{"id":"https://openalex.org/W114089253","doi":null,"title":"Characterization of natural killer cells cultured from human bone marrow cells.","display_name":"Characterization of natural killer cells cultured from human bone marrow cells.","publication_year":1988,"publication_date":"1988-09-01","ids":{"openalex":"https://openalex.org/W114089253","mag":"114089253","pmid":"https://pubmed.ncbi.nlm.nih.gov/3042444"},"language":"en","primary_location":{"id":"pmid:3042444","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3042444","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental hematology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5054350597","display_name":"Yasuhiro Yoda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Y Yoda","raw_affiliation_strings":["Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan","institution_ids":["https://openalex.org/I146399215"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Z Kawakami","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Z Kawakami","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083746251","display_name":"Akira Shibuya","orcid":"https://orcid.org/0000-0002-4480-4858","observed_orcids":["https://orcid.org/0000-0002-4480-4858"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Shibuya","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5072517591","display_name":"Tsukasa Abe","orcid":"https://orcid.org/0000-0002-2400-3797","observed_orcids":["https://orcid.org/0000-0002-2400-3797"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T Abe","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4381,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.57850999,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"16","issue":"8","first_page":"712","last_page":"7"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11020","display_name":"Immune Cell Function and Interaction","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11020","display_name":"Immune Cell Function and Interaction","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11313","display_name":"Immune cells in cancer","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/natural-killer-cells","display_name":"natural killer cells","score":0.9940000176429749},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.6629999876022339},{"id":"https://openalex.org/keywords/nk-cell-differentiation","display_name":"NK cell differentiation","score":0.7120000123977661},{"id":"https://openalex.org/keywords/interleukin-2","display_name":"interleukin-2","score":0.7749999761581421},{"id":"https://openalex.org/keywords/large-granular-lymphocytes","display_name":"large granular lymphocytes","score":0.8690000176429749},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.7039999961853027},{"id":"https://openalex.org/keywords/k562-cells","display_name":"K562 cells","score":0.8550000190734863},{"id":"https://openalex.org/keywords/dna-synthesis","display_name":"DNA synthesis","score":0.6589999794960022},{"id":"https://openalex.org/keywords/bromodeoxyuridine","display_name":"bromodeoxyuridine","score":0.8119999766349792},{"id":"https://openalex.org/keywords/ultraviolet-radiation","display_name":"ultraviolet radiation","score":0.7680000066757202},{"id":"https://openalex.org/keywords/surface-phenotype","display_name":"surface phenotype","score":0.4659999907016754},{"id":"https://openalex.org/keywords/progenitor-cells","display_name":"progenitor cells","score":0.5699999928474426}],"concepts":[{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.7461024522781372},{"id":"https://openalex.org/C171122931","wikidata":"https://www.wikidata.org/wiki/Q6324626","display_name":"K562 cells","level":3,"score":0.7375447154045105},{"id":"https://openalex.org/C149532602","wikidata":"https://www.wikidata.org/wiki/Q2409681","display_name":"Interleukin 12","level":4,"score":0.6323659420013428},{"id":"https://openalex.org/C129374314","wikidata":"https://www.wikidata.org/wiki/Q909381","display_name":"Interleukin 21","level":4,"score":0.5877071619033813},{"id":"https://openalex.org/C2781462264","wikidata":"https://www.wikidata.org/wiki/Q78482053","display_name":"CD16","level":5,"score":0.5847795605659485},{"id":"https://openalex.org/C28651165","wikidata":"https://www.wikidata.org/wiki/Q4259786","display_name":"Lymphokine-activated killer cell","level":5,"score":0.5822676420211792},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5732384324073792},{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.5192677974700928},{"id":"https://openalex.org/C114684123","wikidata":"https://www.wikidata.org/wiki/Q419458","display_name":"CD3","level":4,"score":0.5065444707870483},{"id":"https://openalex.org/C139563560","wikidata":"https://www.wikidata.org/wiki/Q224930","display_name":"Natural killer T cell","level":4,"score":0.48705533146858215},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.46355026960372925},{"id":"https://openalex.org/C206191192","wikidata":"https://www.wikidata.org/wiki/Q17149636","display_name":"NK-92","level":5,"score":0.4520657956600189},{"id":"https://openalex.org/C2778102761","wikidata":"https://www.wikidata.org/wiki/Q332181","display_name":"Natural killer cell","level":4,"score":0.4520355463027954},{"id":"https://openalex.org/C131665280","wikidata":"https://www.wikidata.org/wiki/Q14866169","display_name":"Interleukin 3","level":5,"score":0.42644521594047546},{"id":"https://openalex.org/C167672396","wikidata":"https://www.wikidata.org/wiki/Q417800","display_name":"CD8","level":3,"score":0.4027726352214813},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3431682586669922},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.2808016240596771},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.19323745369911194},{"id":"https://openalex.org/C2778461978","wikidata":"https://www.wikidata.org/wiki/Q29496","display_name":"Leukemia","level":2,"score":0.13892969489097595},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.09934252500534058}],"mesh":[{"descriptor_ui":"D000954","descriptor_name":"Antigens, Surface","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001853","descriptor_name":"Bone Marrow","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001854","descriptor_name":"Bone Marrow Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001973","descriptor_name":"Bromodeoxyuridine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003602","descriptor_name":"Cytotoxicity, Immunologic","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D003602","descriptor_name":"Cytotoxicity, Immunologic","qualifier_ui":"Q000528","qualifier_name":"radiation effects","is_major_topic":false},{"descriptor_ui":"D004261","descriptor_name":"DNA Replication","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004261","descriptor_name":"DNA Replication","qualifier_ui":"Q000528","qualifier_name":"radiation effects","is_major_topic":false},{"descriptor_ui":"D005455","descriptor_name":"Fluorescent Antibody Technique","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007694","descriptor_name":"Killer Cells, Natural","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D007694","descriptor_name":"Killer Cells, Natural","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D007694","descriptor_name":"Killer Cells, Natural","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014466","descriptor_name":"Ultraviolet Rays","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:3042444","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3042444","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental hematology","raw_type":"Journal Article"},{"id":"mag:114089253","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3042444","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1660408415","https://openalex.org/W205667059","https://openalex.org/W111474836","https://openalex.org/W1529205544","https://openalex.org/W2418258997","https://openalex.org/W2395694643","https://openalex.org/W2418723614","https://openalex.org/W2006715896","https://openalex.org/W2030305351","https://openalex.org/W2400066600"],"abstract_inverted_index":{"Human":[0],"bone":[1],"marrow":[2],"(BM)":[3],"cells,":[4,9,11,16,159],"depleted":[5],"of":[6,53,58,68,129,157],"nylon":[7],"wool-adherent":[8],"T":[10],"and":[12,40,64,97],"natural":[13],"killer":[14],"(NK)":[15],"were":[17,49,92,107,117],"cultured":[18,89],"in":[19,31,110],"medium":[20],"containing":[21],"recombinant":[22],"interleukin":[23],"2":[24],"(rIL2).":[25],"After":[26],"21":[27],"or":[28,78],"24":[29],"days":[30],"culture,":[32],"numerous":[33],"lymphoid":[34],"cells":[35,60,69,91,106,124],"with":[36,86],"multiple":[37],"azurophilic":[38],"granules":[39],"a":[41,65],"morphology":[42],"similar":[43],"to":[44,61,112,119],"large":[45],"granular":[46],"lymphocytes":[47],"(LGL)":[48],"found.":[50],"Two-color":[51],"analysis":[52],"surface":[54],"phenotype":[55],"showed":[56],"many":[57],"these":[59],"be":[62,120],"NKH1-positive":[63],"limited":[66],"number":[67],"had":[70],"other":[71],"NK":[72,165],"markers":[73],"such":[74],"as":[75],"CD16,":[76],"CD2,":[77],"CD8.":[79],"The":[80,88,101,127],"CD3":[81],"antigen":[82],"was":[83,132],"not":[84],"coexpressed":[85],"NKH1.":[87],"BM":[90],"cytotoxic":[93,121],"for":[94,122],"K562,":[95],"Daudi,":[96],"Raji":[98],"cell":[99],"lines.":[100],"NKH1+,":[102],"CD2-,":[103],"CD3-,":[104],"CD16-":[105],"sorted":[108],"and,":[109],"addition":[111],"having":[113],"the":[114,151],"LGL":[115],"morphology,":[116],"found":[118],"K562":[123],"(NK":[125],"[K562]).":[126],"generation":[128],"NK(K562)":[130],"activity":[131],"significantly":[133],"suppressed":[134],"by":[135],"5-bromodeoxyuridine":[136],"plus":[137],"ultraviolet":[138],"light":[139],"treatment,":[140],"indicating":[141],"that":[142,150],"DNA":[143],"synthesis":[144],"is":[145],"required.":[146],"These":[147],"experiments":[148],"suggest":[149],"described":[152],"culture":[153],"conditions":[154],"allow":[155],"differentiation":[156],"progenitor":[158],"into":[160],"immature,":[161],"but":[162],"functionally":[163],"active,":[164],"cells.":[166]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}