{"id":"https://openalex.org/W117701556","doi":"https://doi.org/10.2144/99262rr04","title":"Organelle Isolation by Magnetic Immunoabsorption","display_name":"Organelle Isolation by Magnetic Immunoabsorption","publication_year":1999,"publication_date":"1999-02-01","ids":{"openalex":"https://openalex.org/W117701556","doi":"https://doi.org/10.2144/99262rr04","mag":"117701556","pmid":"https://pubmed.ncbi.nlm.nih.gov/10023546"},"language":"en","primary_location":{"id":"doi:10.2144/99262rr04","doi":"https://doi.org/10.2144/99262rr04","is_oa":true,"landing_page_url":"https://doi.org/10.2144/99262rr04","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/99262rr04","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016106266","display_name":"Albert Paul Kausch","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I140172145","display_name":"University of Connecticut","ror":"https://ror.org/02der9h97","country_code":"US","type":"education","lineage":["https://openalex.org/I140172145"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Albert P. Kausch","raw_affiliation_strings":["University of Connecticut, Storrs"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Connecticut, Storrs","institution_ids":["https://openalex.org/I140172145"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032018703","display_name":"T. Page Owen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4498119","display_name":"Connecticut College","ror":"https://ror.org/01hpqfm28","country_code":"US","type":"education","lineage":["https://openalex.org/I4498119"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T. Page Owen","raw_affiliation_strings":["Connecticut College, New London, CT"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Connecticut College, New London, CT","institution_ids":["https://openalex.org/I4498119"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015037175","display_name":"Sandya Narayanswami","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210115256","display_name":"Jackson Laboratory","ror":"https://ror.org/021sy4w91","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210115256"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S. Narayanswami","raw_affiliation_strings":["The Jackson Laboratory, Bar Harbor, ME"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Jackson Laboratory, Bar Harbor, ME","institution_ids":["https://openalex.org/I4210115256"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047465035","display_name":"Barry D. Bruce","orcid":"https://orcid.org/0000-0002-4045-9815","observed_orcids":["https://orcid.org/0000-0002-4045-9815"]},"institutions":[{"id":"https://openalex.org/I75027704","display_name":"University of Tennessee at Knoxville","ror":"https://ror.org/020f3ap87","country_code":"US","type":"education","lineage":["https://openalex.org/I75027704"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Barry D. Bruce","raw_affiliation_strings":["University of Tennessee, Knoxville, TN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Tennessee, Knoxville, TN, USA","institution_ids":["https://openalex.org/I75027704"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5047465035"],"corresponding_institution_ids":["https://openalex.org/I75027704"],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":0.6197,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.59231286,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"26","issue":"2","first_page":"336","last_page":"343"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.23010000586509705,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.23010000586509705,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.1753000020980835,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.057999998331069946,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/organelle-isolation","display_name":"organelle isolation","score":0.9819999933242798},{"id":"https://openalex.org/keywords/immunolabeling","display_name":"immunolabeling","score":0.8759999871253967},{"id":"https://openalex.org/keywords/plant-organelles","display_name":"plant organelles","score":0.8140000104904175},{"id":"https://openalex.org/keywords/amyloplasts","display_name":"amyloplasts","score":0.8939999938011169},{"id":"https://openalex.org/keywords/chloroplasts","display_name":"chloroplasts","score":0.9950000047683716},{"id":"https://openalex.org/keywords/nuclei","display_name":"nuclei","score":0.5640000104904175},{"id":"https://openalex.org/keywords/mitotic-chromosomes","display_name":"metaphase chromosomes","score":0.8679999709129333},{"id":"https://openalex.org/keywords/magnetic-separation","display_name":"magnetic separation","score":0.5149999856948853}],"concepts":[{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.9284572601318359},{"id":"https://openalex.org/C2777877921","wikidata":"https://www.wikidata.org/wiki/Q6005532","display_name":"Immunolabeling","level":3,"score":0.5980588793754578},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5270925760269165},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.4909959137439728},{"id":"https://openalex.org/C69305403","wikidata":"https://www.wikidata.org/wiki/Q47263","display_name":"Chloroplast","level":3,"score":0.41484537720680237},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4142614006996155},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22949695587158203},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000939","descriptor_name":"Epitopes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001710","descriptor_name":"Biotin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002458","descriptor_name":"Cell Fractionation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D002736","descriptor_name":"Chloroplasts","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002736","descriptor_name":"Chloroplasts","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D002875","descriptor_name":"Chromosomes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010940","descriptor_name":"Plant Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018189","descriptor_name":"Immunomagnetic Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018385","descriptor_name":"Centrosome","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D018385","descriptor_name":"Centrosome","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018744","descriptor_name":"DNA, Plant","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D019809","descriptor_name":"Streptavidin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.2144/99262rr04","doi":"https://doi.org/10.2144/99262rr04","is_oa":true,"landing_page_url":"https://doi.org/10.2144/99262rr04","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:10023546","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10023546","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:5812026c14b24110ae9ad349f03cae97","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/5812026c14b24110ae9ad349f03cae97","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 26, Iss 2, Pp 336-343 (1999)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/99262rr04","doi":"https://doi.org/10.2144/99262rr04","is_oa":true,"landing_page_url":"https://doi.org/10.2144/99262rr04","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":9,"referenced_works":["https://openalex.org/W103530912","https://openalex.org/W625681974","https://openalex.org/W1964444845","https://openalex.org/W1999694830","https://openalex.org/W2030664216","https://openalex.org/W2032588325","https://openalex.org/W2090854251","https://openalex.org/W2096155855","https://openalex.org/W2153732202"],"related_works":["https://openalex.org/W2185836096","https://openalex.org/W2905643967","https://openalex.org/W2478770681","https://openalex.org/W7099008702","https://openalex.org/W2948099654","https://openalex.org/W4376138068","https://openalex.org/W1980824487","https://openalex.org/W4243300032","https://openalex.org/W3127678150","https://openalex.org/W4376144015"],"abstract_inverted_index":{"Currently,":[0],"most":[1],"organelle":[2,100],"isolation":[3,20],"procedures":[4],"rely":[5],"on":[6],"physical":[7],"parameters":[8],"and":[9,18,34,58,67,86,122],"centrifugation":[10],"for":[11,99,114,133],"separation.":[12],"Here,":[13],"we":[14,48,109],"report":[15],"the":[16,80],"rapid":[17],"gentle":[19],"of":[21,24,61,73,90,117,126],"a":[22,42,59,112,124],"variety":[23,60,125],"organelles":[25,119],"by":[26,39],"immunolabeling":[27],"whole":[28,70],"cell":[29,71],"lysates":[30,72],"with":[31],"organelle-specific":[32],"antibodies":[33,127],"streptavidin":[35],"magnetic":[36,43,46,82,106],"particles":[37],"followed":[38],"separation":[40,107],"in":[41,120],"field.":[44],"Using":[45],"immunoabsorption,":[47],"have":[49,110],"been":[50],"able":[51],"to":[52,128,136],"specifically":[53],"label":[54],"mouse":[55],"metaphase":[56],"chromosomes":[57],"plant":[62,75],"organelles,":[63],"including:":[64],"amyloplasts,":[65],"choroplasts":[66],"nuclei":[68],"from":[69],"various":[74],"tissues.":[76],"We":[77],"find":[78],"that":[79],"distinct":[81],"properties,":[83],"surface":[84],"characteristics":[85],"mean":[87],"diameter-size":[88],"ranges":[89],"different":[91],"particle":[92],"preparations":[93],"significantly":[94],"influence":[95],"their":[96,134],"specific":[97],"utility":[98],"isolations.":[101],"By":[102],"using":[103],"an":[104],"internal-field":[105],"device,":[108],"developed":[111],"method":[113],"quantitative":[115],"recovery":[116],"labeled":[118],"microarrays":[121],"tested":[123],"chloroplast":[129],"outer":[130],"envelope":[131],"proteins":[132],"ability":[135],"immune-isolate":[137],"chloroplasts.":[138]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}