{"id":"https://openalex.org/W104696704","doi":null,"title":"Human Equilibrative Nucleoside Transporter Subtype 1: Structure-Function Analysis Using Cysteine Mutagenesis and Thiol Modifying Techniques","display_name":"Human Equilibrative Nucleoside Transporter Subtype 1: Structure-Function Analysis Using Cysteine Mutagenesis and Thiol Modifying Techniques","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W104696704","mag":"104696704"},"language":"en","primary_location":{"id":"pmh:oai:ir.lib.uwo.ca:etd-1969","doi":null,"is_oa":true,"landing_page_url":"https://ir.lib.uwo.ca/etd/758","pdf_url":"https://ir.lib.uwo.ca/etd/758","source":{"id":"https://openalex.org/S4306400648","display_name":"Scholarship@Western (Western University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I125749732","host_organization_name":"Western University","host_organization_lineage":["https://openalex.org/I125749732"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Thesis and Dissertation Repository","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://ir.lib.uwo.ca/etd/758","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5034997823","display_name":"Jamie Park","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Jamie Park","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5034997823"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00595002,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11193","display_name":"Adenosine and Purinergic Signaling","score":0.6708999872207642,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11193","display_name":"Adenosine and Purinergic Signaling","score":0.6708999872207642,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.26100000739097595,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.011599999852478504,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ent1","display_name":"equilibrative nucleoside transporter 1","score":0.9559999704360962},{"id":"https://openalex.org/keywords/hent1","display_name":"hENT1","score":0.9120000004768372},{"id":"https://openalex.org/keywords/cysteine-residues","display_name":"cysteine residues","score":0.8429999947547913},{"id":"https://openalex.org/keywords/thiol-modification","display_name":"thiol modification","score":0.8209999799728394},{"id":"https://openalex.org/keywords/mtset","display_name":"MTSET","score":0.9879999756813049},{"id":"https://openalex.org/keywords/mtses","display_name":"MTSES","score":0.890999972820282},{"id":"https://openalex.org/keywords/nucleoside-transport","display_name":"nucleoside transport","score":0.6520000100135803},{"id":"https://openalex.org/keywords/transporter-conformation","display_name":"transporter conformation","score":0.5260000228881836}],"concepts":[{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.6507423520088196},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5741606950759888},{"id":"https://openalex.org/C2776543447","wikidata":"https://www.wikidata.org/wiki/Q28734","display_name":"Nucleoside","level":2,"score":0.5566516518592834},{"id":"https://openalex.org/C2780549722","wikidata":"https://www.wikidata.org/wiki/Q7068260","display_name":"Nucleoside transporter","level":4,"score":0.5496467351913452},{"id":"https://openalex.org/C2781324293","wikidata":"https://www.wikidata.org/wiki/Q220410","display_name":"Thiol","level":2,"score":0.48536407947540283},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.47467684745788574},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4673633575439453},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.41730767488479614},{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.3887671232223511},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.35034674406051636},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3337879776954651},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.303666889667511},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.27270591259002686},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22279807925224304},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.09797307848930359}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:ir.lib.uwo.ca:etd-1969","doi":null,"is_oa":true,"landing_page_url":"https://ir.lib.uwo.ca/etd/758","pdf_url":"https://ir.lib.uwo.ca/etd/758","source":{"id":"https://openalex.org/S4306400648","display_name":"Scholarship@Western (Western University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I125749732","host_organization_name":"Western University","host_organization_lineage":["https://openalex.org/I125749732"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Thesis and Dissertation Repository","raw_type":"text"},{"id":"pmh:oai:uwo.scholaris.ca:20.500.14721/30793","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/20.500.14721/30793","pdf_url":null,"source":{"id":"https://openalex.org/S4306400648","display_name":"Scholarship@Western (Western University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I125749732","host_organization_name":"Western University","host_organization_lineage":["https://openalex.org/I125749732"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"thesis"},{"id":"mag:104696704","doi":null,"is_oa":false,"landing_page_url":"http://ir.lib.uwo.ca/cgi/viewcontent.cgi?article=1969&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:ir.lib.uwo.ca:etd-1969","doi":null,"is_oa":true,"landing_page_url":"https://ir.lib.uwo.ca/etd/758","pdf_url":"https://ir.lib.uwo.ca/etd/758","source":{"id":"https://openalex.org/S4306400648","display_name":"Scholarship@Western (Western University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I125749732","host_organization_name":"Western University","host_organization_lineage":["https://openalex.org/I125749732"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Thesis and Dissertation Repository","raw_type":"text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W104696704.pdf","grobid_xml":"https://content.openalex.org/works/W104696704.grobid-xml"},"referenced_works_count":213,"referenced_works":["https://openalex.org/W13247121","https://openalex.org/W102249166","https://openalex.org/W113740469","https://openalex.org/W115576981","https://openalex.org/W251703332","https://openalex.org/W643674881","https://openalex.org/W1493460858","https://openalex.org/W1501769303","https://openalex.org/W1514209753","https://openalex.org/W1522705662","https://openalex.org/W1530387751","https://openalex.org/W1567300212","https://openalex.org/W1587112517","https://openalex.org/W1592442520","https://openalex.org/W1751811227","https://openalex.org/W1764262042","https://openalex.org/W1820454947","https://openalex.org/W1869404997","https://openalex.org/W1871721730","https://openalex.org/W1904630468","https://openalex.org/W1916479001","https://openalex.org/W1916592113","https://openalex.org/W1944373955","https://openalex.org/W1954029123","https://openalex.org/W1963570264","https://openalex.org/W1964286235","https://openalex.org/W1965539285","https://openalex.org/W1965880766","https://openalex.org/W1966753073","https://openalex.org/W1967675162","https://openalex.org/W1968242936","https://openalex.org/W1969125109","https://openalex.org/W1971235587","https://openalex.org/W1971624191","https://openalex.org/W1972660450","https://openalex.org/W1973985383","https://openalex.org/W1974935655","https://openalex.org/W1975921630","https://openalex.org/W1976365732","https://openalex.org/W1981040381","https://openalex.org/W1982303634","https://openalex.org/W1983266664","https://openalex.org/W1985420286","https://openalex.org/W1986952772","https://openalex.org/W1988219529","https://openalex.org/W1990291419","https://openalex.org/W1991022265","https://openalex.org/W1991871309","https://openalex.org/W1992575516","https://openalex.org/W1993761825","https://openalex.org/W1994373739","https://openalex.org/W1994599651","https://openalex.org/W1995019614","https://openalex.org/W1995948763","https://openalex.org/W2001095912","https://openalex.org/W2003363814","https://openalex.org/W2004666040","https://openalex.org/W2005144917","https://openalex.org/W2005769721","https://openalex.org/W2006071979","https://openalex.org/W2006864691","https://openalex.org/W2007023644","https://openalex.org/W2007618067","https://openalex.org/W2009566905","https://openalex.org/W2010185030","https://openalex.org/W2010815147","https://openalex.org/W2011346704","https://openalex.org/W2012884187","https://openalex.org/W2013502712","https://openalex.org/W2014275438","https://openalex.org/W2016058461","https://openalex.org/W2016130189","https://openalex.org/W2016300311","https://openalex.org/W2016522506","https://openalex.org/W2017774392","https://openalex.org/W2017981046","https://openalex.org/W2018014664","https://openalex.org/W2018781275","https://openalex.org/W2019147967","https://openalex.org/W2019280422","https://openalex.org/W2021400688","https://openalex.org/W2021483168","https://openalex.org/W2022725525","https://openalex.org/W2022844399","https://openalex.org/W2024011126","https://openalex.org/W2024121456","https://openalex.org/W2024619828","https://openalex.org/W2026496498","https://openalex.org/W2027439716","https://openalex.org/W2028100967","https://openalex.org/W2028413481","https://openalex.org/W2029542316","https://openalex.org/W2030519432","https://openalex.org/W2030873172","https://openalex.org/W2036015536","https://openalex.org/W2036214386","https://openalex.org/W2037079949","https://openalex.org/W2037534167","https://openalex.org/W2037568577","https://openalex.org/W2039862570","https://openalex.org/W2039905204","https://openalex.org/W2040448334","https://openalex.org/W2041087801","https://openalex.org/W2041292257","https://openalex.org/W2042118988","https://openalex.org/W2042412790","https://openalex.org/W2042464260","https://openalex.org/W2044084748","https://openalex.org/W2044524123","https://openalex.org/W2045067703","https://openalex.org/W2045651800","https://openalex.org/W2046244841","https://openalex.org/W2049227936","https://openalex.org/W2050135781","https://openalex.org/W2051940183","https://openalex.org/W2052089708","https://openalex.org/W2052593334","https://openalex.org/W2052628347","https://openalex.org/W2053477638","https://openalex.org/W2054593368","https://openalex.org/W2054902966","https://openalex.org/W2057398335","https://openalex.org/W2058444707","https://openalex.org/W2060495746","https://openalex.org/W2062243155","https://openalex.org/W2063226474","https://openalex.org/W2064610868","https://openalex.org/W2070851085","https://openalex.org/W2073109871","https://openalex.org/W2073983610","https://openalex.org/W2074631079","https://openalex.org/W2074708594","https://openalex.org/W2075541265","https://openalex.org/W2075940311","https://openalex.org/W2077119701","https://openalex.org/W2077280771","https://openalex.org/W2077648160","https://openalex.org/W2078875738","https://openalex.org/W2081190990","https://openalex.org/W2081281699","https://openalex.org/W2084361108","https://openalex.org/W2087675944","https://openalex.org/W2088627708","https://openalex.org/W2090774594","https://openalex.org/W2090816622","https://openalex.org/W2092032581","https://openalex.org/W2094197913","https://openalex.org/W2094353708","https://openalex.org/W2095203294","https://openalex.org/W2095988220","https://openalex.org/W2096107882","https://openalex.org/W2096222454","https://openalex.org/W2097801564","https://openalex.org/W2098158472","https://openalex.org/W2098527606","https://openalex.org/W2099384461","https://openalex.org/W2099410750","https://openalex.org/W2102846708","https://openalex.org/W2102881178","https://openalex.org/W2105721795","https://openalex.org/W2106017893","https://openalex.org/W2107629167","https://openalex.org/W2108624165","https://openalex.org/W2113711642","https://openalex.org/W2116585530","https://openalex.org/W2117406280","https://openalex.org/W2121084043","https://openalex.org/W2121279806","https://openalex.org/W2125916741","https://openalex.org/W2129245181","https://openalex.org/W2130381741","https://openalex.org/W2130969726","https://openalex.org/W2131555383","https://openalex.org/W2133675069","https://openalex.org/W2134196477","https://openalex.org/W2138216041","https://openalex.org/W2141028699","https://openalex.org/W2141035582","https://openalex.org/W2142683588","https://openalex.org/W2145142784","https://openalex.org/W2145640271","https://openalex.org/W2145947967","https://openalex.org/W2146347493","https://openalex.org/W2146915535","https://openalex.org/W2147241123","https://openalex.org/W2148152107","https://openalex.org/W2148756953","https://openalex.org/W2151497662","https://openalex.org/W2154101477","https://openalex.org/W2156759569","https://openalex.org/W2164193797","https://openalex.org/W2164320488","https://openalex.org/W2164959697","https://openalex.org/W2166721976","https://openalex.org/W2166798770","https://openalex.org/W2167988140","https://openalex.org/W2168591747","https://openalex.org/W2169613355","https://openalex.org/W2246661972","https://openalex.org/W2278230652","https://openalex.org/W2330893640","https://openalex.org/W2332896732","https://openalex.org/W2340279466","https://openalex.org/W2344004021","https://openalex.org/W2396453915","https://openalex.org/W2399724613","https://openalex.org/W2407013817","https://openalex.org/W2410659073","https://openalex.org/W2414469072","https://openalex.org/W2415570696","https://openalex.org/W2415665603","https://openalex.org/W2432458300","https://openalex.org/W2740242191"],"related_works":["https://openalex.org/W2147518502","https://openalex.org/W7101053950","https://openalex.org/W2130849131","https://openalex.org/W2893411050","https://openalex.org/W2062243155","https://openalex.org/W2145640271","https://openalex.org/W2028413481","https://openalex.org/W7039647478","https://openalex.org/W2037568577","https://openalex.org/W2074708594"],"abstract_inverted_index":{"Human":[0],"equilibrative":[1],"nucleoside":[2,11],"transporter":[3],"1":[4],"is":[5,14,159],"the":[6,36,69,74,83,107,137,148,171,192,214,219,228,236,278,295],"main":[7],"mediator":[8],"of":[9,38,56,71,77,80,139,145,150,164,170,173,186,189,194,231,238,261,272,281,284,299,313],"bi-directional":[10],"flux":[12],"and":[13,18,44,73,116,155,179,218,223,266,320],"found":[15],"ubiquitously.":[16],"Inhibitor":[17],"substrate":[19],"interactions":[20],"with":[21,82,287,301,315],"ENT1":[22],"are":[23],"known":[24],"to":[25,34,50,167,175,198,258,268,305,339,342,345],"be":[26],"affected":[27],"by":[28,68,184,247],"cysteine-modifying":[29],"reagents.":[30],"Our":[31,325],"aim":[32],"was":[33,66,182,245,303],"investigate":[35],"importance":[37,237],"cysteine":[39,57,248,269],"residues":[40,47,252,341],"in":[41,202,227,253,277,292,332,336],"hENT1":[42,81],"function":[43,65,208,230],"identify":[45],"which":[46],"were":[48,255],"sensitive":[49],"thiol":[51,309],"modification":[52,310],"for":[53],"further":[54,234],"application":[55],"scanning":[58,249],"mutagenesis":[59,133,250],"on":[60,134,141,152],"extracellular":[61,241],"loop":[62,217,242],"5.":[63],"Transporter":[64],"assessed":[67],"binding":[70,91,115,127,154,178,201,221,291],"[3H]NBMPR":[72,90,114,126],"cellular":[75],"uptake":[76,271],"[3H]2-chloroadenosine.":[78],"Treatment":[79,283],"neutral":[84],"sulfhydryl-modifier":[85],"methyl":[86],"methanethiosulfonate":[87],"(MMTS)":[88],"enhanced":[89,117],"but":[92,105,294],"decreased":[93,125],"[3H]2-chloroadenosine":[94,118,273],"uptake.":[95,119],"The":[96],"membrane":[97],"impermeable":[98],"positively":[99],"charged":[100,109,195],"reagent":[101,110],"[2-(Trimethylammonium)ethyl]":[102],"methane-thiosulfonate":[103],"(MTSET)":[104],"not":[106],"negatively":[108],"sodium-(2-sulfonatoethyl)-methanethiosulfonate":[111],"(MTSES),":[112],"inhibited":[113,289],"Furthermore,":[120],"all":[121,293],"three":[122],"sulfhydryl":[123,196],"modifiers":[124],"when":[128],"allowed":[129],"cytoplasmic":[130],"access.":[131,347],"Site-directed":[132],"Cys222":[135],"eliminated":[136,183,206],"effect":[138,149,181,193],"MMTS":[140],"NBMPR":[142,177,200,220,290,300],"binding.":[143],"Mutation":[144,163,188,260],"Cys378":[146,158],"abolished":[147,191,270],"MTSET":[151,174,288,302,316,323,344],"NMBPR":[153],"indicated":[156],"that":[157,328],"an":[160,168],"extracellular-located":[161],"residue.":[162],"Cys414":[165],"led":[166],"enhancement":[169],"ability":[172],"inhibit":[176,199],"this":[180,225,239],"co-mutation":[185],"Cys378.":[187],"Cys416":[190],"reagents":[197],"isolated":[203],"membranes,\\nand":[204],"also":[205],"transport":[207,279],"supporting":[209],"a":[210],"conformational":[211],"linkage":[212],"between":[213],"fifth":[215],"intracellular":[216],"domain,":[222],"implicates":[224],"region":[226],"translocation":[229],"hENT1.":[232,282],"To":[233],"confirm":[235],"region,":[240],"5":[243],"(EL5)":[244],"examined":[246],"as":[251],"EL5":[254,285],"individually":[256],"mutated":[257],"cysteines.":[259],"N379,":[262],"F390,":[263],"E391,":[264],"H392,":[265],"D393":[267],"indicating":[274],"their":[275],"role":[276],"mechanism":[280],"mutants":[286],"V389C":[296],"mutant.":[297],"Co-incubation":[298],"able":[304],"protect":[306],"N379C":[307],"from":[308,322],"while":[311],"co-incubation":[312],"adenosine":[314,329],"protected":[317],"R384C,":[318],"Y385C,":[319],"L386C":[321],"effects.":[324],"results":[326],"indicate":[327],"may":[330],"bind":[331],"close":[333],"vicinity":[334],"or":[335],"direct":[337],"contact":[338],"these":[340],"prevent":[343],"attain":[346]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}