{"id":"https://openalex.org/W106265182","doi":null,"title":"A functional analysis of the epigenetic regulator CTCF","display_name":"A functional analysis of the epigenetic regulator CTCF","publication_year":2010,"publication_date":"2010-08-20","ids":{"openalex":"https://openalex.org/W106265182","mag":"106265182"},"language":"en","primary_location":{"id":"pmh:oai:www.ideals.illinois.edu:2142/16867","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2142/16867","pdf_url":"http://hdl.handle.net/2142/16867","source":{"id":"https://openalex.org/S4306401358","display_name":"Illinois Digital Environment for Access to Learning and Scholarship (University of Illinois at Urbana-Champaign)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801919071","host_organization_name":"University of Illinois System","host_organization_lineage":["https://openalex.org/I2801919071"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/2142/16867","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076672455","display_name":"Neal Stanton Kitchen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Neal Stanton Kitchen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5076672455"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00898924,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ctcf","display_name":"CTCF","score":0.996999979019165},{"id":"https://openalex.org/keywords/chromatin-decondensation","display_name":"chromatin decondensation","score":0.8880000114440918},{"id":"https://openalex.org/keywords/transactivation-domain","display_name":"transactivation domain","score":0.8830000162124634},{"id":"https://openalex.org/keywords/at-hook-motif","display_name":"AT-hook motif","score":0.8989999890327454},{"id":"https://openalex.org/keywords/chd8","display_name":"CHD8","score":0.9660000205039978},{"id":"https://openalex.org/keywords/chromatin-remodeling","display_name":"chromatin remodeling","score":0.8339999914169312},{"id":"https://openalex.org/keywords/sumoylation","display_name":"SUMOylation","score":0.7260000109672546},{"id":"https://openalex.org/keywords/enhancer-blocking","display_name":"enhancer blocking","score":0.8610000014305115},{"id":"https://openalex.org/keywords/chromatin-looping","display_name":"chromatin looping","score":0.5820000171661377},{"id":"https://openalex.org/keywords/nuclear-organization","display_name":"nuclear organization","score":0.5070000290870667},{"id":"https://openalex.org/keywords/transcriptional-repression","display_name":"transcriptional repression","score":0.5210000276565552},{"id":"https://openalex.org/keywords/histone-acetyltransferase","display_name":"histone acetyltransferase","score":0.6779999732971191},{"id":"https://openalex.org/keywords/cofactor-recruitment","display_name":"cofactor recruitment","score":0.6169999837875366},{"id":"https://openalex.org/keywords/dna-loop-formation","display_name":"DNA loop formation","score":0.3070000112056732}],"concepts":[{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.7370420694351196},{"id":"https://openalex.org/C2778760011","wikidata":"https://www.wikidata.org/wiki/Q21163346","display_name":"CTCF","level":5,"score":0.5680414438247681},{"id":"https://openalex.org/C41091548","wikidata":"https://www.wikidata.org/wiki/Q26939","display_name":"Epigenetics","level":3,"score":0.47778424620628357},{"id":"https://openalex.org/C55112680","wikidata":"https://www.wikidata.org/wiki/Q190549","display_name":"Functional analysis","level":3,"score":0.42116329073905945},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.41026541590690613},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32494062185287476},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3246666193008423},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.23729208111763},{"id":"https://openalex.org/C111936080","wikidata":"https://www.wikidata.org/wiki/Q913367","display_name":"Enhancer","level":4,"score":0.1174282431602478},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.11098471283912659},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08728283643722534}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:www.ideals.illinois.edu:2142/16867","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2142/16867","pdf_url":"http://hdl.handle.net/2142/16867","source":{"id":"https://openalex.org/S4306401358","display_name":"Illinois Digital Environment for Access to Learning and Scholarship (University of Illinois at Urbana-Champaign)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801919071","host_organization_name":"University of Illinois System","host_organization_lineage":["https://openalex.org/I2801919071"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"mag:106265182","doi":null,"is_oa":false,"landing_page_url":"https://www.ideals.illinois.edu/handle/2142/16867","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:www.ideals.illinois.edu:2142/16867","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2142/16867","pdf_url":"http://hdl.handle.net/2142/16867","source":{"id":"https://openalex.org/S4306401358","display_name":"Illinois Digital Environment for Access to Learning and Scholarship (University of Illinois at Urbana-Champaign)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801919071","host_organization_name":"University of Illinois System","host_organization_lineage":["https://openalex.org/I2801919071"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W106265182.pdf","grobid_xml":"https://content.openalex.org/works/W106265182.grobid-xml"},"referenced_works_count":198,"referenced_works":["https://openalex.org/W41831396","https://openalex.org/W204316109","https://openalex.org/W1506635079","https://openalex.org/W1557080454","https://openalex.org/W1558013374","https://openalex.org/W1643500256","https://openalex.org/W1669374546","https://openalex.org/W1840859280","https://openalex.org/W1893658886","https://openalex.org/W1927984809","https://openalex.org/W1969413206","https://openalex.org/W1970321798","https://openalex.org/W1970385007","https://openalex.org/W1970844439","https://openalex.org/W1971358793","https://openalex.org/W1971615450","https://openalex.org/W1971910773","https://openalex.org/W1971918585","https://openalex.org/W1973075378","https://openalex.org/W1973342161","https://openalex.org/W1973726475","https://openalex.org/W1974168680","https://openalex.org/W1975403665","https://openalex.org/W1975680353","https://openalex.org/W1976365653","https://openalex.org/W1976622956","https://openalex.org/W1978693761","https://openalex.org/W1979591315","https://openalex.org/W1979965243","https://openalex.org/W1980051146","https://openalex.org/W1980197198","https://openalex.org/W1981437154","https://openalex.org/W1986675604","https://openalex.org/W1988294855","https://openalex.org/W1989373989","https://openalex.org/W1989855790","https://openalex.org/W1990064913","https://openalex.org/W1991457889","https://openalex.org/W1992574814","https://openalex.org/W1995562162","https://openalex.org/W1996183597","https://openalex.org/W1998271864","https://openalex.org/W2002644881","https://openalex.org/W2002877822","https://openalex.org/W2006061308","https://openalex.org/W2008420943","https://openalex.org/W2008722912","https://openalex.org/W2009714281","https://openalex.org/W2011457803","https://openalex.org/W2015416439","https://openalex.org/W2016136969","https://openalex.org/W2016252630","https://openalex.org/W2018201204","https://openalex.org/W2018413423","https://openalex.org/W2019937806","https://openalex.org/W2021080331","https://openalex.org/W2021798161","https://openalex.org/W2022110934","https://openalex.org/W2022685983","https://openalex.org/W2024634513","https://openalex.org/W2026180423","https://openalex.org/W2027781138","https://openalex.org/W2027878791","https://openalex.org/W2027887262","https://openalex.org/W2028022357","https://openalex.org/W2029722028","https://openalex.org/W2030877902","https://openalex.org/W2032167736","https://openalex.org/W2033107200","https://openalex.org/W2036636377","https://openalex.org/W2038774969","https://openalex.org/W2038883903","https://openalex.org/W2039367790","https://openalex.org/W2039395294","https://openalex.org/W2042142148","https://openalex.org/W2043846695","https://openalex.org/W2044000724","https://openalex.org/W2044937161","https://openalex.org/W2045622916","https://openalex.org/W2047005748","https://openalex.org/W2047054611","https://openalex.org/W2049050973","https://openalex.org/W2050005646","https://openalex.org/W2050360708","https://openalex.org/W2054750734","https://openalex.org/W2056296053","https://openalex.org/W2057597572","https://openalex.org/W2058151080","https://openalex.org/W2058528214","https://openalex.org/W2058912883","https://openalex.org/W2060371769","https://openalex.org/W2064038671","https://openalex.org/W2064520673","https://openalex.org/W2065781246","https://openalex.org/W2065794410","https://openalex.org/W2066208998","https://openalex.org/W2067991344","https://openalex.org/W2069172573","https://openalex.org/W2069217355","https://openalex.org/W2069328568","https://openalex.org/W2070182263","https://openalex.org/W2071051659","https://openalex.org/W2074104813","https://openalex.org/W2074906225","https://openalex.org/W2077011077","https://openalex.org/W2077154575","https://openalex.org/W2077543502","https://openalex.org/W2077937279","https://openalex.org/W2078519341","https://openalex.org/W2078526277","https://openalex.org/W2080886565","https://openalex.org/W2082349351","https://openalex.org/W2083278169","https://openalex.org/W2083414728","https://openalex.org/W2085090468","https://openalex.org/W2085509761","https://openalex.org/W2086038224","https://openalex.org/W2086201543","https://openalex.org/W2087571144","https://openalex.org/W2088661602","https://openalex.org/W2088727054","https://openalex.org/W2090632681","https://openalex.org/W2092574488","https://openalex.org/W2093032186","https://openalex.org/W2094601780","https://openalex.org/W2094889529","https://openalex.org/W2096594220","https://openalex.org/W2099104943","https://openalex.org/W2099450122","https://openalex.org/W2104865962","https://openalex.org/W2105427468","https://openalex.org/W2108132333","https://openalex.org/W2108419006","https://openalex.org/W2108741433","https://openalex.org/W2110251865","https://openalex.org/W2112514675","https://openalex.org/W2112642897","https://openalex.org/W2114151755","https://openalex.org/W2115050132","https://openalex.org/W2115125191","https://openalex.org/W2115908781","https://openalex.org/W2116271157","https://openalex.org/W2116622696","https://openalex.org/W2119096223","https://openalex.org/W2119200248","https://openalex.org/W2120330695","https://openalex.org/W2120422553","https://openalex.org/W2120699715","https://openalex.org/W2121361592","https://openalex.org/W2121935539","https://openalex.org/W2122029913","https://openalex.org/W2127497792","https://openalex.org/W2127988376","https://openalex.org/W2128963466","https://openalex.org/W2129795133","https://openalex.org/W2133332261","https://openalex.org/W2133644878","https://openalex.org/W2134087142","https://openalex.org/W2135233174","https://openalex.org/W2136583745","https://openalex.org/W2136932227","https://openalex.org/W2138165389","https://openalex.org/W2138952587","https://openalex.org/W2139519979","https://openalex.org/W2139564227","https://openalex.org/W2140209580","https://openalex.org/W2141567062","https://openalex.org/W2141853045","https://openalex.org/W2143354645","https://openalex.org/W2143716699","https://openalex.org/W2143839770","https://openalex.org/W2145380525","https://openalex.org/W2147600078","https://openalex.org/W2147656349","https://openalex.org/W2148939895","https://openalex.org/W2149936700","https://openalex.org/W2151429210","https://openalex.org/W2151779515","https://openalex.org/W2152968621","https://openalex.org/W2153665057","https://openalex.org/W2155792198","https://openalex.org/W2157031657","https://openalex.org/W2157131586","https://openalex.org/W2157214001","https://openalex.org/W2157835438","https://openalex.org/W2158130734","https://openalex.org/W2159284172","https://openalex.org/W2159827301","https://openalex.org/W2161559093","https://openalex.org/W2161989354","https://openalex.org/W2162972157","https://openalex.org/W2165425175","https://openalex.org/W2166129344","https://openalex.org/W2169993152","https://openalex.org/W2171125622","https://openalex.org/W2172174991","https://openalex.org/W2278834190","https://openalex.org/W2415071912"],"related_works":["https://openalex.org/W7207212913","https://openalex.org/W2315472484","https://openalex.org/W2078526277","https://openalex.org/W2107032654","https://openalex.org/W2010297690","https://openalex.org/W2725640633","https://openalex.org/W2042142148","https://openalex.org/W2549283096","https://openalex.org/W4292590360","https://openalex.org/W1506343731"],"abstract_inverted_index":{"The":[0,168,199],"highly":[1],"conserved":[2,219],"protein":[3,64,265,321],"CTCF":[4,34,57,228,264,295,344,417,452,461],"(CCCTC-binding":[5],"factor)":[6],"participates":[7],"in":[8,35,41,78,153,253,343,375,439,451],"many":[9],"epigenetic":[10],"regulatory":[11,449],"functions,":[12],"including":[13],"insulation,":[14],"imprinting,":[15],"X":[16],"chromosome":[17],"inactivation,":[18],"and":[19,23,40,69,127,139,160,195,371,378,391,418,453],"both":[20],"transcriptional":[21,101,150,448],"activation":[22,151,306],"repression.":[24],"Recent":[25],"data":[26,381],"has":[27,171],"also":[28,170,274,314],"suggested":[29],"a":[30,53,74,82,86,105,149,268,420,430,436],"prominent":[31],"role":[32,438],"for":[33,66,76,256,283,416,422],"mediating":[36],"interactions":[37],"between":[38],"chromosomes":[39],"the":[42,46,60,63,125,131,137,143,185,193,214,218,224,245,248,280,289,297,301,310,319,329,334,340,356,360,376,386,398,407,446,456],"global":[43],"organization":[44],"of":[45,56,62,124,136,142,213,227,247,262,333,351,385,397,445],"nucleus.":[47],"In":[48,145,236],"this":[49,113,272,367,424],"work,":[50],"we":[51,80,115,128,147,242,338,369],"initiated":[52],"functional":[54,326],"analysis":[55],"to":[58,104,134,165,209,223,238,267,278,318,328,348,359,410,426],"determine":[59],"regions":[61,141],"necessary":[65],"regulating":[67],"transcription":[68],"altering":[70,440],"chromatin":[71,97,161,240,352,392,441],"structure.":[72],"As":[73],"reporter":[75],"changes":[77],"chromatin,":[79],"employed":[81],"cell":[83],"line":[84],"bearing":[85],"lac":[87,107],"operator":[88],"array":[89,126,215,249,330,361],"that":[90,117,157,207,288,304,345,383],"can":[91,308],"dramatically":[92],"unfold":[93],"its":[94,325,389],"normally":[95],"condensed":[96],"when":[98],"targeted":[99,269],"by":[100,184,259,294,323,354,362],"proteins":[102,163],"fused":[103],"GFP-tagged":[106],"repressor":[108,119],"DNA":[109,230],"binding":[110],"domain.":[111,364],"With":[112],"system,":[114],"demonstrated":[116,244,401,435],"CTCF-lac":[118],"fusions":[120],"induced":[121],"substantial":[122],"decondensation":[123,350],"have":[129,243,434],"mapped":[130,148],"opening":[132,173,393],"activity":[133,226,425],"portions":[135,212],"N-":[138],"C-terminal":[140,169],"protein.":[144],"addition,":[146],"domain":[152,307,415],"CTCF\u201fs":[154,316],"NT-region":[155],"(NTAD)":[156],"recruits":[158],"HATs":[159],"remodeling":[162,320],"similar":[164],"acidic":[166],"activators.":[167],"two":[172],"domains,":[174],"but":[175],"they":[176],"do":[177],"not":[178],"activate":[179],"transcription.":[180],"Moreover,":[181],"arrays":[182],"opened":[183],"CT":[186,399],"region":[187,400],"adopt":[188],"structures":[189,202],"morphologically":[190],"distinct":[191],"from":[192],"NTAD":[194,387],"other":[196],"activator":[197],"proteins.":[198],"unique":[200],"CT-induced":[201],"require":[203],"an":[204,252,276,305,412],"AT-hook":[205,220],"motif":[206],"appears":[208],"bring":[210],"disparate":[211],"together.":[216],"Thus,":[217],"may":[221],"contribute":[222],"tethering":[225],"during":[229],"loop":[231],"formation":[232],"at":[233],"endogenous":[234],"loci.":[235],"addition":[237],"assessing":[239],"opening,":[241],"utility":[246],"system":[250,273,368],"as":[251],"vivo":[254],"method":[255],"cofactor":[257],"recruitment":[258,281,327],"confirming":[260],"co-localization":[261],"known":[263],"partners":[266],"array.":[270],"Therefore,":[271],"provided":[275],"assay":[277],"identify":[279],"domains":[282,342,450],"each":[284,363],"partner.":[285],"We":[286,313,433],"revealed":[287],"cohesin":[290,311],"complex":[291],"is":[292,406],"recruited":[293,358],"through":[296,331],"NTAD,":[298],"which":[299],"provides":[300],"first":[302,408],"evidence":[303],"recruit":[309],"complex.":[312],"clarified":[315],"interaction":[317],"CHD8":[322],"showing":[324],"one":[332],"CT-opening":[335],"domains.":[336],"Additionally,":[337],"differentiated":[339],"three":[341],"are":[346],"sufficient":[347],"initiate":[349],"structure":[353],"ascertaining":[355],"co-factors":[357,457],"Finally,":[365],"with":[366,460],"found":[370],"analyzed":[372],"SUMO-motif":[373],"sites":[374],"NT-":[377],"CT-regions.":[379],"Our":[380],"suggests":[382,419],"sumoylation":[384,396],"reduces":[388],"transactivation":[390],"activities,":[394],"while":[395],"enhancer":[402,413],"blocking":[403,414],"activity.":[404],"This":[405],"study":[409],"elucidate":[411],"mechanism":[421],"directing":[423],"specific":[427],"loci":[428],"via":[429],"post-translational":[431],"SUMO-modification.":[432],"direct":[437],"structure,":[442],"elucidated":[443],"several":[444],"diverse":[447],"further":[454],"characterized":[455],"potentially":[458],"involved":[459],"function.":[462]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}
