{"id":"https://openalex.org/W112407545","doi":null,"title":"Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains.","display_name":"Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains.","publication_year":1988,"publication_date":"1988-11-01","ids":{"openalex":"https://openalex.org/W112407545","mag":"112407545","pmid":"https://pubmed.ncbi.nlm.nih.gov/3078962"},"language":"en","primary_location":{"id":"pmid:3078962","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3078962","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Oncogene","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5048038268","display_name":"Masahide Takahashi","orcid":"https://orcid.org/0000-0002-2803-2683","observed_orcids":["https://orcid.org/0000-0002-2803-2683"]},"institutions":[{"id":"https://openalex.org/I4405286296","display_name":"Aichi Cancer Center Research Institute","ror":"https://ror.org/01rsenb03","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210133851","https://openalex.org/I4405286296"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"M Takahashi","raw_affiliation_strings":["Laboratory of Experimental Pathology, Aichi Cancer Center Research Institute, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Experimental Pathology, Aichi Cancer Center Research Institute, Japan","institution_ids":["https://openalex.org/I4405286296"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031276926","display_name":"Y Buma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210133851","display_name":"Aichi Cancer Center","ror":"https://ror.org/03kfmm080","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210133851"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Y Buma","raw_affiliation_strings":["Aichi Cancer Center Hospital and Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aichi Cancer Center Hospital and Research Institute","institution_ids":["https://openalex.org/I4210133851"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101442315","display_name":"Takashi Iwamoto","orcid":"https://orcid.org/0000-0003-3644-3121","observed_orcids":["https://orcid.org/0000-0003-3644-3121"]},"institutions":[{"id":"https://openalex.org/I4210133851","display_name":"Aichi Cancer Center","ror":"https://ror.org/03kfmm080","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210133851"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"T Iwamoto","raw_affiliation_strings":["Aichi Cancer Center Hospital and Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aichi Cancer Center Hospital and Research Institute","institution_ids":["https://openalex.org/I4210133851"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018558385","display_name":"Yutaka Inaguma","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210133851","display_name":"Aichi Cancer Center","ror":"https://ror.org/03kfmm080","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210133851"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Y Inaguma","raw_affiliation_strings":["Aichi Cancer Center Hospital and Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aichi Cancer Center Hospital and Research Institute","institution_ids":["https://openalex.org/I4210133851"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011808590","display_name":"Haruna Ikeda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210133851","display_name":"Aichi Cancer Center","ror":"https://ror.org/03kfmm080","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210133851"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"H Ikeda","raw_affiliation_strings":["Aichi Cancer Center Hospital and Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aichi Cancer Center Hospital and Research Institute","institution_ids":["https://openalex.org/I4210133851"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109224906","display_name":"H Hiai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210133851","display_name":"Aichi Cancer Center","ror":"https://ror.org/03kfmm080","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210133851"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"H Hiai","raw_affiliation_strings":["Aichi Cancer Center Hospital and Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aichi Cancer Center Hospital and Research Institute","institution_ids":["https://openalex.org/I4210133851"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.0415,"has_fulltext":false,"cited_by_count":443,"citation_normalized_percentile":{"value":0.9463099,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":91,"max":99},"biblio":{"volume":"3","issue":"5","first_page":"571","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13779","display_name":"Cancer Mechanisms and Therapy","score":0.22390000522136688,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13779","display_name":"Cancer Mechanisms and Therapy","score":0.22390000522136688,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11559","display_name":"Retinoids in leukemia and cellular processes","score":0.22110000252723694,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12159","display_name":"T-cell and Retrovirus Studies","score":0.057100001722574234,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ret-proto-oncogene","display_name":"RET proto-oncogene","score":0.9039999842643738},{"id":"https://openalex.org/keywords/tyrosine-kinase","display_name":"tyrosine kinase","score":0.8370000123977661},{"id":"https://openalex.org/keywords/transmembrane-domain","display_name":"transmembrane domain","score":0.9509999752044678},{"id":"https://openalex.org/keywords/transmembrane-receptors","display_name":"transmembrane receptors","score":0.7649999856948853},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.7210000157356262},{"id":"https://openalex.org/keywords/n-terminal-truncation","display_name":"N-terminal truncation","score":0.7910000085830688},{"id":"https://openalex.org/keywords/c-terminal-truncation","display_name":"C-terminal truncation","score":0.9150000214576721},{"id":"https://openalex.org/keywords/oncogenic-transformation","display_name":"oncogenic transformation","score":0.7390000224113464},{"id":"https://openalex.org/keywords/focus-formation","display_name":"focus formation","score":0.6499999761581421},{"id":"https://openalex.org/keywords/tissue-specific-transcription","display_name":"tissue-specific transcription","score":0.6539999842643738},{"id":"https://openalex.org/keywords/spinal-cord","display_name":"spinal cord","score":0.8830000162124634},{"id":"https://openalex.org/keywords/lymphadenopathy","display_name":"lymphadenopathy","score":0.9319999814033508},{"id":"https://openalex.org/keywords/lpr-lpr-mice","display_name":"lpr/lpr mice","score":0.9670000076293945}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8395704030990601},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.8075765371322632},{"id":"https://openalex.org/C2779644188","wikidata":"https://www.wikidata.org/wiki/Q415976","display_name":"RET proto-oncogene","level":5,"score":0.6833404302597046},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.6440346837043762},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5762767791748047},{"id":"https://openalex.org/C2781018059","wikidata":"https://www.wikidata.org/wiki/Q207609","display_name":"Oncogene","level":4,"score":0.5343412756919861},{"id":"https://openalex.org/C118892022","wikidata":"https://www.wikidata.org/wiki/Q7834587","display_name":"Transmembrane domain","level":3,"score":0.5219408869743347},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5189868807792664},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4975793659687042},{"id":"https://openalex.org/C42362537","wikidata":"https://www.wikidata.org/wiki/Q87099740","display_name":"Tyrosine kinase","level":3,"score":0.47858190536499023},{"id":"https://openalex.org/C101544691","wikidata":"https://www.wikidata.org/wiki/Q420511","display_name":"Receptor tyrosine kinase","level":3,"score":0.4728456139564514},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.4614163339138031},{"id":"https://openalex.org/C2776165026","wikidata":"https://www.wikidata.org/wiki/Q188017","display_name":"Tyrosine","level":2,"score":0.44305628538131714},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.29496827721595764},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.25748181343078613},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.24766868352890015},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16291269659996033},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.14943557977676392},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.12517687678337097},{"id":"https://openalex.org/C13514818","wikidata":"https://www.wikidata.org/wiki/Q5552256","display_name":"Germline mutation","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D000090063","descriptor_name":"Proto-Oncogene Mas","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002462","descriptor_name":"Cell Membrane","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011505","descriptor_name":"Protein-Tyrosine Kinases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011518","descriptor_name":"Proto-Oncogene Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011519","descriptor_name":"Proto-Oncogenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020794","descriptor_name":"Receptor Protein-Tyrosine Kinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D029721","descriptor_name":"Drosophila Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051096","descriptor_name":"Proto-Oncogene Proteins c-ret","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:3078962","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3078962","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Oncogene","raw_type":"Journal Article"},{"id":"mag:112407545","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3078962","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4251082500","https://openalex.org/W79816168","https://openalex.org/W2277460059","https://openalex.org/W1863695472","https://openalex.org/W4248668685","https://openalex.org/W4241998134","https://openalex.org/W2268631203","https://openalex.org/W2027621573","https://openalex.org/W7096418890","https://openalex.org/W2079212189"],"abstract_inverted_index":{"The":[0,109],"nucleotide":[1],"sequence":[2,29,67,144],"of":[3,18,27,65,68,74,93,104,113,129,147,150,156,165,179],"the":[4,66,69,75,84,87,94,105,114,133,142,151,157],"ret":[5,70,76,95,106,115,152,158],"proto-oncogene":[6,71,96,159],"has":[7],"been":[8],"determined":[9],"from":[10,14],"cDNA":[11,16,130],"clones":[12],"isolated":[13],"a":[15,19,34,44,138,163],"library":[17],"THP-1":[20],"human":[21],"monocytic":[22],"leukemia":[23],"cell":[24],"line.":[25],"Analysis":[26],"this":[28],"indicates":[30],"that":[31,73,128],"it":[32,54],"encodes":[33],"protein":[35],"which":[36,59],"is":[37],"structurally":[38],"related":[39],"to":[40,83],"transmembrane":[41,62],"receptors":[42],"with":[43,72,99],"cytoplasmic":[45],"tyrosine":[46],"kinase":[47],"domain.":[48],"Unlike":[49],"most":[50],"growth":[51],"factor":[52],"receptors,":[53],"contains":[55],"two":[56],"hydrophobic":[57],"regions":[58],"are":[60],"potential":[61,149],"domains.":[63],"Comparison":[64],"transforming":[77,107,148],"gene":[78],"revealed":[79],"that,":[80],"in":[81,145,162,172],"addition":[82],"amino-terminal":[85,119,134],"truncation,":[86],"last":[88],"51":[89],"carboxy-terminal":[90,143],"amino":[91,102],"acids":[92,103],"were":[97],"replaced":[98],"9":[100],"unrelated":[101],"gene.":[108],"focus":[110],"forming":[111],"activity":[112],"cDNA,":[116],"containing":[117,131],"both":[118],"and":[120,177,181],"caboxy-terminal":[121],"truncations,":[122],"was":[123,160],"approximately":[124],"13-fold":[125],"higher":[126],"than":[127],"only":[132],"truncation.":[135],"This":[136],"suggests":[137],"possible":[139],"role":[140],"for":[141],"activation":[146],"proto-oncogene.":[153],"When":[154],"transcription":[155,171],"examined":[161],"variety":[164],"mouse":[166,174],"tissues,":[167],"we":[168],"detected":[169],"its":[170],"normal":[173],"spinal":[175],"cord":[176],"lymphadenopathy":[178],"C3H/HeJ-gld/gld":[180],"MRL/Mp-lpr/lpr":[182],"mice.":[183]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":9},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":11},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":7},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}