{"id":"https://openalex.org/W110655149","doi":"https://doi.org/10.1096/fasebj.21.5.a660-c","title":"The DNA methylation inhibitor 5\u2010aza\u20102\u2032\u2010deoxycytidine (5\u2010azadC) induces reversible genome\u2010wide DNA damage that is distinctly influenced by DNA methyltransferases (DNMTs) 1 and 3B","display_name":"The DNA methylation inhibitor 5\u2010aza\u20102\u2032\u2010deoxycytidine (5\u2010azadC) induces reversible genome\u2010wide DNA damage that is distinctly influenced by DNA methyltransferases (DNMTs) 1 and 3B","publication_year":2007,"publication_date":"2007-04-01","ids":{"openalex":"https://openalex.org/W110655149","doi":"https://doi.org/10.1096/fasebj.21.5.a660-c","mag":"110655149"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.21.5.a660-c","doi":"https://doi.org/10.1096/fasebj.21.5.a660-c","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.21.5.a660-c","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5077746203","display_name":"Keith D. Robertson","orcid":"https://orcid.org/0000-0002-7508-3328","observed_orcids":["https://orcid.org/0000-0002-7508-3328"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Keith D Robertson","raw_affiliation_strings":["Biochemistry & Molecular Biology University of Florida Box 100245, 1600 S.W. Archer Rd. Gainesville FL 32610","Biochemistry & Molecular Biology, University of Florida, Box 100245, 1600 S.W. Archer Rd., Gainesville, FL, 32610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry & Molecular Biology University of Florida Box 100245, 1600 S.W. Archer Rd. Gainesville FL 32610","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Biochemistry & Molecular Biology, University of Florida, Box 100245, 1600 S.W. Archer Rd., Gainesville, FL, 32610","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041786738","display_name":"Umesh T. Sankpal","orcid":"https://orcid.org/0000-0001-6696-2315","observed_orcids":["https://orcid.org/0000-0001-6696-2315"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Umesh Sankpal","raw_affiliation_strings":["Biochemistry & Molecular Biology University of Florida Box 100245, 1600 S.W. Archer Rd. Gainesville FL 32610","Biochemistry & Molecular Biology, University of Florida, Box 100245, 1600 S.W. Archer Rd., Gainesville, FL, 32610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry & Molecular Biology University of Florida Box 100245, 1600 S.W. Archer Rd. Gainesville FL 32610","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Biochemistry & Molecular Biology, University of Florida, Box 100245, 1600 S.W. Archer Rd., Gainesville, FL, 32610","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102995801","display_name":"Kevin D. Brown","orcid":"https://orcid.org/0000-0003-3020-8964","observed_orcids":["https://orcid.org/0000-0003-3020-8964"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kevin D Brown","raw_affiliation_strings":["Biochemistry & Molecular Biology University of Florida Box 100245, 1600 S.W. Archer Rd. Gainesville FL 32610","Biochemistry & Molecular Biology, University of Florida, Box 100245, 1600 S.W. Archer Rd., Gainesville, FL, 32610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemistry & Molecular Biology University of Florida Box 100245, 1600 S.W. Archer Rd. Gainesville FL 32610","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"Biochemistry & Molecular Biology, University of Florida, Box 100245, 1600 S.W. Archer Rd., Gainesville, FL, 32610","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I33213144"],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.1323,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.47739447,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"21","issue":"5","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.6996999979019165,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.6996999979019165,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.2827000021934509,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.00139999995008111,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-methylation-inhibitors","display_name":"DNA methylation inhibitors","score":0.8629999756813049},{"id":"https://openalex.org/keywords/dna-damage-response","display_name":"DNA damage response","score":0.800000011920929},{"id":"https://openalex.org/keywords/dnmt1","display_name":"DNMT1","score":0.9399999976158142},{"id":"https://openalex.org/keywords/dnmt3b","display_name":"DNMT3B","score":1.0},{"id":"https://openalex.org/keywords/dna-double-strand-breaks","display_name":"DNA double-strand breaks","score":0.847000002861023},{"id":"https://openalex.org/keywords/atm","display_name":"ATM","score":0.7680000066757202},{"id":"https://openalex.org/keywords/attenuated-total-reflectance","display_name":"ATR","score":0.7400000095367432},{"id":"https://openalex.org/keywords/chk1","display_name":"Chk1","score":0.800000011920929},{"id":"https://openalex.org/keywords/brca1","display_name":"BRCA1","score":0.843999981880188},{"id":"https://openalex.org/keywords/nbs1","display_name":"NBS1","score":0.996999979019165},{"id":"https://openalex.org/keywords/rad51","display_name":"RAD51","score":0.9990000128746033},{"id":"https://openalex.org/keywords/gamma-h2ax","display_name":"\u03b3H2AX","score":0.906000018119812},{"id":"https://openalex.org/keywords/p53-activation","display_name":"p53 activation","score":0.9679999947547913},{"id":"https://openalex.org/keywords/g2-cell-cycle-arrest","display_name":"G2 cell cycle arrest","score":0.8040000200271606},{"id":"https://openalex.org/keywords/dna-repair","display_name":"DNA repair","score":0.6940000057220459},{"id":"https://openalex.org/keywords/dna-methyltransferases","display_name":"DNA methyltransferases","score":0.5239999890327454}],"concepts":[{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.7093455791473389},{"id":"https://openalex.org/C190727270","wikidata":"https://www.wikidata.org/wiki/Q874745","display_name":"DNA methylation","level":4,"score":0.6293122172355652},{"id":"https://openalex.org/C91965660","wikidata":"https://www.wikidata.org/wiki/Q415875","display_name":"Methyltransferase","level":4,"score":0.6167052388191223},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.4739682376384735},{"id":"https://openalex.org/C2777142704","wikidata":"https://www.wikidata.org/wiki/Q15313456","display_name":"DNMT3B","level":5,"score":0.4695846438407898},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4484894871711731},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43282386660575867},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.42995449900627136},{"id":"https://openalex.org/C41091548","wikidata":"https://www.wikidata.org/wiki/Q26939","display_name":"Epigenetics","level":3,"score":0.42487823963165283},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.42274153232574463},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41223111748695374},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.40610527992248535},{"id":"https://openalex.org/C33288867","wikidata":"https://www.wikidata.org/wiki/Q518328","display_name":"Methylation","level":3,"score":0.3067523241043091},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16254210472106934},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11612489819526672},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.07556605339050293}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.21.5.a660-c","doi":"https://doi.org/10.1096/fasebj.21.5.a660-c","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.21.5.a660-c","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9537000060081482,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2754435990","display_name":"De Novo Methyltransferase Function in Chromatin and Cancer","funder_award_id":"R01CA114229","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G4642975718","display_name":"MECHANISMS OF DE NOVO METHYLATION IN CANCER","funder_award_id":"K22CA084535","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2040393829","https://openalex.org/W2024088186","https://openalex.org/W1982393817","https://openalex.org/W2081694922","https://openalex.org/W2041278400","https://openalex.org/W2040882330","https://openalex.org/W2076437565","https://openalex.org/W4307230346","https://openalex.org/W2071285347","https://openalex.org/W2090094306"],"abstract_inverted_index":{"Genome\u2010wide":[0],"DNA":[1,17,50,57,94,103],"methylation":[2,18],"patterns":[3],"are":[4],"frequently":[5],"deregulated":[6],"in":[7,14,20,72,80,107,151],"cancer":[8],"therefore":[9],"there":[10],"is":[11],"significant":[12],"interest":[13],"targeting":[15],"the":[16,56,66,176,181],"machinery":[19],"tumor":[21,61],"cells":[22,62,147,167],"using":[23],"nucleoside":[24],"analogs":[25],"of":[26,40,60,68,82,92,102,125,157,180,184,186],"cytosine,":[27],"such":[28],"as":[29],"5\u2010azadC.":[30],"5\u2010azadC":[31,64,77,97,187],"exerts":[32],"its":[33],"anti\u2010tumor":[34],"effects":[35],"by":[36,139,202],"two":[37],"mechanisms;":[38],"reactivation":[39],"aberrantly":[41],"hypermethylated":[42],"growth":[43,85],"regulatory":[44],"genes":[45],"and":[46,65,70,87,111,123,137,142,160,206],"cytoxicity":[47],"resulting":[48],"from":[49],"damage.":[51],"Objective.":[52],"To":[53],"better":[54],"characterize":[55],"damage":[58,95,113],"response":[59],"to":[63,100,191,195],"role":[67],"DNMT1":[69,145],"DNMT3B":[71,165],"modulating":[73],"this":[74,112,196],"process.":[75],"Results.":[76],"treatment":[78,98],"results":[79],"depletion":[81],"soluble":[83],"DNMTs,":[84],"inhibition,":[86],"G2":[88],"arrest":[89],"\u2010":[90],"hallmarks":[91],"a":[93],"response.":[96],"led":[99],"formation":[101],"double":[104],"strand":[105,127],"breaks":[106],"an":[108],"ATM\u2010dependent":[109],"manner":[110],"was":[114,200],"repaired":[115],"following":[116],"drug":[117],"removal.":[118],"Further":[119],"analysis":[120,179],"revealed":[121],"activation":[122],"relocalization":[124],"key":[126],"break":[128],"repair":[129],"proteins":[130],"including":[131,154],"ATM,":[132],"ATR,":[133],"CHK1,":[134],"BRCA1,":[135],"NBS1,":[136],"RAD51":[138],"western":[140],"blotting":[141],"immunofluorescence.":[143],"Significantly,":[144],"deficient":[146,166],"demonstrated":[148],"profound":[149],"defects":[150],"these":[152],"responses,":[153],"complete":[155],"lack":[156],"gammaH2AX":[158],"induction":[159],"blunted":[161],"p53":[162],"activation,":[163],"while":[164],"generally":[168],"showed":[169],"milder":[170],"defects.":[171],"Conclusions.":[172],"This":[173,198],"study":[174],"provides":[175],"first":[177],"detailed":[178],"cytotoxic":[182],"mechanism":[183],"action":[185],"which":[188],"may":[189],"help":[190],"optimize":[192],"patient":[193],"responses":[194],"agent.":[197],"work":[199],"supported":[201],"NIH":[203],"grants":[204],"R01CA114229":[205],"K22CA084535":[207],"(KDR).":[208]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}