{"id":"https://openalex.org/W103969916","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.521.4","title":"A Sensitive Fluorimetric Assay for Quantifying Maleimide Groups in Biopolymers","display_name":"A Sensitive Fluorimetric Assay for Quantifying Maleimide Groups in Biopolymers","publication_year":2010,"publication_date":"2010-04-01","ids":{"openalex":"https://openalex.org/W103969916","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.521.4","mag":"103969916"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.24.1_supplement.521.4","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.521.4","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.24.1_supplement.521.4","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057976112","display_name":"Jinfang Liao","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jinfang Liao","raw_affiliation_strings":["ABD Bioquest, Inc. Sunnyvale CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ABD Bioquest, Inc. Sunnyvale CA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101625662","display_name":"Xing Han","orcid":"https://orcid.org/0000-0002-0343-8089","observed_orcids":["https://orcid.org/0000-0002-0343-8089"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xing Han","raw_affiliation_strings":["ABD Bioquest, Inc. Sunnyvale CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ABD Bioquest, Inc. Sunnyvale CA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110619228","display_name":"Zhenjun J. Diwu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhenjun Diwu","raw_affiliation_strings":["ABD Bioquest, Inc. Sunnyvale CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ABD Bioquest, Inc. Sunnyvale CA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.01167153,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"24","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11481","display_name":"Sulfur Compounds in Biology","score":0.38749998807907104,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11481","display_name":"Sulfur Compounds in Biology","score":0.38749998807907104,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.19539999961853027,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.07689999788999557,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fluorometric-assay","display_name":"fluorometric assay","score":0.8949999809265137},{"id":"https://openalex.org/keywords/thiol-reactivity","display_name":"thiol reactivity","score":0.6959999799728394},{"id":"https://openalex.org/keywords/protein-conjugation","display_name":"protein conjugation","score":0.8270000219345093},{"id":"https://openalex.org/keywords/dna-protein-conjugates","display_name":"protein-DNA conjugates","score":0.7609999775886536},{"id":"https://openalex.org/keywords/microplate-assay","display_name":"microplate assay","score":0.5649999976158142},{"id":"https://openalex.org/keywords/fluorescence-detection","display_name":"fluorescence detection","score":0.3880000114440918}],"concepts":[{"id":"https://openalex.org/C2780105360","wikidata":"https://www.wikidata.org/wiki/Q6743002","display_name":"Maleimide","level":2,"score":0.9982019662857056},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.729711651802063},{"id":"https://openalex.org/C2781324293","wikidata":"https://www.wikidata.org/wiki/Q220410","display_name":"Thiol","level":2,"score":0.6374886631965637},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5572527050971985},{"id":"https://openalex.org/C132538176","wikidata":"https://www.wikidata.org/wiki/Q24768287","display_name":"Semaphorin","level":3,"score":0.507096529006958},{"id":"https://openalex.org/C49853544","wikidata":"https://www.wikidata.org/wiki/Q206229","display_name":"Biomolecule","level":2,"score":0.4376033544540405},{"id":"https://openalex.org/C2779444094","wikidata":"https://www.wikidata.org/wiki/Q727732","display_name":"Thioether","level":2,"score":0.424710214138031},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.41625431180000305},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.1844363510608673},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18367069959640503},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.24.1_supplement.521.4","doi":"https://doi.org/10.1096/fasebj.24.1_supplement.521.4","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.24.1_supplement.521.4","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2749738108","https://openalex.org/W2032778400","https://openalex.org/W2810642653","https://openalex.org/W1489422762","https://openalex.org/W2952176868","https://openalex.org/W1982913130","https://openalex.org/W2125787217","https://openalex.org/W4211242047","https://openalex.org/W2165568934","https://openalex.org/W2313488920"],"abstract_inverted_index":{"Maleimide":[0,103],"groups":[1,40,64,87,99],"rapidly":[2],"and":[3,38,52,67,80,91,144],"selectively":[4],"react":[5],"with":[6,112,149,165],"thiols,":[7],"forming":[8],"stable":[9,24],"thioether":[10],"bonds.":[11],"This":[12,94],"characteristic":[13],"has":[14,107],"led":[15],"to":[16,26,30,147],"their":[17],"widespread":[18],"use":[19],"in":[20,55,88,126,136],"the":[21,44],"formation":[22],"of":[23,36,47,61,97,124],"proteins":[25,27,29,48],"or":[28,140],"other":[31,92],"biomolecules":[32],"conjugates.":[33],"Sensitive":[34],"assays":[35],"maleimide":[37,63,86,98,125],"thiol":[39],"are":[41,50,65],"required":[42],"for":[43,84],"efficient":[45],"conjugation":[46],"that":[49,106],"expensive":[51],"available":[53],"only":[54],"small":[56],"amounts.":[57],"The":[58,115],"existing":[59],"methods":[60],"quantifying":[62,85],"insensitive":[66],"tedious,":[68],"requiring":[69],"a":[70,78,113,127,137,161],"large":[71],"amount":[72],"sample":[73],"size.":[74],"We":[75],"have":[76],"developed":[77],"sensitive":[79],"continuous":[81],"fluorimetric":[82,95],"method":[83,116],"proteins,":[89],"DNA":[90],"biopolymers.":[93],"quantitation":[96],"is":[100],"based":[101],"on":[102],"Green":[104],"dye":[105],"enhanced":[108],"fluorescence":[109,162],"upon":[110],"reacting":[111],"maleimide.":[114],"can":[117,133,156],"detect":[118],"as":[119,121],"little":[120],"10":[122],"picomoles":[123],"0.1":[128],"mL":[129],"assay":[130],"volume.":[131],"It":[132],"be":[134,157],"performed":[135],"convenient":[138],"96\u2010well":[139],"384\u2010well":[141],"microtiter\u2010plate":[142],"format":[143],"easily":[145,158],"adapted":[146],"automation":[148],"no":[150],"separation":[151],"steps":[152],"required.":[153],"Its":[154],"signal":[155],"read":[159],"by":[160],"microplate":[163],"reader":[164],"Ex/Em":[166],"=":[167],"490/520":[168],"nm.":[169]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}
