.":[129],"Advantages":[130],"disadvantages":[132],"specific":[134],"designs":[135],"discussed.":[137],"Due":[138],"modular":[141],"nature":[142],"methodology":[146],"employed,":[147],"it":[148],"is":[149],"believed":[150],"protocols":[153],"will":[154],"solid":[157],"foundation":[158],"formulation":[161],"wide":[164],"variety":[165],"drug":[168],"strategies.":[170]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}