{"id":"https://openalex.org/G755657400","display_name":"Elucidation of the role of the cytoskeleton in mitochondrial dysfunction in cochlear cells","description":"The mitochondrial movement in the cochlea cell line (HEI-OC1) was continuously tracked by extracting feature points and calculating optical flow using OpenCV, calculating diffusion coefficients, etc. The same coefficients were calculated after exposure to cytoskeletal inhibitors, and dynamic evaluation was performed. The mitochondrial membrane potential was evaluated by plate reader and fluorescence microscopy. The morphology of mitochondria was evaluated by electron microscopy. After exposure to PQQ (0.1, 1.0 nM) for 1 day, the cells were exposed to hydrogen peroxide (100 \u03bcM) for 1 hour to evaluate mitochondrial function and mitochondrial area. SIRT1/PGC-1\u03b1 pathway functions were evaluated.","funder_award_id":"18K16906","funder":{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","doi":"10.13039/501100001691"},"funded_outputs":["https://openalex.org/W2901056768","https://openalex.org/W2990573138","https://openalex.org/W3004710551","https://openalex.org/W3005222164","https://openalex.org/W3005458823","https://openalex.org/W3006286860","https://openalex.org/W3020491331","https://openalex.org/W3113204068","https://openalex.org/W4210847266","https://openalex.org/W4224302139","https://openalex.org/W4253267641","https://openalex.org/W4297013680","https://openalex.org/W4413480959"],"funded_outputs_count":13,"parent_awards":[],"sub_awards":[],"sub_awards_count":0,"amount":4160000.0,"currency":"JPY","amount_usd":39081.48,"amount_usd_basis":"daily_rate","recipients":[],"recipient_countries":[],"recipient_regions":[],"research_scope":{"is_research":true,"basis":"funder_default","tier":1},"funding_type":"fellowship","funder_scheme":"Grant-in-Aid for Early-Career Scientists","start_date":"2018-04-01","end_date":"2021-03-31","start_year":2018,"end_year":2021,"landing_page_url":"https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18K16906/","doi":null,"provenance":"kaken","lead_investigator":{"given_name":"Teru","family_name":"Kamogashira","orcid":null,"role_start":null,"affiliation":{"name":"The University of Tokyo","country":"Japan","ids":[{"id":"https://nrid.nii.ac.jp/nrid/1000030807152","type":"nrid","asserted_by":"kaken"}]},"author":{"id":"https://openalex.org/A5056991066","display_name":"Teru Kamogashira","orcid":"https://orcid.org/0000-0001-8410-930X"},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}]},"co_lead_investigator":null,"investigators":null,"works_api_url":"https://api.openalex.org/works?filter=awards.id:G755657400","primary_topic":null,"topics":null,"institution_awarded":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"updated_date":"2026-11-11T03:01:08.000Z","created_date":"2026-07-13T04:17:19.000Z"}