{"id":"https://openalex.org/W11263578","doi":null,"title":"Effect of cysteine point mutation to tryptophan on the catalytic activity of aldehyde dehydrogenase","display_name":"Effect of cysteine point mutation to tryptophan on the catalytic activity of aldehyde dehydrogenase","publication_year":1991,"publication_date":"1991-03-11","ids":{"openalex":"https://openalex.org/W11263578","mag":"11263578"},"language":"en","primary_location":{"id":"mag:11263578","doi":null,"is_oa":false,"landing_page_url":"http://www.osti.gov/scitech/biblio/5373546-effect-cysteine-point-mutation-tryptophan-catalytic-activity-aldehyde-dehydrogenase","pdf_url":null,"source":{"id":"https://openalex.org/S4306510356","display_name":"FASEB Journal (Federation of American Societies for Experimental Biology); (United States)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"FASEB Journal (Federation of American Societies for Experimental Biology); (United States)","raw_type":null},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072413775","display_name":"G. Ghenbot","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G. Ghenbot","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5012958448","display_name":"Henry Weiner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Henry Weiner","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0011884,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.7045999765396118,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.7045999765396118,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12412","display_name":"Enzyme function and inhibition","score":0.0625,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.03400000184774399,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aldehyde-dehydrogenase","display_name":"aldehyde dehydrogenase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/active-site-residues","display_name":"active site residues","score":0.7570000290870667},{"id":"https://openalex.org/keywords/n-ethylmaleimide-modification","display_name":"N-ethylmaleimide modification","score":0.7879999876022339},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.7269999980926514},{"id":"https://openalex.org/keywords/affinity-chromatography","display_name":"affinity chromatography","score":0.597000002861023},{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.7149999737739563},{"id":"https://openalex.org/keywords/pulse-chase-labeling","display_name":"pulse-chase labeling","score":0.7200000286102295}],"concepts":[{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.675939679145813},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.6482723951339722},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.635837197303772},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6276991963386536},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6259627342224121},{"id":"https://openalex.org/C2778886173","wikidata":"https://www.wikidata.org/wiki/Q53832","display_name":"Tetramer","level":3,"score":0.5863826870918274},{"id":"https://openalex.org/C25747580","wikidata":"https://www.wikidata.org/wiki/Q423792","display_name":"Aldehyde dehydrogenase","level":3,"score":0.539236307144165},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.4928905963897705},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.46121203899383545},{"id":"https://openalex.org/C2776706248","wikidata":"https://www.wikidata.org/wiki/Q181003","display_name":"Tryptophan","level":3,"score":0.45818203687667847},{"id":"https://openalex.org/C2781338088","wikidata":"https://www.wikidata.org/wiki/Q903495","display_name":"Residue (chemistry)","level":2,"score":0.45759233832359314},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4502512812614441},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.16887140274047852},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06384056806564331}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:11263578","doi":null,"is_oa":false,"landing_page_url":"http://www.osti.gov/scitech/biblio/5373546-effect-cysteine-point-mutation-tryptophan-catalytic-activity-aldehyde-dehydrogenase","pdf_url":null,"source":{"id":"https://openalex.org/S4306510356","display_name":"FASEB Journal (Federation of American Societies for Experimental Biology); (United States)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"FASEB Journal (Federation of American Societies for Experimental Biology); (United States)","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2040683582","https://openalex.org/W1551352684","https://openalex.org/W1023130194","https://openalex.org/W2042156008","https://openalex.org/W94481634","https://openalex.org/W1543846266","https://openalex.org/W176348080","https://openalex.org/W190283025","https://openalex.org/W49648289","https://openalex.org/W2034504157"],"abstract_inverted_index":{"Chemical":[0],"modifications":[1],"of":[2,20,168],"tetrameric":[3],"horse":[4],"liver":[5,27],"aldehyde":[6],"dehydrogenase":[7],"with":[8,25,113,117],"{sup":[9],"14}(C)-N-ethylmaleimide":[10],"(NEM)":[11],"indicated":[12],"that":[13,44,151,166],"Cys-49":[14],"and":[15,83,94,116,138,143],"162":[16],"could":[17],"be":[18],"components":[19],"the":[21,38,69,85,122,136,158,173,180],"active":[22,34,54],"site.":[23],"Studies":[24],"human":[26],"ALDH":[28],"identified":[29],"CYS-302":[30],"as":[31,52,80,88,127],"a":[32,81,169],"possible":[33],"site":[35,55],"residue.":[36],"Mutating":[37],"three":[39,58,72],"residues":[40],"to":[41,62,67,98,125,182],"Ala":[42],"showed":[43,150],"only":[45],"C302A":[46],"was":[47,78,111,153],"inactive,":[48,75],"supporting":[49],"its":[50],"role":[51],"an":[53,65,99,103,114,118,128],"component.":[56],"The":[57,92],"Cys":[59],"were":[60,74,157],"changed":[61],"Trp":[63],"in":[64],"attempt":[66],"mimic":[68],"NEM-effect.":[70],"All":[71],"mutants":[73,160],"though":[76],"each":[77],"expressed":[79],"tetramer":[82],"had":[84],"same":[86],"pI":[87],"did":[89,145],"native":[90,95,139,162],"enzyme.":[91,163],"mutant":[93],"enzymes":[96],"bound":[97],"{alpha}-cyanocinnamate":[100],"affinity":[101,129],"column,":[102],"ALDH-specific":[104],"column":[105,121,130],"described":[106],"by":[107],"others.":[108],"Differential":[109],"binding":[110],"found":[112],"AMP-Sepharose":[115],"EA-Sepharose":[119],"6Bp-hydroxyacetophenone":[120],"authors":[123],"designed":[124],"act":[126],"for":[131,172],"mammalian":[132],"ALDH.":[133],"To":[134],"these":[135],"C49W":[137,152],"bound,":[140],"while":[141],"C162W":[142],"C302W":[144],"not.":[146],"Pulse":[147],"chase":[148],"labeling":[149],"less":[154],"stable":[155],"than":[156],"other":[159],"or":[161],"It":[164],"appears":[165],"substitution":[167],"bulky":[170],"residue":[171],"non":[174],"essential,":[175],"but":[176],"reactive":[177],"cysteines":[178],"caused":[179],"enzyme":[181],"become":[183],"inactive.":[184]},"counts_by_year":[],"updated_date":"2026-10-02T10:15:12.560889","created_date":"2016-06-24T00:00:00"}
