{"id":"https://openalex.org/W1166471076","doi":"https://doi.org/10.1016/s0076-6879(83)02005-4","title":"[3] Purification of calmodulin by Ca2+-dependent affinity chromatography","display_name":"[3] Purification of calmodulin by Ca2+-dependent affinity chromatography","publication_year":1983,"publication_date":"1983-01-01","ids":{"openalex":"https://openalex.org/W1166471076","doi":"https://doi.org/10.1016/s0076-6879(83)02005-4","mag":"1166471076","pmid":"https://pubmed.ncbi.nlm.nih.gov/6645969"},"language":"en","primary_location":{"id":"doi:10.1016/s0076-6879(83)02005-4","doi":"https://doi.org/10.1016/s0076-6879(83)02005-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0076-6879(83)02005-4","pdf_url":null,"source":{"id":"https://openalex.org/S182211142","display_name":"Methods in enzymology on CD-ROM/Methods in enzymology","issn_l":"0076-6879","issn":["0076-6879","1079-2376","1557-7988"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319955","host_organization_name":"Academic Press","host_organization_lineage":["https://openalex.org/P4310319955","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Academic Press","Elsevier BV"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Enzymology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4236365517","display_name":"Hormone Action Part G"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005668168","display_name":"Harry Charbonneau","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Harry Charbonneau","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060902845","display_name":"Rita E. Hice","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Rita Hice","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080438193","display_name":"Russell C. Hart","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Russell C. Hart","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111863772","display_name":"Milton J. Cormier","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Milton J. Cormier","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.7665,"has_fulltext":false,"cited_by_count":46,"citation_normalized_percentile":{"value":0.9218466,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"102","issue":null,"first_page":"17","last_page":"39"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.6840999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.6840999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12030","display_name":"S100 Proteins and Annexins","score":0.18289999663829803,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.029899999499320984,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/calmodulin","display_name":"calmodulin","score":0.996999979019165},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.8600000143051147},{"id":"https://openalex.org/keywords/ammonium-sulfate-precipitation","display_name":"ammonium sulfate precipitation","score":0.8550000190734863},{"id":"https://openalex.org/keywords/deae-cellulose-chromatography","display_name":"DEAE-cellulose chromatography","score":0.8669999837875366},{"id":"https://openalex.org/keywords/trichloroacetic-acid-precipitation","display_name":"trichloroacetic acid precipitation","score":0.996999979019165},{"id":"https://openalex.org/keywords/ion-exchange-chromatography","display_name":"ion-exchange chromatography","score":0.7770000100135803},{"id":"https://openalex.org/keywords/pollutant-removal","display_name":"pollutant removal","score":0.5820000171661377}],"concepts":[{"id":"https://openalex.org/C29688787","wikidata":"https://www.wikidata.org/wiki/Q409145","display_name":"Calmodulin","level":3,"score":0.9117021560668945},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.7613200545310974},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6469160318374634},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6152337193489075},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2525654733181},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.07094106078147888}],"mesh":[{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002147","descriptor_name":"Calmodulin","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D002621","descriptor_name":"Chemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D003488","descriptor_name":"Cyanogen Bromide","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004355","descriptor_name":"Drug Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004852","descriptor_name":"Epoxy Compounds","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010640","descriptor_name":"Phenothiazines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012685","descriptor_name":"Sepharose","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D013053","descriptor_name":"Spectrophotometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055598","descriptor_name":"Chemical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0076-6879(83)02005-4","doi":"https://doi.org/10.1016/s0076-6879(83)02005-4","is_oa":false,"landing_page_url":"https://doi.org/10.1016/s0076-6879(83)02005-4","pdf_url":null,"source":{"id":"https://openalex.org/S182211142","display_name":"Methods in enzymology on CD-ROM/Methods in enzymology","issn_l":"0076-6879","issn":["0076-6879","1079-2376","1557-7988"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319955","host_organization_name":"Academic Press","host_organization_lineage":["https://openalex.org/P4310319955","https://openalex.org/P4310320990"],"host_organization_lineage_names":["Academic Press","Elsevier BV"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Enzymology","raw_type":"book-chapter"},{"id":"pmid:6645969","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6645969","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in enzymology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W1497854461","https://openalex.org/W1503766585","https://openalex.org/W1505846226","https://openalex.org/W1512693490","https://openalex.org/W1546458288","https://openalex.org/W1554038443","https://openalex.org/W1566181557","https://openalex.org/W1599390139","https://openalex.org/W1846068880","https://openalex.org/W1917093452","https://openalex.org/W1933373536","https://openalex.org/W1961025744","https://openalex.org/W1979197184","https://openalex.org/W1986358965","https://openalex.org/W1990262095","https://openalex.org/W1995135269","https://openalex.org/W2002204004","https://openalex.org/W2003458744","https://openalex.org/W2030669640","https://openalex.org/W2040527936","https://openalex.org/W2043775715","https://openalex.org/W2047848482","https://openalex.org/W2058644682","https://openalex.org/W2059782931","https://openalex.org/W2062947813","https://openalex.org/W2063655141","https://openalex.org/W2067554838","https://openalex.org/W2068677180","https://openalex.org/W2077772799","https://openalex.org/W2080659849","https://openalex.org/W2089327959","https://openalex.org/W2092315848","https://openalex.org/W2253302896","https://openalex.org/W2331975755","https://openalex.org/W2409521202","https://openalex.org/W2410835544","https://openalex.org/W2469078899","https://openalex.org/W2470869994","https://openalex.org/W4299841803","https://openalex.org/W4406986155"],"related_works":["https://openalex.org/W2092315848","https://openalex.org/W2059782931","https://openalex.org/W1986358965","https://openalex.org/W1199898208","https://openalex.org/W1994854431","https://openalex.org/W1831722528","https://openalex.org/W1997883722","https://openalex.org/W1426024167","https://openalex.org/W2793497013","https://openalex.org/W2071056369"],"abstract_inverted_index":null,"counts_by_year":[{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}