{"id":"https://openalex.org/W11041446","doi":"https://doi.org/10.31274/rtd-180813-8707","title":"Purification, surfactant stabilization, molecular weight studies, and divalent metal ion kinetics of dextransucrase from Leuconostoc mesenteroides NRRL B-512F","display_name":"Purification, surfactant stabilization, molecular weight studies, and divalent metal ion kinetics of dextransucrase from Leuconostoc mesenteroides NRRL B-512F","publication_year":1985,"publication_date":"1985-01-01","ids":{"openalex":"https://openalex.org/W11041446","doi":"https://doi.org/10.31274/rtd-180813-8707","mag":"11041446"},"language":"en","primary_location":{"id":"doi:10.31274/rtd-180813-8707","doi":"https://doi.org/10.31274/rtd-180813-8707","is_oa":false,"landing_page_url":"https://doi.org/10.31274/rtd-180813-8707","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Iowa State University, Digital Repository","raw_type":"dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000482958","display_name":"Arthur W. Miller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Arthur West Miller","raw_affiliation_strings":["Iowa State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Iowa State University","institution_ids":["https://openalex.org/I173911158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5000482958"],"corresponding_institution_ids":["https://openalex.org/I173911158"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9243000149726868,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9243000149726868,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12192","display_name":"Polysaccharides Composition and Applications","score":0.02500000037252903,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.02019999921321869,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dextransucrase","display_name":"dextransucrase","score":0.9879999756813049},{"id":"https://openalex.org/keywords/leuconostoc-mesenteroides","display_name":"Leuconostoc mesenteroides","score":0.9679999947547913},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.796999990940094},{"id":"https://openalex.org/keywords/surfactant-stabilization","display_name":"surfactant stabilization","score":0.8659999966621399},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.4519999921321869},{"id":"https://openalex.org/keywords/proteolytic-processing","display_name":"proteolytic processing","score":0.652999997138977},{"id":"https://openalex.org/keywords/divalent-metal-ions","display_name":"divalent metal ions","score":0.9020000100135803},{"id":"https://openalex.org/keywords/zinc","display_name":"zinc","score":0.5270000100135803},{"id":"https://openalex.org/keywords/calcium-activation","display_name":"calcium activation","score":0.7289999723434448},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5040000081062317},{"id":"https://openalex.org/keywords/dextran-synthesis","display_name":"dextran synthesis","score":0.7059999704360962},{"id":"https://openalex.org/keywords/glucan-biosynthesis","display_name":"glucan biosynthesis","score":0.453000009059906},{"id":"https://openalex.org/keywords/nonionic-detergents","display_name":"nonionic detergents","score":0.6299999952316284},{"id":"https://openalex.org/keywords/ionic-detergents","display_name":"ionic detergents","score":0.6140000224113464}],"concepts":[{"id":"https://openalex.org/C2777957631","wikidata":"https://www.wikidata.org/wiki/Q146954","display_name":"Leuconostoc mesenteroides","level":4,"score":0.9647836685180664},{"id":"https://openalex.org/C2777423746","wikidata":"https://www.wikidata.org/wiki/Q3706357","display_name":"Dextransucrase","level":5,"score":0.9088602066040039},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6761491298675537},{"id":"https://openalex.org/C74884574","wikidata":"https://www.wikidata.org/wiki/Q171407","display_name":"Divalent","level":2,"score":0.675743043422699},{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.6430429816246033},{"id":"https://openalex.org/C58226133","wikidata":"https://www.wikidata.org/wiki/Q1324199","display_name":"Pulmonary surfactant","level":2,"score":0.5810263752937317},{"id":"https://openalex.org/C544153396","wikidata":"https://www.wikidata.org/wiki/Q11426","display_name":"Metal","level":2,"score":0.45243316888809204},{"id":"https://openalex.org/C199164860","wikidata":"https://www.wikidata.org/wiki/Q6822745","display_name":"Metal ions in aqueous solution","level":3,"score":0.43604713678359985},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3707636594772339},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.13179156184196472},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.12195929884910583},{"id":"https://openalex.org/C2775920511","wikidata":"https://www.wikidata.org/wiki/Q161249","display_name":"Lactic acid","level":3,"score":0.10290446877479553},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.08317852020263672},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.31274/rtd-180813-8707","doi":"https://doi.org/10.31274/rtd-180813-8707","is_oa":false,"landing_page_url":"https://doi.org/10.31274/rtd-180813-8707","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Iowa State University, Digital Repository","raw_type":"dissertation"},{"id":"pmh:oai:lib.dr.iastate.edu:rtd-9728","doi":null,"is_oa":false,"landing_page_url":"https://lib.dr.iastate.edu/rtd/8729","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Retrospective Theses and Dissertations","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2077362344","https://openalex.org/W316915609","https://openalex.org/W2013404491","https://openalex.org/W2006399828","https://openalex.org/W1969622078","https://openalex.org/W1995054117","https://openalex.org/W2005650303","https://openalex.org/W2084379420","https://openalex.org/W4234385611","https://openalex.org/W2376176905"],"abstract_inverted_index":{"Dextransucrase":[0],"from":[1,16,257],"Leuconostoc":[2],"mesenteroides":[3],"NRRL":[4],"B-512F,":[5],"an":[6,10,107,157,191],"enzyme":[7,70,83],"that":[8,125,231],"synthesizes":[9],"(alpha)-(1(--->)6)-linked":[11],"glucan":[12],"with":[13,164,181],"(alpha)-(1(--->)3)-linked":[14],"branches":[15],"the":[17,36,131,244,258,266,272],"glucosyl":[18],"moiety":[19],"of":[20,38,54,75,85,88,134,145,153,246,268],"sucrose,":[21],"was":[22,49,95,123,156,187,190,204,210],"stabilized":[23],"against":[24,28],"thermal":[25],"inactivation":[26,29,116],"and":[27,33,64,79,98,117,147,171,199],"caused":[30],"by":[31,35,51,106,130,141,252,265],"dilution":[32],"manipulation":[34],"addition":[37],"nonionic":[39],"detergents,":[40,43],"mild":[41],"ionic":[42],"poly(ethylene":[44],"glycol),":[45],"or":[46,136,262],"high-molecular-weight":[47],"dextran;Dextransucrase":[48],"purified":[50,69],"a":[52,72,103,165,182,205],"sequence":[53],"dextranase":[55],"treatment,":[56],"DEAE-cellulose":[57],"chromatography,":[58],"affinity":[59,167,184],"chromatography":[60],"on":[61,225,271],"Sephadex":[62],"G-200,":[63],"DEAE-Trisacryl":[65],"M":[66],"chromatography.":[67],"The":[68],"had":[71],"specific":[73],"activity":[74,224],"170":[76],"U/mg":[77],"protein":[78,256],"no":[80],"detectable":[81],"carbohydrate;The":[82],"consisted":[84],"two":[86,135],"forms":[87],"differing":[89],"molecular":[90],"weight.":[91],"A":[92,176],"177-kDa":[93],"form":[94,105],"biosynthesized":[96],"initially":[97],"converted":[99],"during":[100,243],"aging":[101],"to":[102,221],"158-kDa":[104],"unknown":[108],"but":[109,140],"probably":[110],"proteolytic":[111],"mechanism.":[112],"By":[113],"using":[114],"radiation":[115],"SDS":[118],"polyacrylamide":[119,226],"gel":[120],"electrophoresis,":[121],"it":[122,209],"determined":[124],"dextran":[126,214],"is":[127],"not":[128,211],"synthesized":[129],"cooperative":[132],"action":[133,267],"more":[137],"peptides":[138,144],"(subunits),":[139],"single":[142],"dextransucrase":[143,155],"177":[146],"158":[148],"kDa;The":[149],"sucrose":[150],"binding":[151],"site":[152,162,180],"B-512F":[154],"inhibitory":[158],"divalent":[159],"metal":[160,178],"ion":[161,179],"also,":[163],"higher":[166],"for":[168,174,185,213],"Zn('2+),":[169,197],"Ni('2+),":[170,198],"Co('2+)":[172,200],"than":[173],"Ca('2+).":[175],"second":[177],"high":[183],"Ca('2+)":[186,189,203],"found.":[188],"activator":[192],"at":[193],"this":[194],"site,":[195],"whereas":[196],"were":[201,232],"inhibitors.":[202],"nonessential":[206],"activator;":[207],"i.e.,":[208],"required":[212],"synthesis;The":[215],"periodic":[216],"acid-Schiff":[217],"stain,":[218],"when":[219],"used":[220],"detect":[222],"glycansucrase":[223],"gels,":[227,248],"stained":[228,247,273],"many":[229],"proteins":[230],"neither":[233],"glycoproteins":[234],"nor":[235],"active":[236],"glycansucrases.":[237],"This":[238],"artifactual":[239],"staining,":[240,261],"which":[241],"increased":[242],"storage":[245],"could":[249],"be":[250],"prevented":[251],"proteolytically":[253],"removing":[254],"all":[255],"gels":[259],"before":[260],"considerably":[263],"slowed":[264],"crosslinking":[269],"agents":[270],"gels.":[274]},"counts_by_year":[{"year":2020,"cited_by_count":1}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2016-06-24T00:00:00"}