{"id":"https://openalex.org/G192476754","display_name":"Research on mechanism of hepatitis E virus egress utilizing membrane traffic","description":"We investigated the requirement of the exosomal pathway for the release of hepatitis E virus (HEV) virions by using an inhibitor and accelerator of exosome release or small interfering RNAs against Rab27A and Hrs. These studies revealed that the exosomal pathway is required for the release of HEV virions. Electron microscopic observations revealed the presence of membrane-associated virus particles with a diameter of approximately 50 nm within the multivesicular body (MVB), which possessed internal vesicles in infected cells. These findings indicate that membrane-associated HEV particles are released together with internal vesicles (exosomes) through MVBs by the cellular exosomal pathway.","funder_award_id":"25860343","funder":{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","doi":"10.13039/501100001691"},"funded_outputs":["https://openalex.org/W1566239063","https://openalex.org/W1948808129","https://openalex.org/W1964295995","https://openalex.org/W1972360533","https://openalex.org/W2025897959","https://openalex.org/W2060607285","https://openalex.org/W2062897281","https://openalex.org/W2477762964"],"funded_outputs_count":8,"parent_awards":[],"sub_awards":[],"sub_awards_count":0,"amount":4290000.0,"currency":"JPY","amount_usd":45448.37,"amount_usd_basis":"daily_rate","recipients":[],"recipient_countries":[],"recipient_regions":[],"research_scope":{"is_research":true,"basis":"programme_rule","tier":4},"funding_type":"fellowship","funder_scheme":"Grant-in-Aid for Young Scientists (B)","start_date":"2013-04-01","end_date":"2015-03-31","start_year":2013,"end_year":2015,"landing_page_url":"https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25860343/","doi":null,"provenance":"kaken","lead_investigator":{"given_name":"Shigeo","family_name":"NAGASHIMA","orcid":null,"role_start":null,"affiliation":{"name":"Jichi Medical University","country":"Japan","ids":[{"id":"https://nrid.nii.ac.jp/nrid/1000060433116","type":"nrid","asserted_by":"kaken"}]},"author":{"id":"https://openalex.org/A5028710783","display_name":"Shigeo Nagashima","orcid":"https://orcid.org/0000-0001-8944-713X"},"institutions":[{"id":"https://openalex.org/I146500386","display_name":"Jichi Medical University","ror":"https://ror.org/010hz0g26","country_code":"JP","type":"education","lineage":["https://openalex.org/I146500386"]}]},"co_lead_investigator":null,"investigators":null,"works_api_url":"https://api.openalex.org/works?filter=awards.id:G192476754","primary_topic":null,"topics":null,"institution_awarded":[{"id":"https://openalex.org/I146500386","display_name":"Jichi Medical University","ror":"https://ror.org/010hz0g26","country_code":"JP","type":"education","lineage":["https://openalex.org/I146500386"]}],"updated_date":"2026-11-11T03:01:08.000Z","created_date":"2026-07-13T04:17:19.000Z"}